##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512953_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1936590 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.94111092177487 31.0 30.0 34.0 23.0 34.0 2 28.9593904750102 31.0 30.0 34.0 22.0 34.0 3 29.13019895796219 31.0 30.0 34.0 22.0 34.0 4 32.59914488869611 35.0 33.0 37.0 25.0 37.0 5 32.707453823473216 35.0 33.0 37.0 26.0 37.0 6 32.796423094201664 36.0 35.0 37.0 26.0 37.0 7 32.59133063787379 36.0 33.0 37.0 25.0 37.0 8 32.59020081689981 36.0 33.0 37.0 25.0 37.0 9 33.92499961272133 38.0 34.0 39.0 23.0 39.0 10 33.79010322267491 38.0 34.0 39.0 23.0 39.0 11 33.868694457784045 38.0 34.0 39.0 23.0 39.0 12 33.763257065253875 38.0 34.0 39.0 22.0 39.0 13 33.75731053036523 38.0 34.0 39.0 22.0 39.0 14 34.73265275561683 39.0 34.0 41.0 22.0 41.0 15 34.67742268626813 39.0 34.0 41.0 19.0 41.0 16 34.55424586515473 39.0 34.0 41.0 18.0 41.0 17 34.48962506261005 39.0 34.0 41.0 18.0 41.0 18 34.16940911602352 39.0 33.0 41.0 17.0 41.0 19 34.41870297791479 39.0 34.0 41.0 18.0 41.0 20 34.51750706138109 39.0 34.0 41.0 18.0 41.0 21 34.44868867442257 39.0 34.0 41.0 18.0 41.0 22 34.353711420589796 39.0 34.0 41.0 18.0 41.0 23 34.07386488621753 39.0 34.0 41.0 10.0 41.0 24 34.08211547100832 39.0 34.0 41.0 8.0 41.0 25 33.992720193742606 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4622554002920083 1101 2 0.6347017335260965 1101 3 0.6615050815242007 1101 4 0.6704188947515703 1101 5 0.5028369174560794 1101 6 0.801954482572306 1101 7 0.7134809809331344 1101 8 0.5997299015310986 1101 9 0.36265937144471394 1101 10 0.5423915478839163 1101 11 0.6933499463619768 1101 12 0.9580850377160246 1101 13 0.7400734986123467 1101 14 0.6393447278312081 1101 15 0.4756453035612722 1101 16 1.0467615086617528 1101 17 0.9578201252070855 1101 18 1.162611688198794 1101 19 0.6740081780899843 1101 20 0.8870424737530342 1101 21 0.9066444480245792 1101 22 0.918149062736596 1101 23 1.1355386849207036 1101 24 0.6383621300569828 1101 25 0.9441300769307901 1102 1 0.7682090728855613 1102 2 0.7571028306653176 1102 3 0.6798138928310955 1102 4 0.8341992209202189 1102 5 0.7602744999767594 1102 6 0.6371762211689855 1102 7 0.8929499170923307 1102 8 0.8177593880724388 1102 9 0.8397092885512407 1102 10 1.0039483268499936 1102 11 0.9648934064241033 1102 12 1.0693235478205665 1102 13 0.880168555035624 1102 14 1.049172210919025 1102 15 1.176429109845543 1102 16 1.1336978162805735 1102 17 0.9309855739219088 1102 18 1.1945540541734232 1102 19 1.2230791261642864 1102 20 1.173062872645076 1102 21 0.9755760758785428 1102 22 1.286823537025981 1102 23 1.4837100001029384 1102 24 1.324048250122246 1102 25 1.1136354973154852 1103 1 0.3879063052453162 1103 2 0.5955991012290021 1103 3 0.5300851859650173 1103 4 0.6356846915406393 1103 5 0.2711695646931176 1103 6 0.5869858656961853 1103 7 0.4514971142177444 1103 8 0.4705810618047366 1103 9 0.9060698405033136 1103 10 0.7085722425217256 1103 11 0.6836258033270894 1103 12 0.7880525676305936 1103 13 0.8283609097474596 1103 14 1.0101639730636123 1103 15 1.088451560759502 1103 16 1.1295593014360037 1103 17 0.8871569861546433 1103 18 1.2436675672731639 1103 19 1.2840195564959842 1103 20 1.2877131521558027 1103 21 1.0253762645454643 1103 22 1.1142133368544265 1103 23 1.416437070204637 1103 24 1.255053002817725 1103 25 1.231446623260787 1104 1 0.7499014524483023 1104 2 0.7305496639139193 1104 3 0.7566875183239468 1104 4 1.0459633946816496 1104 5 0.746715947939876 1104 6 0.830344320608198 1104 7 1.0250784251826701 1104 8 0.9613858099161803 1104 9 1.1573067714418883 1104 10 1.1833709667772823 1104 11 1.177380377775826 1104 12 1.0813653335031006 1104 13 1.1473216393526258 1104 14 1.249438138351472 1104 15 1.344898816533842 1104 16 1.2711647166961413 1104 17 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1107 17 1.263788682578344 1107 18 1.539110609662508 1107 19 1.4718471436800087 1107 20 1.3723263905688654 1107 21 1.3306760764341874 1107 22 1.3242002366366705 1107 23 1.3149495088722887 1107 24 1.358271057141124 1107 25 1.3498936262317685 1108 1 0.5510139966161738 1108 2 0.6108024225719788 1108 3 0.45852820857850674 1108 4 0.5863690987139734 1108 5 0.4993977178159028 1108 6 0.6723551671963577 1108 7 0.6151087049914068 1108 8 0.6499364098367622 1108 9 0.7783947370919293 1108 10 0.7778228525509974 1108 11 0.7337310632800254 1108 12 0.8961834640365325 1108 13 0.8187985649732639 1108 14 1.0736950965178806 1108 15 1.0491365333788636 1108 16 1.0500754231721814 1108 17 1.0861913783219066 1108 18 1.2377756196595655 1108 19 1.1474923955135026 1108 20 1.0505507343085867 1108 21 0.9783372444199401 1108 22 0.9231591279786571 1108 23 1.0445329959485221 1108 24 1.1239876155089448 1108 25 1.2889105108327215 1109 1 0.12341512398021592 1109 2 -0.09417965701765141 1109 3 0.16426653223995302 1109 4 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-3.4256114530006805 2215 18 -2.2776390336953156 2215 19 -2.8754689482350635 2215 20 -3.121102604387424 2215 21 -2.729020362988532 2215 22 -2.367280807021846 2215 23 -2.9020078252897896 2215 24 -2.6575249774223018 2215 25 -2.746619374943684 2216 1 -1.9471788915160246 2216 2 -1.8060029135089195 2216 3 -2.433669038803636 2216 4 -3.1486756642385814 2216 5 -2.233145069335613 2216 6 -2.190731027679643 2216 7 -2.058429064662576 2216 8 -2.544126831882334 2216 9 -2.848112406481814 2216 10 -2.3550068543531495 2216 11 -2.915128136502563 2216 12 -3.500177040600196 2216 13 -4.148789831751497 2216 14 -3.385246974731178 2216 15 -3.173008905050011 2216 16 -2.473109919453094 2216 17 -2.505871214039118 2216 18 -2.514466126106168 2216 19 -2.843805613700269 2216 20 -2.820171767557156 2216 21 -3.2029900705691006 2216 22 -3.8161646676081595 2216 23 -3.621992214510801 2216 24 -3.657312692280005 2216 25 -3.64999924490451 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95719.0 3 3.0 4 789.0 5 4684.0 6 5611.0 7 4973.0 8 4659.0 9 4453.0 10 4062.0 11 3762.0 12 3684.0 13 3694.0 14 3681.0 15 3784.0 16 3985.0 17 4094.0 18 4569.0 19 5499.0 20 7007.0 21 9040.0 22 11230.0 23 14004.0 24 16035.0 25 18234.0 26 20529.0 27 22815.0 28 26022.0 29 30625.0 30 37691.0 31 46878.0 32 59106.0 33 75822.0 34 98629.0 35 129325.0 36 176576.0 37 276082.0 38 699235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42615711014998 18.417580549708294 12.402431908328747 25.753830431812975 2 15.130979113492158 20.208508644554335 40.56145048952833 24.099061752425172 3 18.823382347016047 24.493445500386894 29.561418280281952 27.121753872315104 4 12.41569258249691 16.76747139991766 38.0245371582828 32.792298859302626 5 13.384412093185677 37.578118784978464 35.69045692967476 13.347012192161104 6 31.99733506175581 36.914094476192446 18.119514216877423 12.969056245174318 7 27.939974941355956 31.406558423515875 22.410185006336967 18.243281628791205 8 25.28105991623596 36.27295983323632 20.68746633143145 17.75851391909627 9 26.481930909246792 15.126791711836596 19.882429926139096 38.508847452777516 10 14.88454800497465 28.635028323286242 34.63601168464675 21.84441198709235 11 34.40174813671702 22.342610625890583 24.55267522038928 18.70296601700312 12 23.10395441826162 24.574246441767283 31.619232171650445 20.702566968320653 13 28.866989425799318 21.787274393122278 25.664877271304633 23.680858909773775 14 21.28586429404111 21.61260648432944 27.50171351124926 29.599815710380184 15 23.269022276697147 30.029095187698307 23.397014243884094 23.30486829172045 16 22.824703697352955 27.849349962503794 26.898051643531957 22.427894696611293 17 22.194699315399255 28.034026025674464 27.398956184002916 22.372318474923368 18 22.10901444097067 27.379431128314557 28.748624964848723 21.762929465866048 19 23.579150160371462 26.998703599448383 27.199413262539192 22.222732977640963 20 23.665134603435963 27.11519500622105 27.534734829449288 21.684935560893702 21 23.43016490273543 26.58969449582178 26.865401828717467 23.114738772725325 22 23.43076569492541 27.399023352262013 26.896341573637223 22.273869379175352 23 22.95092127519465 27.222745877826128 27.126783477733156 22.699549369246064 24 23.211212729079428 26.752531210167398 27.59705985038952 22.439196210363654 25 23.095238341182146 27.025050434516736 27.319135573558988 22.560575650742134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 268.0 1 268.0 2 416.5 3 565.0 4 565.0 5 565.0 6 1064.5 7 1564.0 8 1564.0 9 1564.0 10 2467.0 11 3370.0 12 3370.0 13 3370.0 14 5846.5 15 8323.0 16 8323.0 17 8323.0 18 14896.0 19 21469.0 20 21469.0 21 21469.0 22 34026.0 23 46583.0 24 46583.0 25 46583.0 26 65935.5 27 85288.0 28 85288.0 29 85288.0 30 109896.0 31 134504.0 32 134504.0 33 134504.0 34 154249.0 35 173994.0 36 173994.0 37 173994.0 38 191729.5 39 209465.0 40 209465.0 41 209465.0 42 222337.5 43 235210.0 44 235210.0 45 235210.0 46 242218.0 47 249226.0 48 249226.0 49 249226.0 50 244012.5 51 238799.0 52 238799.0 53 238799.0 54 217436.5 55 196074.0 56 196074.0 57 196074.0 58 170697.0 59 145320.0 60 145320.0 61 145320.0 62 119695.0 63 94070.0 64 94070.0 65 94070.0 66 73249.0 67 52428.0 68 52428.0 69 52428.0 70 38672.5 71 24917.0 72 24917.0 73 24917.0 74 17374.5 75 9832.0 76 9832.0 77 9832.0 78 6717.5 79 3603.0 80 3603.0 81 3603.0 82 2399.5 83 1196.0 84 1196.0 85 1196.0 86 778.5 87 361.0 88 361.0 89 361.0 90 234.5 91 108.0 92 108.0 93 108.0 94 72.5 95 37.0 96 37.0 97 37.0 98 26.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35691602249314514 2 0.038572955555899804 3 0.034493620229372246 4 0.03454525738540424 5 0.039037689960187756 6 0.017298447270718116 7 0.017143535802622137 8 0.022565437185981543 9 0.01977703076025385 10 0.0193122963559659 11 0.018434464703421995 12 0.01941557066802989 13 0.019002473419773933 14 0.02618003810822115 15 0.027419329852989015 16 0.020345039476605785 17 0.021945791313597612 18 0.10812820473099624 19 0.023753091774717416 20 0.021739242689469637 21 0.02473419773932531 22 0.019363933511997892 23 0.02142941975327767 24 0.018692650483581964 25 0.021481056909309664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1936590.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.36081394463311 #Duplication Level Percentage of deduplicated Percentage of total 1 83.67419365572131 62.22081146400292 2 11.108914156946422 16.521357975031876 3 2.879906011842256 6.424564653738971 4 0.9791059185875417 2.912284521767091 5 0.47899458751140267 1.780921370121085 6 0.24406202730767423 1.0889190602145558 7 0.15694967262948956 0.8169633783550784 8 0.10232452589124785 0.60871480254175 9 0.06589388639003611 0.4409930723344441 >10 0.2829284008816221 3.521947653840147 >50 0.014955278724256463 0.747351387721611 >100 0.009822410757519036 1.4502582870194085 >500 0.001392476292296903 0.7004864608905707 >1k 5.56990516918749E-4 0.7644259124204805 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2947 0.15217469882628742 No Hit GTCCTACAGTGGACATTTCTAAATT 1990 0.10275794050366882 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.163715603199438E-5 0.0 5 0.0 0.0 0.0 5.163715603199438E-5 0.0 6 0.0 0.0 0.0 5.163715603199438E-5 5.163715603199438E-5 7 0.0 0.0 0.0 5.163715603199438E-5 5.163715603199438E-5 8 5.163715603199438E-5 0.0 0.0 5.163715603199438E-5 5.163715603199438E-5 9 5.163715603199438E-5 0.0 0.0 5.163715603199438E-5 5.163715603199438E-5 10 5.163715603199438E-5 0.0 0.0 5.163715603199438E-5 5.163715603199438E-5 11 5.163715603199438E-5 0.0 0.0 5.163715603199438E-5 5.163715603199438E-5 12 5.163715603199438E-5 0.0 0.0 5.163715603199438E-5 1.0327431206398876E-4 13 5.163715603199438E-5 0.0 0.0 5.163715603199438E-5 1.5491146809598315E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 90 5.444472E-7 12.659504 10 TGCGCCA 115 5.302354E-9 12.395182 12 ATTATAC 180 0.0 11.607843 3 GGCGTGC 90 7.51239E-6 11.604246 8 TACGCTG 75 2.0807443E-4 11.396203 5 TGCACCG 75 2.0807443E-4 11.396203 5 TAGGACC 435 0.0 11.134796 4 GGTATCA 905 0.0 10.951113 1 GGACGTG 1045 0.0 10.539219 6 AGGACGT 1110 0.0 10.4379635 5 GTAGGAC 1195 0.0 10.252343 3 GTATAAA 335 0.0 10.240735 1 GTATCAA 1770 0.0 10.2294855 1 GTCCTAC 1055 0.0 10.207031 1 TAGGACG 1105 0.0 10.141941 4 GACGTGG 630 0.0 10.097201 7 ACGTGGA 630 0.0 10.097201 8 CTAGGAC 245 0.0 10.078795 3 TCCAACG 170 1.551598E-9 10.06171 18 TGTAGGA 1270 0.0 10.02106 2 >>END_MODULE