##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512950_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2019623 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.468301262166257 31.0 31.0 34.0 27.0 34.0 2 30.592766075648772 31.0 31.0 34.0 27.0 34.0 3 30.703654097819246 31.0 31.0 34.0 27.0 34.0 4 34.2067167981351 37.0 35.0 37.0 30.0 37.0 5 33.87542675043807 37.0 35.0 37.0 30.0 37.0 6 33.751668009326494 37.0 35.0 37.0 29.0 37.0 7 33.61560202077318 37.0 35.0 37.0 28.0 37.0 8 33.49589651137861 37.0 35.0 37.0 28.0 37.0 9 34.96257519348908 39.0 35.0 39.0 28.0 39.0 10 34.84293603311113 39.0 35.0 39.0 27.0 39.0 11 34.89220215852166 39.0 35.0 39.0 27.0 39.0 12 34.741218534350224 39.0 35.0 39.0 27.0 39.0 13 34.745082621855666 39.0 35.0 39.0 27.0 39.0 14 35.79590349287961 40.0 36.0 41.0 27.0 41.0 15 35.80851277688955 40.0 36.0 41.0 27.0 41.0 16 35.77781991985633 40.0 36.0 41.0 27.0 41.0 17 35.74179686010706 40.0 36.0 41.0 27.0 41.0 18 35.659783533857556 40.0 36.0 41.0 27.0 41.0 19 35.63478134285458 40.0 36.0 41.0 27.0 41.0 20 35.60868884935456 40.0 36.0 41.0 27.0 41.0 21 35.55078249752553 40.0 36.0 41.0 27.0 41.0 22 35.516294872854985 40.0 36.0 41.0 27.0 41.0 23 35.4042917910917 40.0 36.0 41.0 26.0 41.0 24 35.39053575840639 40.0 36.0 41.0 26.0 41.0 25 35.29158461752515 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7316222960987062 1101 2 0.774756899731269 1101 3 0.7499822488429047 1101 4 0.6174527882801257 1101 5 0.8167319457381126 1101 6 0.8794899821289874 1101 7 0.8705426969741197 1101 8 0.8867017462441922 1101 9 0.7574609289315433 1101 10 1.091555193376749 1101 11 1.1913150817454508 1101 12 1.1583641662442261 1101 13 1.2136377825395783 1101 14 1.289034454109526 1101 15 1.2007357045291371 1101 16 1.3542447037802745 1101 17 1.3147097030676207 1101 18 0.8491256620478893 1101 19 1.0411696074675731 1101 20 1.0832427833676235 1101 21 1.1906754017327899 1101 22 1.183526643385811 1101 23 1.1171780380689285 1101 24 1.046444474896873 1101 25 1.1632520362632732 1102 1 0.5874408248812166 1102 2 0.5878972281239037 1102 3 0.5169385427781954 1102 4 0.483369146527572 1102 5 0.7363977485741131 1102 6 0.5906390481388399 1102 7 0.6488219360155014 1102 8 0.6795077233702358 1102 9 0.8804445606674278 1102 10 0.9602638906704541 1102 11 0.8236077944905063 1102 12 0.827237753316048 1102 13 0.8892641453844092 1102 14 1.0746941801664747 1102 15 1.003664681210843 1102 16 0.9560882845082048 1102 17 1.0461777100844216 1102 18 1.0477084318365897 1102 19 1.1076546042956252 1102 20 1.0249411480822417 1102 21 0.9707334984116258 1102 22 1.0107010952300328 1102 23 1.206929444897213 1102 24 1.1475938234281458 1102 25 1.194679206092033 1103 1 0.6334029975957556 1103 2 0.5339948893786612 1103 3 0.5804749031836849 1103 4 0.6748753789008717 1103 5 0.7629971056755167 1103 6 0.756364230391334 1103 7 0.7198985671790581 1103 8 0.7881112954494398 1103 9 0.8558489606104871 1103 10 0.9948350709339238 1103 11 0.9465342274983399 1103 12 0.9902623234311179 1103 13 0.8503356297753584 1103 14 1.1026980984831098 1103 15 0.8023008362431838 1103 16 0.8757538338838771 1103 17 0.9499459042242719 1103 18 1.0969511992273553 1103 19 1.0611472917969351 1103 20 1.079776874449145 1103 21 0.784056670934028 1103 22 1.1182724258150998 1103 23 1.0890036440364952 1103 24 1.1659207141702268 1103 25 1.1404217699015646 1104 1 0.5001403045658535 1104 2 0.512418087555961 1104 3 0.5244903599394384 1104 4 0.4745526643877227 1104 5 0.5640457240358074 1104 6 0.6566930785377139 1104 7 0.6733706888454947 1104 8 0.5955740248929402 1104 9 0.7216124562534745 1104 10 0.8182547578003039 1104 11 0.7897678789394931 1104 12 0.7247967360250982 1104 13 0.8078949639422959 1104 14 1.0334540377228763 1104 15 0.9918623241694391 1104 16 1.010590308504412 1104 17 0.9922060549020202 1104 18 1.0396223033318037 1104 19 0.9872420579832237 1104 20 1.0600624843632716 1104 21 0.851258387109084 1104 22 1.0086211151003823 1104 23 0.8809172250262733 1104 24 0.9046343437632274 1104 25 0.9965368275441264 1105 1 0.5037428583077563 1105 2 0.5829121799338601 1105 3 0.5160365303400951 1105 4 0.5524627892867073 1105 5 0.45432167823734915 1105 6 0.6745293433402324 1105 7 0.7093654384757571 1105 8 0.6699163996373088 1105 9 0.8291461707944521 1105 10 0.8077677867717981 1105 11 0.7862651464754293 1105 12 0.7112407387741939 1105 13 0.8633305767321175 1105 14 1.013533382252767 1105 15 0.8572940626698156 1105 16 0.9469980352067608 1105 17 0.6670948459669006 1105 18 0.8371071130957759 1105 19 0.9255435205370475 1105 20 1.0471124902339852 1105 21 0.9519126563045006 1105 22 0.8412512374904466 1105 23 0.9139789118987807 1105 24 0.9956971650032003 1105 25 1.138491098690082 1106 1 0.5047382983906594 1106 2 0.4634633585835424 1106 3 0.42606515707722536 1106 4 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-2.7334003010427494 2216 19 -2.8183432976011886 2216 20 -3.09921738807693 2216 21 -3.0204462523788465 2216 22 -3.1436368955817215 2216 23 -2.422248291174647 2216 24 -2.7971311515411514 2216 25 -2.570484187832051 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48845.0 3 2.0 4 763.0 5 6798.0 6 7625.0 7 5887.0 8 4957.0 9 4338.0 10 3830.0 11 3487.0 12 3227.0 13 3082.0 14 3004.0 15 3047.0 16 3289.0 17 3586.0 18 3870.0 19 4559.0 20 5697.0 21 7419.0 22 9480.0 23 11685.0 24 13837.0 25 16409.0 26 18971.0 27 20653.0 28 23671.0 29 29127.0 30 36531.0 31 46322.0 32 59582.0 33 76583.0 34 98333.0 35 128215.0 36 174452.0 37 268430.0 38 860030.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.69293312098439 19.232461638938744 13.229078194822558 27.845527045254308 2 14.153157524682861 20.51801824105525 39.19836404868243 26.130460185579462 3 16.969652256881606 24.50888111296019 29.989210857669974 28.53225577248823 4 11.79518979931164 17.255018694889205 37.17387955726619 33.77591194853296 5 13.161054267806538 37.705915552139835 35.85632377840575 13.27670640164787 6 32.25405404869714 37.1194079993816 17.69252953016343 12.934008421757826 7 27.825310362488725 31.39863357784559 22.484445344847483 18.291610714818198 8 25.222261532774592 36.40361960995343 20.4612911633512 17.91282769392077 9 26.204706169106217 14.846359401984206 19.73766213435242 39.21127229455716 10 14.641121428613918 28.64336404789806 34.786772710238125 21.928741813249893 11 34.519608901424185 22.112686935948844 24.452177770999565 18.915526391627406 12 22.92592941083252 24.59810131283768 31.567432117229323 20.908537159100476 13 28.815438483208244 21.593801947339728 25.65991226423924 23.930847305212797 14 21.250529851958373 21.35788677212206 27.37611367949007 30.01546969642949 15 23.28116396163704 30.003248412437454 23.18098807198799 23.534599553937515 16 22.819794075842292 27.697443034087925 26.70298486026575 22.779778029804028 17 22.208034457446775 27.94937378464294 27.3628324369946 22.479759320915683 18 22.223938728643112 27.260465655182248 28.545765857300882 21.96982975887376 19 23.694615454319027 26.795372607312856 27.10742402102658 22.40258791734153 20 23.807755328204312 26.902696672277 27.33533086835333 21.95421713116536 21 23.377542926349584 26.406079949614337 26.831951721658804 23.384425402377275 22 23.475219831067147 27.233998095680423 26.739894999997528 22.550887073254906 23 22.812475397636092 27.03356022386356 27.100305354019046 23.053659024481302 24 23.31281630284464 26.693744327530432 27.3357948488406 22.657644520784327 25 23.14290906408034 26.84845036723655 27.17109761960998 22.837542949073132 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 114.0 1 114.0 2 215.0 3 316.0 4 316.0 5 316.0 6 776.0 7 1236.0 8 1236.0 9 1236.0 10 1868.0 11 2500.0 12 2500.0 13 2500.0 14 4825.5 15 7151.0 16 7151.0 17 7151.0 18 13992.5 19 20834.0 20 20834.0 21 20834.0 22 33702.5 23 46571.0 24 46571.0 25 46571.0 26 67117.5 27 87664.0 28 87664.0 29 87664.0 30 112517.0 31 137370.0 32 137370.0 33 137370.0 34 159195.0 35 181020.0 36 181020.0 37 181020.0 38 199580.5 39 218141.0 40 218141.0 41 218141.0 42 232102.0 43 246063.0 44 246063.0 45 246063.0 46 255165.0 47 264267.0 48 264267.0 49 264267.0 50 258751.0 51 253235.0 52 253235.0 53 253235.0 54 230442.5 55 207650.0 56 207650.0 57 207650.0 58 179785.5 59 151921.0 60 151921.0 61 151921.0 62 125055.0 63 98189.0 64 98189.0 65 98189.0 66 76191.5 67 54194.0 68 54194.0 69 54194.0 70 39729.5 71 25265.0 72 25265.0 73 25265.0 74 17859.0 75 10453.0 76 10453.0 77 10453.0 78 7110.0 79 3767.0 80 3767.0 81 3767.0 82 2495.0 83 1223.0 84 1223.0 85 1223.0 86 806.5 87 390.0 88 390.0 89 390.0 90 232.5 91 75.0 92 75.0 93 75.0 94 44.0 95 13.0 96 13.0 97 13.0 98 7.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07729165294710943 2 1.4854257452999892E-4 3 0.0 4 0.030797827119219776 5 0.07437031564801945 6 0.07511302852066945 7 0.03832398422873972 8 0.07451885822254946 9 0.0535248410223096 10 0.11581369394188916 11 0.00401064951230997 12 0.01648822577282988 13 0.007427128726499946 14 0.009457210578409932 15 0.008664983514249935 16 0.021439644923829843 17 0.03312499412018976 18 8.417412556699939E-4 19 0.00415919208683997 20 0.0066349016623399515 21 4.951419150999964E-5 22 1.9805676603999855E-4 23 0.0 24 0.0 25 0.003961135320799971 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2019623.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.41464519239759 #Duplication Level Percentage of deduplicated Percentage of total 1 79.93911786519183 56.28884621470734 2 13.318957542022794 18.75699339308286 3 3.6881488252609356 7.790990728425203 4 1.349758724866693 3.8017112682732472 5 0.6046034326513786 2.128646809612624 6 0.34244434226636544 1.4467858115298051 7 0.19257275906032076 0.9491959752067488 8 0.11595674411188725 0.6532042395443343 9 0.0830382102906703 0.5262395503526323 >10 0.3377925164050834 3.9281681106515114 >50 0.01521818538480903 0.7369366713763364 >100 0.01049106274257474 1.529993473022297 >500 0.001196163913359916 0.6053476919766948 >1k 7.036258313880953E-4 0.8569400622383325 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2600 0.12873689792599907 No Hit GTCCTACAGTGGACATTTCTAAATT 2133 0.10561377049082923 No Hit CTGTAGGACGTGGAATATGGCAAGA 2024 0.10021672361623926 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 9.902838301999928E-5 0.0 8 0.0 0.0 0.0 9.902838301999928E-5 0.0 9 0.0 0.0 0.0 9.902838301999928E-5 0.0 10 0.0 0.0 0.0 9.902838301999928E-5 0.0 11 0.0 0.0 0.0 9.902838301999928E-5 0.0 12 0.0 0.0 0.0 9.902838301999928E-5 0.0 13 0.0 0.0 0.0 9.902838301999928E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCAA 50 0.0014970778 13.303228 10 TAGGACC 565 0.0 11.956066 4 GCACCGT 80 2.8335045E-5 11.888787 6 TGGCGAG 1155 0.0 11.338788 18 GGCGAGG 525 0.0 10.665033 19 TATGTCG 90 9.5970994E-5 10.546875 16 TAGGACT 255 0.0 10.447112 4 GGCGAGA 755 0.0 10.432795 19 GTAGGAC 1235 0.0 10.314285 3 CTAGGAC 270 0.0 10.210229 3 ACTAGAC 140 9.600626E-8 10.1850815 3 TGTAGGA 1345 0.0 10.106582 2 TATGGCG 1340 0.0 9.988048 16 AGGACCT 1120 0.0 9.939081 5 CTTACAC 240 0.0 9.902163 3 TTTATAC 270 0.0 9.858152 3 ATGGCGA 1375 0.0 9.802841 17 TTACACT 360 0.0 9.778622 4 TAGGACA 500 0.0 9.70462 4 GTCTAGA 265 0.0 9.685436 1 >>END_MODULE