FastQCFastQC Report
Thu 26 May 2016
SRR1512953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936590
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36650.1892501768572594No Hit
TATCAACGCAGAGTACTTTTTTTTT26140.13497952586763332No Hit
GGTATCAACGCAGAGTACTTTTTTT23270.12015966208645092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.00521703414.2756775
TAGGCTC905.290476E-712.689495
TAGGACC4400.012.3289934
CCGGAAC752.0566628E-411.4104943
GTATTAC2250.010.9881691
GTAGGAC10900.010.6428173
AGGACGT10200.010.5434745
GGTATCA9150.010.4962641
GTATCAA18900.010.3139861
AGGACCT8600.010.2917675
GGACGTG10550.010.281776
TAAGACA3150.010.2724454
TGTAGGA11850.010.1905442
CTAGTAT1409.5602445E-810.1879423
CTATACA2151.8189894E-1210.1811034
GATGCGA750.002613303310.1494896
GACGTGA6650.010.1472067
ACGAAAT750.002669398210.1235412
TATGGCG10600.010.11757116
TTAGGAC7050.010.1156873
Produced by FastQC (version 0.11.5)