FastQCFastQC Report
Thu 26 May 2016
SRR1512949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104567
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33870.3066359940139439No Hit
GGTATCAACGCAGAGTACTTTTTTT21360.1933789439662782No Hit
TATCAACGCAGAGTACTTTTTTTTT21100.19102508041612687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG707.014509E-613.6163251
ATTAGAC500.00150173613.2968623
AAAGGCG1001.445369E-712.3470865
GTATTAC853.7975133E-612.3347881
CGTGCGC550.00307916412.08477210
GTTCTAG1501.2732926E-1112.0731411
CTAGACT1251.8351784E-811.397314
TAGGACA1501.7644197E-1011.3973094
TAGTACT1001.933202E-611.3973094
TTAGACT855.3321928E-511.1738344
ACCGTGC855.3494376E-511.1702928
AAGGCGT855.3543736E-511.1692816
TAGACTG1553.1286618E-1011.02965455
GTATCAA13750.011.0217961
GTAGGAC2000.010.9224213
TTAGGCA803.777038E-410.6849784
GGTATCA6600.010.3979211
CAATACT1201.526334E-610.2892394
GACCGTG856.628824E-410.0523537
ATAGGAC951.6467605E-49.9976413
Produced by FastQC (version 0.11.5)