FastQCFastQC Report
Thu 26 May 2016
SRR1512946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544310
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33710.21828518885456935No Hit
GTCCTACAGTGGACATTTCTAAATT27180.1760009324552713No Hit
CTTTAGGACGTGAAATATGGCGAGG26710.17295750205593435No Hit
CTGTAGGACGTGGAATATGGCAAGA26500.17159767145197533No Hit
TATCAACGCAGAGTACTTTTTTTTT24750.16026574975231656No Hit
GTCCTAAAGTGTGTATTTCTCATTT24670.15974771904604645No Hit
GGTATCAACGCAGAGTACTTTTTTT22250.1440772901813755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGT604.0452325E-412.6839354
GGTATCA7450.012.5067791
TAGGACC5550.011.8269124
GGCGAGG7950.011.57843519
AGGACGT16550.011.2086135
GGACGTG16050.011.1985336
TCCAACG1451.2387318E-911.125669518
TTAGGAC10450.011.0991853
GTACCGT600.00583040711.0948426
GGACCTG10200.010.9083746
GACGTGA9050.010.8167137
AGGACCT11200.010.7870075
GCTAATT803.7469857E-410.694782
TGTAGGA17000.010.68082
GACGTGG8150.010.6118347
GAATAAC909.436167E-510.56411651
TGTACCG1002.3625491E-510.4642475
GTCCTAT2001.8189894E-1210.4584751
TAGGACG17900.010.4164154
ATAGGAC1659.1677066E-1010.3713683
Produced by FastQC (version 0.11.5)