FastQCFastQC Report
Thu 26 May 2016
SRR1512948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390816
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT86000.359709822922383No Hit
CTGTAGGACGTGGAATATGGCAAGA76650.3206018363604728No Hit
CTTTAGGACGTGAAATATGGCGAGG69300.28985919451768766No Hit
GTCCTAAAGTGTGTATTTCTCATTT69020.28868804625701017No Hit
GTATCAACGCAGAGTACTTTTTTTT57490.24046183395125348No Hit
GTCCTACAGTGTGCATTTCTCATTT52130.21804271010399798No Hit
TATCAACGCAGAGTACTTTTTTTTT41700.17441743739376012No Hit
CTGTAGGACCTGGAATATGGCGAGA40110.1677669883420556No Hit
CTGAAGGACCTGGAATATGGCGAGA37250.15580454539370658No Hit
GGTATCAACGCAGAGTACTTTTTTT34890.14593343862513886No Hit
ATTTAGAAATGTCCACTGTAGGACG28520.11928981569472513No Hit
TTTCTAAATTTTCCACCTTTTTCAG28380.11870424156438639No Hit
TTGTAGAACAGTGTATATCAATGAG27840.11644559849022257No Hit
GTCCTTCAGTGTGCATTTCTCATTT26650.1114682183823431No Hit
GATATACACTGTTCTACAAATCCCG26380.1103388968452612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAG508.796446E-515.18385318
CCAACGA2050.014.81351519
TCCAACG3150.014.76207918
TAGGACC15900.013.9439254
CTGTCCG551.9579876E-413.8205599
CCAACGT1400.013.55701319
GGTATCA11550.013.4179431
GGCGAGG17300.012.39722119
ATAGCGA550.00308896112.08059117
GTCCTAT3500.011.6810191
AGGACCT28800.011.6629525
GGACCTG28150.011.4218666
TGTAGGA44200.011.3787752
TATACTG5050.011.1169825
GTCCTAA21850.011.0960661
TGACGGT600.00587428211.08407910
GTCGAGA600.005923901711.07155919
GTATCAA26150.011.0166921
ACTGTTC10900.010.98638
CTGTAGG45000.010.9445411
Produced by FastQC (version 0.11.5)