FastQCFastQC Report
Thu 26 May 2016
SRR1512945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991939
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT41740.20954456938691396No Hit
GTCCTAAAGTGTGTATTTCTCATTT38730.19443366488632433No Hit
CTGTAGGACGTGGAATATGGCAAGA35690.17917215336413414No Hit
CTTTAGGACGTGAAATATGGCGAGG33040.16586853312275124No Hit
GTCCTACAGTGTGCATTTCTCATTT29670.1489503443629549No Hit
GTATCAACGCAGAGTACTTTTTTTT29470.14794629755228447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT1302.0008883E-1012.41595411
ACGAAAT1603.6379788E-1211.87862812
TACACGG658.038429E-411.6900055
CGAAATC1552.5465852E-1111.64871913
GTATTAC1156.798109E-811.5987681
TAGGACC7450.011.2192674
GGCGTAG600.005751269411.1154871
GAGCGTG600.005903992311.0764217
GGCGAGG9000.010.97120819
TAGGACA8150.010.7218134
GGTATCA9900.010.6824171
TCCAACG2150.010.60762418
ACCTTTT7400.010.52997915
TTAGGAC13500.010.483123
GTGCTAT2001.8189894E-1210.4803161
TTATACT2100.010.4027144
AGGACGT18800.010.3570015
GACGTGA9950.010.3051277
CCAACGT1409.775795E-810.17247919
TGTAGGA21750.010.1313382
Produced by FastQC (version 0.11.5)