FastQCFastQC Report
Thu 26 May 2016
SRR1512951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110839
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48800.2311876936137716No Hit
GTCCTACAGTGGACATTTCTAAATT34590.16386849020697458No Hit
TATCAACGCAGAGTACTTTTTTTTT33450.15846779408566924No Hit
CTGTAGGACGTGGAATATGGCAAGA31560.1495140084108736No Hit
CTTTAGGACGTGAAATATGGCGAGG29510.13980223029799999No Hit
GTCCTAAAGTGTGTATTTCTCATTT29150.13809674731232463No Hit
GGTATCAACGCAGAGTACTTTTTTT29010.1374335039290064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGG801.9678664E-613.081575
GGCGAGG8750.012.69036119
GGTATCA10850.012.0023571
TGGCGAG17700.011.42095818
AATGTCC5750.011.0738918
TAGGACC6350.010.7873794
TAGAAAT7150.010.3787654
TATGGCG19800.010.35564916
TGTCCAC6900.010.32693810
AAATGTC6650.010.2911997
GGACGTG18600.010.1763996
GTCCTAC17400.010.1610691
ATGGCGA19900.010.16050517
CCGTGCC750.002642790310.1358519
GTCCTAA10150.010.1142441
ATGTCCA6400.010.0962579
AGGACGT18950.010.0410225
AGTGCGT951.6379831E-410.0039088
GACGTGG7900.09.9863357
TGTAGGA18500.09.9686572
Produced by FastQC (version 0.11.5)