FastQCFastQC Report
Thu 26 May 2016
SRR1512948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390816
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT86340.36113193152463424No Hit
CTGTAGGACGTGGAATATGGCAAGA77060.3223167320278934No Hit
GTCCTAAAGTGTGTATTTCTCATTT75740.31679560451327077No Hit
CTTTAGGACGTGAAATATGGCGAGG65110.27233379733112045No Hit
GTCCTACAGTGTGCATTTCTCATTT53610.22423306519615058No Hit
GTATCAACGCAGAGTACTTTTTTTT47040.19675290779382434No Hit
CTGTAGGACCTGGAATATGGCGAGA39010.16316604874653673No Hit
CTGAAGGACCTGGAATATGGCGAGA35010.14643535930828636No Hit
GTCCTTCAGTGTGCATTTCTCATTT29920.12514555699811278No Hit
GGTATCAACGCAGAGTACTTTTTTT27580.11535810367673631No Hit
ATTTAGAAATGTCCACTGTAGGACG27390.11456339592841942No Hit
TATCAACGCAGAGTACTTTTTTTTT26950.11272302009021189No Hit
TTGTAGAACAGTGTATATCAATGAG26550.11104995114638684No Hit
TTTCTAAATTTTCCACCTTTTTCAG26130.10929322875537055No Hit
GATATACACTGTTCTACAAATCCCG24670.10318652711040917No Hit
GTGTATATCAATGAGTTACAATGAA24360.10188989867894475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG400.005286688514.2473634
GCGCCAC707.247374E-613.57488313
TAGGACC14300.013.5499394
TCCAACG3550.013.38284718
TGGGCGA655.4815515E-513.1470110
GGTATCA12300.013.0148811
CCAACGA2550.012.65928919
TATGTCG1551.8189894E-1212.26041516
AATGTCC8750.011.93648
CTCGAAC1459.640644E-1111.79536418
TCGAACT1459.822543E-1111.78623419
GGCGAGG17150.011.57051719
GCCTCGA1657.2759576E-1211.51735916
GGACCTG25050.011.4843256
ATGTCGA1751.8189894E-1211.40218517
ATCCCGT2500.011.39407510
AGGACCT26300.011.3401075
AGGACGA1104.983758E-711.2251955
TGTCGAG1701.0913936E-1111.17861418
GTCCTAC43250.011.1701211
Produced by FastQC (version 0.11.5)