FastQCFastQC Report
Thu 26 May 2016
SRR1512952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788978
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33230.18574851116112104No Hit
TATCAACGCAGAGTACTTTTTTTTT23110.12917990047949163No Hit
GTCCTACAGTGGACATTTCTAAATT22200.12409319734507636No Hit
GTCCTAAAGTGTGTATTTCTCATTT21310.1191182898839449No Hit
CTGTAGGACGTGGAATATGGCAAGA19890.11118079708079137No Hit
GGTATCAACGCAGAGTACTTTTTTT19560.1093361684716078No Hit
CTTTAGGACGTGAAATATGGCGAGG18710.10458485235704407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4850.013.9290854
ACTGTCC3150.011.7668788
GGTATCA7550.011.7102131
AGGACCT9600.011.1998755
CTAGACG600.005807917611.1007614
ATAGGAC1803.6379788E-1211.0926773
GCGCCAC1303.885616E-710.22221813
TGGCGAG12650.010.20147918
GGACGTG12300.010.0539036
TATGTCG856.644856E-410.05041616
GTAGGAC13550.010.0342843
GGACCTG9300.010.0239656
GGCGAGA7800.09.97576319
AGGACGT12500.09.9716555
TAACACT2500.09.8955364
CTAGGAC2900.09.8358723
TGTAGGA14850.09.7950342
GTATCAA15600.09.7504391
TTAGGAC8350.09.6788123
GACGTGA6900.09.6422967
Produced by FastQC (version 0.11.5)