##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512947_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2006541 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.101828968358983 31.0 30.0 34.0 23.0 34.0 2 29.05259000439064 31.0 30.0 34.0 22.0 34.0 3 29.314176984173262 31.0 30.0 34.0 23.0 34.0 4 32.73725929348067 35.0 33.0 37.0 25.0 37.0 5 32.91024753543536 37.0 35.0 37.0 26.0 37.0 6 32.90378716407988 37.0 35.0 37.0 26.0 37.0 7 32.766767785956034 37.0 35.0 37.0 25.0 37.0 8 32.76452412385294 37.0 35.0 37.0 25.0 37.0 9 34.10242701245576 39.0 35.0 39.0 25.0 39.0 10 33.97893589017119 39.0 34.0 39.0 24.0 39.0 11 34.05294683736839 39.0 35.0 39.0 25.0 39.0 12 33.95092699326852 39.0 35.0 39.0 23.0 39.0 13 33.947261979695405 39.0 35.0 39.0 23.0 39.0 14 34.938397471070864 39.0 35.0 41.0 23.0 41.0 15 34.89846158139804 39.0 35.0 41.0 22.0 41.0 16 34.78527226705061 39.0 34.0 41.0 21.0 41.0 17 34.727238566269016 39.0 34.0 41.0 19.0 41.0 18 34.42238060423385 39.0 34.0 41.0 17.0 41.0 19 34.665629558528835 39.0 34.0 41.0 18.0 41.0 20 34.75797155403254 39.0 34.0 41.0 19.0 41.0 21 34.68825655693056 39.0 34.0 41.0 18.0 41.0 22 34.59674235413082 39.0 34.0 41.0 18.0 41.0 23 34.32402228511653 39.0 34.0 41.0 16.0 41.0 24 34.341171199591734 39.0 34.0 41.0 16.0 41.0 25 34.25655742892869 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6423909285034775 1101 2 0.808655182029657 1101 3 0.8407956482333709 1101 4 0.861527801344721 1101 5 0.6215823349605287 1101 6 0.8650665729793019 1101 7 0.9654553777341519 1101 8 0.7419127992128196 1101 9 0.680218770380705 1101 10 0.7484554468251687 1101 11 0.8854760093768519 1101 12 0.9798865707539406 1101 13 0.9359733745129688 1101 14 0.9224945395295521 1101 15 0.6907345482850218 1101 16 1.1137202523843115 1101 17 1.1399266113563868 1101 18 1.4002185746016167 1101 19 0.895742881534261 1101 20 1.051314234527581 1101 21 0.961902138673949 1101 22 1.0603844825990052 1101 23 1.3491429373793054 1101 24 1.0373838244402904 1101 25 1.2197612684666055 1102 1 0.8484920746181999 1102 2 0.81064235744293 1102 3 0.860246555347608 1102 4 0.9856577978656915 1102 5 0.868118536867506 1102 6 0.9301957612206522 1102 7 1.0172336474828967 1102 8 1.058283726393185 1102 9 1.1327030235607012 1102 10 1.2500264707108002 1102 11 1.2200208885509554 1102 12 1.2176133766124266 1102 13 1.0962055807319402 1102 14 1.1877388874143264 1102 15 1.3580634975586534 1102 16 1.366566648027245 1102 17 1.3395110713345346 1102 18 1.5402325431409523 1102 19 1.559198269238756 1102 20 1.4710853304398626 1102 21 1.315205722250738 1102 22 1.467469890957112 1102 23 1.5885549876686724 1102 24 1.4887969597156356 1102 25 1.338359976747988 1103 1 0.743157358158129 1103 2 0.8686067677528939 1103 3 0.8113838453723616 1103 4 0.8809077820672258 1103 5 0.595175019371311 1103 6 1.066753807929942 1103 7 0.8023432426412 1103 8 0.7854512705307357 1103 9 1.154332959058877 1103 10 1.0576432623060583 1103 11 1.139478424901597 1103 12 1.3282014763058854 1103 13 1.2171575783822206 1103 14 1.331019837026986 1103 15 1.352786810277351 1103 16 1.458369060773009 1103 17 1.4075062996732655 1103 18 1.6463517242409083 1103 19 1.7600804219281372 1103 20 1.5597069505918597 1103 21 1.298377470354552 1103 22 1.414773875046535 1103 23 1.7593209623310813 1103 24 1.476939923121357 1103 25 1.41488364683741 1104 1 0.6735541977698283 1104 2 0.577428024144826 1104 3 0.6468574811555001 1104 4 0.9215785898953115 1104 5 0.5695630742504818 1104 6 0.6140869152615522 1104 7 0.7616548752640284 1104 8 0.7591931340037945 1104 9 0.8383267690079492 1104 10 0.9265607119736785 1104 11 0.8617182755338746 1104 12 0.9655768525708694 1104 13 0.8914909507597315 1104 14 1.127397136785632 1104 15 1.2544117089742457 1104 16 1.0812626637466423 1104 17 1.052754964579428 1104 18 1.2372184656492777 1104 19 1.0626640926630415 1104 20 1.08176856147184 1104 21 1.2184618966665255 1104 22 1.1172703884431243 1104 23 1.4096917477036044 1104 24 1.27918645051394 1104 25 0.9956329892322984 1105 1 0.6594298017429132 1105 2 0.7510036729753296 1105 3 0.8699405442762966 1105 4 0.9866336363547816 1105 5 0.6799665603126428 1105 6 0.4694015964534799 1105 7 0.7715266855812359 1105 8 0.810648949447561 1105 9 0.7119772143780239 1105 10 0.9676138103395857 1105 11 0.9203717840247378 1105 12 0.9886935459383821 1105 13 0.9598383778664257 1105 14 1.121526813171407 1105 15 1.229878854120031 1105 16 1.2420519492320636 1105 17 1.065474887680999 1105 18 1.122093826110671 1105 19 1.1612149673826835 1105 20 1.1721726114558209 1105 21 1.1030161292842777 1105 22 0.8625769440342808 1105 23 1.0487115490493295 1105 24 1.1333889753457242 1105 25 1.1939814160185804 1106 1 0.5783617550404827 1106 2 0.6970515114101694 1106 3 0.7142651387856027 1106 4 0.7908005028741911 1106 5 0.6645869705366891 1106 6 0.5264569198993598 1106 7 0.42343868305678 1106 8 0.6621887411799676 1106 9 0.831350341547477 1106 10 0.8986311407277228 1106 11 0.7968733933652246 1106 12 0.892959623565396 1106 13 0.8968644176266025 1106 14 1.0973759517009114 1106 15 1.151230245726218 1106 16 1.1082737731512822 1106 17 0.9331579675169124 1106 18 1.2239101719610943 1106 19 0.9009909471870401 1106 20 0.9448647667098413 1106 21 0.8747521217109124 1106 22 0.9588594811122277 1106 23 1.2040251495542194 1106 24 1.1585157913760185 1106 25 1.2111792197421565 1107 1 0.4867401431146625 1107 2 0.6083175470749254 1107 3 0.8276975376994073 1107 4 0.9016432198186095 1107 5 0.7910463473467786 1107 6 0.6283214335972289 1107 7 0.7620586743065729 1107 8 0.8206994348844532 1107 9 0.9290995695299173 1107 10 0.8489649659925789 1107 11 0.8843489841500158 1107 12 0.9772630417103798 1107 13 1.0006493518720632 1107 14 1.220975056203038 1107 15 1.3364746323849843 1107 16 1.1866172859060384 1107 17 1.3006396105121922 1107 18 1.5777255204420229 1107 19 1.376627983355121 1107 20 1.3887612992102802 1107 21 1.3434777390556505 1107 22 1.4688186978708018 1107 23 1.388665698751801 1107 24 1.3314545437766654 1107 25 1.4294253496233438 1108 1 0.3812540106331852 1108 2 0.4985264581789366 1108 3 0.3789773860543484 1108 4 0.517204478754536 1108 5 0.49193219288957835 1108 6 0.44175278078670743 1108 7 0.5525736734581415 1108 8 0.6436153156272368 1108 9 0.7316383645179059 1108 10 0.7067852620026258 1108 11 0.6503452997225949 1108 12 0.7068737137966323 1108 13 0.7137032706236539 1108 14 0.8444396387853317 1108 15 0.8406833552080002 1108 16 0.8146156048117987 1108 17 0.8426195068914453 1108 18 0.9899856507920504 1108 19 0.9331383245477411 1108 20 0.8505916105232743 1108 21 0.6541371986035216 1108 22 0.6307897136954566 1108 23 0.9072003274142659 1108 24 0.8832241758430825 1108 25 0.9667651026831763 1109 1 -0.1178351790999983 1109 2 0.0323819616087917 1109 3 0.13643924159430298 1109 4 0.20368804248504802 1109 5 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96292.0 3 0.0 4 820.0 5 4675.0 6 5534.0 7 5190.0 8 4548.0 9 4350.0 10 4134.0 11 3904.0 12 3603.0 13 3678.0 14 3579.0 15 3765.0 16 3722.0 17 4069.0 18 4488.0 19 5441.0 20 7019.0 21 8948.0 22 11180.0 23 13683.0 24 15603.0 25 18087.0 26 20206.0 27 22663.0 28 25339.0 29 30117.0 30 36784.0 31 46547.0 32 58634.0 33 75334.0 34 98389.0 35 129725.0 36 178258.0 37 281318.0 38 766915.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.842452170059566 18.889043941374155 12.525689946046736 25.742813942519543 2 14.181093902296144 20.433253313590143 42.193438381058506 23.192214403055207 3 18.451236967384624 25.171088520330454 29.971189027202165 26.406485485082754 4 11.872211907957317 17.13835317859942 39.24641498974002 31.74301992370324 5 12.513143587051303 38.28010834952444 36.67854762178976 12.528200441634496 6 30.866541520161185 38.01315134917969 18.611621478011468 12.508685652647667 7 27.127521702894654 31.669627511733715 23.253051564562373 17.949799220809258 8 24.175948524075004 37.93886934813645 21.227288860175946 16.657893267612597 9 26.37014690568895 15.07454450562953 20.516101503551386 38.03920708513013 10 14.408922674309926 29.224549816187924 35.55655804099944 20.809969468502707 11 33.81074742480018 22.272057742298077 25.814655398012615 18.102539434889128 12 23.08622528836472 24.65156968107909 32.822435124168805 19.43976990638739 13 28.899158482616848 22.351403542527983 26.00823073985937 22.741207234995798 14 20.295784421442203 22.65611758395364 28.162331978057697 28.88576601654646 15 22.29122724981842 32.07171781474626 23.46118317644355 22.17587175899177 16 21.48610993415117 28.28927626102258 28.837900214843653 21.3867135899826 17 21.14871560712313 29.06608098165381 28.526721712550422 21.25848169867264 18 21.041425018645874 27.72460481973993 30.75244516616653 20.481524995447664 19 23.23290632162765 27.014497261761267 27.709849384407242 22.04274703220384 20 23.17552687172933 27.903888467393724 28.513682863047745 20.406901797829207 21 22.404484904921095 27.170992483616796 27.352596038520094 23.07192657294202 22 22.814094949647007 28.80541059758132 27.56696078084398 20.81353367192769 23 21.79409978974108 28.36556339720193 28.190746414183153 21.649590398873833 24 22.863038997485237 27.19889539940832 28.88106851165538 21.05699709145106 25 22.357100272569753 27.75241614043623 28.480299009217923 21.410184577776096 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 203.0 1 203.0 2 328.5 3 454.0 4 454.0 5 454.0 6 973.5 7 1493.0 8 1493.0 9 1493.0 10 2339.5 11 3186.0 12 3186.0 13 3186.0 14 5913.0 15 8640.0 16 8640.0 17 8640.0 18 16149.0 19 23658.0 20 23658.0 21 23658.0 22 38685.0 23 53712.0 24 53712.0 25 53712.0 26 78997.0 27 104282.0 28 104282.0 29 104282.0 30 136495.0 31 168708.0 32 168708.0 33 168708.0 34 190271.0 35 211834.0 36 211834.0 37 211834.0 38 226296.0 39 240758.0 40 240758.0 41 240758.0 42 248545.5 43 256333.0 44 256333.0 45 256333.0 46 258013.0 47 259693.0 48 259693.0 49 259693.0 50 245896.0 51 232099.0 52 232099.0 53 232099.0 54 203274.5 55 174450.0 56 174450.0 57 174450.0 58 148339.5 59 122229.0 60 122229.0 61 122229.0 62 98824.0 63 75419.0 64 75419.0 65 75419.0 66 57938.5 67 40458.0 68 40458.0 69 40458.0 70 29217.0 71 17976.0 72 17976.0 73 17976.0 74 12497.0 75 7018.0 76 7018.0 77 7018.0 78 4786.0 79 2554.0 80 2554.0 81 2554.0 82 1740.0 83 926.0 84 926.0 85 926.0 86 632.5 87 339.0 88 339.0 89 339.0 90 209.0 91 79.0 92 79.0 93 79.0 94 53.0 95 27.0 96 27.0 97 27.0 98 20.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3597235242140579 2 0.039171888339186695 3 0.0352846017101071 4 0.03433769855686976 5 0.04036797653274964 6 0.017891485895379162 7 0.017791811879248918 8 0.023772252847063677 9 0.02113089141961216 10 0.020732195355091174 11 0.019237085113137483 12 0.019984640234114327 13 0.020084314250244574 14 0.027559865460013027 15 0.02830742058098987 16 0.02147975047606802 17 0.022775512685761216 18 0.10346162874319538 19 0.025267363089017367 20 0.022675838669630972 21 0.026064755218059336 22 0.0201341512583097 23 0.022077794572849495 24 0.019237085113137483 25 0.022376816621240234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2006541.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.25096836728898 #Duplication Level Percentage of deduplicated Percentage of total 1 83.86610269014962 59.75541029863659 2 11.02113090339436 15.705324987390048 3 2.6966387281911377 5.764143621610594 4 0.9722665980715667 2.7709974649507547 5 0.4470638503041571 1.5926866128089956 6 0.2523267461775996 1.0787115006072663 7 0.143178663797263 0.7141132911562641 8 0.1052470857227264 0.5999165420463465 9 0.08011406097651971 0.5137383981971759 >10 0.3732882636568605 4.666207707730606 >50 0.0235047131348771 1.167609496665941 >100 0.015423766346887169 2.316193704268357 >500 0.0022423728763511903 1.160288693857951 >1k 0.0014715572001054453 2.1946576800731323 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4635 0.2309945323818452 No Hit CTGTAGGACGTGGAATATGGCAAGA 4313 0.21494701578487554 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4303 0.2144486457042243 No Hit CTTTAGGACGTGAAATATGGCGAGG 3752 0.18698845426034155 No Hit GTATCAACGCAGAGTACTTTTTTTT 2871 0.14308205015496817 No Hit GTCCTACAGTGTGCATTTCTCATTT 2856 0.14233449503399134 No Hit CTGTAGGACCTGGAATATGGCGAGA 2079 0.10361113976739074 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.9837008065123016E-5 0.0 8 0.0 0.0 0.0 4.9837008065123016E-5 0.0 9 0.0 0.0 0.0 1.9934803226049206E-4 0.0 10 0.0 0.0 0.0 1.9934803226049206E-4 0.0 11 0.0 0.0 0.0 1.9934803226049206E-4 0.0 12 0.0 0.0 0.0 1.9934803226049206E-4 0.0 13 0.0 0.0 0.0 1.9934803226049206E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACCGT 55 1.9704885E-4 13.809987 6 TAGGACC 755 0.0 13.46096 4 GCACCGT 85 3.971174E-6 12.286826 6 CTACACG 55 0.0030734485 12.088561 4 TATTCCG 55 0.0030740225 12.088259 5 GTCCTAT 270 0.0 11.998037 1 TCGTTGG 65 8.008076E-4 11.695286 12 GGCGAGG 980 0.0 11.432563 19 CCAACGA 150 1.7826096E-10 11.393807 19 AGGACCT 1475 0.0 11.204324 5 CGTAAAC 60 0.005885167 11.081182 3 ATTATAC 200 0.0 10.922878 3 GGACCTG 1445 0.0 10.709908 6 ACGACAG 80 3.779349E-4 10.685158 5 TGTAGGA 2340 0.0 10.634952 2 TCCAACG 200 1.8189894E-12 10.452401 18 GTCCTAA 1320 0.0 10.394022 1 GTAGGAC 2265 0.0 10.273942 3 AATCCCG 250 0.0 10.254428 19 GTTAGGT 75 0.002652415 10.131365 3 >>END_MODULE