FastQCFastQC Report
Thu 26 May 2016
SRR1512953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936590
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29470.15217469882628742No Hit
GTCCTACAGTGGACATTTCTAAATT19900.10275794050366882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC905.444472E-712.65950410
TGCGCCA1155.302354E-912.39518212
ATTATAC1800.011.6078433
GGCGTGC907.51239E-611.6042468
TACGCTG752.0807443E-411.3962035
TGCACCG752.0807443E-411.3962035
TAGGACC4350.011.1347964
GGTATCA9050.010.9511131
GGACGTG10450.010.5392196
AGGACGT11100.010.43796355
GTAGGAC11950.010.2523433
GTATAAA3350.010.2407351
GTATCAA17700.010.22948551
GTCCTAC10550.010.2070311
TAGGACG11050.010.1419414
GACGTGG6300.010.0972017
ACGTGGA6300.010.0972018
CTAGGAC2450.010.0787953
TCCAACG1701.551598E-910.0617118
TGTAGGA12700.010.021062
Produced by FastQC (version 0.11.5)