##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512948_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2390816 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.612506357661985 31.0 31.0 34.0 27.0 34.0 2 30.739882533829455 31.0 31.0 34.0 27.0 34.0 3 30.84017339686534 31.0 31.0 34.0 27.0 34.0 4 34.31186381553411 37.0 35.0 37.0 31.0 37.0 5 34.00999407733594 37.0 35.0 37.0 30.0 37.0 6 33.905866030677394 37.0 35.0 37.0 30.0 37.0 7 33.78212668812657 37.0 35.0 37.0 29.0 37.0 8 33.67190239650395 37.0 35.0 37.0 28.0 37.0 9 35.12948717090734 39.0 35.0 39.0 28.0 39.0 10 35.005115408295744 39.0 35.0 39.0 28.0 39.0 11 35.03891851150402 39.0 35.0 39.0 28.0 39.0 12 34.90372073802417 39.0 35.0 39.0 27.0 39.0 13 34.899393763468204 39.0 35.0 39.0 27.0 39.0 14 35.95914281985732 40.0 36.0 41.0 27.0 41.0 15 36.003082629529 40.0 36.0 41.0 27.0 41.0 16 35.99372473665895 40.0 36.0 41.0 27.0 41.0 17 35.9932437293376 40.0 36.0 41.0 27.0 41.0 18 35.91776782487653 40.0 36.0 41.0 27.0 41.0 19 35.922120732001126 40.0 36.0 41.0 27.0 41.0 20 35.89606477453723 40.0 36.0 41.0 27.0 41.0 21 35.84908248899121 40.0 36.0 41.0 27.0 41.0 22 35.81496066614913 40.0 36.0 41.0 27.0 41.0 23 35.712614856182995 40.0 36.0 41.0 27.0 41.0 24 35.70872831702649 40.0 36.0 41.0 27.0 41.0 25 35.625077797705885 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5872914147642199 1101 2 0.5707805872223126 1101 3 0.5270149447155603 1101 4 0.3588057187758835 1101 5 0.620740575874855 1101 6 0.7037073628316932 1101 7 0.7030801085663612 1101 8 0.717308738368672 1101 9 0.5716767527318041 1101 10 0.9375770428614345 1101 11 0.897021590965096 1101 12 0.9748208591377718 1101 13 1.0584346911985065 1101 14 1.1610102560761533 1101 15 1.0868526090427366 1101 16 1.255824084120043 1101 17 1.099155525197915 1101 18 0.5589272463564754 1101 19 0.9485090581808393 1101 20 0.8630203374207852 1101 21 1.0430150279257688 1101 22 1.0255020976069957 1101 23 0.9752703293732665 1101 24 0.9373732926195331 1101 25 1.0135505162540497 1102 1 0.6385546820012671 1102 2 0.5783771422399475 1102 3 0.5242646790534451 1102 4 0.4192931182144619 1102 5 0.5808987260450778 1102 6 0.418885238834136 1102 7 0.6051718756917666 1102 8 0.6696437646212416 1102 9 0.6719685143311409 1102 10 0.8485843036703216 1102 11 0.7224965530106928 1102 12 0.6979718844105847 1102 13 0.8769168587922849 1102 14 0.9567627986287661 1102 15 0.917342330924555 1102 16 0.9130508777967208 1102 17 0.9131228183268476 1102 18 0.9460093092536184 1102 19 0.9718160829368827 1102 20 0.916814993112979 1102 21 0.8826163443254273 1102 22 0.9598077953593034 1102 23 0.9267278305288897 1102 24 0.9888664892050159 1102 25 1.0364462221237076 1103 1 0.4481729574639459 1103 2 0.4330346210672573 1103 3 0.4689374161834863 1103 4 0.5115457817432159 1103 5 0.6111724924431385 1103 6 0.5570770991559755 1103 7 0.5289655867245671 1103 8 0.5837737127881937 1103 9 0.5676478271119691 1103 10 0.7647267870566381 1103 11 0.714032610248843 1103 12 0.8196850858201827 1103 13 0.8237977372433676 1103 14 0.9902211966507295 1103 15 0.7676328136865749 1103 16 0.8802583619634063 1103 17 0.9698855527461276 1103 18 1.1010956839794588 1103 19 1.0478498688852795 1103 20 0.999762761663213 1103 21 0.6967684714519393 1103 22 0.9259448366860923 1103 23 0.9296239303177813 1103 24 0.980274709384581 1103 25 1.059580622511426 1104 1 0.5263528378020972 1104 2 0.6375335760158514 1104 3 0.5744214272430384 1104 4 0.5178738361827797 1104 5 0.48855651596025496 1104 6 0.6708153293510435 1104 7 0.6407951706764479 1104 8 0.6012396102001318 1104 9 0.7580545926086657 1104 10 0.8410477649063921 1104 11 0.8231362364387991 1104 12 0.7805925503247693 1104 13 0.8154418678263511 1104 14 0.9674355290808947 1104 15 0.9546881477742843 1104 16 0.9584289423821915 1104 17 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2216 14 -2.1821241712903117 2216 15 -2.140401463780833 2216 16 -1.9388277394878202 2216 17 -2.0949596540279956 2216 18 -1.8422097461902567 2216 19 -2.116399446003257 2216 20 -2.5300097101751504 2216 21 -2.3343311947804892 2216 22 -2.455819612688636 2216 23 -1.7046902253682532 2216 24 -1.8553843690568783 2216 25 -1.6931173269587916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54578.0 3 2.0 4 879.0 5 7631.0 6 9297.0 7 7380.0 8 5920.0 9 5308.0 10 4458.0 11 4067.0 12 3580.0 13 3425.0 14 3458.0 15 3452.0 16 3409.0 17 3532.0 18 4015.0 19 4823.0 20 6320.0 21 8301.0 22 10648.0 23 13143.0 24 15693.0 25 18136.0 26 21058.0 27 22975.0 28 26107.0 29 31232.0 30 39233.0 31 51223.0 32 65004.0 33 84440.0 34 110932.0 35 146252.0 36 202395.0 37 319376.0 38 1069134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.5957803420299 20.5753744169945 13.958769549678221 26.870075691297384 2 12.049368959693226 21.38443224776164 43.621335662247255 22.944863130297882 3 16.970021950664542 25.923073963031868 30.661247038667973 26.445657047635617 4 10.372984267432724 18.325164827532536 40.15432158167488 31.147529323359862 5 11.084814637768638 39.623766951667605 37.458675143302756 11.832743267261002 6 29.432726386831387 39.91246374457765 18.96583984699573 11.688970021595233 7 26.12049995543734 32.26290884152554 23.639133634853106 17.977457568184015 8 22.584763445932683 40.74438548516114 21.16720126277139 15.503649806134788 9 25.5407762537204 15.246525673689531 21.271857873186672 37.9408401994034 10 13.324517044360059 30.709203167163874 36.431178507238684 19.535101281237385 11 32.616848621742584 22.673108294641736 27.02685405948049 17.68318902413519 12 22.640620672864138 25.455502297068573 34.07450852103255 17.829368509034747 13 28.528630778986752 23.257038689156563 26.597519329519574 21.61681120233711 14 18.673259681311595 24.42015869583132 29.098920736163265 27.807660886693817 15 20.83859001559436 34.65716671240119 23.906763467703612 20.597479804300832 16 19.482460727508524 29.67000648440605 31.09912774279917 19.748405045286255 17 19.400949269638097 30.6686309228936 29.880989837860998 20.049429969607306 18 19.039120156199544 28.965470798749198 33.30027589229994 18.69513315275131 19 22.212899096921 27.677886485604695 28.982561665781294 21.126652751693012 20 22.08132168017622 29.154950904647848 30.073737144731105 18.689990270444824 21 20.888358154018608 28.068545663298917 28.561181019861426 22.481915162821046 22 21.225469057100803 30.728954700233018 28.879704635296783 19.165871607369393 23 19.912741089234807 29.968094575241256 29.735245204984405 20.38391913053953 24 21.645454940907204 28.162769531406852 30.898781001967528 19.292994525718417 25 20.888785526386787 29.175609219470427 30.158868258681583 19.776736995461206 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 125.0 1 125.0 2 354.0 3 583.0 4 583.0 5 583.0 6 1579.5 7 2576.0 8 2576.0 9 2576.0 10 3904.0 11 5232.0 12 5232.0 13 5232.0 14 9238.0 15 13244.0 16 13244.0 17 13244.0 18 24413.0 19 35582.0 20 35582.0 21 35582.0 22 58570.5 23 81559.0 24 81559.0 25 81559.0 26 118540.5 27 155522.0 28 155522.0 29 155522.0 30 202651.0 31 249780.0 32 249780.0 33 249780.0 34 273733.5 35 297687.0 36 297687.0 37 297687.0 38 307739.5 39 317792.0 40 317792.0 41 317792.0 42 320663.5 43 323535.0 44 323535.0 45 323535.0 46 316463.5 47 309392.0 48 309392.0 49 309392.0 50 280500.5 51 251609.0 52 251609.0 53 251609.0 54 206489.5 55 161370.0 56 161370.0 57 161370.0 58 129581.0 59 97792.0 60 97792.0 61 97792.0 62 74674.0 63 51556.0 64 51556.0 65 51556.0 66 37335.0 67 23114.0 68 23114.0 69 23114.0 70 15971.5 71 8829.0 72 8829.0 73 8829.0 74 5809.5 75 2790.0 76 2790.0 77 2790.0 78 1825.5 79 861.0 80 861.0 81 861.0 82 543.0 83 225.0 84 225.0 85 225.0 86 136.5 87 48.0 88 48.0 89 48.0 90 29.5 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07955442827888053 2 8.365344719125186E-5 3 0.0 4 0.031035428907954438 5 0.07604098349684794 6 0.0779231860586511 7 0.03860606587876273 8 0.07466070161819229 9 0.05387281999116619 10 0.1176167467509001 11 0.004851899937092608 12 0.0158105015191466 13 0.007194196458447659 14 0.008574478337103316 15 0.008699958507890194 16 0.019491253195561685 17 0.03212292372144072 18 0.0011711482606775261 19 0.0044336327011363485 20 0.0067759292224914 21 4.182672359562593E-5 22 3.764405123606334E-4 23 0.0 24 0.0 25 0.0038898852943932112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2390816.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.41736107672972 #Duplication Level Percentage of deduplicated Percentage of total 1 89.01927265474178 65.3556008328103 2 8.191300899467578 12.027673916487043 3 1.402732933717976 3.0895485086697905 4 0.46172486103036053 1.3559448336147533 5 0.22724970069294392 0.834203666517631 6 0.12991628823960227 0.5722866626061226 7 0.08250227025146886 0.42399692752914186 8 0.06564642113364096 0.38556696030108634 9 0.045532850143420074 0.30086115298488864 >10 0.31748331382356515 4.396456139241823 >50 0.028608580037889658 1.4405569114839762 >100 0.022508843503376622 3.391587358934254 >500 0.0029024695262085194 1.428800426177102 >1k 0.002276446687218197 3.2813607635171516 >5k 3.414670030827296E-4 1.7155549391251013 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 8600 0.359709822922383 No Hit CTGTAGGACGTGGAATATGGCAAGA 7665 0.3206018363604728 No Hit CTTTAGGACGTGAAATATGGCGAGG 6930 0.28985919451768766 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6902 0.28868804625701017 No Hit GTATCAACGCAGAGTACTTTTTTTT 5749 0.24046183395125348 No Hit GTCCTACAGTGTGCATTTCTCATTT 5213 0.21804271010399798 No Hit TATCAACGCAGAGTACTTTTTTTTT 4170 0.17441743739376012 No Hit CTGTAGGACCTGGAATATGGCGAGA 4011 0.1677669883420556 No Hit CTGAAGGACCTGGAATATGGCGAGA 3725 0.15580454539370658 No Hit GGTATCAACGCAGAGTACTTTTTTT 3489 0.14593343862513886 No Hit ATTTAGAAATGTCCACTGTAGGACG 2852 0.11928981569472513 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2838 0.11870424156438639 No Hit TTGTAGAACAGTGTATATCAATGAG 2784 0.11644559849022257 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2665 0.1114682183823431 No Hit GATATACACTGTTCTACAAATCCCG 2638 0.1103388968452612 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.2548017078687777E-4 0.0 5 0.0 0.0 0.0 1.2548017078687777E-4 0.0 6 0.0 0.0 0.0 1.2548017078687777E-4 0.0 7 0.0 0.0 0.0 1.2548017078687777E-4 0.0 8 0.0 0.0 0.0 1.2548017078687777E-4 0.0 9 0.0 0.0 0.0 1.6730689438250373E-4 0.0 10 0.0 0.0 0.0 2.0913361797812965E-4 0.0 11 0.0 0.0 0.0 2.0913361797812965E-4 0.0 12 0.0 0.0 0.0 2.0913361797812965E-4 0.0 13 0.0 0.0 0.0 2.0913361797812965E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGAG 50 8.796446E-5 15.183853 18 CCAACGA 205 0.0 14.813515 19 TCCAACG 315 0.0 14.762079 18 TAGGACC 1590 0.0 13.943925 4 CTGTCCG 55 1.9579876E-4 13.820559 9 CCAACGT 140 0.0 13.557013 19 GGTATCA 1155 0.0 13.417943 1 GGCGAGG 1730 0.0 12.397221 19 ATAGCGA 55 0.003088961 12.080591 17 GTCCTAT 350 0.0 11.681019 1 AGGACCT 2880 0.0 11.662952 5 GGACCTG 2815 0.0 11.421866 6 TGTAGGA 4420 0.0 11.378775 2 TATACTG 505 0.0 11.116982 5 GTCCTAA 2185 0.0 11.096066 1 TGACGGT 60 0.005874282 11.084079 10 GTCGAGA 60 0.0059239017 11.071559 19 GTATCAA 2615 0.0 11.016692 1 ACTGTTC 1090 0.0 10.9863 8 CTGTAGG 4500 0.0 10.944541 1 >>END_MODULE