##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512951_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2110839
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.22327567379606	31.0	30.0	34.0	26.0	34.0
2	30.376084580586202	31.0	31.0	34.0	26.0	34.0
3	30.472894901032245	31.0	31.0	34.0	27.0	34.0
4	33.93048309226805	37.0	35.0	37.0	30.0	37.0
5	33.554551057660014	37.0	35.0	37.0	28.0	37.0
6	33.408675412951915	37.0	35.0	37.0	28.0	37.0
7	33.26381026691283	37.0	35.0	37.0	27.0	37.0
8	33.14421658875926	37.0	35.0	37.0	26.0	37.0
9	34.570674030563204	39.0	35.0	39.0	27.0	39.0
10	34.45453585043672	39.0	35.0	39.0	27.0	39.0
11	34.474447364294484	39.0	35.0	39.0	27.0	39.0
12	34.32217757962592	39.0	35.0	39.0	26.0	39.0
13	34.298539111699185	39.0	35.0	39.0	26.0	39.0
14	35.3199775065744	40.0	36.0	41.0	25.0	41.0
15	35.338129056739994	40.0	36.0	41.0	25.0	41.0
16	35.31574885626047	40.0	36.0	41.0	25.0	41.0
17	35.29231978374476	40.0	36.0	41.0	25.0	41.0
18	35.220125741470575	40.0	36.0	41.0	25.0	41.0
19	35.20905857812936	40.0	35.0	41.0	25.0	41.0
20	35.1816832074829	40.0	35.0	41.0	25.0	41.0
21	35.119753330310836	40.0	35.0	41.0	24.0	41.0
22	35.075395139089245	40.0	35.0	41.0	24.0	41.0
23	34.967607666904016	40.0	35.0	41.0	23.0	41.0
24	34.94876681736504	40.0	35.0	41.0	23.0	41.0
25	34.856516768924585	40.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6122601502438876
1101	2	0.5340390230964722
1101	3	0.5778309508158941
1101	4	0.45221200501682546
1101	5	0.6063504029470437
1101	6	0.6888530333118084
1101	7	0.6927734675446899
1101	8	0.6993857508699719
1101	9	0.5196882180132079
1101	10	0.8721261435878205
1101	11	0.8847562695331632
1101	12	0.8315858753785079
1101	13	0.9216833512229385
1101	14	1.0337587331802993
1101	15	0.9809738613121581
1101	16	1.041382479516038
1101	17	0.955552203759197
1101	18	0.39452743593803774
1101	19	0.891733679956495
1101	20	0.7498890283843878
1101	21	1.0016265872430168
1101	22	0.8470995199857398
1101	23	0.8907837070168085
1101	24	0.8468914138727399
1101	25	0.800217113621315
1102	1	0.6605245433593367
1102	2	0.562507660805025
1102	3	0.509474349729679
1102	4	0.6197137023141792
1102	5	0.640915765657823
1102	6	0.6232514866498917
1102	7	0.6806245241262019
1102	8	0.7281227826425507
1102	9	0.9191722705778034
1102	10	0.830763560913212
1102	11	0.7869010902872375
1102	12	0.8258070822055501
1102	13	0.9062841692346382
1102	14	1.0738591671291928
1102	15	1.069028206562706
1102	16	0.962308402270601
1102	17	0.8036260699853628
1102	18	0.8657561050682432
1102	19	1.0300463133734468
1102	20	1.0353854253343684
1102	21	1.0875600001657375
1102	22	1.1116499565421307
1102	23	1.0349792403164884
1102	24	1.0658610109401039
1102	25	1.1636756971953446
1103	1	0.5195858424760225
1103	2	0.4647346589564805
1103	3	0.50170399000719
1103	4	0.5374575549376885
1103	5	0.5664317702349919
1103	6	0.6416879800232138
1103	7	0.583063854507138
1103	8	0.6406364330769136
1103	9	0.7869652256189283
1103	10	0.7880857260680685
1103	11	0.8821212946563008
1103	12	0.8685201013783654
1103	13	0.9356844952886476
1103	14	1.0042756976884775
1103	15	0.8595425118217435
1103	16	0.8515858699347376
1103	17	1.0412929019460506
1103	18	1.0123617465780512
1103	19	1.0994814992669646
1103	20	1.1195601090126388
1103	21	0.877739724164023
1103	22	1.0859787919097599
1103	23	1.1602948734490326
1103	24	1.1057854127428612
1103	25	1.0571220832130734
1104	1	0.3461477077272619
1104	2	0.5173759746366215
1104	3	0.4456548761020507
1104	4	0.32903065055884184
1104	5	0.24939088855296632
1104	6	0.5292205863681971
1104	7	0.5333047621958471
1104	8	0.46973745476160644
1104	9	0.6155096857565354
1104	10	0.581227707362622
1104	11	0.7076897413515866
1104	12	0.6325728654419152
1104	13	0.6557911227055087
1104	14	0.807605199185474
1104	15	0.9142283035478371
1104	16	0.90886615147096
1104	17	0.8119539467416459
1104	18	0.7357257576576401
1104	19	0.8119574695953702
1104	20	0.7643629907748988
1104	21	0.5422826036114969
1104	22	0.7663326304091527
1104	23	0.6376252962467532
1104	24	0.7461009421011369
1104	25	0.9303953706104835
1105	1	0.6939921589239049
1105	2	0.6861413895000403
1105	3	0.6613991830388422
1105	4	0.6400414823660867
1105	5	0.647505655889475
1105	6	0.8312054596138836
1105	7	0.8085952665031115
1105	8	0.9953045711795809
1105	9	1.018898013026579
1105	10	0.9942143790660651
1105	11	0.9211039915972634
1105	12	0.9990418933713912
1105	13	1.0183123031726709
1105	14	1.1064817789951178
1105	15	1.0319763716428696
1105	16	1.1066795334201416
1105	17	0.7626050322491409
1105	18	1.1000831717107928
1105	19	1.1444137468151752
1105	20	1.144447740017327
1105	21	0.998722149973112
1105	22	1.0998726358291648
1105	23	1.1238477958566762
1105	24	1.0707362548329584
1105	25	1.2114182195099161
1106	1	0.5794772748051678
1106	2	0.5648728495413451
1106	3	0.5651791442379164
1106	4	0.47231613451369725
1106	5	0.5603235210041007
1106	6	0.6470783761501693
1106	7	0.6903463147539384
1106	8	0.704255903136108
1106	9	0.6702894225849576
1106	10	0.729617095130898
1106	11	0.5604170483175466
1106	12	0.6838417348299757
1106	13	0.6081541789679861
1106	14	0.897999813081519
1106	15	0.8549575761404355
1106	16	0.9235120427553056
1106	17	0.831085537912827
1106	18	0.9742409204226448
1106	19	1.0201777704295054
1106	20	1.0635290342159678
1106	21	0.9359783403897026
1106	22	0.8808299339496131
1106	23	1.0761862817861285
1106	24	1.0418806735491941
1106	25	1.0334137553173335
1107	1	0.5787152909735767
1107	2	0.6202359351888909
1107	3	0.5830548064884056
1107	4	0.6485862834628904
1107	5	0.7340758868126258
1107	6	0.7149033801957927
1107	7	0.7192950162856491
1107	8	0.7537901290857718
1107	9	0.716820742723705
1107	10	0.8840644633667978
1107	11	0.8407736257929344
1107	12	0.7433049689342042
1107	13	0.8873435069728259
1107	14	1.0218527917136129
1107	15	1.0004912110777155
1107	16	0.9076131058740202
1107	17	0.8619075376454646
1107	18	0.9919793833682817
1107	19	0.9626304014900171
1107	20	0.9913382779311846
1107	21	0.9256391414929226
1107	22	1.0425189629303304
1107	23	0.9780476190220213
1107	24	1.1457760784278577
1107	25	1.1555432996045667
1108	1	0.6319039438469147
1108	2	0.47932351299229836
1108	3	0.5123984920884723
1108	4	0.6364346429386316
1108	5	0.648528344501706
1108	6	0.7419565438957321
1108	7	0.7364768533447617
1108	8	0.7449793892052341
1108	9	0.7079525064072456
1108	10	0.8216228977187754
1108	11	0.7790351045768418
1108	12	0.918868547428616
1108	13	0.869375390884116
1108	14	0.9084101424453621
1108	15	0.8192085257934849
1108	16	0.8228607418893006
1108	17	0.839025962853853
1108	18	0.7512575108166786
1108	19	0.854762463561741
1108	20	0.8857767410401038
1108	21	0.906312399455615
1108	22	1.035020084067206
1108	23	0.9277946870527813
1108	24	1.0342961603013876
1108	25	1.0452196793009065
1109	1	0.012398745104498232
1109	2	0.06470999993166515
1109	3	0.14540454877702658
1109	4	0.27808824845672575
1109	5	0.08929265712569645
1109	6	-0.11952969912253764
1109	7	0.04221003806470236
1109	8	0.19769022046020268
1109	9	0.369464834878805
1109	10	0.26646246554913944
1109	11	0.2622807716283049
1109	12	0.2970428928716444
1109	13	0.3196958219396038
1109	14	0.3118291053319453
1109	15	0.22575579771930165
1109	16	0.24158576451511493
1109	17	0.21408455564533568
1109	18	0.308129148722287
1109	19	0.5201364117984753
1109	20	0.4782992353223108
1109	21	0.4807206244201012
1109	22	0.49134795080325944
1109	23	0.4228982706192994
1109	24	-0.04096841914584104
1109	25	0.09600487355132259
1110	1	0.3035792804292541
1110	2	0.3382275361872331
1110	3	0.3239364913365499
1110	4	0.5047050052442046
1110	5	0.429017907059972
1110	6	0.44371237652210027
1110	7	0.4689704138010029
1110	8	0.5833492866617007
1110	9	0.6727203753550128
1110	10	0.7692421183767522
1110	11	0.6959928551600001
1110	12	0.913696384846034
1110	13	0.43316749250242736
1110	14	0.8146074258873099
1110	15	0.8607862978720746
1110	16	0.7467336644987768
1110	17	0.7785481473092233
1110	18	0.7140860044176662
1110	19	0.7427392099254746
1110	20	0.7859805842213206
1110	21	0.7636709146778173
1110	22	0.6312392018601898
1110	23	0.9045241046217285
1110	24	0.7711830584329107
1110	25	0.7932918343363298
1111	1	-0.22874150059465492
1111	2	-0.18482363868783835
1111	3	-0.099331951946656
1111	4	-0.17704876894310928
1111	5	0.16963626159046186
1111	6	0.004622223679966453
1111	7	0.20833985943337296
1111	8	0.2878759681302512
1111	9	-0.22408848117021307
1111	10	-0.08350306466474677
1111	11	0.15131882367768412
1111	12	0.1591551309429633
1111	13	0.2295140586803157
1111	14	0.4493024885414556
1111	15	0.5872723200514613
1111	16	0.23714221990917395
1111	17	0.500916541382999
1111	18	0.4323224908645642
1111	19	0.4015377383292744
1111	20	0.3382626714040171
1111	21	0.2850425981674647
1111	22	0.21269911082275428
1111	23	0.21050934765185758
1111	24	0.3270068330302962
1111	25	0.33521138278125306
1112	1	0.11010470270898054
1112	2	0.02077267815358752
1112	3	0.10618278864085795
1112	4	0.021546855893831207
1112	5	0.2572801907190936
1112	6	0.17828245913987928
1112	7	0.18463052956278858
1112	8	0.3540571662717227
1112	9	0.1470918361707021
1112	10	0.3390725225170428
1112	11	0.19449328834048174
1112	12	0.4760948581698088
1112	13	0.41815347825876614
1112	14	0.4187515310703702
1112	15	0.409784318636234
1112	16	0.06508712267548589
1112	17	0.26984873609269755
1112	18	0.26474888455491197
1112	19	0.32635230565551154
1112	20	0.4779942465058298
1112	21	0.5316632228743288
1112	22	0.4291736861616755
1112	23	0.5998432617437004
1112	24	0.6172278284910178
1112	25	0.6959904490137419
1113	1	-0.28667335273463124
1113	2	-0.5577161546158713
1113	3	-0.21511141109477805
1113	4	-0.25177223733668797
1113	5	-0.1515277798570196
1113	6	-0.2731119095301935
1113	7	-0.046150471544336824
1113	8	0.1048777764552753
1113	9	0.14736438737185154
1113	10	-0.2005057145060718
1113	11	0.001704705652215921
1113	12	-0.01629363099955583
1113	13	0.058041928765163675
1113	14	0.05456636000264581
1113	15	0.16279953001352254
1113	16	-0.2656603098758268
1113	17	-0.030119514785404533
1113	18	0.056911036144690286
1113	19	-0.01221450805174129
1113	20	0.11489591428993151
1113	21	-0.0865662313955653
1113	22	-0.14878478162024322
1113	23	-0.048590342109136486
1113	24	-0.3331915965132808
1113	25	-0.3095906645344044
1114	1	-0.6912488274267723
1114	2	-0.4365135205145698
1114	3	-0.5030823758862084
1114	4	-0.3285337046777883
1114	5	-0.6595759812988291
1114	6	-0.9655399870663999
1114	7	-0.7925086726071271
1114	8	-1.195451874354145
1114	9	-0.8419741784850174
1114	10	-0.9010921815161055
1114	11	-0.8566495609717677
1114	12	-0.8310856779169171
1114	13	-0.6813994046698539
1114	14	-1.8605984448531174
1114	15	-1.2145052972086319
1114	16	-1.1133378904699498
1114	17	-1.2441313409339898
1114	18	-1.2585683959554146
1114	19	-1.325675394040232
1114	20	-0.588217068855819
1114	21	-0.673132150369689
1114	22	-0.6548242091173861
1114	23	-0.7414234622661482
1114	24	-1.4593359332457183
1114	25	-1.2221644752183067
1115	1	-1.5004825646258446
1115	2	-1.3535813166596533
1115	3	-1.066156542788132
1115	4	-2.21422815112469
1115	5	-1.3922896641943865
1115	6	-1.381091368210953
1115	7	-1.0632119812800198
1115	8	-1.0600094351049236
1115	9	-1.3861980116640211
1115	10	-1.3107299859908892
1115	11	-1.210326838280146
1115	12	-1.8344614628087612
1115	13	-1.2773144556642748
1115	14	-2.085323474002422
1115	15	-1.9934156643154708
1115	16	-1.6216946137857136
1115	17	-1.5108101618649314
1115	18	-1.3753308162105
1115	19	-1.387185428052021
1115	20	-1.4329931924237584
1115	21	-1.6405888287115786
1115	22	-1.8811993656771762
1115	23	-2.0090923596433825
1115	24	-1.5215209009942825
1115	25	-1.3898132343438832
1116	1	-0.9916480596181394
1116	2	-1.1149757881838909
1116	3	-0.9269165322689901
1116	4	-0.9712038422767577
1116	5	-1.096869009637075
1116	6	-1.7586635576740335
1116	7	-1.080959236672932
1116	8	-1.387227807304761
1116	9	-1.4227456404123942
1116	10	-1.4847225188817248
1116	11	-1.3248328830827987
1116	12	-1.9495620798823623
1116	13	-1.648977683855577
1116	14	-1.8773613355053271
1116	15	-1.7042471395140026
1116	16	-2.050823469569764
1116	17	-1.9408199031829412
1116	18	-2.6087371476968073
1116	19	-2.006896225728916
1116	20	-1.842116788636865
1116	21	-1.81308127270826
1116	22	-3.2793699054128638
1116	23	-3.08258940020875
1116	24	-2.2821501419863424
1116	25	-2.7265156870818217
1201	1	0.6279308483339321
1201	2	0.5830371108859929
1201	3	0.5541228786876999
1201	4	0.5748924140676621
1201	5	0.6668296589329188
1201	6	0.6240756223997082
1201	7	0.665761847594105
1201	8	0.7061568088280197
1201	9	0.844626973697352
1201	10	0.6882257202429827
1201	11	0.7492516652664492
1201	12	0.5593894040231646
1201	13	0.515821288618028
1201	14	0.6138011818520468
1201	15	0.7479614549280456
1201	16	0.8131109263039704
1201	17	0.7592497881551381
1201	18	0.7871035737413408
1201	19	0.6392648027850498
1201	20	0.7864655069950643
1201	21	0.7741148236790139
1201	22	0.9154384265737789
1201	23	0.7857064090337502
1201	24	0.8247717266542764
1201	25	0.8280084329294297
1202	1	0.6296680934708974
1202	2	0.8024961038512402
1202	3	0.7221290272403991
1202	4	0.7576134464876318
1202	5	0.8034044988426317
1202	6	0.9044714741248754
1202	7	0.7699100281665565
1202	8	0.7347224311870661
1202	9	0.9584272283035347
1202	10	0.9223792376294782
1202	11	0.9987967353302807
1202	12	0.8746519381370206
1202	13	0.8605974789245536
1202	14	0.9785829785467612
1202	15	1.0826977991595257
1202	16	0.9678749518415017
1202	17	0.9495164459993717
1202	18	1.0520105998804183
1202	19	1.0845634956520627
1202	20	1.166048243919164
1202	21	1.2626493450991276
1202	22	1.0979795229638754
1202	23	1.040979175654364
1202	24	0.9777846513207464
1202	25	1.0340295317249613
1203	1	0.4582410148093423
1203	2	0.33395064678381203
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2211	12	-0.22976525243655743
2211	13	-0.23796436940839527
2211	14	-1.3535296076401693
2211	15	-0.7441796815359325
2211	16	-0.3137626561099651
2211	17	-0.07014243384994501
2211	18	-0.4521912045411298
2211	19	-0.30853644597378604
2211	20	-0.548290429676058
2211	21	-0.5429901719485528
2211	22	-0.3742850738200403
2211	23	-0.3504680513541132
2211	24	-0.5170294523562049
2211	25	-0.5090070873966255
2212	1	-0.4801162115320139
2212	2	-0.45707961363475746
2212	3	-0.26414940630285244
2212	4	-0.21465796217002264
2212	5	-0.3502188670165367
2212	6	-0.3373895192124934
2212	7	-0.34675244839542074
2212	8	-0.6492225970544823
2212	9	-0.6497897722673827
2212	10	-0.6750749296288703
2212	11	-0.6194593092881746
2212	12	-0.6328395343358082
2212	13	-0.6254208541721056
2212	14	-0.5944579724210399
2212	15	-0.5132031916855126
2212	16	-0.4787986604972545
2212	17	-0.6879703678498501
2212	18	-0.4692982559964065
2212	19	-0.5096106166012007
2212	20	-0.6771988529541275
2212	21	-0.46318938827690204
2212	22	-0.5574583876543073
2212	23	-1.001599050098335
2212	24	-0.7975954286076146
2212	25	-0.9229999183111204
2213	1	-0.46196626217288284
2213	2	-0.6259283487771903
2213	3	-0.555637083691412
2213	4	-0.5661623452245408
2213	5	-0.3267945547428255
2213	6	-0.2834053342976546
2213	7	-0.745791581563445
2213	8	-0.4610488406661659
2213	9	-0.7172745509027578
2213	10	-0.535864199585447
2213	11	-0.5037936729012529
2213	12	-0.6859673355907674
2213	13	-0.49095916078801594
2213	14	-0.44231635843252093
2213	15	-0.633270072557238
2213	16	-0.8901262079657926
2213	17	-0.8913436609033241
2213	18	-0.9736121886382278
2213	19	-1.1973791005183614
2213	20	-0.705928814886164
2213	21	-1.3841263316639
2213	22	-0.761024494361024
2213	23	-0.9334346942851113
2213	24	-1.0888342672436906
2213	25	-1.1969815711825191
2214	1	-1.1917212250801121
2214	2	-1.1138896380243395
2214	3	-0.9374605554401931
2214	4	-1.602918482621483
2214	5	-1.4417095036418175
2214	6	-2.8382930390113614
2214	7	-1.4641572057175587
2214	8	-2.029116866254139
2214	9	-1.5744113728375737
2214	10	-1.6739781877885562
2214	11	-1.4117732447324656
2214	12	-1.4785836873015015
2214	13	-1.6818777784380856
2214	14	-1.7920025112293487
2214	15	-2.2087694600792034
2214	16	-1.5826085179790823
2214	17	-1.6303471568453674
2214	18	-1.6735826352348866
2214	19	-3.537041755874448
2214	20	-2.03749104678365
2214	21	-2.5172691927391995
2214	22	-2.231297326181128
2214	23	-2.3257429336057456
2214	24	-1.6730450269764319
2214	25	-1.4252445268898768
2215	1	-1.4182930518541639
2215	2	-1.2665386614519285
2215	3	-1.2142549072092663
2215	4	-1.1314534092858324
2215	5	-1.854442618093838
2215	6	-1.801752873249761
2215	7	-1.7579827226977756
2215	8	-2.5723997345622855
2215	9	-2.1304816204649484
2215	10	-1.6350689116194204
2215	11	-2.3170509910617767
2215	12	-3.0254351538192985
2215	13	-2.605802143363462
2215	14	-2.5211329850514304
2215	15	-3.464027881869473
2215	16	-2.9442998640991505
2215	17	-2.5517111179464393
2215	18	-3.24147462082151
2215	19	-3.908520688222943
2215	20	-3.370929721627789
2215	21	-2.5017488551258396
2215	22	-3.4917105199613587
2215	23	-3.4168639911069825
2215	24	-2.570075294279704
2215	25	-2.6091889581042835
2216	1	-1.4906004577605074
2216	2	-1.3584311613016418
2216	3	-1.691193355829835
2216	4	-1.3071228612553583
2216	5	-1.3259464295438974
2216	6	-1.6687000093779645
2216	7	-3.0278925316307834
2216	8	-2.0582818559430542
2216	9	-2.21560688152649
2216	10	-2.1277464867964184
2216	11	-2.5419955791129674
2216	12	-2.0229507787284007
2216	13	-2.534119636862375
2216	14	-2.577498038776298
2216	15	-2.696039408330499
2216	16	-2.5012092444743033
2216	17	-2.6149555917801663
2216	18	-2.5375823071326735
2216	19	-2.7212145330850035
2216	20	-3.017864078587394
2216	21	-2.9105642514061145
2216	22	-3.1213932901903583
2216	23	-2.285308849673193
2216	24	-2.5015708060572877
2216	25	-2.6225621932118983
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64992.0
3	0.0
4	1059.0
5	9148.0
6	10389.0
7	8058.0
8	6471.0
9	5881.0
10	4910.0
11	4451.0
12	4021.0
13	3755.0
14	3662.0
15	3579.0
16	3626.0
17	3847.0
18	4281.0
19	5042.0
20	6387.0
21	8360.0
22	10513.0
23	12580.0
24	15111.0
25	17132.0
26	19452.0
27	21269.0
28	24198.0
29	28961.0
30	36264.0
31	46380.0
32	58742.0
33	75832.0
34	99254.0
35	130012.0
36	178442.0
37	277968.0
38	896810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.992992948380895	19.76942490402582	13.633572780835602	27.60400936675768
2	13.260840831536655	20.815609338277337	40.59660637310567	25.326943457080336
3	16.703358238122377	25.0606038641507	30.515496444778595	27.72054145294833
4	11.070708755674325	17.671857576879134	38.226667797700934	33.03076586974561
5	12.329364622194912	38.354314734770526	36.487678789938975	12.82864185309558
6	31.11062224658116	38.34641256668418	18.04317853817284	12.499786648561821
7	26.983968254043482	31.979368730157	22.863259306512177	18.173403709287342
8	24.267628102562643	37.93215429434256	20.659683456238785	17.140534146856016
9	25.724764936938115	15.175188754323374	20.41262650177533	38.687419806963184
10	14.04967536009182	29.665305502990293	35.21098036983813	21.07403876707976
11	33.68109710222654	22.71756042002649	25.058747614562588	18.542594863184387
12	22.40147487412067	25.161557184301937	32.46310327198606	19.973864669591336
13	28.317045772727766	22.317835080217048	26.155594015476304	23.209525131578886
14	20.21398872018011	22.42110569524777	28.11682724743589	29.248078337136228
15	22.234514217706348	31.474862579701878	23.712427949691993	22.57819525289978
16	21.65171104391163	28.692383577844893	27.92937673480644	21.726528643437042
17	21.095502995649284	29.010006980291692	28.26193580121949	21.632554222839534
18	20.929331327150553	28.159074629241506	30.022342155231552	20.889251888376396
19	22.71361973886183	27.43708427296329	28.075006158919063	21.774289829255814
20	22.833703348901466	27.877759363857486	28.44481651302256	20.84372077421849
21	22.285883210143478	27.239043903973503	27.769876740662696	22.705196145220324
22	22.3817963291165	28.387594056737004	27.933601379933076	21.297008234213415
23	21.69930695738231	27.98245435341526	28.21994308418888	22.098295605013558
24	22.30391181503442	27.324008118110548	28.85795012023672	21.514129946618308
25	22.15314221390714	27.650885440626276	28.378349568424976	21.8176227770416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	179.0
1	179.0
2	333.5
3	488.0
4	488.0
5	488.0
6	1311.5
7	2135.0
8	2135.0
9	2135.0
10	3251.5
11	4368.0
12	4368.0
13	4368.0
14	7582.5
15	10797.0
16	10797.0
17	10797.0
18	19482.5
19	28168.0
20	28168.0
21	28168.0
22	44562.0
23	60956.0
24	60956.0
25	60956.0
26	85639.0
27	110322.0
28	110322.0
29	110322.0
30	140860.5
31	171399.0
32	171399.0
33	171399.0
34	193141.5
35	214884.0
36	214884.0
37	214884.0
38	230023.5
39	245163.0
40	245163.0
41	245163.0
42	255627.0
43	266091.0
44	266091.0
45	266091.0
46	268229.5
47	270368.0
48	270368.0
49	270368.0
50	258133.0
51	245898.0
52	245898.0
53	245898.0
54	217890.5
55	189883.0
56	189883.0
57	189883.0
58	161323.5
59	132764.0
60	132764.0
61	132764.0
62	107437.5
63	82111.0
64	82111.0
65	82111.0
66	63053.5
67	43996.0
68	43996.0
69	43996.0
70	31892.5
71	19789.0
72	19789.0
73	19789.0
74	13668.0
75	7547.0
76	7547.0
77	7547.0
78	5044.5
79	2542.0
80	2542.0
81	2542.0
82	1645.0
83	748.0
84	748.0
85	748.0
86	476.0
87	204.0
88	204.0
89	204.0
90	118.0
91	32.0
92	32.0
93	32.0
94	19.0
95	6.0
96	6.0
97	6.0
98	3.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07949445694342391
2	0.0
3	0.0
4	0.03202518050879295
5	0.07532549853399526
6	0.07783634848512842
7	0.039699853944332085
8	0.07077754390552761
9	0.051448736734540146
10	0.1112827648153175
11	0.005448070648685191
12	0.01354911483064317
13	0.006063939504623517
14	0.007911546072438495
15	0.007627298908159268
16	0.017670698712691967
17	0.02899321075648119
18	0.001468610348776008
19	0.0037425876630098266
20	0.005542819703444933
21	2.3687263689935615E-4
22	5.684943285584548E-4
23	9.474905475974246E-5
24	1.421235821396137E-4
25	0.0038847112451494406
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2110839.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.26264411666214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.4246195146962	62.69595473704494
2	10.984795169684729	16.315198687614526
3	2.6082896317820783	5.8109545403463665
4	0.8521272627384452	2.5312489461940246
5	0.36433670008563684	1.3528303348549362
6	0.19371992318041842	0.8631692228072726
7	0.11907135227941885	0.6189787421171334
8	0.07985461781068302	0.4744172050837459
9	0.07019590398172779	0.46916400922581947
>10	0.2721356482867737	3.559976641584967
>50	0.015711918790960813	0.8071884536287889
>100	0.012013946381701661	1.7379220886309346
>500	0.001979198353861063	0.973105755918538
>1k	0.0011492119474029033	1.7898906349480233
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4880	0.2311876936137716	No Hit
GTCCTACAGTGGACATTTCTAAATT	3459	0.16386849020697458	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3345	0.15846779408566924	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3156	0.1495140084108736	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2951	0.13980223029799999	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2915	0.13809674731232463	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2901	0.1374335039290064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.737452737987123E-5	0.0
10	0.0	9.474905475974246E-5	0.0	4.737452737987123E-5	0.0
11	0.0	9.474905475974246E-5	0.0	4.737452737987123E-5	0.0
12	0.0	9.474905475974246E-5	0.0	4.737452737987123E-5	0.0
13	0.0	9.474905475974246E-5	0.0	9.474905475974246E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACGG	80	1.9678664E-6	13.08157	5
GGCGAGG	875	0.0	12.690361	19
GGTATCA	1085	0.0	12.002357	1
TGGCGAG	1770	0.0	11.420958	18
AATGTCC	575	0.0	11.073891	8
TAGGACC	635	0.0	10.787379	4
TAGAAAT	715	0.0	10.378765	4
TATGGCG	1980	0.0	10.355649	16
TGTCCAC	690	0.0	10.326938	10
AAATGTC	665	0.0	10.291199	7
GGACGTG	1860	0.0	10.176399	6
GTCCTAC	1740	0.0	10.161069	1
ATGGCGA	1990	0.0	10.160505	17
CCGTGCC	75	0.0026427903	10.135851	9
GTCCTAA	1015	0.0	10.114244	1
ATGTCCA	640	0.0	10.096257	9
AGGACGT	1895	0.0	10.041022	5
AGTGCGT	95	1.6379831E-4	10.003908	8
GACGTGG	790	0.0	9.986335	7
TGTAGGA	1850	0.0	9.968657	2
>>END_MODULE