FastQCFastQC Report
Thu 26 May 2016
SRR1512949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104567
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44660.4043213313452239No Hit
TATCAACGCAGAGTACTTTTTTTTT32260.2920601466456992No Hit
GGTATCAACGCAGAGTACTTTTTTT28490.25792912516850497No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14660.13272169094314787No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11480.10393212906052779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC1501.4551915E-1112.04025651
ACTACAC1052.6887756E-711.7713413
GGTATCA7100.011.1120231
AATCCCG600.005934179811.06812319
GACTGCG951.348419E-511.0078267
TCTACAC2000.010.9337653
TCCAACG700.00150798610.84224318
CTAGGAC1605.347829E-1010.69607453
TTGGACC909.349284E-510.5731384
GTCTAGA1002.3814742E-510.4560131
ATCTCCG750.002630958810.1405437
CTGCGCA856.560426E-410.0636989
TAGGACT951.6154755E-410.0166574
CCCTTAA1151.0182874E-59.9187471
GTATTAC1252.5476747E-69.8856851
CTTGGAC1356.3623156E-79.85975553
CCAGTAC1752.5302143E-99.7792683
CTACACT2054.0017767E-119.7479174
TACACAG2950.09.677115
TGGGTTG900.00109615649.5158245
Produced by FastQC (version 0.11.5)