FastQCFastQC Report
Thu 26 May 2016
SRR1512952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788978
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29910.1671904293960015No Hit
GTCCTACAGTGGACATTTCTAAATT23480.13124812043524292No Hit
GTCCTAAAGTGTGTATTTCTCATTT22950.12828553509322083No Hit
CTGTAGGACGTGGAATATGGCAAGA21300.11906239204730298No Hit
CTTTAGGACGTGAAATATGGCGAGG18210.10178996052494775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG508.7325236E-515.1964435
CGTGCGC1052.0076186E-812.66051610
GGCGAGG6950.012.43176519
TAGGACC4300.012.1486594
GGTATCA7400.011.7147571
TGGCGAG12350.011.31010718
AAGGCGA600.00586691111.085717
ACTGCGT600.005899983511.0773318
TCCAACG1553.110472E-1011.03461418
TATGGCG13550.010.72922816
AGGACGT13700.010.6763325
GTCCTAA7200.010.5847621
GTATCAA15950.010.5714891
CCTATAC1355.7045327E-810.553383
ATGGCGA14050.010.55029517
CCCTATA1451.4479156E-810.4805982
CGTGAAA8350.010.4619839
GTCCTAT2100.010.4335521
ACGTGAA8700.010.3679468
TTAGGAC10300.010.3279683
Produced by FastQC (version 0.11.5)