##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512949_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1104567
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.132837573456385	31.0	30.0	34.0	26.0	34.0
2	30.28005544253993	31.0	30.0	34.0	26.0	34.0
3	30.391839517204478	31.0	31.0	34.0	26.0	34.0
4	33.84760000977759	37.0	35.0	37.0	30.0	37.0
5	33.45223693990496	37.0	35.0	37.0	28.0	37.0
6	33.29663569525434	37.0	35.0	37.0	27.0	37.0
7	33.150130322560784	37.0	35.0	37.0	26.0	37.0
8	33.02595496696896	37.0	35.0	37.0	26.0	37.0
9	34.42471303234661	39.0	35.0	39.0	26.0	39.0
10	34.320787240611025	39.0	35.0	39.0	26.0	39.0
11	34.339259637486904	39.0	35.0	39.0	26.0	39.0
12	34.18250590502885	39.0	35.0	39.0	25.0	39.0
13	34.15801214412525	39.0	35.0	39.0	25.0	39.0
14	35.187310502667565	40.0	35.0	41.0	25.0	41.0
15	35.188616896937894	40.0	35.0	41.0	25.0	41.0
16	35.15940454494838	40.0	35.0	41.0	25.0	41.0
17	35.1474595927635	40.0	35.0	41.0	25.0	41.0
18	35.07433138958524	40.0	35.0	41.0	24.0	41.0
19	35.052636915642054	40.0	35.0	41.0	24.0	41.0
20	35.02281980178658	40.0	35.0	41.0	23.0	41.0
21	34.97633552333177	40.0	35.0	41.0	23.0	41.0
22	34.93112233119403	40.0	35.0	41.0	22.0	41.0
23	34.82245259907276	40.0	35.0	41.0	21.0	41.0
24	34.79446516146146	40.0	35.0	41.0	19.0	41.0
25	34.7055769364828	40.0	35.0	41.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6442939101089564
1101	2	0.6605657679747203
1101	3	0.652342621772096
1101	4	0.3991179573055632
1101	5	0.693259363139191
1101	6	0.7430700002814206
1101	7	0.7856206189575872
1101	8	0.8984192501198294
1101	9	0.7895762760409752
1101	10	1.1099891080199455
1101	11	1.027877230953564
1101	12	1.1031811813743317
1101	13	1.158240428699287
1101	14	1.3607342931168347
1101	15	1.2536507503110883
1101	16	1.3762424688887975
1101	17	1.3538791806401207
1101	18	0.7824791955519785
1101	19	1.1015838801991933
1101	20	1.1973381899559215
1101	21	1.3860538343368134
1101	22	1.1245737094455492
1101	23	1.1316602627045995
1101	24	1.226282038425751
1101	25	1.232027972140024
1102	1	0.6253082984732998
1102	2	0.5272094375572429
1102	3	0.5507056343611936
1102	4	0.5310199752635398
1102	5	0.7423595841426192
1102	6	0.813919098911434
1102	7	0.7412217829861021
1102	8	0.8570527026330197
1102	9	0.900505387167513
1102	10	0.8486279699101615
1102	11	0.9234907179479137
1102	12	1.063188673506147
1102	13	1.0118415695860747
1102	14	1.152745916045653
1102	15	1.1671809907535575
1102	16	0.9915996034810419
1102	17	1.1303120870087184
1102	18	1.0969527503515693
1102	19	1.1506768694151503
1102	20	1.254154005007294
1102	21	1.3134987570434689
1102	22	1.2218484826566893
1102	23	1.1665558067664392
1102	24	1.044768512090478
1102	25	1.0385950137025546
1103	1	0.4513455979629093
1103	2	0.38404672793274486
1103	3	0.47307051770060227
1103	4	0.402852822511079
1103	5	0.502581626614365
1103	6	0.5165342784593747
1103	7	0.6023056783561316
1103	8	0.5661673809049859
1103	9	0.6370991029896089
1103	10	0.6557449494945615
1103	11	0.49820362352574676
1103	12	0.7097463885991075
1103	13	0.8069962815802469
1103	14	0.8677221054368331
1103	15	0.6846259643226418
1103	16	0.8048002505827583
1103	17	0.8371710233922443
1103	18	0.7971071205666647
1103	19	0.732495931573375
1103	20	0.9378830425574236
1103	21	0.4242579622931899
1103	22	0.8083698552414518
1103	23	0.9461510264164588
1103	24	0.8995636724987861
1103	25	0.9065885426854194
1104	1	0.18548110379116167
1104	2	0.32370748031966556
1104	3	0.33236094214310086
1104	4	0.3337925884977082
1104	5	0.4823252414832382
1104	6	0.5672137121680052
1104	7	0.6885380714588365
1104	8	0.6611255908324765
1104	9	0.6909252132281765
1104	10	0.859036469753363
1104	11	0.8308901400168196
1104	12	0.7799096845495583
1104	13	0.9504874915877792
1104	14	0.9716555891113785
1104	15	0.9022493540661287
1104	16	1.0467389621567307
1104	17	0.9217707330147249
1104	18	0.8324558220105445
1104	19	0.9923789800956797
1104	20	1.1218528648253994
1104	21	0.7983308658199562
1104	22	1.011174537716947
1104	23	0.9347005051446473
1104	24	0.9897887564791645
1104	25	1.16767609205926
1105	1	0.42093439809024247
1105	2	0.507172398561206
1105	3	0.4699290264149987
1105	4	0.42706302457091994
1105	5	0.30955348697545304
1105	6	0.5250730424996917
1105	7	0.6772888011590084
1105	8	0.6421350068733673
1105	9	0.6804542897776429
1105	10	0.8451307704535722
1105	11	0.626184918651667
1105	12	0.7390845829311132
1105	13	0.7629015168044617
1105	14	0.8659390227001467
1105	15	0.7540649964771333
1105	16	0.6837200825242888
1105	17	0.6231273430106796
1105	18	0.7675572752056965
1105	19	0.8404077694864185
1105	20	0.7075288546799143
1105	21	0.653697968659877
1105	22	0.7566638862021904
1105	23	0.6135079887405155
1105	24	0.8462869578167229
1105	25	0.7277694936811798
1106	1	0.5116880401183153
1106	2	0.5747273666444705
1106	3	0.5096593076912974
1106	4	0.5336976424732569
1106	5	0.5251813032434924
1106	6	0.552141697542055
1106	7	0.4445297890184179
1106	8	0.43079346977228994
1106	9	0.4062945009229608
1106	10	0.6712600369701818
1106	11	0.29020453504095656
1106	12	0.44074834998450285
1106	13	0.37757224524985133
1106	14	0.6243645012026278
1106	15	0.5469298596937193
1106	16	0.6976829716726698
1106	17	0.6199553903639199
1106	18	0.7229885232149442
1106	19	0.8313241190959246
1106	20	0.9806893292994872
1106	21	0.744900390613104
1106	22	0.8230693943294511
1106	23	0.908885721235194
1106	24	0.8137825636513938
1106	25	0.7663274451234514
1107	1	0.6869933529419505
1107	2	0.5948040629463058
1107	3	0.6065540669979193
1107	4	0.7369726208838117
1107	5	0.6001345674685439
1107	6	0.5704449159412235
1107	7	0.6313382236263863
1107	8	0.5380991399384882
1107	9	0.7773944211826347
1107	10	0.7627245128181244
1107	11	0.7927492773076281
1107	12	0.6403972940041456
1107	13	0.7376266407288057
1107	14	0.8571791363657937
1107	15	0.8969379660759245
1107	16	0.9632050392302673
1107	17	0.8477913833626545
1107	18	0.9701415286975035
1107	19	1.2249855899576119
1107	20	1.0166512259944014
1107	21	1.078032994482328
1107	22	1.1144104546442435
1107	23	0.931226910590361
1107	24	1.1110743702496606
1107	25	1.1264423903143808
1108	1	0.7215681966633447
1108	2	0.46827985610928025
1108	3	0.5247582361711451
1108	4	0.6936330974354803
1108	5	0.6866115637806303
1108	6	0.7641806939340583
1108	7	0.7835718961082705
1108	8	0.5340535510493112
1108	9	0.536216642823625
1108	10	0.7067303454757763
1108	11	0.660198862744295
1108	12	0.8096182549541098
1108	13	0.6948873840657157
1108	14	0.8704000450049278
1108	15	0.7271003912060436
1108	16	0.5767947392276511
1108	17	0.6899486820313427
1108	18	0.8187075580438758
1108	19	0.7998920843472774
1108	20	0.6926438240118173
1108	21	0.8409308837402989
1108	22	0.7760764238331603
1108	23	0.766562876902185
1108	24	0.7794263175203398
1108	25	0.824652706010248
1109	1	0.0867971393342728
1109	2	0.0452548239369186
1109	3	0.0840617564501116
1109	4	0.19549598090926423
1109	5	0.30381754991537946
1109	6	0.07598795035814732
1109	7	0.33432120077015526
1109	8	0.42583247503951327
1109	9	0.5946380177250319
1109	10	0.3759855034316857
1109	11	0.3904543735583559
1109	12	0.4739100478886442
1109	13	0.403128178619248
1109	14	0.4038541111762086
1109	15	0.43065024798714546
1109	16	0.3774610184422258
1109	17	0.40855727931641184
1109	18	0.4542933025745768
1109	19	0.7319279720968765
1109	20	0.528302734717947
1109	21	0.590757049210012
1109	22	0.5847079568980433
1109	23	0.5684226469216966
1109	24	0.25175289811729584
1109	25	0.47011847876959934
1110	1	0.42429668554552435
1110	2	0.4740683349134187
1110	3	0.4456545679177424
1110	4	0.5536941284139445
1110	5	0.46098015149922844
1110	6	0.4221373583583201
1110	7	0.37772803923691356
1110	8	0.5331758790255918
1110	9	0.7188318944293286
1110	10	0.722530680046269
1110	11	0.735958104998403
1110	12	0.5761642110762608
1110	13	0.2885332304548882
1110	14	0.6646024217855313
1110	15	0.7328006232056126
1110	16	0.6967928080294641
1110	17	0.7380866744549053
1110	18	0.4988125717413183
1110	19	0.6207779287924637
1110	20	0.6277910554921391
1110	21	0.5877818157032948
1110	22	0.6185191081730608
1110	23	0.6367710114670473
1110	24	0.493065436689335
1110	25	0.6479829229950695
1111	1	-0.37733403563181156
1111	2	-0.13351423112503724
1111	3	-0.1834473856900587
1111	4	-0.03432361597864997
1111	5	0.2033223729975404
1111	6	-0.027019974617317644
1111	7	0.24964648401563494
1111	8	0.3625154465299758
1111	9	-0.06790005218419282
1111	10	-0.04148422830949983
1111	11	0.4591283342766488
1111	12	0.28061276479328967
1111	13	0.4026070632245293
1111	14	0.6449130444680762
1111	15	0.7219738609823949
1111	16	0.48283490784645977
1111	17	0.5625973143836234
1111	18	0.6451079986319286
1111	19	0.5863430761993058
1111	20	0.4835843314021133
1111	21	0.47434992370263984
1111	22	0.417208399259664
1111	23	0.5855594680306524
1111	24	0.6897867481647495
1111	25	0.7341467041220113
1112	1	-0.24403395150492102
1112	2	-0.5461480249830473
1112	3	-0.28165808416829563
1112	4	-0.49317093857913363
1112	5	-0.30813139394781075
1112	6	-0.3688038353598486
1112	7	-0.45772166809848613
1112	8	-0.33111904033239625
1112	9	-0.5504723493513453
1112	10	-0.33568278233305904
1112	11	-0.25545017411585036
1112	12	-0.18134414185233538
1112	13	-0.28717320739797714
1112	14	-0.27846066227127153
1112	15	-0.30753574742050205
1112	16	-0.43880728082943676
1112	17	-0.2083028565839271
1112	18	-0.30202377743830766
1112	19	-0.17196188940530277
1112	20	0.0037701518841970483
1112	21	-0.05791316908250366
1112	22	-0.18403808193952642
1112	23	0.011865039931343802
1112	24	-0.03702623266080707
1112	25	-0.09943693594462388
1113	1	-0.35973497075746863
1113	2	-0.8187582822229764
1113	3	-0.3610594755289185
1113	4	-0.302403101659543
1113	5	-0.31478533343980075
1113	6	-0.31390851097867767
1113	7	-0.10449662360730372
1113	8	0.05230888395170297
1113	9	-0.19283183810721027
1113	10	-0.3950492278920308
1113	11	-0.2068444468872528
1113	12	-0.22144250781253305
1113	13	-0.1787532106337011
1113	14	-0.281024001536764
1113	15	-0.13661777298271005
1113	16	-0.35862155036511467
1113	17	-0.13222843496929926
1113	18	-0.028666068329748384
1113	19	-0.2856205206947351
1113	20	-0.04047511587182839
1113	21	-0.348341902297193
1113	22	-0.3285292645960567
1113	23	-0.04381850642037932
1113	24	-0.2546861582391955
1113	25	-0.3093363049787641
1114	1	-0.9369622434847429
1114	2	-0.6509920484567431
1114	3	-0.6300854495548904
1114	4	-0.6078349198413591
1114	5	-0.6371749438294074
1114	6	-1.0616163031864687
1114	7	-0.7615972729579568
1114	8	-0.9992300770872617
1114	9	-0.9583123575877295
1114	10	-1.1396368902296956
1114	11	-0.9773834079262116
1114	12	-0.9785399104099355
1114	13	-0.8961785353090264
1114	14	-2.0952545210172815
1114	15	-1.285211928826861
1114	16	-1.256280641274202
1114	17	-1.4079199934108644
1114	18	-1.4505232111868906
1114	19	-1.2962309103051268
1114	20	-0.7870173236640383
1114	21	-0.8019295646348539
1114	22	-0.8916786152454037
1114	23	-1.0147925323944023
1114	24	-1.6043355088885463
1114	25	-1.5519207205631815
1115	1	-1.4958576283383884
1115	2	-1.334490577185253
1115	3	-0.9911186951281721
1115	4	-2.1684542089849224
1115	5	-1.3928922532094106
1115	6	-1.2639154875342058
1115	7	-1.0241711591796232
1115	8	-0.912926453113279
1115	9	-1.5065571565139635
1115	10	-1.2987526431593324
1115	11	-1.1848475295513197
1115	12	-1.7197051832404568
1115	13	-1.191606946335817
1115	14	-2.0583949758208604
1115	15	-2.0648280431214303
1115	16	-1.5674051960209923
1115	17	-1.6479801993888685
1115	18	-1.396931501930787
1115	19	-1.2228389842300658
1115	20	-1.368076316488363
1115	21	-1.3954433868433114
1115	22	-1.5101062906364575
1115	23	-2.117473085798977
1115	24	-1.6873500667327477
1115	25	-1.4418425108682698
1116	1	-0.91710108513481
1116	2	-0.9605275423588857
1116	3	-0.8837089372377065
1116	4	-1.0321070033164048
1116	5	-1.0154850356616834
1116	6	-1.494873312078166
1116	7	-0.8468886217748235
1116	8	-1.2189758377402882
1116	9	-1.095115719912208
1116	10	-1.1479294294649094
1116	11	-0.9577251065803125
1116	12	-1.6382497155700406
1116	13	-1.2439534855058128
1116	14	-1.7035002938174415
1116	15	-1.6247851395552075
1116	16	-1.918935820808727
1116	17	-1.69376099967878
1116	18	-2.4576717948320024
1116	19	-1.7665712727753657
1116	20	-1.6754098337461514
1116	21	-1.606173466777605
1116	22	-2.89471235996141
1116	23	-2.587251353637299
1116	24	-2.025467863973333
1116	25	-2.3557166974350494
1201	1	0.9306393478373209
1201	2	0.8044041301677822
1201	3	0.7054508645247282
1201	4	0.8726689758692316
1201	5	0.8123597734218464
1201	6	0.8645654874638282
1201	7	0.8990901203573927
1201	8	0.9329962595682133
1201	9	1.1713308338664845
1201	10	0.8283277730597689
1201	11	0.9750882346044847
1201	12	0.8251747804110536
1201	13	0.8319789863032199
1201	14	0.961588095028084
1201	15	0.8398840873616749
1201	16	0.986025330313268
1201	17	0.8357604894920598
1201	18	0.8954034998972986
1201	19	0.9505850689911668
1201	20	1.0728812915003303
1201	21	0.8809200168859874
1201	22	1.0147864747826745
1201	23	1.0884355387470563
1201	24	1.0592389088197294
1201	25	0.9148253286631842
1202	1	0.8458755429820748
1202	2	1.0462111664884084
1202	3	0.8848732775006098
1202	4	0.9414774240274397
1202	5	0.9789323658262887
1202	6	1.040774540736635
1202	7	0.7956098792088753
1202	8	0.9055855523251424
1202	9	1.1435286329629335
1202	10	0.9974266212289749
1202	11	0.9632446468147435
1202	12	0.9668100950207119
1202	13	1.0037325377875277
1202	14	0.9225288231773519
1202	15	1.201491802030091
1202	16	1.0600690111641597
1202	17	1.1196534561383942
1202	18	1.0529116753125152
1202	19	1.2253260572195828
1202	20	1.3253245086374719
1202	21	1.3015915334426964
1202	22	1.1521670999712725
1202	23	1.137871201720948
1202	24	1.2345072194703093
1202	25	1.3328435892011328
1203	1	0.45673063770813727
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2211	8	0.28740894178860543
2211	9	0.12684196177711726
2211	10	-0.010493993652588074
2211	11	0.1780092257558934
2211	12	0.17643603469758773
2211	13	0.17422770318931668
2211	14	-0.869227587424561
2211	15	-0.4072343143360939
2211	16	0.07200193141626698
2211	17	0.14102546902144297
2211	18	-0.08709059117532547
2211	19	0.09790521672573504
2211	20	0.047911234619782306
2211	21	0.020229526274235354
2211	22	0.13757831203783866
2211	23	0.09419190422160995
2211	24	-0.08389147923399065
2211	25	-0.18597482435412616
2212	1	-0.1711302930620917
2212	2	-0.09338431107148182
2212	3	-0.014879050135714778
2212	4	0.04062713222522518
2212	5	-0.06345716132668855
2212	6	-0.14333789733675673
2212	7	-0.16219721850584534
2212	8	-0.3408528795557544
2212	9	-0.46760292440824713
2212	10	-0.3265051809520969
2212	11	-0.29717221153561013
2212	12	-0.2407507956456385
2212	13	-0.3395635765370528
2212	14	-0.22780877865354654
2212	15	-0.08821943543890143
2212	16	-0.10669000444383414
2212	17	-0.3963842494913479
2212	18	-0.10347529333178329
2212	19	-0.16942102595103137
2212	20	-0.3507856040146038
2212	21	-0.26613670304692505
2212	22	-0.19837687277794913
2212	23	-0.5791147332967483
2212	24	-0.27557312751635266
2212	25	-0.4069886560503946
2213	1	-0.35701020011526907
2213	2	-0.42148829532913723
2213	3	-0.3993098031829163
2213	4	-0.502325120008166
2213	5	-0.44790263357086246
2213	6	-0.24855333404551772
2213	7	-0.9357476065693007
2213	8	-0.5368968827586542
2213	9	-0.6719904226419402
2213	10	-0.5810099094123515
2213	11	-0.49658691084530915
2213	12	-0.8362963741297023
2213	13	-0.6817342067018544
2213	14	-0.6202284576311285
2213	15	-0.6542121373339498
2213	16	-0.8937545778880462
2213	17	-0.777795931692765
2213	18	-0.9533901836639558
2213	19	-1.2674986334077118
2213	20	-0.596697265282053
2213	21	-1.42587139115696
2213	22	-0.8470580981478264
2213	23	-1.0628846925278452
2213	24	-1.1335682028001415
2213	25	-1.0832072093037937
2214	1	-0.8143928180214495
2214	2	-0.8445168940260857
2214	3	-0.6393362558742481
2214	4	-1.077284641046191
2214	5	-0.9123621739174226
2214	6	-2.3958677928273175
2214	7	-0.9329240782933326
2214	8	-1.580785180132139
2214	9	-0.9724243129805075
2214	10	-1.327444726560536
2214	11	-0.9633131351442756
2214	12	-0.9414813360918899
2214	13	-1.1985267696062962
2214	14	-1.2715846062958036
2214	15	-1.5590027914519098
2214	16	-1.0317688744781606
2214	17	-1.1960353244117883
2214	18	-1.4116627181021073
2214	19	-3.1973665906200033
2214	20	-1.8370750643298663
2214	21	-2.2924583871352624
2214	22	-1.9416123330609665
2214	23	-1.8015729948663832
2214	24	-1.2000674828676594
2214	25	-0.9579517160259243
2215	1	-1.7159418272362608
2215	2	-1.512702998749912
2215	3	-1.4921410726070299
2215	4	-1.3738011260639595
2215	5	-2.248500283219826
2215	6	-1.9229145705785768
2215	7	-2.2872730824767338
2215	8	-3.015061913757272
2215	9	-2.639948298636803
2215	10	-1.9310890718365812
2215	11	-2.462898568245265
2215	12	-3.06192760783744
2215	13	-2.8812291797546443
2215	14	-2.83463668519245
2215	15	-3.688317615267092
2215	16	-3.301182851933966
2215	17	-2.9895034411949197
2215	18	-3.3509444778922948
2215	19	-4.224461061908318
2215	20	-3.671648021989533
2215	21	-2.8885444423486604
2215	22	-4.037527355407047
2215	23	-3.9311215694236132
2215	24	-2.9661270148840018
2215	25	-3.1428918789805884
2216	1	-1.2280447657460805
2216	2	-1.1233362190924865
2216	3	-1.412478284510371
2216	4	-0.9392687053041868
2216	5	-0.9661768075693118
2216	6	-1.4488626274772116
2216	7	-2.8763065813033677
2216	8	-2.052147347743709
2216	9	-2.120136751872259
2216	10	-2.170496022555227
2216	11	-2.5284214075120452
2216	12	-2.0242300118802206
2216	13	-2.4798928136275187
2216	14	-2.6668027194020425
2216	15	-2.609462550073495
2216	16	-2.5031135776999633
2216	17	-2.448361855079945
2216	18	-2.383609771551356
2216	19	-2.550201711713285
2216	20	-2.740331933314067
2216	21	-2.812913981698429
2216	22	-2.975844591638033
2216	23	-2.3022174650926814
2216	24	-2.324293057035156
2216	25	-2.261141056036358
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36107.0
3	1.0
4	554.0
5	4968.0
6	5663.0
7	4634.0
8	3609.0
9	3307.0
10	2601.0
11	2317.0
12	2178.0
13	2055.0
14	1916.0
15	1892.0
16	1950.0
17	2044.0
18	2287.0
19	2725.0
20	3356.0
21	4525.0
22	5786.0
23	6945.0
24	7984.0
25	9273.0
26	10248.0
27	11545.0
28	12432.0
29	15134.0
30	18996.0
31	24275.0
32	31045.0
33	39748.0
34	51322.0
35	67861.0
36	93029.0
37	144752.0
38	465503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.7168254808141	19.38994347200918	13.613560194291752	27.279670852884973
2	14.272030824776428	20.847373538566277	38.169199486495145	26.711396150162148
3	16.54811342363116	24.54400683706828	30.651286884362833	28.25659285493773
4	11.749645901330542	17.302655649458618	37.04379231524381	33.90390613396703
5	13.202511105453564	37.58558424697188	35.61201626836828	13.599888379206279
6	31.989653079160284	37.342599052287284	17.411548323382046	13.25619954517038
7	27.1845706574407	32.17927236828997	22.321556338474636	18.314600635794697
8	25.479270351152344	35.627325680953945	20.53167999898524	18.36172396890847
9	25.960033335748967	14.998641205884484	19.91856293934343	39.12276251902312
10	14.606068406436034	28.90142918258877	34.367545010912835	22.12495740006236
11	33.997401550921005	22.906007668593624	23.998352203023074	19.0982385774623
12	22.144229602545508	25.35490565730212	31.386173936822352	21.11469080333002
13	28.32707245148609	21.861297247475502	25.917425170465297	23.894205130573113
14	21.450941867255796	21.372532153867745	27.71348764775387	29.463038331122586
15	23.299029931423377	29.397767954960713	23.88619576298963	23.41700635062628
16	22.898420011735816	28.163327030766666	26.193141068828375	22.745111888669143
17	21.904962872867532	27.99700048633419	27.435678454308587	22.66235818648969
18	22.024112921697736	27.589622227272027	28.333271468186677	22.052993382843557
19	23.00248707346509	27.182617466009845	27.965135702586934	21.849759757938127
20	23.15443229598618	26.857066286157156	28.010183941098287	21.978317476758377
21	23.12441832252364	26.568944850637898	27.763805447217184	22.54283137962128
22	22.964585185493668	26.78327958673361	27.662267352064067	22.58986787570865
23	22.687532761706624	26.674796549236035	27.695920663934377	22.941750025122968
24	22.643443086748018	26.72748687947404	27.95556992015876	22.673500113619184
25	22.814822862626865	26.64855908955102	27.805271115698364	22.731346932123746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	295.0
1	295.0
2	428.5
3	562.0
4	562.0
5	562.0
6	1223.0
7	1884.0
8	1884.0
9	1884.0
10	2551.5
11	3219.0
12	3219.0
13	3219.0
14	4795.5
15	6372.0
16	6372.0
17	6372.0
18	10693.0
19	15014.0
20	15014.0
21	15014.0
22	22319.0
23	29624.0
24	29624.0
25	29624.0
26	40447.5
27	51271.0
28	51271.0
29	51271.0
30	63127.5
31	74984.0
32	74984.0
33	74984.0
34	84976.5
35	94969.0
36	94969.0
37	94969.0
38	104556.0
39	114143.0
40	114143.0
41	114143.0
42	121653.5
43	129164.0
44	129164.0
45	129164.0
46	134199.5
47	139235.0
48	139235.0
49	139235.0
50	137525.0
51	135815.0
52	135815.0
53	135815.0
54	125166.5
55	114518.0
56	114518.0
57	114518.0
58	99723.5
59	84929.0
60	84929.0
61	84929.0
62	69965.5
63	55002.0
64	55002.0
65	55002.0
66	42974.0
67	30946.0
68	30946.0
69	30946.0
70	22472.0
71	13998.0
72	13998.0
73	13998.0
74	9822.0
75	5646.0
76	5646.0
77	5646.0
78	3882.5
79	2119.0
80	2119.0
81	2119.0
82	1366.5
83	614.0
84	614.0
85	614.0
86	405.0
87	196.0
88	196.0
89	196.0
90	119.5
91	43.0
92	43.0
93	43.0
94	24.0
95	5.0
96	5.0
97	5.0
98	2.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07840176286273264
2	9.05332134673587E-5
3	0.0
4	0.032139290780912336
5	0.07514256717790772
6	0.07758696394152641
7	0.03956301428523575
8	0.07767749715499377
9	0.05857498911338108
10	0.11579198002475179
11	0.005613059234976239
12	0.017110777345330796
13	0.008147989212062282
14	0.010139719908344174
15	0.009868120267942098
16	0.02308596943417647
17	0.03512688682533518
18	0.0012674649885430218
19	0.005069859954172087
20	0.0075142567177907725
21	2.715996404020761E-4
22	9.05332134673587E-5
23	0.0
24	0.0
25	0.005160393167639446
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1104567.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.28126052668533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.35856213130293	62.75337632936503
2	11.59962704382496	17.46469090997143
3	2.8071448484036146	6.339762080064452
4	0.9812338169361173	2.954740744414469
5	0.45589148296992205	1.716004275067782
6	0.23248302514833882	1.0500969110534422
7	0.139842079324795	0.7369241604370302
8	0.0934039789990072	0.5625255421802646
9	0.0600339574260456	0.40674887904942664
>10	0.2527417098364476	3.1011127558173692
>50	0.01193517997330323	0.6139955259603965
>100	0.0060175850133192925	0.8793840340076171
>500	4.8140481831281215E-4	0.22876980726044072
>1k	6.017560228910151E-4	1.1918680453509296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4466	0.4043213313452239	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3226	0.2920601466456992	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2849	0.25792912516850497	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1466	0.13272169094314787	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1148	0.10393212906052779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	9.053321346735871E-5	0.0	0.0	0.0
8	0.0	9.053321346735871E-5	0.0	0.0	0.0
9	0.0	9.053321346735871E-5	0.0	0.0	0.0
10	0.0	9.053321346735871E-5	0.0	0.0	0.0
11	0.0	9.053321346735871E-5	0.0	9.053321346735871E-5	0.0
12	0.0	9.053321346735871E-5	0.0	9.053321346735871E-5	0.0
13	0.0	9.053321346735871E-5	0.0	9.053321346735871E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAC	150	1.4551915E-11	12.0402565	1
ACTACAC	105	2.6887756E-7	11.771341	3
GGTATCA	710	0.0	11.112023	1
AATCCCG	60	0.0059341798	11.068123	19
GACTGCG	95	1.348419E-5	11.007826	7
TCTACAC	200	0.0	10.933765	3
TCCAACG	70	0.001507986	10.842243	18
CTAGGAC	160	5.347829E-10	10.6960745	3
TTGGACC	90	9.349284E-5	10.573138	4
GTCTAGA	100	2.3814742E-5	10.456013	1
ATCTCCG	75	0.0026309588	10.140543	7
CTGCGCA	85	6.560426E-4	10.063698	9
TAGGACT	95	1.6154755E-4	10.016657	4
CCCTTAA	115	1.0182874E-5	9.918747	1
GTATTAC	125	2.5476747E-6	9.885685	1
CTTGGAC	135	6.3623156E-7	9.8597555	3
CCAGTAC	175	2.5302143E-9	9.779268	3
CTACACT	205	4.0017767E-11	9.747917	4
TACACAG	295	0.0	9.67711	5
TGGGTTG	90	0.0010961564	9.515824	5
>>END_MODULE