##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512950_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2019623 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.257799104090218 31.0 28.0 33.0 16.0 34.0 2 28.279056041647376 31.0 28.0 34.0 16.0 34.0 3 28.44497661197164 31.0 28.0 34.0 16.0 34.0 4 31.874689484126492 35.0 32.0 37.0 19.0 37.0 5 31.96962948035351 35.0 33.0 37.0 19.0 37.0 6 32.07414750178622 35.0 33.0 37.0 19.0 37.0 7 31.823090745153923 35.0 32.0 37.0 17.0 37.0 8 31.82542831013511 35.0 32.0 37.0 17.0 37.0 9 33.10303903253231 38.0 33.0 39.0 17.0 39.0 10 32.951927166604854 37.0 33.0 39.0 17.0 39.0 11 33.034523274888436 38.0 33.0 39.0 17.0 39.0 12 32.92810143279216 37.0 33.0 39.0 16.0 39.0 13 32.92496272819234 38.0 33.0 39.0 15.0 39.0 14 33.82294121229556 39.0 33.0 41.0 11.0 41.0 15 33.75450022108087 39.0 33.0 41.0 11.0 41.0 16 33.630227027519496 38.0 33.0 41.0 10.0 41.0 17 33.541148026141514 38.0 33.0 41.0 10.0 41.0 18 33.23101143134139 38.0 32.0 41.0 10.0 41.0 19 33.47191579814648 39.0 33.0 41.0 9.0 41.0 20 33.56586699596905 39.0 33.0 41.0 2.0 41.0 21 33.48612488568411 39.0 33.0 41.0 2.0 41.0 22 33.36862424323748 39.0 33.0 41.0 2.0 41.0 23 33.079309851393056 39.0 32.0 41.0 2.0 41.0 24 33.08301103720843 39.0 33.0 41.0 2.0 41.0 25 32.969266046187826 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8143953076028758 1101 2 1.0752064963399555 1101 3 1.0427244073131767 1101 4 1.1255535521982019 1101 5 0.8261720222512849 1101 6 1.1311211820160167 1101 7 1.1617744468502345 1101 8 1.0113897925524924 1101 9 0.8693214261669127 1101 10 0.9880498879516537 1101 11 1.1799429685804057 1101 12 1.3737367403093756 1101 13 1.092156426693812 1101 14 1.1612530430013734 1101 15 0.8951657232706438 1101 16 1.3755277398445216 1101 17 1.5392331710192693 1101 18 1.7220801737544065 1101 19 1.0605739679404564 1101 20 1.3550324897865522 1101 21 1.3757162564161476 1101 22 1.4010279155426133 1101 23 1.610392814485003 1101 24 1.2739766416981197 1101 25 1.4277985136687548 1102 1 0.8016957126358655 1102 2 0.7668132984285592 1102 3 0.8635549689400897 1102 4 0.9742900393267782 1102 5 0.8436457156538921 1102 6 0.7011564458268182 1102 7 0.9122448450256577 1102 8 0.9085381240066326 1102 9 1.041137088641129 1102 10 1.1228747631927618 1102 11 1.066973437232086 1102 12 1.187812985137306 1102 13 1.0413077066442966 1102 14 1.1549615476668293 1102 15 1.3979090063237223 1102 16 1.251117118066226 1102 17 0.9849163720384055 1102 18 1.4652281302255545 1102 19 1.5549556441143935 1102 20 1.4148792604382265 1102 21 1.1745615142939982 1102 22 1.4299950942579613 1102 23 1.562486426675548 1102 24 1.3996467953640916 1102 25 1.3056393308234604 1103 1 0.43498965706465853 1103 2 0.5828848674376239 1103 3 0.561031855749917 1103 4 0.7468832262740897 1103 5 0.34861110684345675 1103 6 0.6883179857969353 1103 7 0.600225986135829 1103 8 0.5295088829714203 1103 9 0.8958252808297829 1103 10 0.7857793746811126 1103 11 0.8416860591692839 1103 12 0.9281494466863407 1103 13 0.9416116350155548 1103 14 1.1250546176420286 1103 15 1.0980062673845552 1103 16 1.2050276199711334 1103 17 0.9742343798690598 1103 18 1.345848689640917 1103 19 1.3799741356620387 1103 20 1.3858189098006548 1103 21 1.1338782180101248 1103 22 1.1358248844149799 1103 23 1.5099927307253722 1103 24 1.4144840349020527 1103 25 1.2155200391866146 1104 1 0.6074196744445821 1104 2 0.5309879248985077 1104 3 0.5740578681779027 1104 4 0.8252881400130754 1104 5 0.5528680812308764 1104 6 0.5140764114873875 1104 7 0.703882640875559 1104 8 0.7389076277505566 1104 9 0.7130300055907526 1104 10 0.9028556813488038 1104 11 0.8670670723135032 1104 12 0.8500686838176463 1104 13 0.8290478190261084 1104 14 0.993180726694149 1104 15 1.0656909489461128 1104 16 0.8384939263321343 1104 17 0.9951863663315876 1104 18 1.2149521038618616 1104 19 1.0528416596657024 1104 20 1.0347198442480305 1104 21 1.2219422453770008 1104 22 1.166473252150361 1104 23 1.3014703579099418 1104 24 1.1929752465314252 1104 25 1.0398073634124074 1105 1 0.792604972950361 1105 2 1.0407413433948172 1105 3 1.139851372757633 1105 4 1.0829107949301537 1105 5 0.8786541355481887 1105 6 0.7220456298179414 1105 7 0.9620688461868916 1105 8 0.9746910316408304 1105 9 0.8591052509464845 1105 10 1.1505333068109564 1105 11 1.1011921426377853 1105 12 1.0611436442429039 1105 13 1.0737225833274167 1105 14 1.2055179864585597 1105 15 1.5112160681484 1105 16 1.4875209748387732 1105 17 1.3705596921738632 1105 18 1.3471106950126455 1105 19 1.3906175498056967 1105 20 1.3856744378052355 1105 21 1.4764574371428552 1105 22 1.1035323031292066 1105 23 1.3019298799993209 1105 24 1.3997150568251016 1105 25 1.329562717141151 1106 1 0.8036203408282425 1106 2 0.8416339536244699 1106 3 0.870676093251884 1106 4 0.9201672625366619 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1.1069273925810563 1107 18 1.4626675737627153 1107 19 1.3193599166792112 1107 20 1.2218754530355582 1107 21 1.1049589224701748 1107 22 1.1617018833644082 1107 23 1.082345771648356 1107 24 1.2751194737023042 1107 25 1.2869813741215381 1108 1 0.42905396129302886 1108 2 0.4423017366358408 1108 3 0.2499965571395819 1108 4 0.5077432304434986 1108 5 0.34737324110996326 1108 6 0.588980785149495 1108 7 0.6114142267162528 1108 8 0.5769437606769188 1108 9 0.853446863598144 1108 10 0.7163495132283231 1108 11 0.582108320781991 1108 12 0.6227843963465887 1108 13 0.6324207487257993 1108 14 0.8382021008833931 1108 15 0.7691972291353437 1108 16 0.6051696239034854 1108 17 0.737380891263868 1108 18 0.9381621074880613 1108 19 0.7126896159581335 1108 20 0.6626810345011407 1108 21 0.6955479799763182 1108 22 0.5565509795621892 1108 23 0.7416214960114687 1108 24 1.012189867239087 1108 25 1.0496828086215473 1109 1 0.27445576702801233 1109 2 0.3650074315921543 1109 3 0.39714509348382876 1109 4 0.6973835504905033 1109 5 0.4665769724485713 1109 6 0.45807610140932553 1109 7 0.47702042784999676 1109 8 0.7006166604751982 1109 9 0.9970192680747303 1109 10 1.0710727383097947 1109 11 0.557926206983069 1109 12 0.7879819319413741 1109 13 0.8848539436863803 1109 14 0.6723093802845668 1109 15 0.943137622382693 1109 16 0.9040191809721421 1109 17 0.6489272821946912 1109 18 -0.9503835856007612 1109 19 0.5334832390069266 1109 20 0.7047950497762372 1109 21 0.9578007817351022 1109 22 1.0166649534640015 1109 23 0.6421449439393001 1109 24 1.0499919515035572 1109 25 1.0197077711341223 1110 1 0.3574793007786674 1110 2 0.3786572292239505 1110 3 0.4857477266401844 1110 4 0.16204417284787453 1110 5 0.43718326782683903 1110 6 0.38638191963877233 1110 7 0.3732829916275868 1110 8 0.2860892896927538 1110 9 0.6255305555882131 1110 10 0.7461647568828553 1110 11 0.5579730319065845 1110 12 0.012218132432209927 1110 13 0.24058887130863127 1110 14 0.4424963598974898 1110 15 0.6502433567641788 1110 16 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-2.5358244616012833 2215 16 -2.1283367455416524 2215 17 -2.6489356176872825 2215 18 -1.417662137517219 2215 19 -1.9642448355894615 2215 20 -2.0342268334753477 2215 21 -1.764735145409059 2215 22 -1.4278753721791517 2215 23 -1.646124736008236 2215 24 -1.6206968294475814 2215 25 -1.599736691160544 2216 1 -2.4692453130067307 2216 2 -2.4637052126416634 2216 3 -2.885629486947934 2216 4 -3.6557089122347506 2216 5 -2.7974399515790935 2216 6 -2.9225924635825784 2216 7 -2.731547264953697 2216 8 -3.373761107690303 2216 9 -3.6211286298120626 2216 10 -3.1166332187348544 2216 11 -3.9047992882848845 2216 12 -4.262278952493478 2216 13 -4.561068018142446 2216 14 -4.059040440074412 2216 15 -3.860731444884955 2216 16 -3.117474608344061 2216 17 -3.4436606271835473 2216 18 -3.354345296335005 2216 19 -3.6823095378571225 2216 20 -3.3801737040981905 2216 21 -3.740372480066508 2216 22 -4.696751429494078 2216 23 -4.350670617758009 2216 24 -4.460576246125257 2216 25 -4.3270669218551205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 126235.0 3 4.0 4 1045.0 5 5713.0 6 7245.0 7 6295.0 8 5668.0 9 5356.0 10 4953.0 11 4636.0 12 4529.0 13 4531.0 14 4456.0 15 4452.0 16 4644.0 17 4925.0 18 5588.0 19 6763.0 20 8383.0 21 10824.0 22 13631.0 23 16258.0 24 19141.0 25 21704.0 26 24084.0 27 26375.0 28 30189.0 29 35302.0 30 42900.0 31 53305.0 32 66489.0 33 83468.0 34 106739.0 35 138073.0 36 184745.0 37 286722.0 38 644253.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48694357653382 18.277700924622955 12.284794603318467 25.95056089552476 2 15.241696085419477 20.244972724395282 40.25386893543163 24.259462254753604 3 18.782189271679187 24.406885272210165 29.392377501216 27.418547954894652 4 12.608145423926098 16.658469234865088 37.72799881124856 33.00538652996025 5 13.506835866392382 37.46899824007585 35.60401359792792 13.420152295603854 6 32.12941879111007 36.729586970366576 18.08689657768089 13.054097660842462 7 28.004167826322078 31.264788701704273 22.408207289932342 18.322836182041303 8 25.349215790472012 36.0759164546568 20.68248936318789 17.8923783916833 9 26.67608285786025 14.980658344298806 19.817681804382588 38.52557699345836 10 15.057610841317368 28.47118566508752 34.568188593915124 21.903014899679977 11 34.52087941039845 22.19969473995945 24.479240435366982 18.800185414275116 12 23.107905930525035 24.535776243530332 31.545771632864195 20.810546193080434 13 28.99324298893245 21.710538045082398 25.460916901243834 23.835302064741317 14 21.38273730338837 21.46955893817091 27.320534777686095 29.82716898075462 15 23.446333699671978 29.888528383485735 23.225589489069026 23.439548427773257 16 23.01083490902896 27.563844149258077 26.76813849349301 22.657182448219952 17 22.429776018209356 27.85598326451008 27.275500673044828 22.438740044235736 18 22.36571151597813 27.2125515493424 28.50227012871414 21.919466805965328 19 23.780110017299467 26.800212169852767 27.006290311157688 22.413387501690078 20 23.897291335572188 26.9721922734249 27.281475167976023 21.84904122302689 21 23.50501851794988 26.485720030785657 26.649206932518194 23.360054518746267 22 23.675809737752036 27.29470680465959 26.650997358404226 22.378486099184148 23 23.094630118641753 27.105995083172047 26.87639783913137 22.92297695905483 24 23.40085190172376 26.627005017216813 27.374610436464682 22.597532644594743 25 23.289262812576144 26.794830397169555 27.10762940350279 22.808277386751506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 203.0 1 203.0 2 316.0 3 429.0 4 429.0 5 429.0 6 814.0 7 1199.0 8 1199.0 9 1199.0 10 1891.0 11 2583.0 12 2583.0 13 2583.0 14 5000.0 15 7417.0 16 7417.0 17 7417.0 18 14081.0 19 20745.0 20 20745.0 21 20745.0 22 33309.5 23 45874.0 24 45874.0 25 45874.0 26 66189.0 27 86504.0 28 86504.0 29 86504.0 30 111057.5 31 135611.0 32 135611.0 33 135611.0 34 157664.0 35 179717.0 36 179717.0 37 179717.0 38 197971.5 39 216226.0 40 216226.0 41 216226.0 42 230802.5 43 245379.0 44 245379.0 45 245379.0 46 253810.0 47 262241.0 48 262241.0 49 262241.0 50 257188.0 51 252135.0 52 252135.0 53 252135.0 54 229830.0 55 207525.0 56 207525.0 57 207525.0 58 181124.5 59 154724.0 60 154724.0 61 154724.0 62 127387.5 63 100051.0 64 100051.0 65 100051.0 66 78306.0 67 56561.0 68 56561.0 69 56561.0 70 41693.0 71 26825.0 72 26825.0 73 26825.0 74 19009.0 75 11193.0 76 11193.0 77 11193.0 78 7737.0 79 4281.0 80 4281.0 81 4281.0 82 2903.5 83 1526.0 84 1526.0 85 1526.0 86 1012.0 87 498.0 88 498.0 89 498.0 90 311.0 91 124.0 92 124.0 93 124.0 94 80.0 95 36.0 96 36.0 97 36.0 98 26.0 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35288764289176744 2 0.03891815452685971 3 0.03490750501454975 4 0.03456090567397974 5 0.03891815452685971 6 0.01728045283698987 7 0.017082396070949874 8 0.022578471328559835 9 0.020102761753059854 10 0.01906296373134986 11 0.01787462313510987 12 0.019409563071919857 13 0.01916199211436986 14 0.026787177606909802 15 0.026935720181439804 16 0.020251304327589852 17 0.02183575845590984 18 0.0938789071029593 19 0.024063897073859825 20 0.02158818749835984 21 0.02480660994650982 22 0.01926102049738986 23 0.021538673306849842 24 0.018617336007759864 25 0.021538673306849842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2019623.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.71041012975023 #Duplication Level Percentage of deduplicated Percentage of total 1 81.89323581470477 59.54490762939535 2 12.080624624000977 17.567743420693418 3 3.319507437144076 7.240882415505055 4 1.2323898619517053 3.584302892090191 5 0.535090234916281 1.9453315218593596 6 0.2713508726783832 1.1838019944906528 7 0.17604447375483634 0.8960186121453141 8 0.1057133528700592 0.6149168994698412 9 0.07323588058236938 0.4792509822021741 >10 0.28726246021209056 3.45846009045907 >50 0.01407361744433146 0.7033484705707577 >100 0.009833590394827873 1.516813718755363 >500 0.0010918528968735632 0.5708074271984849 >1k 5.459264484367134E-4 0.6934139251649956 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2207 0.1092778206625692 No Hit GTATCAACGCAGAGTACTTTTTTTT 2146 0.10625745498045921 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2052 0.10160312097851926 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.951419150999964E-5 0.0 10 0.0 0.0 0.0 4.951419150999964E-5 0.0 11 0.0 0.0 0.0 4.951419150999964E-5 0.0 12 0.0 0.0 0.0 4.951419150999964E-5 0.0 13 0.0 0.0 0.0 4.951419150999964E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGTCG 50 0.0014970353 13.303278 16 TAGGACC 525 0.0 11.759559 4 TGCGTTG 265 0.0 11.116221 12 TATGCCG 60 0.0058865095 11.080847 5 ATACCGT 60 0.0058995453 11.077555 6 CGTTGAT 285 0.0 10.66956 14 AGGACCT 940 0.0 10.508282 5 GTAGGAC 1360 0.0 10.475851 3 GTCCTAC 1255 0.0 10.472928 1 CTAGGAC 295 0.0 10.303029 3 AGGACGT 1235 0.0 10.228476 5 GTCCTAA 680 0.0 10.224608 1 CTGCGTT 280 0.0 10.173517 11 GGCGAGG 580 0.0 10.1498785 19 GGACCTG 970 0.0 10.08237 6 GGACGTG 1215 0.0 10.0811615 6 TGGCGAG 1250 0.0 10.034473 18 AAGGCGT 180 3.9835868E-10 10.02255 6 GCGTTGA 285 0.0 10.002713 13 GTATTAA 220 1.8189894E-12 9.957214 1 >>END_MODULE