FastQCFastQC Report
Thu 26 May 2016
SRR1512950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019623
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26000.12873689792599907No Hit
GTCCTACAGTGGACATTTCTAAATT21330.10561377049082923No Hit
CTGTAGGACGTGGAATATGGCAAGA20240.10021672361623926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAA500.001497077813.30322810
TAGGACC5650.011.9560664
GCACCGT802.8335045E-511.8887876
TGGCGAG11550.011.33878818
GGCGAGG5250.010.66503319
TATGTCG909.5970994E-510.54687516
TAGGACT2550.010.4471124
GGCGAGA7550.010.43279519
GTAGGAC12350.010.3142853
CTAGGAC2700.010.2102293
ACTAGAC1409.600626E-810.18508153
TGTAGGA13450.010.1065822
TATGGCG13400.09.98804816
AGGACCT11200.09.9390815
CTTACAC2400.09.9021633
TTTATAC2700.09.8581523
ATGGCGA13750.09.80284117
TTACACT3600.09.7786224
TAGGACA5000.09.704624
GTCTAGA2650.09.6854361
Produced by FastQC (version 0.11.5)