##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512953_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1936590 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.33457417419278 31.0 31.0 34.0 26.0 34.0 2 30.45067877041604 31.0 31.0 34.0 27.0 34.0 3 30.55892574060591 31.0 31.0 34.0 27.0 34.0 4 34.046778616020944 37.0 35.0 37.0 30.0 37.0 5 33.68521370037024 37.0 35.0 37.0 28.0 37.0 6 33.544055788783375 37.0 35.0 37.0 28.0 37.0 7 33.412331469232 37.0 35.0 37.0 27.0 37.0 8 33.29217748723271 37.0 35.0 37.0 27.0 37.0 9 34.729395483814336 39.0 35.0 39.0 27.0 39.0 10 34.60721267795455 39.0 35.0 39.0 27.0 39.0 11 34.65101028095776 39.0 35.0 39.0 27.0 39.0 12 34.48980992362865 39.0 35.0 39.0 27.0 39.0 13 34.47756985216282 39.0 35.0 39.0 27.0 39.0 14 35.50830325468994 40.0 36.0 41.0 27.0 41.0 15 35.517444580422286 40.0 36.0 41.0 27.0 41.0 16 35.48003965733583 40.0 36.0 41.0 27.0 41.0 17 35.45300140969436 40.0 36.0 41.0 26.0 41.0 18 35.36956609297786 40.0 36.0 41.0 26.0 41.0 19 35.36160054528837 40.0 35.0 41.0 25.0 41.0 20 35.331299862128795 40.0 35.0 41.0 25.0 41.0 21 35.270260612726496 40.0 35.0 41.0 25.0 41.0 22 35.22403141604573 40.0 35.0 41.0 25.0 41.0 23 35.113125132320214 40.0 35.0 41.0 25.0 41.0 24 35.0926773349031 40.0 35.0 41.0 25.0 41.0 25 35.00054219013833 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4533081321472352 1101 2 0.5500157997072996 1101 3 0.5057128521858552 1101 4 0.2401673395853905 1101 5 0.5829102086154734 1101 6 0.5666785960024114 1101 7 0.49872934286807435 1101 8 0.44646943202889844 1101 9 0.39929667737646923 1101 10 0.7762715394712387 1101 11 0.7296277222922711 1101 12 0.7443741237953176 1101 13 0.6723781772317636 1101 14 0.7783604605163461 1101 15 0.7733516640184064 1101 16 0.8440051581125516 1101 17 0.898811318550635 1101 18 0.2999710985803503 1101 19 0.6564359986491723 1101 20 0.6172036852455989 1101 21 0.748991979481417 1101 22 0.608278152368193 1101 23 0.6572339241604581 1101 24 0.6818457261090245 1101 25 0.7310217252941484 1102 1 0.5878363444802979 1102 2 0.5810745543146432 1102 3 0.5382175898640185 1102 4 0.5267617853180795 1102 5 0.6029367678184769 1102 6 0.5378603117666856 1102 7 0.6842161764344894 1102 8 0.5920476414605176 1102 9 0.7810557436984169 1102 10 0.8767429144625396 1102 11 0.7494272520963534 1102 12 0.7709119416451458 1102 13 0.7840887790191857 1102 14 0.8813796740929831 1102 15 0.9107976202710901 1102 16 0.8966003792593042 1102 17 0.8250868735184511 1102 18 0.8936534391378572 1102 19 0.964968373804389 1102 20 0.8450489566185553 1102 21 0.9083917017287462 1102 22 0.9579164889871308 1102 23 1.0272310412107544 1102 24 0.9469084447144098 1102 25 1.0827652856468646 1103 1 0.535787237045966 1103 2 0.417651172673871 1103 3 0.4318658408675802 1103 4 0.4937242478042094 1103 5 0.5359113788336529 1103 6 0.6932448613412845 1103 7 0.5513503078124131 1103 8 0.6245535119737653 1103 9 0.6802110201810123 1103 10 0.7154840242653435 1103 11 0.6888102346998224 1103 12 0.8659505418176039 1103 13 0.85195393845175 1103 14 0.9274719272668861 1103 15 0.723677289946373 1103 16 0.8302694331554505 1103 17 0.8892613206320519 1103 18 1.066217935984774 1103 19 1.1217801954308442 1103 20 1.0820118018418796 1103 21 0.7326012931230181 1103 22 0.9999152552225041 1103 23 0.9799507267358578 1103 24 1.1354981481831317 1103 25 1.1378168538562576 1104 1 0.572563808771104 1104 2 0.6085284465907534 1104 3 0.645874410889359 1104 4 0.5589276083883163 1104 5 0.6376706286462408 1104 6 0.7113341723239586 1104 7 0.6945641546964652 1104 8 0.7324847743663625 1104 9 0.9139960687755249 1104 10 0.9569865282908552 1104 11 0.9387008915130295 1104 12 0.8999019340025569 1104 13 0.9513994598169191 1104 14 1.0704176282831384 1104 15 1.1285227678404155 1104 16 1.0638111765250002 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2216 17 -1.942851743163601 2216 18 -1.776698699630849 2216 19 -2.048825716395882 2216 20 -2.388161879438499 2216 21 -2.2267197191556605 2216 22 -2.5305427513442424 2216 23 -1.5486924375775004 2216 24 -1.8848298385255617 2216 25 -1.7142130077510558 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53262.0 3 0.0 4 846.0 5 7331.0 6 8371.0 7 6614.0 8 5355.0 9 4700.0 10 4138.0 11 3767.0 12 3525.0 13 3325.0 14 3133.0 15 3262.0 16 3384.0 17 3644.0 18 3837.0 19 4533.0 20 5776.0 21 7519.0 22 9597.0 23 11714.0 24 13965.0 25 16092.0 26 18140.0 27 20140.0 28 22774.0 29 27480.0 30 34614.0 31 44462.0 32 56310.0 33 72585.0 34 93929.0 35 121729.0 36 167224.0 37 259521.0 38 809992.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.76457176638872 19.138892219158375 13.411368437589141 27.68516757686377 2 14.081636446819298 20.620101560168255 39.220669776606876 26.07759221640557 3 16.928415410592844 24.571953795072783 30.216256409461995 28.283374384872378 4 11.614385840400788 17.441749350589646 37.26703743361913 33.67682737539043 5 12.990458649410275 37.82272405223428 35.906237096593316 13.280580201762124 6 32.105226439421926 37.30060747313398 17.726949740706385 12.867216346737706 7 27.667690368506832 31.612239995660673 22.380481303450537 18.339588332381954 8 25.188183419602904 36.55700948681101 20.44431284824978 17.8104942453363 9 26.164901402309265 14.878076124474514 19.82472554922445 39.13229692399177 10 14.57618444519114 28.97986507792913 34.56046938406266 21.88348109281708 11 34.39362923915244 22.345275325437186 24.425191792083748 18.835903643326628 12 22.90422269573723 24.767784269960018 31.657720262804272 20.67027277149848 13 28.83713868757882 21.699194725280176 25.64129178582605 23.82237480131496 14 21.174780424526354 21.487463748357833 27.493513951201777 29.844241875914037 15 23.166539974220843 30.08833699639753 23.317693013187032 23.4274300161946 16 22.653396602158356 27.932415012642746 26.84812383281826 22.566064552380638 17 22.050832820267015 28.15200054543426 27.340357176799213 22.456809457499517 18 21.986660855663352 27.490193931907985 28.680554551482118 21.842590660946545 19 23.509069079924092 26.88558113114987 27.292547217309355 22.312802571616686 20 23.57148611300457 27.092189572050017 27.581322476565823 21.75500183837958 21 23.274709579275292 26.488972610060895 27.045054004803298 23.191263805860515 22 23.214949622634496 27.36762257666076 27.019328880131198 22.398098920573545 23 22.77043669542856 27.057818123609024 27.229821490351597 22.941923690610817 24 23.08578480731595 26.749802487878178 27.61596414315885 22.54844856164702 25 23.00672549408421 26.94586968735541 27.40530396919823 22.64210084936215 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 160.0 1 160.0 2 274.5 3 389.0 4 389.0 5 389.0 6 1011.5 7 1634.0 8 1634.0 9 1634.0 10 2404.0 11 3174.0 12 3174.0 13 3174.0 14 5652.0 15 8130.0 16 8130.0 17 8130.0 18 15038.0 19 21946.0 20 21946.0 21 21946.0 22 34487.5 23 47029.0 24 47029.0 25 47029.0 26 66912.5 27 86796.0 28 86796.0 29 86796.0 30 110628.0 31 134460.0 32 134460.0 33 134460.0 34 154662.5 35 174865.0 36 174865.0 37 174865.0 38 192447.0 39 210029.0 40 210029.0 41 210029.0 42 223035.0 43 236041.0 44 236041.0 45 236041.0 46 242673.0 47 249305.0 48 249305.0 49 249305.0 50 244200.0 51 239095.0 52 239095.0 53 239095.0 54 217787.5 55 196480.0 56 196480.0 57 196480.0 58 170593.0 59 144706.0 60 144706.0 61 144706.0 62 119041.5 63 93377.0 64 93377.0 65 93377.0 66 72247.5 67 51118.0 68 51118.0 69 51118.0 70 37431.0 71 23744.0 72 23744.0 73 23744.0 74 16552.5 75 9361.0 76 9361.0 77 9361.0 78 6366.5 79 3372.0 80 3372.0 81 3372.0 82 2202.0 83 1032.0 84 1032.0 85 1032.0 86 656.0 87 280.0 88 280.0 89 280.0 90 170.0 91 60.0 92 60.0 93 60.0 94 33.0 95 6.0 96 6.0 97 6.0 98 3.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0763197166152877 2 2.0654862412797752E-4 3 0.0 4 0.031137205087292612 5 0.07946958313323936 6 0.07874666294879143 7 0.04156791060575548 8 0.07110436385605627 9 0.051482244563898404 10 0.11050351390846798 11 0.0060931844117753375 12 0.012599466071806628 13 0.005576812851455393 14 0.006506281660031292 15 0.007332476156543202 16 0.015594421121662302 17 0.026748046824573088 18 0.001755663305087809 19 0.003356415142079635 20 0.005266989915263427 21 1.5491146809598315E-4 22 3.098229361919663E-4 23 0.0 24 0.0 25 0.004027698170495562 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1936590.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.13554150819161 #Duplication Level Percentage of deduplicated Percentage of total 1 82.56469783763318 60.38413885815519 2 11.843067264293502 17.32298274984085 3 3.1051570064256455 6.812920173986843 4 1.0749487848958956 3.144678459077356 5 0.4877791515476637 1.783699619242232 6 0.269361447053362 1.1819937175006627 7 0.15629499410987746 0.8001503320471852 8 0.09985975527008709 0.5842637821242648 9 0.07137854642678818 0.4698277780491673 >10 0.2999974867167507 3.671502703137233 >50 0.015018814623872997 0.745981403018661 >100 0.010531600174063788 1.525163248086911 >500 0.0012715405528571668 0.6423874604339739 >1k 6.357702764285667E-4 0.930309715299529 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3665 0.1892501768572594 No Hit TATCAACGCAGAGTACTTTTTTTTT 2614 0.13497952586763332 No Hit GGTATCAACGCAGAGTACTTTTTTT 2327 0.12015966208645092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.163715603199438E-5 7 0.0 0.0 0.0 0.0 5.163715603199438E-5 8 0.0 0.0 0.0 0.0 5.163715603199438E-5 9 0.0 0.0 0.0 5.163715603199438E-5 5.163715603199438E-5 10 0.0 0.0 0.0 5.163715603199438E-5 5.163715603199438E-5 11 0.0 0.0 0.0 5.163715603199438E-5 5.163715603199438E-5 12 0.0 0.0 0.0 5.163715603199438E-5 5.163715603199438E-5 13 5.163715603199438E-5 0.0 0.0 5.163715603199438E-5 1.0327431206398876E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 40 0.005217034 14.275677 5 TAGGCTC 90 5.290476E-7 12.68949 5 TAGGACC 440 0.0 12.328993 4 CCGGAAC 75 2.0566628E-4 11.410494 3 GTATTAC 225 0.0 10.988169 1 GTAGGAC 1090 0.0 10.642817 3 AGGACGT 1020 0.0 10.543474 5 GGTATCA 915 0.0 10.496264 1 GTATCAA 1890 0.0 10.313986 1 AGGACCT 860 0.0 10.291767 5 GGACGTG 1055 0.0 10.28177 6 TAAGACA 315 0.0 10.272445 4 TGTAGGA 1185 0.0 10.190544 2 CTAGTAT 140 9.5602445E-8 10.187942 3 CTATACA 215 1.8189894E-12 10.181103 4 GATGCGA 75 0.0026133033 10.149489 6 GACGTGA 665 0.0 10.147206 7 ACGAAAT 75 0.0026693982 10.12354 12 TATGGCG 1060 0.0 10.117571 16 TTAGGAC 705 0.0 10.115687 3 >>END_MODULE