##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512952_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1788978
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.08618048964269	31.0	30.0	34.0	23.0	34.0
2	29.10747477051143	31.0	30.0	34.0	22.0	34.0
3	29.28432546403589	31.0	30.0	34.0	23.0	34.0
4	32.74955645066625	35.0	33.0	37.0	25.0	37.0
5	32.856381129337535	35.0	35.0	37.0	26.0	37.0
6	32.954575182031306	37.0	35.0	37.0	26.0	37.0
7	32.75765325230383	36.0	35.0	37.0	25.0	37.0
8	32.75578291068979	37.0	35.0	37.0	25.0	37.0
9	34.10641606548543	39.0	35.0	39.0	25.0	39.0
10	33.98205064567591	38.0	34.0	39.0	25.0	39.0
11	34.058078411249326	39.0	35.0	39.0	25.0	39.0
12	33.961268947969174	39.0	35.0	39.0	24.0	39.0
13	33.95688543961972	39.0	35.0	39.0	23.0	39.0
14	34.94830959352211	39.0	35.0	41.0	23.0	41.0
15	34.899364329801706	39.0	35.0	41.0	22.0	41.0
16	34.78156634681925	39.0	34.0	41.0	21.0	41.0
17	34.71515356812661	39.0	34.0	41.0	19.0	41.0
18	34.41570718030071	39.0	34.0	41.0	17.0	41.0
19	34.659537456581354	39.0	34.0	41.0	18.0	41.0
20	34.75631282218116	39.0	34.0	41.0	19.0	41.0
21	34.68989780757505	39.0	34.0	41.0	18.0	41.0
22	34.59185691495368	39.0	34.0	41.0	18.0	41.0
23	34.323728408063154	39.0	34.0	41.0	16.0	41.0
24	34.33973643052067	39.0	34.0	41.0	16.0	41.0
25	34.25386673284971	39.0	34.0	41.0	15.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5405132475160208
1101	2	0.7888974054845121
1101	3	0.9133633340139973
1101	4	0.7550836996884343
1101	5	0.6583049638482876
1101	6	0.7833468122663447
1101	7	0.7697207238862376
1101	8	0.6699275545523733
1101	9	0.5807591079910353
1101	10	0.72335332370087
1101	11	0.9069556103673193
1101	12	1.1241806475200917
1101	13	1.0043559976200243
1101	14	0.7654387466279218
1101	15	0.6852374213249348
1101	16	1.1881387687273701
1101	17	1.1026676822019184
1101	18	1.3958565280272879
1101	19	0.8417825006158637
1101	20	1.0387988865558384
1101	21	1.1522817933016185
1101	22	1.088106656433446
1101	23	1.3669654316232211
1101	24	1.0535689161706898
1101	25	1.3093809067321445
1102	1	0.892871943513434
1102	2	0.9490701147131659
1102	3	1.0554638059739148
1102	4	1.2660044010552198
1102	5	0.9646224006327344
1102	6	0.9411328050644698
1102	7	1.1308989925195547
1102	8	1.1060501187799971
1102	9	1.0477886675292112
1102	10	1.3106182656439316
1102	11	1.2397080782380314
1102	12	1.2478374297838357
1102	13	1.1852387946890985
1102	14	1.2006065704496507
1102	15	1.4482900712073317
1102	16	1.2228246195660688
1102	17	1.1658926537104435
1102	18	1.4755481471453535
1102	19	1.4916140280982262
1102	20	1.390533919310947
1102	21	1.0964476484254817
1102	22	1.5425706263554915
1102	23	1.8247878159925008
1102	24	1.6724829507739045
1102	25	1.5946421050807373
1103	1	0.48540314730856693
1103	2	0.6438915124969
1103	3	0.47175404175914437
1103	4	0.7076158178041752
1103	5	0.1645424645815723
1103	6	0.59349901667035
1103	7	0.3661246681881707
1103	8	0.4558646462429934
1103	9	0.733677603439304
1103	10	0.722934948245566
1103	11	0.7481222619237613
1103	12	0.8067035395104298
1103	13	0.8630173561536552
1103	14	1.0291147443027242
1103	15	1.0813747146037258
1103	16	1.1124645416153598
1103	17	0.8730811512783987
1103	18	1.2828079602099365
1103	19	1.141012679112059
1103	20	1.0960035305500568
1103	21	0.9907651356122074
1103	22	1.0487868298632748
1103	23	1.3572732002341326
1103	24	1.2839396677532662
1103	25	1.1464182005442396
1104	1	0.6996589766876191
1104	2	0.575663541682065
1104	3	0.47901716742881817
1104	4	0.7808006741743938
1104	5	0.6414545519329735
1104	6	0.7369068436002948
1104	7	0.7950725736935524
1104	8	0.7817180061082922
1104	9	0.8434638170216289
1104	10	0.9233278254331765
1104	11	0.8979436813839428
1104	12	0.7836360360663761
1104	13	0.7182012941096687
1104	14	1.0538073306591684
1104	15	1.1183715001540122
1104	16	0.9703578014755578
1104	17	1.038273252916241
1104	18	1.1321497633631523
1104	19	1.0278614979293437
1104	20	1.2089404150849816
1104	21	1.2715345626237848
1104	22	0.9731018459355241
1104	23	1.364918998489145
1104	24	1.2324676538750055
1104	25	0.9728455692875428
1105	1	0.5757167011036195
1105	2	0.6284787408168455
1105	3	0.6688535933195823
1105	4	0.7057110871965335
1105	5	0.5266197458345729
1105	6	0.3103038721534972
1105	7	0.696217642297988
1105	8	0.7050901147789119
1105	9	0.5771545014711208
1105	10	0.9599792411317054
1105	11	0.7223559459533533
1105	12	0.8548647856365079
1105	13	0.7453858956158328
1105	14	0.9090671702748025
1105	15	0.9411238513449334
1105	16	1.0205205283087864
1105	17	0.7534180623538944
1105	18	0.9016896924910114
1105	19	0.9391003649718002
1105	20	1.0441220561090887
1105	21	0.9516646942506028
1105	22	0.5486771214248236
1105	23	0.8822295949216112
1105	24	0.9739610027583439
1105	25	1.0248850124776823
1106	1	0.599079345160483
1106	2	0.7578280686344279
1106	3	0.7010848547291637
1106	4	0.7082246791570199
1106	5	0.7374820166226641
1106	6	0.7462437330785292
1106	7	0.538325943994721
1106	8	0.7190364317607489
1106	9	0.9508719499451033
1106	10	0.9295279741081615
1106	11	0.9511838919398627
1106	12	0.880373725174934
1106	13	0.9063886647724786
1106	14	1.14982876798463
1106	15	1.251192005247134
1106	16	1.1230470104844557
1106	17	1.0949741751029691
1106	18	1.31275069157725
1106	19	0.7518147456741033
1106	20	0.7795128137750353
1106	21	0.7797689467276143
1106	22	0.942598862390227
1106	23	1.2234266231048991
1106	24	0.9877405930187209
1106	25	1.1804400482219037
1107	1	0.6761885907342631
1107	2	0.5970848761733052
1107	3	0.6652083423580741
1107	4	0.7896851377177043
1107	5	0.7220311112434175
1107	6	0.6762173880675348
1107	7	0.7509878965358254
1107	8	0.7282098383510913
1107	9	0.8532326842163442
1107	10	0.7804005591488234
1107	11	0.7571720242251132
1107	12	0.9312538359376106
1107	13	0.8532914563667973
1107	14	1.0565888100887975
1107	15	1.2631526701421834
1107	16	1.1433235982124472
1107	17	1.2089906080901542
1107	18	1.4872143717450257
1107	19	1.3906137301501857
1107	20	1.2594789430420192
1107	21	1.1808929207873007
1107	22	1.162133393991283
1107	23	1.2649332228142356
1107	24	1.2051626893359995
1107	25	1.2447869650681582
1108	1	0.5989243920238749
1108	2	0.5379570174561863
1108	3	0.4267567156368699
1108	4	0.6936208426762818
1108	5	0.7064799664734664
1108	6	0.7747536202805918
1108	7	0.8289365785476832
1108	8	0.820251621935931
1108	9	0.9607725614140179
1108	10	0.9223017941797593
1108	11	0.8831480421411086
1108	12	0.939841461150678
1108	13	0.9505046173965539
1108	14	1.1745353503144074
1108	15	0.9366326681287092
1108	16	0.9361904136526533
1108	17	0.9565717220756227
1108	18	1.146924016593438
1108	19	1.1896959204572681
1108	20	1.130529346082767
1108	21	1.0618680849497864
1108	22	0.9365929384126161
1108	23	1.1764211917988021
1108	24	1.1283744705421697
1108	25	1.3192601794155578
1109	1	0.18312291581924
1109	2	0.19042199688882988
1109	3	0.3187615749189092
1109	4	0.5273373425116006
1109	5	0.42667331664510755
1109	6	0.3385652335041627
1109	7	0.5621090370100319
1109	8	0.5386978229564363
1109	9	0.7837055896156713
1109	10	0.8175849669885977
1109	11	0.37275574571489045
1109	12	0.6878678157981781
1109	13	0.6516470091764148
1109	14	0.5917300909975296
1109	15	0.7588486669193912
1109	16	0.7874975344930064
1109	17	0.6788673574312512
1109	18	-0.7661645106356616
1109	19	0.5283975891711634
1109	20	0.7498633121038267
1109	21	0.7921831874662217
1109	22	0.9608362909368111
1109	23	0.5528508708599418
1109	24	0.8807515544323579
1109	25	0.9594227861690996
1110	1	0.2562102280919518
1110	2	0.26878118903201553
1110	3	0.3606885605781436
1110	4	0.20840778715960084
1110	5	0.34281112669305713
1110	6	0.2804256233264084
1110	7	0.39883965460796844
1110	8	0.3675006967884613
1110	9	0.6476112436869101
1110	10	0.6915958046267576
1110	11	0.5106544552897958
1110	12	0.11742989213384902
1110	13	0.3223789108735886
1110	14	0.38733566233452166
1110	15	0.5806808942238817
1110	16	0.7364899549204438
1110	17	0.2947000320286506
1110	18	0.8150512793020397
1110	19	0.6487985787942634
1110	20	0.6584900382036665
1110	21	0.5963495864046777
1110	22	0.8050575704236493
1110	23	0.6260472668147798
1110	24	0.8356581533974108
1110	25	0.8072273898989621
1111	1	0.08689638661047994
1111	2	0.33703359488650264
1111	3	0.3479205446415712
1111	4	0.2888611210502532
1111	5	0.25696880108723974
1111	6	0.4184148035441382
1111	7	0.21949766007953997
1111	8	0.06194507043541364
1111	9	0.2928532869976408
1111	10	0.3493910076493236
1111	11	0.19846147922778812
1111	12	0.34121883450598745
1111	13	0.28483998570523283
1111	14	0.2782875901020532
1111	15	0.3668212149637071
1111	16	0.32518479638305564
1111	17	0.41335368124565974
1111	18	0.6331180116574515
1111	19	0.21098734948532893
1111	20	0.16143226864066662
1111	21	0.437639396612461
1111	22	0.20753527303715202
1111	23	0.41195208678157513
1111	24	0.6047244132220442
1111	25	0.11398524260087584
1112	1	-0.3444158000812898
1112	2	-0.04459515040207762
1112	3	0.016766184910864013
1112	4	0.18776036458712753
1112	5	0.08811850820527667
1112	6	0.04371896809774256
1112	7	-0.2003551415945779
1112	8	-0.0371139644989853
1112	9	0.20952215073140934
1112	10	0.23994630731117894
1112	11	0.10548987102119867
1112	12	0.24711121337134045
1112	13	0.020452427205093215
1112	14	0.12112750352209645
1112	15	0.4053947342567952
1112	16	0.2951247828491219
1112	17	0.3863571546795015
1112	18	0.3320880266026762
1112	19	0.2940207185403878
1112	20	0.13868021722619517
1112	21	0.14534236554800373
1112	22	9.820163892158007E-4
1112	23	0.28641167289548974
1112	24	-0.027675244138876565
1112	25	0.1507897351636771
1113	1	-0.4628619220138006
1113	2	-0.3941316319584729
1113	3	-0.46078655654800116
1113	4	-0.2187954751205794
1113	5	-0.4033957790108502
1113	6	-0.37298940722993024
1113	7	-0.4467885749525138
1113	8	-0.20407125863913222
1113	9	-0.44309688654890067
1113	10	-0.5055207801749262
1113	11	-0.38511710998022153
1113	12	-0.20839650101688534
1113	13	-0.41559467349065926
1113	14	-0.16878235244399065
1113	15	-0.6970939206065125
1113	16	-0.6316706131241432
1113	17	-0.7264443951305424
1113	18	-0.5537635576027284
1113	19	-0.6915187553743678
1113	20	-0.8278180150121983
1113	21	-0.6244448707637531
1113	22	-0.451132631768715
1113	23	-0.9148659452847596
1113	24	-0.31241893965574974
1113	25	-0.28183192271560387
1114	1	-1.635765051130523
1114	2	-1.3580567779002557
1114	3	-1.2185500198082835
1114	4	-1.3422874658952502
1114	5	-1.3074041190573382
1114	6	-1.8630176966304681
1114	7	-1.9498955853430076
1114	8	-1.5255712546409015
1114	9	-1.5878968551821515
1114	10	-1.8379365845785003
1114	11	-2.393691246291951
1114	12	-1.857010521591512
1114	13	-1.8100843924977497
1114	14	-2.093411619940177
1114	15	-2.128863766071113
1114	16	-2.757300753265916
1114	17	-4.625519824159795
1114	18	-3.726643084274393
1114	19	-2.706400806346881
1114	20	-1.8391603441014794
1114	21	-2.7713885346938234
1114	22	-2.9351174054831723
1114	23	-2.812846117683211
1114	24	-2.3915303372635677
1114	25	-2.243130232599974
1115	1	-1.1092663306583965
1115	2	-0.6954145978431043
1115	3	-0.8059967767318668
1115	4	-0.7472479789345776
1115	5	-0.6106516952599179
1115	6	-0.774341968273653
1115	7	-1.8233499082625997
1115	8	-1.2192643521782998
1115	9	-0.9677787278138084
1115	10	-0.8407515988348848
1115	11	-1.6374426293149398
1115	12	-1.1990105342967965
1115	13	-0.9509700989485381
1115	14	-0.9580770633704319
1115	15	-2.291132463747843
1115	16	-1.0605893569447318
1115	17	-1.054587023460158
1115	18	-1.1007083195497671
1115	19	-1.1917788785107675
1115	20	-1.0391110581913239
1115	21	-1.7301596267870067
1115	22	-1.6643609435233202
1115	23	-1.114117757691723
1115	24	-1.0540420234769599
1115	25	-1.3583094459100309
1116	1	-1.1054683547780186
1116	2	-1.789312102547008
1116	3	-1.441907342300869
1116	4	-0.9832966479527592
1116	5	-1.5049703237642618
1116	6	-1.2902883306759705
1116	7	-1.0059483495542203
1116	8	-1.6851745508690712
1116	9	-1.674614979576134
1116	10	-2.5835309110954334
1116	11	-1.329770157236993
1116	12	-1.3188437095230796
1116	13	-1.8877071887655674
1116	14	-1.6591504442581737
1116	15	-1.3475405162009508
1116	16	-1.1474307450023247
1116	17	-1.52891838788031
1116	18	-1.4941998996943155
1116	19	-1.8495797579703037
1116	20	-2.1105404157875327
1116	21	-2.295239370391535
1116	22	-1.4922536473634835
1116	23	-1.4854595886237547
1116	24	-2.062808840913114
1116	25	-1.614028815508398
1201	1	0.2788950277888169
1201	2	0.26825059822502695
1201	3	0.632569494775435
1201	4	0.6470695237746327
1201	5	0.6800755046698086
1201	6	0.6532111152680926
1201	7	0.5212494816195345
1201	8	0.10838381641728034
1201	9	0.3692697663719642
1201	10	0.6697326802026993
1201	11	0.2782509580886767
1201	12	-0.28984451293674596
1201	13	0.0012804449142578278
1201	14	0.4966983941775567
1201	15	0.16818816176038354
1201	16	0.719360144781561
1201	17	0.8130869951214379
1201	18	1.2814837506423515
1201	19	0.7467029238427259
1201	20	0.7071299971045875
1201	21	0.5556464029364321
1201	22	0.8210909624079505
1201	23	1.1135449336706529
1201	24	0.8709146103019734
1201	25	0.6952685165592598
1202	1	0.6875000376623355
1202	2	0.6887180381662823
1202	3	0.4267773263021546
1202	4	0.9255646507577637
1202	5	0.7732357877997345
1202	6	0.8425555890113401
1202	7	0.8302241830187711
1202	8	0.8859365630593743
1202	9	1.1811760208080102
1202	10	1.2747154404887482
1202	11	0.9883823516788226
1202	12	0.9803376710367004
1202	13	0.9669780351345167
1202	14	1.2670119078686284
1202	15	1.0659038849973754
1202	16	1.1898513674592834
1202	17	1.4128186523356234
1202	18	1.6752365752089204
1202	19	1.3995419725280556
1202	20	1.2762850979573344
1202	21	1.349616081218386
1202	22	1.4839520001851554
1202	23	1.471212914155906
1202	24	1.5434241784703033
1202	25	1.3057447044284132
1203	1	0.46091325646262504
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2211	15	0.11364168335861535
2211	16	0.09401951851022261
2211	17	0.06654148810334704
2211	18	0.0343388195979486
2211	19	-0.06287341756813447
2211	20	-0.28659342003339816
2211	21	-0.2923504310723146
2211	22	-0.3706970913716674
2211	23	0.35721220165144985
2211	24	0.14335292802498856
2211	25	0.16965635387622058
2212	1	-0.2787195947946728
2212	2	-0.31185027795196874
2212	3	-0.26629280602244165
2212	4	-0.4236524443623395
2212	5	-0.26843992999057065
2212	6	-0.4588949960092208
2212	7	-0.38895644189724976
2212	8	-0.38460533196320057
2212	9	-0.2580839819901257
2212	10	-0.41879103470084544
2212	11	-0.4496037566251303
2212	12	-0.23521469684428098
2212	13	-0.2859547493008989
2212	14	-0.39604413177774944
2212	15	-0.4598570295873472
2212	16	-0.2291164883644754
2212	17	-0.16585270693725107
2212	18	-2.9634535247696334
2212	19	-1.2102972379595869
2212	20	-0.5416191390767011
2212	21	-0.6869061314038731
2212	22	-0.42485360031643893
2212	23	-0.42786734056758746
2212	24	-0.8183438500772198
2212	25	-0.5629249082137164
2213	1	-0.40139212024758564
2213	2	-0.2647441642399855
2213	3	-0.6225241443095975
2213	4	-0.7484263143787686
2213	5	-0.5388286266962083
2213	6	-0.6487177664561088
2213	7	-0.40242860409525605
2213	8	-0.8324250092857284
2213	9	-0.6530550212329516
2213	10	-0.6549327396507962
2213	11	-0.6952576122738279
2213	12	-0.8168624550906571
2213	13	-0.5936873698321676
2213	14	-0.6583792820202063
2213	15	-0.7296517245680931
2213	16	-1.2434484299822302
2213	17	-0.8959564732001155
2213	18	-0.8734692442383576
2213	19	-0.5791886822405132
2213	20	-1.2770897363086249
2213	21	-0.9547568668935114
2213	22	-0.8514120774381766
2213	23	-1.218374863002893
2213	24	-0.8847834506999064
2213	25	-1.1720052653509114
2214	1	-1.8883763289247781
2214	2	-4.031275119886015
2214	3	-2.0440177299805384
2214	4	-1.8270497286298273
2214	5	-1.7685954068516914
2214	6	-1.8362139081253481
2214	7	-2.021798457848689
2214	8	-1.6991934981067338
2214	9	-1.834656500165302
2214	10	-1.927458557966915
2214	11	-1.6602159023016299
2214	12	-2.3220805182786144
2214	13	-2.0061789615044354
2214	14	-3.28790232900484
2214	15	-3.5762820934811828
2214	16	-2.876994540951486
2214	17	-3.3158552599342563
2214	18	-2.5941927637560127
2214	19	-4.44293170641183
2214	20	-2.9237786385160263
2214	21	-2.941633408975818
2214	22	-2.9946075819745026
2214	23	-2.922960926612184
2214	24	-3.070180793768344
2214	25	-2.932867824775869
2215	1	-1.110562702962035
2215	2	-2.7069818871440425
2215	3	-1.4320881304047646
2215	4	-1.1774328645614105
2215	5	-1.1362883461796542
2215	6	-1.4837264160981896
2215	7	-2.1613995419519156
2215	8	-1.9130946125794601
2215	9	-1.7998916067663018
2215	10	-3.31621602605696
2215	11	-2.2432472654051914
2215	12	-2.5111765826213706
2215	13	-1.8909139250508886
2215	14	-3.181560649808219
2215	15	-2.9554338713144226
2215	16	-2.594812915107788
2215	17	-2.8287809655854836
2215	18	-1.7027570304205142
2215	19	-2.3000344831581288
2215	20	-2.562119151632068
2215	21	-2.138578602512048
2215	22	-1.8130063585368248
2215	23	-2.393087822423155
2215	24	-2.199391044725928
2215	25	-2.146599844036494
2216	1	-2.1193516139048825
2216	2	-2.1248500861524313
2216	3	-2.5394018765555337
2216	4	-3.1357292370936136
2216	5	-2.3385664384305436
2216	6	-2.446660246053998
2216	7	-2.4418895482413774
2216	8	-2.851518326302738
2216	9	-3.147022141697324
2216	10	-2.6732087645868603
2216	11	-3.3709699082862556
2216	12	-3.7824104607691638
2216	13	-4.303802013462761
2216	14	-3.59339872474267
2216	15	-3.396226343563189
2216	16	-2.697054659167513
2216	17	-2.65621200612393
2216	18	-2.7335500744385826
2216	19	-2.868619435823799
2216	20	-2.9643302651936168
2216	21	-3.3817316651534632
2216	22	-3.793319933156802
2216	23	-3.826530608736629
2216	24	-3.8497700460900113
2216	25	-3.905661570616413
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85565.0
3	3.0
4	679.0
5	4038.0
6	4838.0
7	4427.0
8	4018.0
9	3842.0
10	3687.0
11	3445.0
12	3325.0
13	3315.0
14	3306.0
15	3302.0
16	3528.0
17	3621.0
18	3952.0
19	5033.0
20	6291.0
21	7986.0
22	9890.0
23	12101.0
24	14114.0
25	16142.0
26	17967.0
27	20080.0
28	22516.0
29	26762.0
30	32979.0
31	41495.0
32	52504.0
33	67594.0
34	87721.0
35	116426.0
36	161150.0
37	253658.0
38	677678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02743230834042	18.79009064447385	12.496080337514494	25.686396709671232
2	14.760211981767824	20.54866527202034	41.13865546923362	23.552467276978213
3	18.552433440154154	24.923602890480794	29.86574798152266	26.65821568784239
4	12.18806406806729	17.017045899343973	38.703596839093954	32.09129319349478
5	13.068676523959416	37.880657826381395	35.99457123796241	13.056094411696778
6	31.311690700473704	37.440688872849584	18.578310814626136	12.669309612050577
7	27.416084250355993	31.715482294376436	22.858393587634158	18.010039867633417
8	24.740363030491093	36.756767334524234	21.108324876786007	17.394544758198656
9	26.373023043618275	15.172245679321572	20.352531130353885	38.10220014670627
10	14.654674533452383	28.92877569652978	35.06513691863602	21.35141285138182
11	33.9557808790823	22.535582940762747	25.10843446242336	18.400201717731594
12	22.93269969948983	24.77031239080299	32.13720036340765	20.159787546299533
13	28.656290341788072	22.192994275484548	25.89047552223135	23.260239860496036
14	20.90403535492471	21.99282971352402	27.995596290058888	29.107538641492386
15	22.91889504245213	30.678814751032295	23.60724300175847	22.7950472047571
16	22.291872949172177	28.13530305986726	27.57615283849387	21.996671152466696
17	21.77744852745901	28.419943250912038	27.792572299176722	22.010035922452232
18	21.5595241958859	27.74488814977377	29.41080687272609	21.284780781614238
19	23.349590532171195	27.08045272655198	27.602610172201814	21.967346569075016
20	23.381907963153058	27.49325165996865	27.971282374700042	21.153558002178254
21	23.000607206637003	26.90557209935824	27.208728120153108	22.885092573851647
22	23.05547992344975	27.91512381829882	27.311533023337986	21.717863234913445
23	22.47673158025096	27.64982111537457	27.55220193862963	22.321245365744836
24	22.883488507183394	26.99342908211215	28.209549271820638	21.91353313888381
25	22.763258533007118	27.270958478886094	27.862376047197113	22.103406940909682
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	256.0
1	256.0
2	401.5
3	547.0
4	547.0
5	547.0
6	1045.0
7	1543.0
8	1543.0
9	1543.0
10	2452.0
11	3361.0
12	3361.0
13	3361.0
14	5829.0
15	8297.0
16	8297.0
17	8297.0
18	14916.5
19	21536.0
20	21536.0
21	21536.0
22	34159.0
23	46782.0
24	46782.0
25	46782.0
26	66418.0
27	86054.0
28	86054.0
29	86054.0
30	110388.0
31	134722.0
32	134722.0
33	134722.0
34	153456.0
35	172190.0
36	172190.0
37	172190.0
38	187766.0
39	203342.0
40	203342.0
41	203342.0
42	212893.5
43	222445.0
44	222445.0
45	222445.0
46	226163.5
47	229882.0
48	229882.0
49	229882.0
50	221614.5
51	213347.0
52	213347.0
53	213347.0
54	192438.5
55	171530.0
56	171530.0
57	171530.0
58	146851.5
59	122173.0
60	122173.0
61	122173.0
62	99810.0
63	77447.0
64	77447.0
65	77447.0
66	59796.5
67	42146.0
68	42146.0
69	42146.0
70	30809.5
71	19473.0
72	19473.0
73	19473.0
74	13576.0
75	7679.0
76	7679.0
77	7679.0
78	5278.0
79	2877.0
80	2877.0
81	2877.0
82	1904.5
83	932.0
84	932.0
85	932.0
86	611.5
87	291.0
88	291.0
89	291.0
90	189.0
91	87.0
92	87.0
93	87.0
94	55.5
95	24.0
96	24.0
97	24.0
98	19.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35210047300749364
2	0.039743361852409585
3	0.034936147901203925
4	0.03521563708441356
5	0.04052593156539656
6	0.017719614215490634
7	0.017272431522355223
8	0.023644784899534817
9	0.020682199557512725
10	0.02017911902773539
11	0.019005264458254935
12	0.019731936334599978
13	0.019843732007883832
14	0.027166348607976173
15	0.02755763346446966
16	0.02073809739415465
17	0.022526828166696294
18	0.10374638480741519
19	0.024427354612521785
20	0.02180015629035125
21	0.02571300485528609
22	0.01950834498803227
23	0.022079645473560883
24	0.01894936662161301
25	0.022023747636918955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1788978.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.14245151094491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.33651808374889	61.78773751115335
2	11.513390717579211	17.072620260093597
3	2.8936322128485803	6.436229580949021
4	1.026470301947877	3.0442009835838175
5	0.43721686543397814	1.6208165122603024
6	0.234965777978291	1.045256328029213
7	0.13742342362910961	0.7132236666022509
8	0.08146228301309845	0.4831850694615617
9	0.057544641921161	0.3839850741018931
>10	0.25175572434688487	3.185146500144578
>50	0.016170770784009934	0.8258526464822518
>100	0.011485250675776926	1.7740862119791014
>500	0.0012085822111515022	0.5886076102165163
>1k	7.55363881969687E-4	1.0390520449426264
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2991	0.1671904293960015	No Hit
GTCCTACAGTGGACATTTCTAAATT	2348	0.13124812043524292	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2295	0.12828553509322083	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2130	0.11906239204730298	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1821	0.10178996052494775	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.589783664192628E-5	0.0
11	0.0	0.0	0.0	5.589783664192628E-5	0.0
12	0.0	0.0	0.0	5.589783664192628E-5	0.0
13	0.0	0.0	0.0	5.589783664192628E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	50	8.7325236E-5	15.196443	5
CGTGCGC	105	2.0076186E-8	12.660516	10
GGCGAGG	695	0.0	12.431765	19
TAGGACC	430	0.0	12.148659	4
GGTATCA	740	0.0	11.714757	1
TGGCGAG	1235	0.0	11.310107	18
AAGGCGA	60	0.005866911	11.0857	17
ACTGCGT	60	0.0058999835	11.077331	8
TCCAACG	155	3.110472E-10	11.034614	18
TATGGCG	1355	0.0	10.729228	16
AGGACGT	1370	0.0	10.676332	5
GTCCTAA	720	0.0	10.584762	1
GTATCAA	1595	0.0	10.571489	1
CCTATAC	135	5.7045327E-8	10.55338	3
ATGGCGA	1405	0.0	10.550295	17
CCCTATA	145	1.4479156E-8	10.480598	2
CGTGAAA	835	0.0	10.461983	9
GTCCTAT	210	0.0	10.433552	1
ACGTGAA	870	0.0	10.367946	8
TTAGGAC	1030	0.0	10.327968	3
>>END_MODULE