##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512954_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2144890
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.270592431313492	31.0	31.0	34.0	26.0	34.0
2	30.436709108625617	31.0	31.0	34.0	27.0	34.0
3	30.520791742233868	31.0	31.0	34.0	27.0	34.0
4	33.99874445775774	37.0	35.0	37.0	30.0	37.0
5	33.63181841493037	37.0	35.0	37.0	28.0	37.0
6	33.48921296663232	37.0	35.0	37.0	28.0	37.0
7	33.35685979234366	37.0	35.0	37.0	27.0	37.0
8	33.232245476458	37.0	35.0	37.0	27.0	37.0
9	34.669456708735645	39.0	35.0	39.0	27.0	39.0
10	34.55074852323429	39.0	35.0	39.0	27.0	39.0
11	34.58680631640784	39.0	35.0	39.0	27.0	39.0
12	34.42813990461049	39.0	35.0	39.0	26.0	39.0
13	34.40783023838052	39.0	35.0	39.0	26.0	39.0
14	35.45188238091464	40.0	36.0	41.0	26.0	41.0
15	35.444142590062896	40.0	36.0	41.0	26.0	41.0
16	35.41752863783224	40.0	36.0	41.0	26.0	41.0
17	35.383450433355556	40.0	36.0	41.0	26.0	41.0
18	35.30079304766212	40.0	36.0	41.0	25.0	41.0
19	35.29014681405573	40.0	35.0	41.0	25.0	41.0
20	35.252888026891824	40.0	35.0	41.0	25.0	41.0
21	35.20069001207521	40.0	35.0	41.0	25.0	41.0
22	35.15747008005073	40.0	35.0	41.0	25.0	41.0
23	35.04291781862939	40.0	35.0	41.0	24.0	41.0
24	35.01680365892889	40.0	35.0	41.0	24.0	41.0
25	34.91529728797281	40.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6967818161876806
1101	2	0.7091683765287904
1101	3	0.7021938671046151
1101	4	0.5532679969378904
1101	5	0.7312461511065678
1101	6	0.8842738659030047
1101	7	0.8606184426003338
1101	8	0.8270610995313419
1101	9	0.7255970653900548
1101	10	1.0627218465427006
1101	11	1.0467561914709549
1101	12	1.1154929565287617
1101	13	1.1901513837108624
1101	14	1.3396380981298392
1101	15	1.2734150893004212
1101	16	1.3644103633505367
1101	17	1.3135765332003402
1101	18	0.8136505402021328
1101	19	1.1725256167673948
1101	20	1.1165156666823606
1101	21	1.483027275125643
1101	22	1.3465157459322157
1101	23	1.274905669694597
1101	24	1.138306348721862
1101	25	1.280762373772447
1102	1	0.7184804593473011
1102	2	0.6300530234910227
1102	3	0.6453799573301815
1102	4	0.567711433114539
1102	5	0.76162381941527
1102	6	0.7557278240742917
1102	7	0.7631533358679832
1102	8	0.7403005559309648
1102	9	0.9564154907638311
1102	10	0.9069412312112703
1102	11	0.8363411452346696
1102	12	0.8605376804074183
1102	13	1.0397900277347176
1102	14	1.0600322948284813
1102	15	1.0823690474717154
1102	16	1.0886881915417064
1102	17	1.0434016347473118
1102	18	1.1099278714613163
1102	19	1.1573475854822846
1102	20	1.123820026720388
1102	21	1.1786553539433768
1102	22	1.2246464174385707
1102	23	1.2315629378650215
1102	24	1.2391289872146487
1102	25	1.273517429515259
1103	1	0.7582861517392026
1103	2	0.7059264077695779
1103	3	0.7043887885372868
1103	4	0.7033215126901382
1103	5	0.7873911555274375
1103	6	0.8770608732090679
1103	7	0.9067428163109099
1103	8	0.9331463834750693
1103	9	0.9787808648533485
1103	10	1.0284640091995598
1103	11	1.063803673128234
1103	12	1.030271060054595
1103	13	1.01558920461148
1103	14	1.1733514708631745
1103	15	1.0445693232495756
1103	16	1.1895999952552287
1103	17	1.288920507636874
1103	18	1.3608762525821092
1103	19	1.3708596481322814
1103	20	1.3175952888146156
1103	21	0.9594493205132082
1103	22	1.2748821969846915
1103	23	1.3777102053484995
1103	24	1.3002108890293087
1103	25	1.226222841918947
1104	1	0.6455138274390642
1104	2	0.6878410839706888
1104	3	0.5678847366141575
1104	4	0.6058987810464131
1104	5	0.5675327523315019
1104	6	0.5678702467367884
1104	7	0.5904565162642257
1104	8	0.5378513901332553
1104	9	0.753898980510094
1104	10	0.8147071331924991
1104	11	0.7986656151686304
1104	12	0.7685920773486785
1104	13	0.8126953979119875
1104	14	0.9635224755723115
1104	15	0.9664518594286164
1104	16	0.95562553947358
1104	17	0.8344917459742831
1104	18	0.9555674265534222
1104	19	0.9936405990380734
1104	20	0.9288734678048485
1104	21	0.7993340122680195
1104	22	0.9201938626940418
1104	23	0.8569648321993952
1104	24	0.8799279965994273
1104	25	1.0899334010133472
1105	1	0.6397057787433518
1105	2	0.749978981679174
1105	3	0.68801207115132
1105	4	0.6424791450773739
1105	5	0.5740512294036577
1105	6	0.8353953618210994
1105	7	0.8701001999794826
1105	8	1.007831043735841
1105	9	1.0507244338532615
1105	10	1.03372694549698
1105	11	1.0118202393113265
1105	12	0.9975440683284447
1105	13	1.1576979835999879
1105	14	1.286375210043353
1105	15	1.1263948895158507
1105	16	1.227560142761213
1105	17	0.9655924012978332
1105	18	1.2255202059560872
1105	19	1.2736367840885592
1105	20	1.3621112578469834
1105	21	1.1115967012139905
1105	22	1.2242399133735304
1105	23	1.3849674094097395
1105	24	1.3446095330915355
1105	25	1.4158156292829673
1106	1	0.3886203709820606
1106	2	0.41925589536142027
1106	3	0.3955793927744615
1106	4	0.35329071637423937
1106	5	0.4236758567250405
1106	6	0.4633094391945676
1106	7	0.4930410383716648
1106	8	0.5807736781695496
1106	9	0.5433570715198499
1106	10	0.5103101561008145
1106	11	0.45047344764017083
1106	12	0.6594637972459125
1106	13	0.6147624135962815
1106	14	0.7741860748972158
1106	15	0.7288375059891408
1106	16	0.8399246912962468
1106	17	0.8022412272853572
1106	18	0.8073382789232681
1106	19	0.8152636385014347
1106	20	0.8283211312859251
1106	21	0.8147439646453876
1106	22	0.7889321317242803
1106	23	0.820778082685834
1106	24	0.8511411374355617
1106	25	0.8965506892108834
1107	1	0.4472622133588757
1107	2	0.39657819072539624
1107	3	0.33611888361025777
1107	4	0.37881695834675355
1107	5	0.3097148168024475
1107	6	0.4449467643076659
1107	7	0.5097960801532864
1107	8	0.5658856197467799
1107	9	0.5169577542075601
1107	10	0.6432808503683276
1107	11	0.5509426896731924
1107	12	0.37494531504989936
1107	13	0.5026601492104845
1107	14	0.6731139387184442
1107	15	0.7032826635645151
1107	16	0.4948292669031886
1107	17	0.5586272301564037
1107	18	0.5299361476818873
1107	19	0.705265876613737
1107	20	0.7163963081666083
1107	21	0.7290310327986589
1107	22	0.588809014393469
1107	23	0.6240415804280488
1107	24	0.5024402791258424
1107	25	0.4728685840822209
1108	1	0.47907159235222707
1108	2	0.41319136503453535
1108	3	0.4263015501052223
1108	4	0.4898378698961494
1108	5	0.47434959938242827
1108	6	0.3615878404946571
1108	7	0.3560207414509051
1108	8	0.4431228055204528
1108	9	0.41786869273427385
1108	10	0.5145972246431256
1108	11	0.49264245886357116
1108	12	0.44097692507080666
1108	13	0.4638355942371817
1108	14	0.4843446922012262
1108	15	0.34670607538632936
1108	16	0.4493922145302065
1108	17	0.4275662488688212
1108	18	0.5189590701476874
1108	19	0.4423229549404155
1108	20	0.530747366621867
1108	21	0.6511307234015078
1108	22	0.7094165323810984
1108	23	0.6012365831852193
1108	24	0.6971236505972414
1108	25	0.7022838498765012
1109	1	0.11460799081966044
1109	2	0.1951689659776541
1109	3	0.21544095476186698
1109	4	0.35790447761792876
1109	5	0.26425528756398364
1109	6	0.010571041201991704
1109	7	0.1758262233253518
1109	8	0.3192353902537306
1109	9	0.34276553918284947
1109	10	0.37388104427176927
1109	11	0.42484110313117895
1109	12	0.47825219769090666
1109	13	0.4137619131290151
1109	14	0.3834899913465293
1109	15	0.4595413244284714
1109	16	0.3351938649870334
1109	17	0.3348975191311254
1109	18	0.3991904288273176
1109	19	0.6278593534078496
1109	20	0.611730272604774
1109	21	0.7107018993519958
1109	22	0.6538978173796224
1109	23	0.5760597485256227
1109	24	0.1667007210363849
1109	25	0.36356442753638873
1110	1	0.3020198036770303
1110	2	0.3817526142112726
1110	3	0.3518647618768185
1110	4	0.46111531064597244
1110	5	0.3564848562573033
1110	6	0.36181620313452356
1110	7	0.3214559046639991
1110	8	0.4300616208809842
1110	9	0.6411617647413195
1110	10	0.7605995546039708
1110	11	0.5765463678362366
1110	12	0.6675799315146023
1110	13	0.20813794637236782
1110	14	0.6476789191275003
1110	15	0.6868437879869802
1110	16	0.7240352737952804
1110	17	0.6566008209867391
1110	18	0.4646894334428566
1110	19	0.6349143248183893
1110	20	0.7139584928468352
1110	21	0.5549035886245264
1110	22	0.5225012979453325
1110	23	0.6799324173873202
1110	24	0.5309415926196053
1110	25	0.5390710026577281
1111	1	-0.06598777778083331
1111	2	0.10258609628364468
1111	3	0.17882687125792174
1111	4	0.027560377239026934
1111	5	0.35702791012096924
1111	6	0.22451391004550914
1111	7	0.45367346101413375
1111	8	0.6549865538524102
1111	9	0.27626421837248927
1111	10	0.3822741242957548
1111	11	0.5885194748872351
1111	12	0.4603592352797463
1111	13	0.5617931780400056
1111	14	0.7534746619238533
1111	15	0.8323072502460036
1111	16	0.7136799819832476
1111	17	0.9448706044206645
1111	18	0.8138276823708281
1111	19	0.8554541545724845
1111	20	0.8395810257244136
1111	21	0.7181368346229462
1111	22	0.665492128260027
1111	23	0.7238375629317844
1111	24	0.8272842182370326
1111	25	0.9719448931045136
1112	1	0.007231573098415822
1112	2	-0.17907416688616706
1112	3	0.005933986634211408
1112	4	-0.19284445244134218
1112	5	-0.04522698743553377
1112	6	0.04365414325061323
1112	7	-0.0021588856942358348
1112	8	0.2913196382000436
1112	9	0.07454240889074981
1112	10	0.2392511510211861
1112	11	0.0950865664800915
1112	12	0.257648115017858
1112	13	0.15532499951969214
1112	14	0.3415025700487604
1112	15	0.1757911096437752
1112	16	-0.11455220544869604
1112	17	-0.008201548105873258
1112	18	-0.09193085539741475
1112	19	0.02611057924527671
1112	20	0.3951465042968678
1112	21	0.4838243966124196
1112	22	0.3035148463069959
1112	23	0.36856236861445524
1112	24	0.16290571724137948
1112	25	0.2268407390885656
1113	1	-0.29325728052476663
1113	2	-0.7578654200057784
1113	3	-0.37836648900955083
1113	4	-0.39365672608943925
1113	5	-0.3515673675072577
1113	6	-0.5316700081929397
1113	7	-0.2424729967546213
1113	8	-0.14758057413300207
1113	9	-0.20892921428236377
1113	10	-0.5887464020509938
1113	11	-0.33679140629340054
1113	12	-0.4332665129117004
1113	13	-0.34370943921624075
1113	14	-0.41349027091423096
1113	15	-0.34750390917363205
1113	16	-0.6385568333339222
1113	17	-0.3485608741368367
1113	18	-0.2859534362856664
1113	19	-0.3844114763514028
1113	20	-0.008869205688995407
1113	21	-0.31968330988036087
1113	22	-0.44744313480023834
1113	23	-0.19815010406816924
1113	24	-0.4300207995657388
1113	25	-0.4101668277697499
1114	1	-0.7572986822910721
1114	2	-0.593989916354019
1114	3	-0.6129060398840629
1114	4	-0.37493024878673964
1114	5	-0.6071408711044484
1114	6	-1.045827738735838
1114	7	-0.7682348638360281
1114	8	-1.1451759610012218
1114	9	-0.941651889753274
1114	10	-1.0958978477475583
1114	11	-0.9469475724461844
1114	12	-0.9117059428188341
1114	13	-0.8089732481423582
1114	14	-1.9080800277970908
1114	15	-1.2093982764596873
1114	16	-1.1931864275929698
1114	17	-1.3013212041140108
1114	18	-1.468493387353
1114	19	-1.4739638970632
1114	20	-0.7478343434371837
1114	21	-0.9000129232395224
1114	22	-1.040039749532788
1114	23	-0.857651500195395
1114	24	-1.4569965242330767
1114	25	-1.4626402922571842
1115	1	-1.7074719216050802
1115	2	-1.4985382463502468
1115	3	-1.2046398839396595
1115	4	-2.530780630377521
1115	5	-1.6440904662990157
1115	6	-1.5602840806876124
1115	7	-1.1863109498463018
1115	8	-1.1836478567674504
1115	9	-1.7029495886012427
1115	10	-1.5770667052966658
1115	11	-1.4471112357177063
1115	12	-1.9414067640370263
1115	13	-1.465348861154169
1115	14	-2.333717705171516
1115	15	-2.2536825398298745
1115	16	-1.7324824433789345
1115	17	-1.7555070954114171
1115	18	-1.6749133983799567
1115	19	-1.5254301922954951
1115	20	-1.7411601320097745
1115	21	-1.6975748047867825
1115	22	-2.039956757164603
1115	23	-2.415520395468313
1115	24	-1.9880733937377357
1115	25	-1.7417801895323635
1116	1	-1.5192705827140855
1116	2	-1.561830739297914
1116	3	-1.4186586620963695
1116	4	-1.7050560293080963
1116	5	-1.5772198082214572
1116	6	-2.354592530858131
1116	7	-1.694200301873586
1116	8	-1.856125647176274
1116	9	-2.0639173373276165
1116	10	-1.9873037479475073
1116	11	-1.9362700079799282
1116	12	-2.400468698271368
1116	13	-2.0443872174339077
1116	14	-2.5087145474097383
1116	15	-2.306079070502264
1116	16	-2.585488422074569
1116	17	-2.478799798876537
1116	18	-3.3076324417886553
1116	19	-2.762318954873912
1116	20	-2.5034701400094335
1116	21	-2.590027543618831
1116	22	-3.8644119379990975
1116	23	-3.8726582001923227
1116	24	-2.9734775872453767
1116	25	-3.4327073083905155
1201	1	0.7124826728667983
1201	2	0.7367840383045738
1201	3	0.6762440103411329
1201	4	0.7072128236588426
1201	5	0.7692292453758824
1201	6	0.8200564607733085
1201	7	0.8076887096122363
1201	8	0.8949010582296211
1201	9	1.0256759482245315
1201	10	0.917900281100593
1201	11	1.0325873850893714
1201	12	0.9594954816447228
1201	13	1.059668071324026
1201	14	1.0957533204258496
1201	15	1.123182586832634
1201	16	1.172684516531774
1201	17	1.1788516460986926
1201	18	1.1687833683680608
1201	19	1.0735105345713407
1201	20	1.345630643100435
1201	21	1.1814641115346163
1201	22	1.4881744269737283
1201	23	1.3531316279155376
1201	24	1.391375496664672
1201	25	1.232892606880597
1202	1	0.5701853602857803
1202	2	0.6236075073283516
1202	3	0.58540298478934
1202	4	0.570862284696048
1202	5	0.5546860370030373
1202	6	0.6458264511163492
1202	7	0.541697392176637
1202	8	0.45201197407486404
1202	9	0.7518551848896564
1202	10	0.675145089396878
1202	11	0.7645050312270243
1202	12	0.6649424423121815
1202	13	0.8024764198097927
1202	14	0.7439604317766921
1202	15	0.8598825163629229
1202	16	0.696355466480874
1202	17	0.7939078043684447
1202	18	0.8525017331227644
1202	19	0.9299909640015755
1202	20	0.9496033948121507
1202	21	0.8933486108081681
1202	22	0.9473471473655763
1202	23	0.9536071679658207
1202	24	0.8834316462344631
1202	25	1.02569982437101
1203	1	0.6114465323622369
1203	2	0.4491811063202462
1203	3	0.5581830405119277
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2211	16	-0.2853860886601396
2211	17	-0.01849597157927718
2211	18	-0.2579610739654754
2211	19	-0.26405236363360274
2211	20	-0.4232687130497865
2211	21	-0.31918048119209885
2211	22	-0.12268216671103005
2211	23	-0.3113919978937858
2211	24	-0.49673132529860453
2211	25	-0.49243447439857846
2212	1	-0.1602361492473534
2212	2	-0.09061335281559835
2212	3	-0.08260349214717166
2212	4	0.12970330303323863
2212	5	0.012785259601102439
2212	6	0.003804188766508787
2212	7	-0.14732140280797523
2212	8	-0.4047705822804204
2212	9	-0.4245949621978369
2212	10	-0.41662148655876763
2212	11	-0.2704097258454681
2212	12	-0.1758008794067507
2212	13	-0.15124510583090256
2212	14	-0.17645870401007357
2212	15	-0.16193263661655521
2212	16	-0.16065123935260317
2212	17	-0.3430603280866009
2212	18	-0.1438177731379895
2212	19	-0.11660711835046556
2212	20	-0.39921278396486315
2212	21	-0.18224766580110696
2212	22	-0.23191571961137214
2212	23	-0.8130581422743504
2212	24	-0.4553323948991732
2212	25	-0.6617105031212134
2213	1	-0.15367006454740917
2213	2	-0.29025588271904823
2213	3	-0.24493917121569098
2213	4	-0.17252410653960482
2213	5	-0.12946571120914285
2213	6	0.09482453163030158
2213	7	-0.49275272512528545
2213	8	-0.19507245284320618
2213	9	-0.37791452031036243
2213	10	-0.12804151859706536
2213	11	-0.15369953114321078
2213	12	-0.46455070020421374
2213	13	-0.2832373809031594
2213	14	-0.17556113306145704
2213	15	-0.33803782278980776
2213	16	-0.6009845872299096
2213	17	-0.43681441857920333
2213	18	-0.6452067226267673
2213	19	-0.8499677735772622
2213	20	-0.34574030635738495
2213	21	-1.0341832609301207
2213	22	-0.42946807272243603
2213	23	-0.4853491713436
2213	24	-0.7014169494068696
2213	25	-0.6185533388636557
2214	1	-1.1746839636863307
2214	2	-1.095175748288728
2214	3	-0.8641996616273921
2214	4	-1.5399810766028637
2214	5	-1.482549488105029
2214	6	-2.973719765708548
2214	7	-1.4998348794307432
2214	8	-2.047443973806555
2214	9	-1.5402813411362786
2214	10	-1.751064374119423
2214	11	-1.5894668252904438
2214	12	-1.631887056584759
2214	13	-1.879221666921083
2214	14	-2.0175350329220265
2214	15	-2.436979004544554
2214	16	-1.83238479406603
2214	17	-1.9268446208617718
2214	18	-1.8345992397298403
2214	19	-3.690807607890367
2214	20	-2.398624651592158
2214	21	-2.897085457666485
2214	22	-2.6450510297722687
2214	23	-2.573030037837711
2214	24	-1.8355915904270574
2214	25	-1.7753323333650002
2215	1	-1.5466910594972028
2215	2	-1.306367931146756
2215	3	-1.4044578332362825
2215	4	-1.240621900218791
2215	5	-1.9406555757551196
2215	6	-1.8713696144271736
2215	7	-1.9744580949602728
2215	8	-2.8432175551049212
2215	9	-2.0884953645735607
2215	10	-1.6893603937040993
2215	11	-2.3503520391480848
2215	12	-3.1351207488410537
2215	13	-2.48457100814883
2215	14	-2.53723835513766
2215	15	-3.5362568270487102
2215	16	-2.9684306868153243
2215	17	-2.7207218701048674
2215	18	-3.2733095467287114
2215	19	-3.8363050888575927
2215	20	-3.1715746358839922
2215	21	-2.530182944409141
2215	22	-3.3680076109974486
2215	23	-3.330839736474857
2215	24	-2.2787888078654674
2215	25	-2.458133157259738
2216	1	-1.589152878830287
2216	2	-1.371151077172641
2216	3	-1.765074252329267
2216	4	-1.3924021647152287
2216	5	-1.370769728034226
2216	6	-1.7925497397487966
2216	7	-3.0651483281343417
2216	8	-2.1428847247937384
2216	9	-2.2845087934971744
2216	10	-2.170119394666756
2216	11	-2.8372279823146194
2216	12	-2.2573769880031804
2216	13	-2.8385497847925336
2216	14	-2.6686399548445294
2216	15	-2.911377319335436
2216	16	-2.739264080414891
2216	17	-2.710297258339935
2216	18	-2.6418245385566124
2216	19	-2.717930959302869
2216	20	-3.258179622070969
2216	21	-2.9252642556094983
2216	22	-3.403074844005989
2216	23	-2.4269111816912456
2216	24	-2.5680898642119843
2216	25	-2.4612628228705447
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61859.0
3	1.0
4	958.0
5	8658.0
6	10032.0
7	7653.0
8	6163.0
9	5563.0
10	4878.0
11	4330.0
12	4017.0
13	3811.0
14	3575.0
15	3690.0
16	3802.0
17	3922.0
18	4447.0
19	5220.0
20	6654.0
21	8439.0
22	10762.0
23	13025.0
24	15317.0
25	17540.0
26	19923.0
27	21843.0
28	24805.0
29	29947.0
30	37936.0
31	47992.0
32	61018.0
33	78210.0
34	101735.0
35	133504.0
36	182547.0
37	282742.0
38	908372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.05894026046257	18.791267397571936	13.4760989125074	27.67369342945809
2	14.394511225999318	20.45459737505985	38.41130092168026	26.73959047726057
3	16.961522502319465	24.418781382728252	29.979346260181174	28.64034985477111
4	11.87340531258555	17.091458048557314	36.69295583634024	34.3421808025169
5	13.464600838816809	37.549579915054515	35.36832027143735	13.617498974691323
6	32.53479323298623	36.788650036091546	17.555797254615964	13.12075947630626
7	28.03053446507552	31.592873177122566	22.05045639855807	18.32613595924385
8	25.820106560665863	35.634231914079635	20.149203594322984	18.39645793093152
9	26.330365226834147	14.940029135302835	19.357763886498265	39.371841751364755
10	14.796252700046114	28.562800692257284	34.201626468573046	22.43932013912355
11	34.91831773417619	22.257123594374047	23.78623575257813	19.03832291887164
12	22.88247700146368	24.674938321630258	31.082541069564247	21.360043607341815
13	28.687272584853474	21.355622737504873	25.652941406854673	24.30416327078698
14	21.7318710298292	20.74149642101808	27.145524127821314	30.381108421331405
15	23.60667646433487	29.086765314762523	23.28365181971793	24.02290640118467
16	23.442147014579472	27.552993161179973	25.69323140541342	23.311628418827134
17	22.643783116775314	27.541498274966354	26.819912829605475	22.99480577865286
18	22.592694990854866	27.313802272132826	27.56943703006774	22.524065706944565
19	23.669988945423214	26.7511899638803	27.016481157098543	22.56233993359794
20	23.92592613314758	26.51621752927343	27.03477935711761	22.523076980461383
21	23.77559812092402	26.161461412365423	26.782147659267352	23.2807928074432
22	23.77003665937644	26.496994471954388	26.687447928346607	23.045520940322564
23	23.476635165439717	26.39953564052236	26.675260735981798	23.448568458056123
24	23.350195114901	26.45832653422786	26.922359654807472	23.269118696063668
25	23.440792835535863	26.296899302874664	26.886042946501505	23.376264915087972
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	182.0
2	336.0
3	490.0
4	490.0
5	490.0
6	1093.5
7	1697.0
8	1697.0
9	1697.0
10	2614.5
11	3532.0
12	3532.0
13	3532.0
14	6078.0
15	8624.0
16	8624.0
17	8624.0
18	15520.5
19	22417.0
20	22417.0
21	22417.0
22	35067.5
23	47718.0
24	47718.0
25	47718.0
26	66385.0
27	85052.0
28	85052.0
29	85052.0
30	108093.5
31	131135.0
32	131135.0
33	131135.0
34	153688.5
35	176242.0
36	176242.0
37	176242.0
38	198798.5
39	221355.0
40	221355.0
41	221355.0
42	239140.5
43	256926.0
44	256926.0
45	256926.0
46	268058.5
47	279191.0
48	279191.0
49	279191.0
50	277193.0
51	275195.0
52	275195.0
53	275195.0
54	254609.0
55	234023.0
56	234023.0
57	234023.0
58	204200.0
59	174377.0
60	174377.0
61	174377.0
62	144483.0
63	114589.0
64	114589.0
65	114589.0
66	89209.0
67	63829.0
68	63829.0
69	63829.0
70	46934.0
71	30039.0
72	30039.0
73	30039.0
74	21073.5
75	12108.0
76	12108.0
77	12108.0
78	8253.5
79	4399.0
80	4399.0
81	4399.0
82	2862.5
83	1326.0
84	1326.0
85	1326.0
86	844.0
87	362.0
88	362.0
89	362.0
90	219.0
91	76.0
92	76.0
93	76.0
94	41.0
95	6.0
96	6.0
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07939801108681563
2	1.3986731254283437E-4
3	0.0
4	0.030817431196937837
5	0.07613444046081617
6	0.08061019446218687
7	0.038277021199222334
8	0.07039988064655997
9	0.05123805882819166
10	0.10881676915832512
11	0.0054082027516562615
12	0.013846863941740603
13	0.0062474066269132674
14	0.006806875877084605
15	0.007552834877313056
16	0.01715705700525435
17	0.02722750350833842
18	0.001538540437971178
19	0.003589927688599416
20	0.00522171300159915
21	2.7973462508566873E-4
22	4.196019376285031E-4
23	0.0
24	0.0
25	0.003823039876170806
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2144890.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.05271447586384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57165064377763	58.77458853161024
2	12.141088477454993	17.4959676298453
3	3.2536200392946566	7.032964671127402
4	1.2423363999551063	3.580548396357514
5	0.6121265177807244	2.205268860437966
6	0.32481583941021397	1.4042317760557312
7	0.214193695047377	1.0803266006245233
8	0.1372791370087318	0.7913067569906516
9	0.09466976551218251	0.613909222555164
>10	0.38937674373508446	4.58595448618215
>50	0.010996998587216497	0.5376361178652843
>100	0.0066150574326461434	0.8727833019185336
>500	9.068205287607986E-4	0.45401187918751135
>1k	3.2386447455734935E-4	0.5705017692420947
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4044	0.18854113730774072	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3000	0.13986731254283438	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2510	0.11702231816083808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.324487502855625E-5	0.0
10	0.0	0.0	0.0	9.324487502855625E-5	4.6622437514278125E-5
11	0.0	0.0	0.0	9.324487502855625E-5	4.6622437514278125E-5
12	0.0	0.0	0.0	9.324487502855625E-5	4.6622437514278125E-5
13	0.0	4.6622437514278125E-5	0.0	9.324487502855625E-5	4.6622437514278125E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	935	0.0	11.798414	1
GTATAAT	260	0.0	10.972995	1
GTACTAA	140	8.220013E-9	10.86849	1
TTATACT	255	0.0	10.821534	4
GTCATAA	180	3.45608E-11	10.566587	1
TTTATAC	270	0.0	10.211982	3
TGGACCG	85	6.4970844E-4	10.075221	5
ATTATAC	215	1.0913936E-11	9.728817	3
ATACTTT	295	0.0	9.673148	6
GTATTAA	345	0.0	9.647754	1
TAGGACC	375	0.0	9.64236	4
GTATCAA	1935	0.0	9.632796	1
TTATACG	80	0.0044563427	9.515486	4
TATACAG	495	0.0	9.419371	5
GTCTATA	205	4.1472958E-10	9.277979	1
CAGGACT	440	0.0	9.082964	4
CCAGGAC	620	0.0	9.047657	3
TCTATAC	275	0.0	8.989105	3
GGACCGT	85	0.0073605194	8.952403	6
GTCCTAC	585	0.0	8.940959	1
>>END_MODULE