FastQCFastQC Report
Thu 26 May 2016
SRR1512950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019623
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT22070.1092778206625692No Hit
GTATCAACGCAGAGTACTTTTTTTT21460.10625745498045921No Hit
GTCCTAAAGTGTGTATTTCTCATTT20520.10160312097851926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCG500.001497035313.30327816
TAGGACC5250.011.7595594
TGCGTTG2650.011.11622112
TATGCCG600.005886509511.0808475
ATACCGT600.005899545311.0775556
CGTTGAT2850.010.6695614
AGGACCT9400.010.5082825
GTAGGAC13600.010.4758513
GTCCTAC12550.010.4729281
CTAGGAC2950.010.3030293
AGGACGT12350.010.2284765
GTCCTAA6800.010.2246081
CTGCGTT2800.010.17351711
GGCGAGG5800.010.149878519
GGACCTG9700.010.082376
GGACGTG12150.010.08116156
TGGCGAG12500.010.03447318
AAGGCGT1803.9835868E-1010.022556
GCGTTGA2850.010.00271313
GTATTAA2201.8189894E-129.9572141
Produced by FastQC (version 0.11.5)