##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512952_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1788978 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.392593983827638 31.0 31.0 34.0 27.0 34.0 2 30.544973163448628 31.0 31.0 34.0 27.0 34.0 3 30.634004442760055 31.0 31.0 34.0 27.0 34.0 4 34.11137643950904 37.0 35.0 37.0 30.0 37.0 5 33.74293367498091 37.0 35.0 37.0 28.0 37.0 6 33.59748918097372 37.0 35.0 37.0 28.0 37.0 7 33.47223107271302 37.0 35.0 37.0 28.0 37.0 8 33.356248092486325 37.0 35.0 37.0 27.0 37.0 9 34.806166425746994 39.0 35.0 39.0 27.0 39.0 10 34.6937888559837 39.0 35.0 39.0 27.0 39.0 11 34.71537212866787 39.0 35.0 39.0 27.0 39.0 12 34.56684430999152 39.0 35.0 39.0 27.0 39.0 13 34.54852211709702 39.0 35.0 39.0 27.0 39.0 14 35.60742781632865 40.0 36.0 41.0 27.0 41.0 15 35.6137174409076 40.0 36.0 41.0 27.0 41.0 16 35.591799340181936 40.0 36.0 41.0 27.0 41.0 17 35.56194151073965 40.0 36.0 41.0 27.0 41.0 18 35.48934251846585 40.0 36.0 41.0 26.0 41.0 19 35.48253080809266 40.0 36.0 41.0 26.0 41.0 20 35.447662296573796 40.0 36.0 41.0 26.0 41.0 21 35.39046315829485 40.0 36.0 41.0 25.0 41.0 22 35.35083047415899 40.0 36.0 41.0 25.0 41.0 23 35.24045125205564 40.0 36.0 41.0 25.0 41.0 24 35.22363606483702 40.0 36.0 41.0 25.0 41.0 25 35.12400431978482 40.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6196490015892309 1101 2 0.6414411646718179 1101 3 0.6308665824642681 1101 4 0.474382910280859 1101 5 0.6507655782059487 1101 6 0.6817230192679489 1101 7 0.6817620858250848 1101 8 0.6280974382837456 1101 9 0.5297238553378705 1101 10 0.9283818391782646 1101 11 0.9343937486162659 1101 12 1.0297293311945097 1101 13 0.9455324492191295 1101 14 1.1261837342647638 1101 15 1.036711924393245 1101 16 1.1575572736762396 1101 17 1.161434206936761 1101 18 0.5511081377392202 1101 19 0.8580672943072614 1101 20 0.9485168373226216 1101 21 1.047999492777059 1101 22 0.9883011424974555 1101 23 0.9811221695976755 1101 24 0.8736468402825324 1101 25 0.9600465275729775 1102 1 0.8560173704231886 1102 2 0.7953056426761798 1102 3 0.7665823793670015 1102 4 0.7405239986795351 1102 5 1.0186752108287322 1102 6 0.8267741155101973 1102 7 0.9329608274088628 1102 8 0.9102675064318078 1102 9 1.150488422867653 1102 10 1.2930108470288317 1102 11 1.1350361998146852 1102 12 1.1880875760656409 1102 13 1.2859368370951358 1102 14 1.3735820868463264 1102 15 1.3334649995079246 1102 16 1.3140894799475404 1102 17 1.3053919410338892 1102 18 1.4190511472467904 1102 19 1.357621551394331 1102 20 1.2436226016721221 1102 21 1.3923474672577427 1102 22 1.275241144317036 1102 23 1.3284355726240946 1102 24 1.3036100234252714 1102 25 1.317734758398899 1103 1 0.5419292944787166 1103 2 0.5059896444943348 1103 3 0.6082588421183708 1103 4 0.601387725998336 1103 5 0.698095334860227 1103 6 0.6823435161987987 1103 7 0.6727674135125312 1103 8 0.6676908488036659 1103 9 0.8245081613252552 1103 10 0.923162412051326 1103 11 0.868609341301493 1103 12 0.819051824274915 1103 13 0.8747259365345386 1103 14 0.9578093455622323 1103 15 0.7121304331740674 1103 16 0.8942823191402454 1103 17 0.9812549107887989 1103 18 1.166214984804192 1103 19 1.1583456779754187 1103 20 1.2058212087994917 1103 21 0.7767084751834261 1103 22 1.0494463553116375 1103 23 1.0754251704882876 1103 24 1.084192354945671 1103 25 1.1304474640513718 1104 1 0.6011772152910027 1104 2 0.6623037421523783 1104 3 0.5567460098310093 1104 4 0.5989156610970667 1104 5 0.6730124224667335 1104 6 0.7619878858094893 1104 7 0.7357119514991624 1104 8 0.717305625067759 1104 9 0.7698165954290346 1104 10 0.7465870765750253 1104 11 0.8173057033607805 1104 12 0.7756669577000892 1104 13 0.9629829904658607 1104 14 1.1536968908451541 1104 15 1.1257663329648722 1104 16 1.0688601037210859 1104 17 0.9574832897877315 1104 18 0.9707917489248103 1104 19 1.0103610869248527 1104 20 1.029304549786282 1104 21 0.9184325427378894 1104 22 1.0777666777770705 1104 23 0.894224599030558 1104 24 0.9525631901923717 1104 25 1.0765799197889052 1105 1 0.4555205016254362 1105 2 0.4536458273484705 1105 3 0.4662036036668269 1105 4 0.30161523197669027 1105 5 0.40870292749307424 1105 6 0.3295098028462178 1105 7 0.44154145963052116 1105 8 0.6272332078889846 1105 9 0.6730746208772231 1105 10 0.714270972848503 1105 11 0.6643170618432634 1105 12 0.5032859127595728 1105 13 0.7048067769921786 1105 14 0.8258222577904277 1105 15 0.8264304478355626 1105 16 0.8170372143105453 1105 17 0.6584476892434807 1105 18 0.8930581624054739 1105 19 0.9172626484046944 1105 20 0.9265232416918039 1105 21 0.8449418154261963 1105 22 0.8850136288294337 1105 23 0.8239226198934873 1105 24 0.8387705335833644 1105 25 0.8079964157543742 1106 1 0.5218822159941965 1106 2 0.5076491101229621 1106 3 0.5228506247828903 1106 4 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0.9083672932439981 1107 17 0.8223507041048848 1107 18 0.9445770664267243 1107 19 1.1049710659127783 1107 20 1.1022306681426457 1107 21 1.0464923947050906 1107 22 1.1333795465489374 1107 23 1.2110417440837367 1107 24 1.1280519330115837 1107 25 1.122775037601258 1108 1 0.5708712461067549 1108 2 0.4344084241970805 1108 3 0.4698720480473142 1108 4 0.5721632514405357 1108 5 0.7499463645554059 1108 6 0.5516544629533158 1108 7 0.5041511704620874 1108 8 0.5631482836972665 1108 9 0.5020627235660697 1108 10 0.7621889187562019 1108 11 0.6095570039287566 1108 12 0.5491284081690893 1108 13 0.8270375795062819 1108 14 0.7439682445300164 1108 15 0.834295870529651 1108 16 0.8144635374194777 1108 17 0.7986235124011998 1108 18 0.9449077108477653 1108 19 0.9350743651519053 1108 20 1.0092578984379657 1108 21 1.0271963317424948 1108 22 0.9171721466280971 1108 23 0.8381972249344898 1108 24 0.9466924810719846 1108 25 0.9011236019629152 1109 1 0.20241948904682872 1109 2 0.28733981061411384 1109 3 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-2.7913486486242576 2216 22 -3.0916764415181603 2216 23 -2.19843036391827 2216 24 -2.379520262404391 2216 25 -2.2555178444193444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48902.0 3 1.0 4 826.0 5 7222.0 6 7870.0 7 6316.0 8 5113.0 9 4589.0 10 3796.0 11 3613.0 12 3202.0 13 3076.0 14 3049.0 15 3055.0 16 2991.0 17 3094.0 18 3556.0 19 4120.0 20 5152.0 21 6687.0 22 8541.0 23 10529.0 24 12258.0 25 13827.0 26 16020.0 27 17748.0 28 20332.0 29 24362.0 30 30219.0 31 39122.0 32 49527.0 33 63976.0 34 83189.0 35 108798.0 36 150045.0 37 233302.0 38 780953.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.38147778720719 19.50456238731091 13.446721240400974 27.667238585080927 2 13.599000993305113 20.805968997924513 40.08944776819378 25.505582240576597 3 16.760966317081596 24.903492385037715 30.41367752985224 27.921863768028448 4 11.349296700789743 17.519799511079004 38.06784505722344 33.06305873090781 5 12.707659501628385 38.09713469324738 36.175686455269506 13.019519349854727 6 31.386545266990158 37.791914718890304 18.138458732799506 12.68308128132003 7 27.158245321096715 31.798592288847523 22.873153336729494 18.17000905332627 8 24.58750840421212 37.039726053449115 20.869910067938477 17.502855474400295 9 26.042952316028295 15.000371888786798 20.166158791478882 38.79051700370603 10 14.311854323090541 29.18193833733055 35.10820456633678 21.39800277324213 11 33.872771881220665 22.43153183218486 25.07584282381825 18.619853462776227 12 22.622869071218613 24.88184644902039 32.129044809461675 20.366239670299322 13 28.514776876653276 22.05586077514596 26.005603518634047 23.423758829566722 14 20.750736364058167 21.81616529437512 27.936609801677832 29.496488539888876 15 22.66129640861664 30.742621718240336 23.6020046230431 22.994077250099924 16 22.16321047273443 28.210548207350396 27.454756778726253 22.171484541188928 17 21.565575324106767 28.462017152624135 27.88797063064604 22.084436892623053 18 21.439152220624766 27.749089413043137 29.34365071024665 21.46810765608545 19 23.214714941333646 27.033534011951275 27.6658114068186 22.085939639896473 20 23.194890250624127 27.486312772025624 27.920938859429345 21.397858117920908 21 22.779095103461305 26.842252951126284 27.42414943056874 22.954502514843668 22 22.830511411594383 27.917069598707638 27.35993336948076 21.89248562021722 23 22.218216210596218 27.54986366517643 27.670714787996275 22.561205336231076 24 22.721855718739974 27.02632452718815 28.239139888808023 22.012679865263856 25 22.553609430541822 27.272122537118953 27.887860268062525 22.286407764276703 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 179.0 1 179.0 2 303.5 3 428.0 4 428.0 5 428.0 6 928.5 7 1429.0 8 1429.0 9 1429.0 10 2273.5 11 3118.0 12 3118.0 13 3118.0 14 5617.5 15 8117.0 16 8117.0 17 8117.0 18 14868.5 19 21620.0 20 21620.0 21 21620.0 22 34229.0 23 46838.0 24 46838.0 25 46838.0 26 66988.0 27 87138.0 28 87138.0 29 87138.0 30 111307.0 31 135476.0 32 135476.0 33 135476.0 34 154309.0 35 173142.0 36 173142.0 37 173142.0 38 187985.5 39 202829.0 40 202829.0 41 202829.0 42 213204.5 43 223580.0 44 223580.0 45 223580.0 46 227359.5 47 231139.0 48 231139.0 49 231139.0 50 222576.5 51 214014.0 52 214014.0 53 214014.0 54 192316.5 55 170619.0 56 170619.0 57 170619.0 58 145921.5 59 121224.0 60 121224.0 61 121224.0 62 98887.5 63 76551.0 64 76551.0 65 76551.0 66 59028.0 67 41505.0 68 41505.0 69 41505.0 70 30350.5 71 19196.0 72 19196.0 73 19196.0 74 13115.0 75 7034.0 76 7034.0 77 7034.0 78 4854.0 79 2674.0 80 2674.0 81 2674.0 82 1761.0 83 848.0 84 848.0 85 848.0 86 530.0 87 212.0 88 212.0 89 212.0 90 135.0 91 58.0 92 58.0 93 58.0 94 33.0 95 8.0 96 8.0 97 8.0 98 5.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0797662128880288 2 5.589783664192628E-5 3 0.0 4 0.030743810153059457 5 0.073561553020775 6 0.07736260591242598 7 0.038290018099719506 8 0.06618303858404072 9 0.04522134984331837 10 0.10620588961965995 11 0.004527724767996029 12 0.011403158674952963 13 0.005701579337476482 14 0.00676363823367308 15 0.00631645554053767 16 0.014365744016975055 17 0.024986332978941046 18 0.0012297524061223783 19 0.0026830961588124617 20 0.004695418277921808 21 0.0 22 4.4718269313541025E-4 23 0.0 24 0.0 25 0.0037451550550090607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1788978.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.24389755927625 #Duplication Level Percentage of deduplicated Percentage of total 1 82.47594163182318 60.40859419986104 2 12.086588790786799 17.705377424669695 3 3.077469292643292 6.76217536836551 4 1.0785581253295329 3.159912033734454 5 0.4679911448337163 1.7138747735424564 6 0.2412957464884033 1.060406456237142 7 0.13363458993446142 0.6851542750874908 8 0.08522543438254007 0.4993794388287667 9 0.06420159589771329 0.4232137601766746 >10 0.2590779010517264 3.2546549202116983 >50 0.01580572368721561 0.7921021792654458 >100 0.012071418350189867 1.7793354552845586 >500 0.0013748173657780623 0.6720404713283725 >1k 7.637874254322533E-4 1.0837792434066857 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3323 0.18574851116112104 No Hit TATCAACGCAGAGTACTTTTTTTTT 2311 0.12917990047949163 No Hit GTCCTACAGTGGACATTTCTAAATT 2220 0.12409319734507636 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2131 0.1191182898839449 No Hit CTGTAGGACGTGGAATATGGCAAGA 1989 0.11118079708079137 No Hit GGTATCAACGCAGAGTACTTTTTTT 1956 0.1093361684716078 No Hit CTTTAGGACGTGAAATATGGCGAGG 1871 0.10458485235704407 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 5.589783664192628E-5 0.0 11 0.0 0.0 0.0 5.589783664192628E-5 0.0 12 0.0 0.0 0.0 5.589783664192628E-5 0.0 13 0.0 0.0 0.0 5.589783664192628E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 485 0.0 13.929085 4 ACTGTCC 315 0.0 11.766878 8 GGTATCA 755 0.0 11.710213 1 AGGACCT 960 0.0 11.199875 5 CTAGACG 60 0.0058079176 11.100761 4 ATAGGAC 180 3.6379788E-12 11.092677 3 GCGCCAC 130 3.885616E-7 10.222218 13 TGGCGAG 1265 0.0 10.201479 18 GGACGTG 1230 0.0 10.053903 6 TATGTCG 85 6.644856E-4 10.050416 16 GTAGGAC 1355 0.0 10.034284 3 GGACCTG 930 0.0 10.023965 6 GGCGAGA 780 0.0 9.975763 19 AGGACGT 1250 0.0 9.971655 5 TAACACT 250 0.0 9.895536 4 CTAGGAC 290 0.0 9.835872 3 TGTAGGA 1485 0.0 9.795034 2 GTATCAA 1560 0.0 9.750439 1 TTAGGAC 835 0.0 9.678812 3 GACGTGA 690 0.0 9.642296 7 >>END_MODULE