FastQCFastQC Report
Thu 26 May 2016
SRR1512951_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512951_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110839
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40290.19087197081350116No Hit
GTCCTACAGTGGACATTTCTAAATT34630.16405798831649407No Hit
CTGTAGGACGTGGAATATGGCAAGA32010.1516458621429678No Hit
GTCCTAAAGTGTGTATTTCTCATTT30810.14596091885738324No Hit
CTTTAGGACGTGAAATATGGCGAGG27110.1284323437268309No Hit
GGTATCAACGCAGAGTACTTTTTTT23570.11166176103435649No Hit
TATCAACGCAGAGTACTTTTTTTTT23440.11104589217841815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC655.4798147E-513.14730310
GGCGAGG7850.013.06325119
TAGGACC6050.012.0886164
GGTATCA9500.011.9333791
TGGCGAG17550.011.26166218
GACGTGA9500.011.1938977
GTATTAG2400.011.1144221
GGACGTG17250.011.0635346
AGGACGT17600.011.0629815
ACGTGAA10850.010.9392568
TTAGGAC11800.010.8665843
TCCAACG1408.3273335E-910.8594618
CGTGAAA10600.010.8389449
GTTAGAC1252.2468885E-710.6379823
TATGGCG19300.010.5851616
ATGGCGA18800.010.56342417
GTCCTAC17650.010.2553131
TTTAGGA13450.010.1693162
TATACTG3550.010.1668455
TAGGACG19450.010.1574574
Produced by FastQC (version 0.11.5)