FastQCFastQC Report
Thu 26 May 2016
SRR1512954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144890
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40440.18854113730774072No Hit
TATCAACGCAGAGTACTTTTTTTTT30000.13986731254283438No Hit
GGTATCAACGCAGAGTACTTTTTTT25100.11702231816083808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.011.7984141
GTATAAT2600.010.9729951
GTACTAA1408.220013E-910.868491
TTATACT2550.010.8215344
GTCATAA1803.45608E-1110.5665871
TTTATAC2700.010.2119823
TGGACCG856.4970844E-410.0752215
ATTATAC2151.0913936E-119.7288173
ATACTTT2950.09.6731486
GTATTAA3450.09.6477541
TAGGACC3750.09.642364
GTATCAA19350.09.6327961
TTATACG800.00445634279.5154864
TATACAG4950.09.4193715
GTCTATA2054.1472958E-109.2779791
CAGGACT4400.09.0829644
CCAGGAC6200.09.0476573
TCTATAC2750.08.9891053
GGACCGT850.00736051948.9524036
GTCCTAC5850.08.9409591
Produced by FastQC (version 0.11.5)