##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512949_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1104567
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.797145849912226	31.0	30.0	34.0	22.0	34.0
2	28.802145093959897	31.0	30.0	34.0	16.0	34.0
3	28.967589109578686	31.0	30.0	34.0	22.0	34.0
4	32.42217538637312	35.0	33.0	37.0	23.0	37.0
5	32.525015684879236	35.0	33.0	37.0	25.0	37.0
6	32.598208166639054	36.0	33.0	37.0	25.0	37.0
7	32.3877564692771	36.0	33.0	37.0	23.0	37.0
8	32.38144630429843	36.0	33.0	37.0	23.0	37.0
9	33.67016034337437	38.0	34.0	39.0	21.0	39.0
10	33.54934920199499	38.0	34.0	39.0	19.0	39.0
11	33.60707951622672	38.0	34.0	39.0	19.0	39.0
12	33.50773832642112	38.0	34.0	39.0	18.0	39.0
13	33.49193937533894	38.0	34.0	39.0	18.0	39.0
14	34.45350983688631	39.0	34.0	41.0	17.0	41.0
15	34.39303727161865	39.0	34.0	41.0	17.0	41.0
16	34.263477000489786	39.0	34.0	41.0	17.0	41.0
17	34.20307776712504	39.0	34.0	41.0	17.0	41.0
18	33.901605787607274	39.0	33.0	41.0	11.0	41.0
19	34.150801173672576	39.0	34.0	41.0	17.0	41.0
20	34.246434123054556	39.0	34.0	41.0	16.0	41.0
21	34.17995830040188	39.0	34.0	41.0	11.0	41.0
22	34.08331409502547	39.0	34.0	41.0	10.0	41.0
23	33.813982311620755	39.0	34.0	41.0	2.0	41.0
24	33.826535647000135	39.0	34.0	41.0	2.0	41.0
25	33.75184121922889	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6698194408483218
1101	2	1.0251901016880893
1101	3	1.121881710518469
1101	4	1.062038796494825
1101	5	0.9221395231332608
1101	6	1.1020420129663577
1101	7	1.115513136995709
1101	8	0.8882076693133456
1101	9	0.7342804371102218
1101	10	1.02052358551596
1101	11	1.0930007223897036
1101	12	1.3275977965220918
1101	13	1.0169074996104825
1101	14	1.1240857920359986
1101	15	0.7545361591885964
1101	16	1.2898408739391343
1101	17	1.4316572550829747
1101	18	1.4659153133911502
1101	19	1.0496043846291059
1101	20	1.2477473157996144
1101	21	1.3089091584408976
1101	22	1.274194881154834
1101	23	1.4702773848904371
1101	24	1.1662431088685494
1101	25	1.3397050663531047
1102	1	0.6079218487442795
1102	2	0.6948369879696763
1102	3	0.636164490764699
1102	4	0.7345068030670774
1102	5	0.7028413536433078
1102	6	0.6799023295450795
1102	7	0.8606678393931873
1102	8	0.8361778512072675
1102	9	0.9825621644081934
1102	10	1.2007006775274434
1102	11	1.0837187231765242
1102	12	1.1294453735840193
1102	13	0.9309157612044814
1102	14	1.0914820014222784
1102	15	1.2200672905872807
1102	16	1.2897454143445728
1102	17	1.0877163358938589
1102	18	1.5297307755312914
1102	19	1.2915026131304472
1102	20	1.262140742394081
1102	21	1.1682465531460693
1102	22	1.5093024612251824
1102	23	1.7030722393290176
1102	24	1.564677716153561
1102	25	1.501826554597109
1103	1	0.31024081976141105
1103	2	0.5031530424720465
1103	3	0.42221648675448975
1103	4	0.44167618877615666
1103	5	0.20658383450368234
1103	6	0.6395268282691049
1103	7	0.46811178762504824
1103	8	0.3043753083709575
1103	9	0.7300329992605441
1103	10	0.5333143760327275
1103	11	0.5356617372122514
1103	12	0.7900188788702067
1103	13	0.7816152690426179
1103	14	0.9455993000004739
1103	15	1.0734753975869182
1103	16	1.2210819983766612
1103	17	0.9072911627257909
1103	18	1.115474600722692
1103	19	1.1424613034330378
1103	20	0.9831493324414708
1103	21	0.6828348232882462
1103	22	0.9453128740943058
1103	23	1.2095062303971957
1103	24	0.9742629102840752
1103	25	0.9818922901056766
1104	1	0.5361946215815152
1104	2	0.5540436347372939
1104	3	0.6271700180708208
1104	4	0.767417934960811
1104	5	0.5208091586251626
1104	6	0.5542191164273902
1104	7	0.7979704356030339
1104	8	0.7333535769812016
1104	9	0.8259355571773384
1104	10	0.9188367657403376
1104	11	0.9283272896609986
1104	12	0.7379327565417739
1104	13	0.8398650130301206
1104	14	1.0003528517426048
1104	15	1.1553541980959068
1104	16	0.9713387009356751
1104	17	0.9213273412251581
1104	18	1.2461689811448409
1104	19	1.119938069091205
1104	20	1.154644928474454
1104	21	1.1963751932159568
1104	22	0.9897003348643594
1104	23	1.2435403663170561
1104	24	1.1178258865984319
1104	25	0.8607762964321566
1105	1	0.47038586760757894
1105	2	0.6305772528799238
1105	3	0.7515358829075858
1105	4	0.904580658351918
1105	5	0.6090850900024876
1105	6	0.4599956438740662
1105	7	0.5714866781976937
1105	8	0.5901343843238323
1105	9	0.4795472652945705
1105	10	0.7150673816831556
1105	11	0.6940600637626915
1105	12	0.7322978356124352
1105	13	0.7711442031447078
1105	14	0.6627580581680803
1105	15	0.7796801001503297
1105	16	0.7544254397331969
1105	17	0.7276474636832688
1105	18	0.7320648168880268
1105	19	0.6363769366572427
1105	20	0.6275368411201967
1105	21	0.6971351605577638
1105	22	0.44884336419164583
1105	23	0.6778892714211295
1105	24	0.609117203815579
1105	25	0.6312685198770538
1106	1	0.6180748995786765
1106	2	0.6785546110377929
1106	3	0.6851777717739012
1106	4	0.6418541512683902
1106	5	0.7053298228872862
1106	6	0.6840578048654109
1106	7	0.4995088745743814
1106	8	0.7098741052251469
1106	9	0.704792030992671
1106	10	0.848585375471167
1106	11	0.8414706798274878
1106	12	0.6880217517708047
1106	13	0.8552957422709255
1106	14	0.8989913030822692
1106	15	1.0303584903363046
1106	16	0.9250721833111584
1106	17	0.9376665252294245
1106	18	1.1977581769006704
1106	19	0.7379516769101144
1106	20	0.8650358840655201
1106	21	0.7581596184007893
1106	22	0.785819416114883
1106	23	1.011921406582772
1106	24	0.9703618422378355
1106	25	0.908240640832858
1107	1	0.5953002669301384
1107	2	0.6563705862355711
1107	3	0.773941002547712
1107	4	0.7762295131604517
1107	5	0.8412070161599843
1107	6	0.7517676029999549
1107	7	0.9969422947066633
1107	8	0.8415955368453254
1107	9	1.0081457571160684
1107	10	0.7758410176786228
1107	11	0.8368513654619818
1107	12	0.8786547444154991
1107	13	0.9382551342591725
1107	14	1.1410846025783457
1107	15	1.2064952863631788
1107	16	1.242210398782106
1107	17	1.1959338770232506
1107	18	1.4152931087905216
1107	19	1.3846530410984386
1107	20	1.3826158146484175
1107	21	1.3076793566142797
1107	22	1.187169707779205
1107	23	1.197214774900381
1107	24	1.2759750983234142
1107	25	1.3681358865224098
1108	1	0.887651769558758
1108	2	0.8931189449832502
1108	3	0.7596797622579103
1108	4	0.8970492238815524
1108	5	0.8976235934623134
1108	6	1.0136127367849
1108	7	0.9026644457280284
1108	8	1.020213484691432
1108	9	1.1021888612407764
1108	10	0.8158731888396602
1108	11	0.7883638023620421
1108	12	0.7713429980184117
1108	13	0.9672562589420366
1108	14	1.1261256665806485
1108	15	1.0441976638904507
1108	16	1.083721658688468
1108	17	1.0307310417762139
1108	18	1.2210132981557251
1108	19	1.2597223529494528
1108	20	1.149548830629378
1108	21	1.1413093359514974
1108	22	1.0522535358858178
1108	23	1.2449627936276642
1108	24	1.3035422523526563
1108	25	1.439149801203449
1109	1	0.10450904881485101
1109	2	-0.002339044140416746
1109	3	0.28038423317824623
1109	4	0.36620008111929536
1109	5	0.1627368194160681
1109	6	0.073339167936048
1109	7	0.30326222894859
1109	8	0.32703661973231846
1109	9	0.5816270903079825
1109	10	0.6572219372310428
1109	11	0.16452875316946347
1109	12	0.3397819120455594
1109	13	0.554349953159317
1109	14	0.5143604417892504
1109	15	0.8121026205096555
1109	16	0.8182166564052693
1109	17	0.48783542551982606
1109	18	-0.8662256905943266
1109	19	0.3605360023329922
1109	20	0.6840874167060065
1109	21	0.6451674247057042
1109	22	0.6835868207876388
1109	23	0.3602981126529414
1109	24	0.6294327002749469
1109	25	0.7471456706874449
1110	1	0.37791936200687815
1110	2	0.44839213918505294
1110	3	0.5874047340573014
1110	4	0.4378397106202314
1110	5	0.5712066567263179
1110	6	0.5717632244444175
1110	7	0.7029366679360436
1110	8	0.6824968004180363
1110	9	0.7659551069659187
1110	10	1.0270727314910033
1110	11	0.8418301752137438
1110	12	0.36133363618348824
1110	13	0.571837674932361
1110	14	0.7085855910209204
1110	15	0.8206432488644495
1110	16	1.1185586834666381
1110	17	0.5630894823912271
1110	18	1.1265188756095625
1110	19	0.8971159132348134
1110	20	0.874010972750213
1110	21	0.9212054988781233
1110	22	0.8684386393136307
1110	23	0.6881005174739983
1110	24	0.7935706831709979
1110	25	0.9947097975201018
1111	1	0.28090007504019
1111	2	0.6103240934635394
1111	3	0.5749970729253775
1111	4	0.5308732160958911
1111	5	0.47541376406456237
1111	6	0.588137504656352
1111	7	0.6222150115372358
1111	8	0.43399865438129837
1111	9	0.7108727565045854
1111	10	0.6143048170320071
1111	11	0.508308411505439
1111	12	0.7166813860373793
1111	13	0.7295251551711246
1111	14	0.6847571329243252
1111	15	0.7780739527956158
1111	16	0.7022361417644376
1111	17	0.6874822734922006
1111	18	0.9201109653662982
1111	19	0.7154179319948142
1111	20	0.5483266351468572
1111	21	0.7489833298848154
1111	22	0.6803116691782449
1111	23	1.1281269616011969
1111	24	1.0603880941376431
1111	25	0.7391023969969694
1112	1	-0.5628390243718471
1112	2	-0.42769450976531687
1112	3	-0.33289721975015496
1112	4	-0.17164655306150678
1112	5	-0.1998491572896981
1112	6	-0.2472799162951489
1112	7	-0.5748911160907397
1112	8	-0.3201932939273249
1112	9	-0.2242012731734775
1112	10	-0.18425364122854404
1112	11	-0.35725569205999363
1112	12	-0.18753971875851505
1112	13	-0.4546061701484234
1112	14	-0.48018419336820983
1112	15	-0.5736191422465922
1112	16	-0.281653988913547
1112	17	-0.21416372770053016
1112	18	-0.1644219980116972
1112	19	-0.29186225423604384
1112	20	-0.39526830347228525
1112	21	-0.3696038149173475
1112	22	-0.3184502556926674
1112	23	-0.2447434730588185
1112	24	-0.4109088168632553
1112	25	-0.21123030466763026
1113	1	-0.22383433257440188
1113	2	0.053113773683961085
1113	3	-0.13198798176277649
1113	4	0.02856312174839104
1113	5	-0.19050892108196393
1113	6	-0.0890317363889821
1113	7	-0.20377446829911605
1113	8	-0.0034581378037188415
1113	9	0.013820406166438204
1113	10	-0.2749930037912378
1113	11	-0.2833369086916093
1113	12	-0.060360945097301055
1113	13	-0.10764742560870388
1113	14	-0.011410855720576762
1113	15	-0.3235625171050245
1113	16	-0.35129579444663506
1113	17	-0.37004465311713375
1113	18	-0.4259059002928822
1113	19	-0.5516409308255916
1113	20	-0.49463604332020594
1113	21	-0.4560927325682158
1113	22	-0.2140127860275598
1113	23	-0.7598322936380129
1113	24	-0.20930648714314515
1113	25	-0.0826452860622311
1114	1	-1.6306817351718053
1114	2	-1.2803050574848704
1114	3	-1.194845124619917
1114	4	-1.5212842808490095
1114	5	-1.452119310692357
1114	6	-1.9801064117136598
1114	7	-1.9294108319354812
1114	8	-1.6113964494920339
1114	9	-1.6123679055218716
1114	10	-1.8244085882068255
1114	11	-2.452918077522778
1114	12	-2.07612717886353
1114	13	-1.92083249054377
1114	14	-2.142608907668631
1114	15	-2.137198880741387
1114	16	-2.9003769632778003
1114	17	-4.582869328441806
1114	18	-3.7237110950980785
1114	19	-2.9064104113450746
1114	20	-2.0519217576059177
1114	21	-3.021242083217569
1114	22	-3.0975485003132768
1114	23	-2.8739491767548984
1114	24	-2.603209084545739
1114	25	-2.3553303509972956
1115	1	-1.0941674225395204
1115	2	-0.8178111877827376
1115	3	-0.9352199116727284
1115	4	-0.8466240284097957
1115	5	-0.6935059195406339
1115	6	-0.7578679495795413
1115	7	-1.921736989593839
1115	8	-1.4221796434417513
1115	9	-1.0962310256498995
1115	10	-0.8310796428761762
1115	11	-1.572836705884761
1115	12	-1.098534385512643
1115	13	-0.9109559353839956
1115	14	-0.991194744744675
1115	15	-2.157236565940913
1115	16	-1.1703310828379685
1115	17	-1.1618837056035431
1115	18	-1.1463209242240922
1115	19	-1.2773840962348615
1115	20	-0.904974000649645
1115	21	-1.6959729954058886
1115	22	-1.696082713828325
1115	23	-1.1107296733735552
1115	24	-1.2683044590746775
1115	25	-1.4203047328051497
1116	1	-1.1908157787071048
1116	2	-2.0493380855204357
1116	3	-1.4876297317818619
1116	4	-1.0923616701886907
1116	5	-1.7610887485233668
1116	6	-1.4156091205227526
1116	7	-1.1405205933659275
1116	8	-1.807869835138991
1116	9	-1.8487163579780237
1116	10	-2.6747547812975405
1116	11	-1.3522308538698908
1116	12	-1.436874955939473
1116	13	-1.7222327504922639
1116	14	-1.6610066410149216
1116	15	-1.518164334315074
1116	16	-1.4755783018910833
1116	17	-1.625370300211884
1116	18	-1.292372724756497
1116	19	-1.7442496198383424
1116	20	-2.0919939115343027
1116	21	-2.5131320545503506
1116	22	-1.7261328135147735
1116	23	-1.8583000343419904
1116	24	-2.324672448813754
1116	25	-2.0761253196577094
1201	1	0.4217799271815892
1201	2	0.3101033038388472
1201	3	0.6859543908252661
1201	4	0.6868557582457555
1201	5	0.690774803067896
1201	6	0.6428793131913579
1201	7	0.6405550289752568
1201	8	0.09485630366032893
1201	9	0.5863250353954754
1201	10	0.575653357721265
1201	11	0.21592552965744716
1201	12	-0.23842826360556302
1201	13	-0.08969674636668401
1201	14	0.6285618015434196
1201	15	0.11913887599006756
1201	16	0.6540889939932768
1201	17	0.8327761481288647
1201	18	1.065831055664546
1201	19	0.8549632051958653
1201	20	0.6582690358525909
1201	21	0.4444901176524283
1201	22	0.8413029533511676
1201	23	1.1241002147984318
1201	24	1.0572343106417463
1201	25	0.9043714137733687
1202	1	0.8920502893747333
1202	2	0.8518057006339532
1202	3	0.5111285910312162
1202	4	0.9664119014036245
1202	5	0.9117016950641315
1202	6	0.8704753467310056
1202	7	0.7323736798490259
1202	8	0.7821947039781492
1202	9	1.2150718142565538
1202	10	1.4368247125426024
1202	11	1.0448098740358205
1202	12	1.2754151259045798
1202	13	1.2800248734184336
1202	14	1.2850677242418698
1202	15	1.0824111985461116
1202	16	1.0664519440752969
1202	17	1.4137921791067996
1202	18	1.629077543980884
1202	19	1.4063020628593321
1202	20	1.2917759723821334
1202	21	1.3785773476502499
1202	22	1.5007863264489671
1202	23	1.5683800292839862
1202	24	1.6130597869650671
1202	25	1.454173966369929
1203	1	0.6166430336807416
1203	2	0.8721637442894874
1203	3	0.8852254221172942
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2211	17	0.47321734803960425
2211	18	0.35419126569902204
2211	19	0.15884374239592347
2211	20	-0.05077947883206235
2211	21	0.10382166881986876
2211	22	0.016148000554274233
2211	23	0.5749138023712419
2211	24	0.35007118781347657
2211	25	0.2965010412946256
2212	1	-0.2248699449939302
2212	2	-0.198252537130152
2212	3	-0.2635273836045151
2212	4	-0.3869642191366225
2212	5	-0.24528946056530287
2212	6	-0.41338280802128935
2212	7	-0.5635211882078472
2212	8	-0.33499916950447783
2212	9	-0.06497447607625162
2212	10	-0.1551364313847543
2212	11	-0.4687555379801722
2212	12	-0.3190172893235257
2212	13	-0.3291315752297592
2212	14	-0.35851443326112076
2212	15	-0.5091301155077659
2212	16	-0.3335062092457548
2212	17	-0.5369422164778967
2212	18	-3.399973865413898
2212	19	-1.5219308827447549
2212	20	-0.7914682493343861
2212	21	-0.9263145563804542
2212	22	-0.5737999264399178
2212	23	-0.4667653879253564
2212	24	-0.8806587811697071
2212	25	-0.7472646324887648
2213	1	-0.17244049246955484
2213	2	-0.07688032854408533
2213	3	-0.553904757647441
2213	4	-0.4612081757614348
2213	5	-0.5998778594830156
2213	6	-0.5069360614217473
2213	7	-0.3481139178404007
2213	8	-0.6930321876071304
2213	9	-0.5829371298755035
2213	10	-0.5537872370808827
2213	11	-0.6141409716011736
2213	12	-0.6791401063030662
2213	13	-0.44966380830857133
2213	14	-0.5664881820639636
2213	15	-0.7951221501325492
2213	16	-1.2333888481818889
2213	17	-0.9066599873242112
2213	18	-0.6334275254567103
2213	19	-0.582697942621138
2213	20	-1.327380473202247
2213	21	-1.072722483551182
2213	22	-1.01939483058851
2213	23	-1.312867024961733
2213	24	-1.0609342722021182
2213	25	-1.12498735683549
2214	1	-1.6004744837641631
2214	2	-3.6241507224933436
2214	3	-1.8366971991839591
2214	4	-1.4662273601689684
2214	5	-1.490040920051129
2214	6	-1.467843865931119
2214	7	-1.4512525200128898
2214	8	-1.2244133821985344
2214	9	-1.4157072976390808
2214	10	-1.6485038705552633
2214	11	-1.2555997727067307
2214	12	-1.8118266267397694
2214	13	-1.463877302767024
2214	14	-2.8516366095217975
2214	15	-3.107867989143511
2214	16	-2.1157991876259103
2214	17	-2.6324791518715323
2214	18	-2.0177921643595447
2214	19	-4.031117608086998
2214	20	-2.401996922969719
2214	21	-2.3600813933361913
2214	22	-2.3838871954425613
2214	23	-2.5034536320110057
2214	24	-2.575790809795837
2214	25	-2.4066057705566877
2215	1	-1.4576814314371909
2215	2	-3.148182150612545
2215	3	-1.5851995697099817
2215	4	-1.2789783408564048
2215	5	-1.2242338318481316
2215	6	-1.7907486783662385
2215	7	-2.340818047613471
2215	8	-2.4151274330639545
2215	9	-2.2804473783442276
2215	10	-3.7046169765645445
2215	11	-2.6900526055455956
2215	12	-2.7943697439683852
2215	13	-2.122059644418364
2215	14	-3.476120409966448
2215	15	-3.4090360789876506
2215	16	-3.048442635983946
2215	17	-3.426490241230354
2215	18	-2.1761105985580116
2215	19	-2.736682517942718
2215	20	-3.160710252666931
2215	21	-2.905121038370492
2215	22	-2.334776129598577
2215	23	-2.8722944833948
2215	24	-2.7798010501048758
2215	25	-2.671207417713262
2216	1	-2.2082482603322937
2216	2	-2.0387371862900032
2216	3	-2.6019066280367724
2216	4	-3.325559234255511
2216	5	-2.621067333057237
2216	6	-2.6517736823701092
2216	7	-2.5097380218298717
2216	8	-2.9934255963133225
2216	9	-3.4384887379923654
2216	10	-2.6769194600229334
2216	11	-3.2873674976925855
2216	12	-3.9082249216674256
2216	13	-4.345986507938672
2216	14	-3.6053431694205464
2216	15	-3.479371173141473
2216	16	-2.6546156772972687
2216	17	-2.850194343972973
2216	18	-2.880394673478694
2216	19	-3.4131651742359423
2216	20	-3.0867167462163962
2216	21	-3.430237216785592
2216	22	-3.567893033342891
2216	23	-3.5807362962413336
2216	24	-3.5468697803419715
2216	25	-3.7928704731757996
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60252.0
3	2.0
4	478.0
5	2837.0
6	3557.0
7	3280.0
8	2959.0
9	2850.0
10	2639.0
11	2385.0
12	2269.0
13	2297.0
14	2235.0
15	2241.0
16	2204.0
17	2411.0
18	2717.0
19	3250.0
20	4116.0
21	5206.0
22	6688.0
23	8138.0
24	9265.0
25	10580.0
26	11793.0
27	12808.0
28	14462.0
29	17138.0
30	21012.0
31	26182.0
32	33302.0
33	42421.0
34	54532.0
35	72042.0
36	99209.0
37	155261.0
38	399549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62367140891985	18.538490753242318	12.612188602237683	25.225649235600155
2	15.55950848788564	20.582681144784498	39.44659148648269	24.41121888084717
3	18.61980334140291	24.667548795586093	29.852546807626158	26.86010105538483
4	12.631344427156257	16.75955796181344	37.78082484571954	32.828272765310764
5	13.544560266427796	37.42723639126918	35.444244078149495	13.583959264153526
6	31.9981527929951	36.88600740693789	17.841665383884024	13.274174416182982
7	27.507176037016578	31.855809194382317	22.35826760958737	18.278747159013736
8	25.50268900699169	35.21435537633873	20.863325639244742	18.419629977424837
9	26.354922017052658	15.256659156929361	20.044388342762762	38.34403048325522
10	15.020319755438521	28.575697427613132	34.304709402142805	22.09927341480553
11	33.94973916996194	22.969251846546275	24.245376729849625	18.835632253642157
12	22.554122432421664	25.206003904566316	31.31105412424344	20.928819538768575
13	28.38269423886833	21.973488453378415	25.887287442647207	23.75652986510605
14	21.560113233586836	21.524976771900974	27.709718491740215	29.205191502771978
15	23.52029732155228	29.305262624076416	23.845855003219356	23.328585051151947
16	23.062351212170878	28.161591392513724	26.13785053710765	22.638206858207745
17	22.24845244692681	27.9413469235478	27.27161593880057	22.53858469072482
18	22.163664195749565	27.558142380291635	28.30258961417259	21.97560380978621
19	23.22849155889845	27.388958968540223	27.623949673050507	21.758599799510822
20	23.346206959002107	27.06087428972449	27.756774500260338	21.836144251013064
21	23.435393154134747	26.823914435287204	27.385362681983942	22.355329728594107
22	23.256986902726666	26.97636260902321	27.206180638057432	22.560469850192693
23	22.9997301521471	26.925020329141937	27.286688894402563	22.788560624308403
24	22.895647591261497	26.868015147316893	27.637333681047927	22.599003580373683
25	23.054437975914613	26.806879521443495	27.46230489142197	22.676377611219927
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	439.0
1	439.0
2	566.5
3	694.0
4	694.0
5	694.0
6	1287.5
7	1881.0
8	1881.0
9	1881.0
10	2617.5
11	3354.0
12	3354.0
13	3354.0
14	4952.5
15	6551.0
16	6551.0
17	6551.0
18	10790.5
19	15030.0
20	15030.0
21	15030.0
22	21717.0
23	28404.0
24	28404.0
25	28404.0
26	38893.0
27	49382.0
28	49382.0
29	49382.0
30	61706.5
31	74031.0
32	74031.0
33	74031.0
34	84348.5
35	94666.0
36	94666.0
37	94666.0
38	104343.5
39	114021.0
40	114021.0
41	114021.0
42	121541.5
43	129062.0
44	129062.0
45	129062.0
46	134296.0
47	139530.0
48	139530.0
49	139530.0
50	137666.5
51	135803.0
52	135803.0
53	135803.0
54	125510.5
55	115218.0
56	115218.0
57	115218.0
58	100334.5
59	85451.0
60	85451.0
61	85451.0
62	70531.5
63	55612.0
64	55612.0
65	55612.0
66	43467.0
67	31322.0
68	31322.0
69	31322.0
70	22984.5
71	14647.0
72	14647.0
73	14647.0
74	10359.5
75	6072.0
76	6072.0
77	6072.0
78	4208.0
79	2344.0
80	2344.0
81	2344.0
82	1539.0
83	734.0
84	734.0
85	734.0
86	479.5
87	225.0
88	225.0
89	225.0
90	145.0
91	65.0
92	65.0
93	65.0
94	42.0
95	19.0
96	19.0
97	19.0
98	14.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3589641913980773
2	0.042007411048854436
3	0.038023949656290655
4	0.03702808430814971
5	0.043274876037397464
6	0.017835043053069665
7	0.017835043053069665
8	0.023176502647643826
9	0.02018890660322099
10	0.02018890660322099
11	0.01883090840121061
12	0.019826773749351555
13	0.019736240535884197
14	0.027250497253674972
15	0.02788422974794648
16	0.020460506243623066
17	0.021999570872568163
18	0.10845878973389572
19	0.024262901209252133
20	0.021909037659100808
21	0.02534929977086044
22	0.01946464089548212
23	0.021818504445633446
24	0.019283574468547404
25	0.02227117051297024
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1104567.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.1453880554345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.80969728565586	63.8172192262476
2	11.3611007951572	17.301908575683004
3	2.70967871963592	6.189886128366901
4	0.9317691312783271	2.837996883170532
5	0.4095008378239118	1.5590800102563656
6	0.21769950457256657	0.9946087953092351
7	0.13353872447625753	0.7117850596970664
8	0.09318761940441041	0.5676645953208774
9	0.0647621320661809	0.44382039097289777
>10	0.2513965858371165	3.1191043619743533
>50	0.011229455842043445	0.5783845952025347
>100	0.0056044988705893604	0.9065385473584584
>500	4.769767883374091E-4	0.2789343946858511
>1k	3.577325912530568E-4	0.6930684357542646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3387	0.3066359940139439	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2136	0.1933789439662782	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2110	0.19102508041612687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.053321346735871E-5
2	0.0	0.0	0.0	0.0	9.053321346735871E-5
3	0.0	0.0	0.0	0.0	9.053321346735871E-5
4	0.0	0.0	0.0	0.0	9.053321346735871E-5
5	0.0	9.053321346735871E-5	0.0	0.0	9.053321346735871E-5
6	0.0	9.053321346735871E-5	0.0	0.0	9.053321346735871E-5
7	0.0	9.053321346735871E-5	0.0	0.0	9.053321346735871E-5
8	0.0	9.053321346735871E-5	0.0	9.053321346735871E-5	9.053321346735871E-5
9	0.0	9.053321346735871E-5	0.0	9.053321346735871E-5	9.053321346735871E-5
10	0.0	9.053321346735871E-5	0.0	9.053321346735871E-5	9.053321346735871E-5
11	0.0	9.053321346735871E-5	0.0	1.8106642693471741E-4	9.053321346735871E-5
12	0.0	9.053321346735871E-5	0.0	1.8106642693471741E-4	9.053321346735871E-5
13	0.0	9.053321346735871E-5	0.0	1.8106642693471741E-4	9.053321346735871E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	70	7.014509E-6	13.616325	1
ATTAGAC	50	0.001501736	13.296862	3
AAAGGCG	100	1.445369E-7	12.347086	5
GTATTAC	85	3.7975133E-6	12.334788	1
CGTGCGC	55	0.003079164	12.084772	10
GTTCTAG	150	1.2732926E-11	12.073141	1
CTAGACT	125	1.8351784E-8	11.39731	4
TAGGACA	150	1.7644197E-10	11.397309	4
TAGTACT	100	1.933202E-6	11.397309	4
TTAGACT	85	5.3321928E-5	11.173834	4
ACCGTGC	85	5.3494376E-5	11.170292	8
AAGGCGT	85	5.3543736E-5	11.169281	6
TAGACTG	155	3.1286618E-10	11.0296545	5
GTATCAA	1375	0.0	11.021796	1
GTAGGAC	200	0.0	10.922421	3
TTAGGCA	80	3.777038E-4	10.684978	4
GGTATCA	660	0.0	10.397921	1
CAATACT	120	1.526334E-6	10.289239	4
GACCGTG	85	6.628824E-4	10.052353	7
ATAGGAC	95	1.6467605E-4	9.997641	3
>>END_MODULE