FastQCFastQC Report
Thu 26 May 2016
SRR1512947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006541
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT42860.21360141656711723No Hit
CTGTAGGACGTGGAATATGGCAAGA42430.21145842522031694No Hit
GTCCTAAAGTGTGTATTTCTCATTT39890.19879982517177572No Hit
CTTTAGGACGTGAAATATGGCGAGG38210.19042720781683503No Hit
GTATCAACGCAGAGTACTTTTTTTT34230.17059207860691608No Hit
GTCCTACAGTGTGCATTTCTCATTT27650.13779932730006514No Hit
TATCAACGCAGAGTACTTTTTTTTT23630.11776485005788569No Hit
CTGAAGGACCTGGAATATGGCGAGA21450.10690038229968887No Hit
GGTATCAACGCAGAGTACTTTTTTT21240.10585380513032129No Hit
CTGTAGGACCTGGAATATGGCGAGA20610.10271407362221854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC400.005308806414.2382415
AGTTACG500.001509171713.28902314
CCAACGA1153.8380676E-1013.20187619
TAGGACC9450.013.0899764
TCCAACG1850.012.3095518
CAAGTCG2250.011.414786
GGCGAGG9450.011.04521919
ATTAGAC1657.8216544E-1110.94837953
TGTAGGA27000.010.7045482
AGGACGT22800.010.6004965
GGTATCA9350.010.5744681
GGACGTG22350.010.5550536
AGGACCT18250.010.5321215
GTAGGAC26600.010.5086243
TGGCGAG24500.010.41810818
GACGTGG12500.010.417697
GATATAC6250.010.3434561
TTAGACT1855.820766E-1110.2869254
TCCCGTT1856.002665E-1110.26256211
GTATTAA2700.010.2110641
Produced by FastQC (version 0.11.5)