FastQCFastQC Report
Thu 26 May 2016
SRR1512947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006541
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46350.2309945323818452No Hit
CTGTAGGACGTGGAATATGGCAAGA43130.21494701578487554No Hit
GTCCTAAAGTGTGTATTTCTCATTT43030.2144486457042243No Hit
CTTTAGGACGTGAAATATGGCGAGG37520.18698845426034155No Hit
GTATCAACGCAGAGTACTTTTTTTT28710.14308205015496817No Hit
GTCCTACAGTGTGCATTTCTCATTT28560.14233449503399134No Hit
CTGTAGGACCTGGAATATGGCGAGA20790.10361113976739074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT551.9704885E-413.8099876
TAGGACC7550.013.460964
GCACCGT853.971174E-612.2868266
CTACACG550.003073448512.0885614
TATTCCG550.003074022512.0882595
GTCCTAT2700.011.9980371
TCGTTGG658.008076E-411.69528612
GGCGAGG9800.011.43256319
CCAACGA1501.7826096E-1011.39380719
AGGACCT14750.011.2043245
CGTAAAC600.00588516711.0811823
ATTATAC2000.010.9228783
GGACCTG14450.010.7099086
ACGACAG803.779349E-410.6851585
TGTAGGA23400.010.6349522
TCCAACG2001.8189894E-1210.45240118
GTCCTAA13200.010.3940221
GTAGGAC22650.010.2739423
AATCCCG2500.010.25442819
GTTAGGT750.00265241510.1313653
Produced by FastQC (version 0.11.5)