FastQCFastQC Report
Thu 26 May 2016
SRR1512945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991939
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT38410.19282718998925166No Hit
GTATCAACGCAGAGTACTTTTTTTT36150.1814814610286761No Hit
CTGTAGGACGTGGAATATGGCAAGA35490.17816810655346374No Hit
CTTTAGGACGTGAAATATGGCGAGG35200.17671223867799163No Hit
GTCCTAAAGTGTGTATTTCTCATTT35080.17610981059158942No Hit
GTCCTACAGTGTGCATTTCTCATTT27950.14031554179118938No Hit
TATCAACGCAGAGTACTTTTTTTTT26500.13303620241382894No Hit
GGTATCAACGCAGAGTACTTTTTTT23130.11611801365403258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCAC307.6894806E-419.0125733
GGACCGA456.697652E-414.7968616
TAGGACC8300.012.9530434
GGTATCA11150.012.1934521
AGTACCG550.003034865312.1089595
TACACCG657.9253415E-411.7097645
GTACCGT657.93874E-411.7074066
CCGGTTT907.5340195E-611.60129112
AGGACCT15200.011.3293895
TCGGGCT600.00581174411.0998794
GGCGAGG9950.011.06323119
TGTAGGA24500.010.7091232
GTAGGAC23350.010.6665833
GGACCTG14450.010.6642726
GTCTAAC909.441562E-510.5638691
TCCAACG1355.7771103E-810.54450918
AGGACGT22050.010.4850185
TGGCGAG23000.010.39780318
ACTGTTC5600.010.35358
TAGGACT4150.010.3165834
Produced by FastQC (version 0.11.5)