FastQCFastQC Report
Thu 26 May 2016
SRR1512946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544310
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT28590.18513122365328205No Hit
GTCCTAAAGTGTGTATTTCTCATTT28040.181569762547675No Hit
GTATCAACGCAGAGTACTTTTTTTT27490.17800830144206797No Hit
CTGTAGGACGTGGAATATGGCAAGA26180.16952554862689487No Hit
CTTTAGGACGTGAAATATGGCGAGG24230.15689855016156082No Hit
GGTATCAACGCAGAGTACTTTTTTT16820.1089159559932915No Hit
TATCAACGCAGAGTACTTTTTTTTT15860.10269958751805014No Hit
GTCCTACAGTGTGCATTTCTCATTT15540.10062746469296968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG250.0060392518.9960825
ACGATGC500.001505174213.2933828
GGTATCA7700.012.2466741
TAAGACG550.003072427612.0888074
GTCCTAG1353.5470293E-1011.9946831
TAGGACC4700.011.9234584
GGCGAGG7800.011.68611119
TCCAACG1157.054405E-811.567327518
GTCCTAC16250.011.1371461
CCTCGAA1201.2717464E-711.08535617
GGCGTGC600.00589743611.0778188
ACCGATC600.00589743611.0778188
GTCTAAT1158.518891E-710.7676061
GCCTCGA1158.793777E-710.7410916
TCCTACA17050.010.6964342
GGACGTG15500.010.6588646
CTATACT2050.010.6566834
TGGCGAG15800.010.64434618
CGAAATC1002.3918063E-510.45258513
AGGACGT16000.010.3884825
Produced by FastQC (version 0.11.5)