##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512945_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1991939
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.081661135205447	31.0	30.0	34.0	23.0	34.0
2	29.043099713394838	31.0	30.0	34.0	22.0	34.0
3	29.301018756096447	31.0	30.0	34.0	23.0	34.0
4	32.7165435286924	35.0	33.0	37.0	25.0	37.0
5	32.89187118681847	35.0	35.0	37.0	26.0	37.0
6	32.87973125683066	37.0	35.0	37.0	26.0	37.0
7	32.74046996419067	37.0	33.0	37.0	25.0	37.0
8	32.73240696627758	37.0	35.0	37.0	25.0	37.0
9	34.06568775449449	39.0	35.0	39.0	25.0	39.0
10	33.931198194322214	38.0	34.0	39.0	23.0	39.0
11	34.007469104224576	39.0	35.0	39.0	25.0	39.0
12	33.89748983277099	38.0	34.0	39.0	23.0	39.0
13	33.89787137055904	39.0	34.0	39.0	23.0	39.0
14	34.87795409397577	39.0	35.0	41.0	22.0	41.0
15	34.834507482407844	39.0	34.0	41.0	22.0	41.0
16	34.71468754816287	39.0	34.0	41.0	19.0	41.0
17	34.6609615053473	39.0	34.0	41.0	19.0	41.0
18	34.34750562140708	39.0	34.0	41.0	17.0	41.0
19	34.59832002887639	39.0	34.0	41.0	18.0	41.0
20	34.686113379978	39.0	34.0	41.0	19.0	41.0
21	34.62601565610192	39.0	34.0	41.0	18.0	41.0
22	34.53073864209697	39.0	34.0	41.0	18.0	41.0
23	34.25638485917491	39.0	34.0	41.0	16.0	41.0
24	34.27205351167882	39.0	34.0	41.0	15.0	41.0
25	34.187270794939	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3274889271918262
1101	2	0.6741981318645145
1101	3	0.6338458431228382
1101	4	0.5623908843209264
1101	5	0.4266550453056155
1101	6	0.6029385353783283
1101	7	0.6566294626002218
1101	8	0.4171626560556092
1101	9	0.38885052982892887
1101	10	0.4316343001658396
1101	11	0.5157515472109395
1101	12	0.8273688996010335
1101	13	0.5620884134375785
1101	14	0.6567841966447929
1101	15	0.40757034558651384
1101	16	0.8794047562762586
1101	17	0.8830446051420537
1101	18	0.9882365779063491
1101	19	0.6495635202537144
1101	20	0.7561537246164534
1101	21	0.8477327413282012
1101	22	0.7899439727707076
1101	23	1.0922002856327637
1101	24	0.744284524559788
1101	25	0.9188243032887087
1102	1	0.5632887238165765
1102	2	0.5720191050035801
1102	3	0.6430037032045597
1102	4	0.6601269407703896
1102	5	0.5768250556867827
1102	6	0.5997140021988727
1102	7	0.7130809184855806
1102	8	0.6976784574629491
1102	9	0.6779630951094617
1102	10	0.8264975705779207
1102	11	0.7643923934219004
1102	12	0.8118252513605668
1102	13	0.6481045064556454
1102	14	0.7753759513625553
1102	15	1.0297092195531548
1102	16	1.0246968139328771
1102	17	0.7374104912005848
1102	18	0.9777529731552619
1102	19	1.0100934995179358
1102	20	1.0340131691312067
1102	21	0.8090336968732714
1102	22	1.1301191693092534
1102	23	1.270706982059366
1102	24	1.135344244135581
1102	25	0.9673097860305759
1103	1	0.3521192073809658
1103	2	0.5710924854532813
1103	3	0.47861019524694015
1103	4	0.6242043203706018
1103	5	0.22526306251439365
1103	6	0.49871088634817795
1103	7	0.3737043422021813
1103	8	0.32259395757864695
1103	9	0.7837118343468887
1103	10	0.6063955262037268
1103	11	0.6799657300235822
1103	12	0.7029081494715115
1103	13	0.6428644042210863
1103	14	0.874006308348811
1103	15	0.9160077178378003
1103	16	0.9620938899568898
1103	17	0.8629631082932789
1103	18	1.0990155636116086
1103	19	1.1021042850736649
1103	20	1.0238831148547547
1103	21	0.8441295444131853
1103	22	0.9109346579082427
1103	23	1.282990026823434
1103	24	1.061597795560857
1103	25	0.9664417009421769
1104	1	0.5789539920454523
1104	2	0.5894152457406605
1104	3	0.5821945234973285
1104	4	0.812154462876876
1104	5	0.6048414540979863
1104	6	0.5492786786173767
1104	7	0.7379519484908457
1104	8	0.7535161110651245
1104	9	0.8354490974384206
1104	10	1.025858862032564
1104	11	0.938700379405617
1104	12	0.8033728381248579
1104	13	0.8828071390280812
1104	14	1.0144305436089383
1104	15	1.130632932910629
1104	16	1.0722568856094625
1104	17	1.1003105215089306
1104	18	1.3339053018466274
1104	19	1.1199431807485638
1104	20	1.211213847612811
1104	21	1.3504757361465138
1104	22	1.330528810554135
1104	23	1.5546997080559493
1104	24	1.4244484463613887
1104	25	1.16646008884819
1105	1	0.6243782195312306
1105	2	0.7249016812594888
1105	3	0.8003702240223802
1105	4	0.8463277220300611
1105	5	0.6316076536175359
1105	6	0.4392801653502474
1105	7	0.7428838326508824
1105	8	0.847395310107359
1105	9	0.6333103930869868
1105	10	0.8409368372589014
1105	11	0.7460645585334547
1105	12	0.7885283582074933
1105	13	0.7763561268759958
1105	14	0.9127342596586061
1105	15	1.1083497807239624
1105	16	1.1683414572347743
1105	17	1.0400796866693014
1105	18	1.1720939917219795
1105	19	1.1238431158018116
1105	20	1.0648050899737385
1105	21	0.9420069927487802
1105	22	0.6690106215512586
1105	23	1.0043381189732656
1105	24	1.0046705173020172
1105	25	0.9926328472071475
1106	1	0.5741583891598445
1106	2	0.5935327519605345
1106	3	0.661866717786495
1106	4	0.5479966705947277
1106	5	0.5315386215818236
1106	6	0.6050267814249111
1106	7	0.38139031706146653
1106	8	0.5275958295831842
1106	9	0.6563803612965131
1106	10	0.8616029050043679
1106	11	0.568975615448629
1106	12	0.8785493368808872
1106	13	0.7197867253412298
1106	14	0.9673268720600561
1106	15	0.9289045879703295
1106	16	0.8340723608704934
1106	17	0.7433335376545855
1106	18	1.0457298336642893
1106	19	0.549578940614488
1106	20	0.784651071101564
1106	21	0.6251567267464324
1106	22	0.7166435477578403
1106	23	0.9368838996801969
1106	24	0.8888031927257529
1106	25	0.8853175053352871
1107	1	0.48024384141118404
1107	2	0.45475948094939156
1107	3	0.6142006384302725
1107	4	0.4614219834723059
1107	5	0.5446516749922168
1107	6	0.47782228997942156
1107	7	0.641638357434708
1107	8	0.559544261375116
1107	9	0.7087847470342297
1107	10	0.5574182471420883
1107	11	0.5253126911127808
1107	12	0.7471713541726999
1107	13	0.7100893138441293
1107	14	0.9394148316284543
1107	15	0.9840950504377872
1107	16	0.8725073507921763
1107	17	0.9238372627592497
1107	18	1.2311095730346437
1107	19	1.0453348221293624
1107	20	1.000162187238658
1107	21	0.9685845850412704
1107	22	0.9475264711290308
1107	23	1.0517069425157572
1107	24	1.0154913049381236
1107	25	1.0624274758724894
1108	1	0.5444179071527024
1108	2	0.5596109255108601
1108	3	0.45628695983689127
1108	4	0.6080658061518989
1108	5	0.5190609897527807
1108	6	0.7292350436186723
1108	7	0.7752838012579133
1108	8	0.7710380914788999
1108	9	0.931351943097205
1108	10	0.727261281507225
1108	11	0.6106066866700388
1108	12	0.7451285081983912
1108	13	0.7839388859659451
1108	14	0.8801732118083052
1108	15	0.8772237987551676
1108	16	0.7788616187295716
1108	17	0.8299358704716226
1108	18	0.9684791307525984
1108	19	0.9127405386676415
1108	20	0.8738829853654266
1108	21	0.7271633861906892
1108	22	0.6788402612223408
1108	23	0.963461740915136
1108	24	0.8717552494069736
1108	25	0.9283949387192934
1109	1	0.2628469407025662
1109	2	0.20299532862319936
1109	3	0.48299962298801447
1109	4	0.5816688724178576
1109	5	0.4669011801427132
1109	6	0.3058194397448375
1109	7	0.5147982994446281
1109	8	0.5737603298497262
1109	9	0.83669518266192
1109	10	0.7931553727408627
1109	11	0.4531450347471164
1109	12	0.5905738124692306
1109	13	0.6801384477497265
1109	14	0.5040784509829521
1109	15	0.780869875746987
1109	16	0.7207071086330927
1109	17	0.5562068866982628
1109	18	-0.9829859701789303
1109	19	0.5043805601418114
1109	20	0.766011228271438
1109	21	0.6962560025094859
1109	22	0.8537163085769706
1109	23	0.35619981877690066
1109	24	0.8050222972360856
1109	25	0.8350153386030215
1110	1	0.2888060139580304
1110	2	0.18695970357107328
1110	3	0.2641848401386113
1110	4	0.17062573482164822
1110	5	0.3185646979473873
1110	6	0.3199364347755562
1110	7	0.4138470530150471
1110	8	0.24283910942092746
1110	9	0.6874176681958915
1110	10	0.8918045048999872
1110	11	0.6773636515928629
1110	12	0.19903760297381012
1110	13	0.3837441062728715
1110	14	0.6963772169433327
1110	15	0.731220602161045
1110	16	0.703859185975432
1110	17	0.40591792874796084
1110	18	0.807132103080157
1110	19	0.7678705025194503
1110	20	0.6569802195356189
1110	21	0.6565277595744803
1110	22	0.7608375717964506
1110	23	0.6659831698188086
1110	24	0.7975568954328267
1110	25	0.9117816930427978
1111	1	0.38488839725123825
1111	2	0.7420239172996226
1111	3	0.6574045044189489
1111	4	0.5673600106480237
1111	5	0.6133002218404826
1111	6	0.7756492741636478
1111	7	0.7724200100166385
1111	8	0.5510132809735353
1111	9	0.8895101007553663
1111	10	0.7260303299595137
1111	11	0.5876341275551766
1111	12	0.7260662936864861
1111	13	0.7647653249195798
1111	14	0.6430303805323305
1111	15	0.9528401668622308
1111	16	0.79871376926301
1111	17	0.7810385081461462
1111	18	0.967727073365225
1111	19	0.8160602159208636
1111	20	0.6872487111746892
1111	21	0.8513707575602112
1111	22	0.7742181417025549
1111	23	0.9406839862044762
1111	24	0.9888084498445764
1111	25	0.3261105032685947
1112	1	-0.2822072644263329
1112	2	-0.029265062796053343
1112	3	0.01666082133968061
1112	4	0.1620328331569354
1112	5	0.07648997532852775
1112	6	0.23716812391486997
1112	7	-0.16720125248754414
1112	8	0.10918600044355031
1112	9	0.28595673241037645
1112	10	0.2641607269873134
1112	11	0.23519829737436027
1112	12	0.31652237658006754
1112	13	-0.059672420028817896
1112	14	0.18661151957876854
1112	15	0.16769725597398377
1112	16	0.3428661144477161
1112	17	0.27619218908865406
1112	18	0.258571520985889
1112	19	0.20627379033748383
1112	20	0.05351341834657575
1112	21	0.11785685203296481
1112	22	0.044679781611456804
1112	23	0.2533557369446129
1112	24	0.12065284807639642
1112	25	0.3574298087320429
1113	1	-0.26510444627264107
1113	2	-0.18106495877810147
1113	3	-0.26411546368186123
1113	4	0.0030266616838474647
1113	5	-0.15938741651933697
1113	6	0.08141990711181535
1113	7	-0.13100188892288145
1113	8	0.21322944700868618
1113	9	0.10096787953842323
1113	10	-0.28768190736946053
1113	11	0.02343532647180524
1113	12	0.14486327625870388
1113	13	-0.08467038617209965
1113	14	0.014799967133200198
1113	15	-0.4111523070380869
1113	16	-0.326033263864268
1113	17	-0.27913872114213945
1113	18	-0.3454516616946748
1113	19	-0.2582368786724274
1113	20	-0.487785822270574
1113	21	-0.22856784692388032
1113	22	-0.2350625972013063
1113	23	-0.6572302458044987
1113	24	-0.04124752391276587
1113	25	-0.057018111939484584
1114	1	-1.5446334256124068
1114	2	-1.3350894672381592
1114	3	-1.3041216394745483
1114	4	-1.4881522278560695
1114	5	-1.4905439919765904
1114	6	-2.021248465363737
1114	7	-2.1727553840094274
1114	8	-1.6108775267991469
1114	9	-1.7287038776776669
1114	10	-1.887072749636495
1114	11	-2.575122918599657
1114	12	-2.047237920734151
1114	13	-1.9976353101781932
1114	14	-2.3143539552417423
1114	15	-2.2285710287684566
1114	16	-2.804846830778743
1114	17	-4.890776626174691
1114	18	-3.9288490524521293
1114	19	-2.8613406624503988
1114	20	-1.9631632832552697
1114	21	-2.9623849108660316
1114	22	-3.191735887614673
1114	23	-3.0659028744404644
1114	24	-2.498384894685497
1114	25	-2.420414535116265
1115	1	-1.1630259582163696
1115	2	-0.7670578745320249
1115	3	-0.8519451244222651
1115	4	-0.702864098835029
1115	5	-0.7104371260469051
1115	6	-0.8887534194996753
1115	7	-1.814228962551958
1115	8	-1.2294705481871553
1115	9	-0.8799487307640561
1115	10	-0.7454653783789311
1115	11	-1.5726842058778558
1115	12	-1.1652286746160456
1115	13	-0.924613529605459
1115	14	-1.0587467786646059
1115	15	-2.3057033301353442
1115	16	-1.1244533920696256
1115	17	-1.247599393689022
1115	18	-1.2218364715305725
1115	19	-1.2996189585080415
1115	20	-1.0718205932445812
1115	21	-1.6604289735338966
1115	22	-1.831621979541481
1115	23	-1.2692892321501077
1115	24	-1.18295955602769
1115	25	-1.553389872792657
1116	1	-0.6115440608342375
1116	2	-1.4317172173370878
1116	3	-1.1114472393474202
1116	4	-0.5315914055293618
1116	5	-1.044868280090057
1116	6	-0.8618883920796989
1116	7	-0.5769455361548239
1116	8	-1.1619971960239006
1116	9	-1.241567532594587
1116	10	-2.2455678718232974
1116	11	-0.8471977106966975
1116	12	-0.7941462502506056
1116	13	-1.3506637295548458
1116	14	-1.0847072578531112
1116	15	-0.7022221132289346
1116	16	-0.6678022465379314
1116	17	-0.9510069474466931
1116	18	-0.821671142355946
1116	19	-1.22522459199552
1116	20	-1.56375746976191
1116	21	-1.9711294542896312
1116	22	-1.0983049668592741
1116	23	-1.1615273311196859
1116	24	-1.758289301386995
1116	25	-1.4132761959096172
1201	1	0.2438160952361983
1201	2	0.19979864497158317
1201	3	0.38997803228638617
1201	4	0.42230019688924614
1201	5	0.4302954966304071
1201	6	0.4267814040115425
1201	7	0.41339671654759513
1201	8	-0.011206820753919544
1201	9	0.19228308432398933
1201	10	0.4785173823469222
1201	11	0.028532733766517993
1201	12	-0.5729906996265939
1201	13	-0.005402453274648167
1201	14	0.30355999034036074
1201	15	0.06664707784194235
1201	16	0.5146934151115161
1201	17	0.6543540301895163
1201	18	0.8632039670814464
1201	19	0.3703480577126115
1201	20	0.3057722795213422
1201	21	0.3363645400645723
1201	22	0.46664589674167445
1201	23	0.5184670976978225
1201	24	0.34906073531282544
1201	25	0.32695678854501153
1202	1	0.3550300934499333
1202	2	0.5911972442566409
1202	3	0.2514363386994596
1202	4	0.5920373427299666
1202	5	0.47129021536826343
1202	6	0.3586469024375063
1202	7	0.32316278076988425
1202	8	0.5664087224600536
1202	9	0.7196526110428252
1202	10	0.8304867626779568
1202	11	0.4929473993544775
1202	12	0.5667380405227789
1202	13	0.5901784483288282
1202	14	0.7480283314581229
1202	15	0.34302108423797506
1202	16	0.6336928618864945
1202	17	0.7564516422110117
1202	18	1.0969638779346909
1202	19	0.7432545740726084
1202	20	0.8076660787142416
1202	21	0.8044334255592531
1202	22	0.9461340825622031
1202	23	0.9766022431679744
1202	24	0.9193049821887271
1202	25	0.7758601283508852
1203	1	0.42959553330129197
1203	2	0.6271728785031563
1203	3	0.7759904714302124
1203	4	0.8629736334356011
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2211	14	-0.06770998275259643
2211	15	-0.12448822821351513
2211	16	-0.2930079938614867
2211	17	-0.13887125573158698
2211	18	-0.13463705121538538
2211	19	-0.3470237221566137
2211	20	-0.5087968976841495
2211	21	-0.4086706448216759
2211	22	-0.5295250739497277
2211	23	0.11817075501132024
2211	24	-0.03535513593099893
2211	25	-0.04514799224719468
2212	1	-0.031262750449119636
2212	2	-0.07308444097373012
2212	3	-0.07822893635224304
2212	4	-0.16289102107906928
2212	5	-0.13908262081435652
2212	6	-0.25199154748532493
2212	7	-0.37202004761162755
2212	8	-0.36930903464465104
2212	9	-0.2797630800372417
2212	10	-0.26984510991750454
2212	11	-0.33801413684303583
2212	12	-0.2442769571525858
2212	13	-0.3478434976450018
2212	14	-0.31415946654407634
2212	15	-0.3868814462595864
2212	16	-0.17749563154452375
2212	17	-0.15702488846045526
2212	18	-3.063614499082739
2212	19	-1.3786095675974153
2212	20	-0.5533350083192872
2212	21	-0.6695103002662322
2212	22	-0.3739531940645904
2212	23	-0.49591231137031855
2212	24	-0.7048118159801504
2212	25	-0.47578462173984093
2213	1	0.10409133290260897
2213	2	0.2936749108994512
2213	3	-0.06484802340997575
2213	4	-0.09852843286221713
2213	5	-0.012703043717934293
2213	6	0.0678261455452045
2213	7	0.2604372972449056
2213	8	-0.23041244370799063
2213	9	-0.09160619644147516
2213	10	-0.08331989094043024
2213	11	-0.025343209022615554
2213	12	-0.019414085346667775
2213	13	0.1494700748734985
2213	14	0.137922903197925
2213	15	-0.047405587818744266
2213	16	-0.5976782913713663
2213	17	-0.0660722918411949
2213	18	0.09456534630558622
2213	19	0.17323640775349247
2213	20	-0.6157568809854865
2213	21	-0.22482560539462781
2213	22	-0.06285177984833012
2213	23	-0.6862867832621191
2213	24	-0.2257018746446917
2213	25	-0.39065567542608903
2214	1	-1.8226566814020941
2214	2	-3.9071338552831776
2214	3	-2.01605500106324
2214	4	-1.8204577046172865
2214	5	-1.690589338283182
2214	6	-1.865859527400115
2214	7	-2.051041139196549
2214	8	-1.7570912980112752
2214	9	-1.912528789598781
2214	10	-1.812370813434761
2214	11	-1.5686159427183384
2214	12	-2.176612623755492
2214	13	-1.9214257605757403
2214	14	-3.179977698592918
2214	15	-3.323216262262285
2214	16	-2.617911429336637
2214	17	-2.971844365793821
2214	18	-2.244199538918039
2214	19	-4.4721831667768726
2214	20	-2.8534067530636698
2214	21	-2.8164291732852966
2214	22	-2.817403691799594
2214	23	-2.8956232152326713
2214	24	-3.01407016752815
2214	25	-2.8442541298083484
2215	1	-0.9920105885305865
2215	2	-2.546354906146906
2215	3	-1.388888541984489
2215	4	-1.0067218212348052
2215	5	-0.985366839845419
2215	6	-1.63674608976984
2215	7	-1.9234865717083096
2215	8	-1.711984298590135
2215	9	-1.4739931411700127
2215	10	-3.2180691016656766
2215	11	-2.201068007813138
2215	12	-2.391599746484708
2215	13	-1.7032406330775487
2215	14	-2.980237950750425
2215	15	-2.7315209083952254
2215	16	-2.3814744435642936
2215	17	-2.52448591133912
2215	18	-1.5358243256821993
2215	19	-2.080613005045855
2215	20	-2.3859043851974278
2215	21	-2.052656866109743
2215	22	-1.6851288436968304
2215	23	-2.169053049239068
2215	24	-2.023459790860521
2215	25	-1.8414241992970162
2216	1	-1.83350194865519
2216	2	-1.8168389705215553
2216	3	-2.323331471232983
2216	4	-2.883281414054405
2216	5	-2.0926700028576946
2216	6	-2.289391084246706
2216	7	-2.0556576845790033
2216	8	-2.593919171232212
2216	9	-2.9507788729119007
2216	10	-2.47234708635731
2216	11	-3.101684594809825
2216	12	-3.497888298446153
2216	13	-4.024434477145963
2216	14	-3.262184553941786
2216	15	-3.1172975359274737
2216	16	-2.4180896424459846
2216	17	-2.4641953684427804
2216	18	-2.565155586618495
2216	19	-2.938986580680197
2216	20	-2.693979972295125
2216	21	-3.1542014698941507
2216	22	-3.87430187255724
2216	23	-3.611225921692153
2216	24	-3.720258491376338
2216	25	-3.605364002028278
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95507.0
3	2.0
4	746.0
5	4581.0
6	5730.0
7	5047.0
8	4512.0
9	4344.0
10	4043.0
11	3917.0
12	3642.0
13	3600.0
14	3793.0
15	3774.0
16	3804.0
17	4007.0
18	4527.0
19	5435.0
20	6962.0
21	8908.0
22	11213.0
23	13857.0
24	16304.0
25	18473.0
26	20526.0
27	22663.0
28	26235.0
29	30387.0
30	37361.0
31	47233.0
32	59570.0
33	75947.0
34	99456.0
35	130614.0
36	178808.0
37	277128.0
38	749283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.581679440848056	19.177293344679207	12.6618310684415	25.579196146031236
2	14.355653702882462	20.529342663467784	41.93266975070702	23.18233388294274
3	18.229765658193024	25.24229181084314	30.1485440394055	26.37939849155833
4	11.8918324413283	17.27027878065917	39.22660978994148	31.611278988071057
5	12.583415072193871	38.2125029569127	36.656777645689786	12.547304325203646
6	30.61178480206106	38.016638891664435	18.827585271934762	12.543991034339744
7	27.09528665631319	31.696258437307474	23.391884217772535	17.816570688606802
8	24.260301844650833	37.63999582221936	21.244617090336472	16.855085242793326
9	26.261145252701045	15.217647823823036	20.702189207062062	37.81901771641385
10	14.47642805244783	29.091140980778203	35.514408189437226	20.918022777336745
11	33.5705000133061	22.428469642246533	25.831367963016056	18.169662381431305
12	22.96045793477442	24.802791795335292	32.728779091662275	19.507971178228015
13	28.73825475722188	22.459078376601955	26.065865247739072	22.736801618437088
14	20.361301791193178	22.6744869213271	28.204186983092384	28.760024304387343
15	22.310390454932346	31.89241712467893	23.634688246179188	22.162504174209534
16	21.567394782112682	28.34935281044662	28.65323916137008	21.43001324607062
17	21.186077270166397	29.056703821128334	28.443198263023056	21.314020645682213
18	21.027464800766356	27.805811404280302	30.538419861746195	20.62830393320715
19	23.049428863531826	27.203700988416585	27.837404367029748	21.90946578102184
20	23.172544277915474	27.919691169322604	28.46194248390168	20.44582206886024
21	22.519404232199	27.132482059527568	27.44722807084497	22.900885637428463
22	22.768170294557887	28.814081014842124	27.502044878364256	20.915703812235726
23	21.73106355799436	28.439511042910283	28.194319469104755	21.6351059299906
24	22.66249778442353	27.317593409457107	28.81544863101225	21.204460175107112
25	22.279336924914723	27.822369871288554	28.37471485190376	21.52357835189296
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	241.0
1	241.0
2	392.5
3	544.0
4	544.0
5	544.0
6	1080.0
7	1616.0
8	1616.0
9	1616.0
10	2531.5
11	3447.0
12	3447.0
13	3447.0
14	6144.5
15	8842.0
16	8842.0
17	8842.0
18	16951.5
19	25061.0
20	25061.0
21	25061.0
22	40426.5
23	55792.0
24	55792.0
25	55792.0
26	80684.5
27	105577.0
28	105577.0
29	105577.0
30	136644.5
31	167712.0
32	167712.0
33	167712.0
34	188535.5
35	209359.0
36	209359.0
37	209359.0
38	223441.0
39	237523.0
40	237523.0
41	237523.0
42	245162.5
43	252802.0
44	252802.0
45	252802.0
46	253829.0
47	254856.0
48	254856.0
49	254856.0
50	241735.5
51	228615.0
52	228615.0
53	228615.0
54	200288.5
55	171962.0
56	171962.0
57	171962.0
58	146601.5
59	121241.0
60	121241.0
61	121241.0
62	98338.5
63	75436.0
64	75436.0
65	75436.0
66	58204.5
67	40973.0
68	40973.0
69	40973.0
70	29895.0
71	18817.0
72	18817.0
73	18817.0
74	13135.0
75	7453.0
76	7453.0
77	7453.0
78	5141.5
79	2830.0
80	2830.0
81	2830.0
82	1849.5
83	869.0
84	869.0
85	869.0
86	570.0
87	271.0
88	271.0
89	271.0
90	167.5
91	64.0
92	64.0
93	64.0
94	43.0
95	22.0
96	22.0
97	22.0
98	18.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35523176161519
2	0.04156753796175485
3	0.03740074369747266
4	0.0367481132705369
5	0.04227037072922414
6	0.01706879578139692
7	0.01696839110032988
8	0.02264125558061768
9	0.01988012685127406
10	0.0199805315323411
11	0.018675270678469573
12	0.019528710467539418
13	0.019478508127005897
14	0.026557038142232266
15	0.027310073250235073
16	0.020331947916075743
17	0.021737613451014312
18	0.1137083013084236
19	0.023645302391288085
20	0.02143639940781319
21	0.024699551542492017
22	0.018976484721670694
23	0.021838018132081354
24	0.017972437911000286
25	0.02158700642941375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1991939.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.6620142955659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.55900831398885	62.28786879243923
2	10.945509804324573	16.125365993568266
3	2.4660754472414936	5.449682545459409
4	0.8515873509089394	2.5091855846630953
5	0.38327565452163426	1.41164283712575
6	0.2074871366606462	0.9170352256105532
7	0.11931952437953754	0.6152521557409931
8	0.08710213056319016	0.5132894709375969
9	0.06011431392947853	0.3985327306837244
>10	0.28373845844095774	3.6716709116544908
>50	0.019200685623716508	0.9942951938931662
>100	0.014162238806403042	2.113894874373236
>500	0.00239325842744326	1.2327360559960487
>1k	0.0010256821831899489	1.7595476278544315
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4174	0.20954456938691396	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3873	0.19443366488632433	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3569	0.17917215336413414	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3304	0.16586853312275124	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2967	0.1489503443629549	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2947	0.14794629755228447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.020234053352035E-5	0.0
8	0.0	0.0	0.0	5.020234053352035E-5	0.0
9	0.0	0.0	0.0	2.008093621340814E-4	0.0
10	0.0	5.020234053352035E-5	0.0	2.008093621340814E-4	0.0
11	0.0	5.020234053352035E-5	0.0	2.008093621340814E-4	0.0
12	0.0	5.020234053352035E-5	0.0	2.008093621340814E-4	0.0
13	0.0	5.020234053352035E-5	0.0	2.008093621340814E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGGTT	130	2.0008883E-10	12.415954	11
ACGAAAT	160	3.6379788E-12	11.878628	12
TACACGG	65	8.038429E-4	11.690005	5
CGAAATC	155	2.5465852E-11	11.648719	13
GTATTAC	115	6.798109E-8	11.598768	1
TAGGACC	745	0.0	11.219267	4
GGCGTAG	60	0.0057512694	11.115487	1
GAGCGTG	60	0.0059039923	11.076421	7
GGCGAGG	900	0.0	10.971208	19
TAGGACA	815	0.0	10.721813	4
GGTATCA	990	0.0	10.682417	1
TCCAACG	215	0.0	10.607624	18
ACCTTTT	740	0.0	10.529979	15
TTAGGAC	1350	0.0	10.48312	3
GTGCTAT	200	1.8189894E-12	10.480316	1
TTATACT	210	0.0	10.402714	4
AGGACGT	1880	0.0	10.357001	5
GACGTGA	995	0.0	10.305127	7
CCAACGT	140	9.775795E-8	10.172479	19
TGTAGGA	2175	0.0	10.131338	2
>>END_MODULE