##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512945_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1991939 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.430398220025815 31.0 31.0 34.0 27.0 34.0 2 30.5565612199972 31.0 31.0 34.0 27.0 34.0 3 30.662528320395353 31.0 31.0 34.0 27.0 34.0 4 34.14571128935173 37.0 35.0 37.0 30.0 37.0 5 33.800872918297195 37.0 35.0 37.0 28.0 37.0 6 33.66975143315132 37.0 35.0 37.0 29.0 37.0 7 33.53608217922336 37.0 35.0 37.0 28.0 37.0 8 33.41570550102187 37.0 35.0 37.0 27.0 37.0 9 34.85682392884521 39.0 35.0 39.0 27.0 39.0 10 34.733650980276 39.0 35.0 39.0 27.0 39.0 11 34.77977990289863 39.0 35.0 39.0 27.0 39.0 12 34.625410215875085 39.0 35.0 39.0 27.0 39.0 13 34.61408908606137 39.0 35.0 39.0 27.0 39.0 14 35.656690792238116 40.0 36.0 41.0 27.0 41.0 15 35.67406682634358 40.0 36.0 41.0 27.0 41.0 16 35.643745616708145 40.0 36.0 41.0 27.0 41.0 17 35.62784402534415 40.0 36.0 41.0 27.0 41.0 18 35.546621658594965 40.0 36.0 41.0 27.0 41.0 19 35.540499483166904 40.0 36.0 41.0 26.0 41.0 20 35.509743019239046 40.0 36.0 41.0 26.0 41.0 21 35.455256410964395 40.0 36.0 41.0 26.0 41.0 22 35.41039258732321 40.0 36.0 41.0 26.0 41.0 23 35.30709524739462 40.0 36.0 41.0 25.0 41.0 24 35.29392265526204 40.0 36.0 41.0 25.0 41.0 25 35.20132092398412 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38337373421313714 1101 2 0.4667213123205656 1101 3 0.43925955048829834 1101 4 0.3420836261294724 1101 5 0.4782599842131674 1101 6 0.5637039009420235 1101 7 0.4862147305833062 1101 8 0.4614361946510286 1101 9 0.1293990403304619 1101 10 0.552924977072422 1101 11 0.5415038372248304 1101 12 0.5842154973528153 1101 13 0.5867248386369894 1101 14 0.6664239873251958 1101 15 0.5999519271484814 1101 16 0.8095367639967463 1101 17 0.7648864483996007 1101 18 0.2242707948541991 1101 19 0.537722912291926 1101 20 0.36600092062559497 1101 21 0.7335653900095593 1101 22 0.5623335667176832 1101 23 0.44209668628140264 1101 24 0.4255920413634513 1101 25 0.47866438059169525 1102 1 0.6227162250377987 1102 2 0.5127727968802418 1102 3 0.5592929861582654 1102 4 0.3834226547958224 1102 5 0.615654656985221 1102 6 0.5130793495313597 1102 7 0.574372104794648 1102 8 0.5475334873175726 1102 9 0.7707134214740918 1102 10 0.6704058232479895 1102 11 0.7597034441034793 1102 12 0.7402741416417129 1102 13 0.8026829369633504 1102 14 0.9520443039640014 1102 15 0.8487224272940708 1102 16 0.9809370530409467 1102 17 0.7796495180029694 1102 18 0.9115859937468755 1102 19 0.9908574399754713 1102 20 0.9772105874105605 1102 21 0.9835558761542842 1102 22 0.9888925778431386 1102 23 0.9582390436465857 1102 24 0.9154949646393007 1102 25 0.9746010793812658 1103 1 0.4395186796815125 1103 2 0.27275487730490156 1103 3 0.38157211101523103 1103 4 0.3208971780410863 1103 5 0.4054615249583122 1103 6 0.4881596231007279 1103 7 0.4552202416765425 1103 8 0.5714418565833839 1103 9 0.5788062862922345 1103 10 0.6071510958505826 1103 11 0.6874614579890377 1103 12 0.7346814590833972 1103 13 0.7718386565938431 1103 14 0.8971102397599395 1103 15 0.637383858260911 1103 16 0.6944705587374855 1103 17 0.762847169017121 1103 18 0.9298635379620563 1103 19 0.9643448154444627 1103 20 0.9125434255606564 1103 21 0.5953141182608519 1103 22 0.8842665591691699 1103 23 1.0328201894216988 1103 24 0.8968965374624034 1103 25 0.9949013379260805 1104 1 0.41916589457046527 1104 2 0.4525087420487175 1104 3 0.43939156804663426 1104 4 0.4679862411772717 1104 5 0.5212990284060481 1104 6 0.48762427235141814 1104 7 0.6079543919582662 1104 8 0.4544984519312436 1104 9 0.5893977201548779 1104 10 0.5618569550254904 1104 11 0.5226301670266906 1104 12 0.6104806106128748 1104 13 0.7538684187331413 1104 14 0.8466401660769733 1104 15 0.8211541670463944 1104 16 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2216 14 -1.959320218999764 2216 15 -2.1411346610121313 2216 16 -1.90357585709409 2216 17 -2.0530195525184425 2216 18 -1.8222176775199372 2216 19 -2.0071611118230024 2216 20 -2.337362149676629 2216 21 -2.254177495557151 2216 22 -2.6320320512487285 2216 23 -1.5724222693298628 2216 24 -1.820728094056932 2216 25 -1.6445705556825772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51614.0 3 1.0 4 830.0 5 7349.0 6 8489.0 7 6647.0 8 5306.0 9 4716.0 10 4055.0 11 3654.0 12 3340.0 13 3301.0 14 3092.0 15 3268.0 16 3198.0 17 3338.0 18 3829.0 19 4461.0 20 5785.0 21 7455.0 22 9546.0 23 11466.0 24 13803.0 25 16062.0 26 18297.0 27 19986.0 28 23053.0 29 27621.0 30 34935.0 31 44526.0 32 56656.0 33 73062.0 34 95363.0 35 124229.0 36 170807.0 37 265934.0 38 852865.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.07168217664545 19.885814134469832 13.66497541497127 27.37752827391344 2 13.339685612389154 20.969147603135845 40.75833761727284 24.932829167202158 3 16.637758485576114 25.456803647099633 30.520010903948364 27.385426963375885 4 11.17563075724013 17.887939368160552 38.54322082542183 32.39320904917749 5 12.2470398913174 38.58106706096264 36.57165480211598 12.600238245603986 6 30.853821759733762 38.38535323695031 18.346888452124006 12.413936551191926 7 26.915535725227187 31.88822940323859 23.305194981301923 17.891039890232303 8 24.19691134045895 37.93716570420292 20.93270725460189 16.93321570073624 9 25.926223587596304 15.025672760954498 20.59572257947204 38.45238107197716 10 14.229966781983963 29.500101277503877 35.36605409274205 20.90387784777011 11 33.52565344825682 22.47198288197014 25.726999153537122 18.27536451623592 12 22.824969786191172 25.04183740125595 32.52617567741945 19.607017135133425 13 28.629911266102287 22.393937537842692 26.10400251431119 22.87214868174383 14 20.150059294709266 22.695213144647962 28.110060218521838 29.044667342120935 15 22.16700589729094 32.02521184760027 23.528112294541216 22.279669960567578 16 21.419081161367686 28.425911140971138 28.51473803347604 21.640269664185137 17 21.070966737558912 29.184521552665743 28.375948194256473 21.368563515518872 18 20.93551679491215 27.831905789647283 30.55299934785326 20.679578067587304 19 23.00298717272887 27.247132063157366 27.887391118809145 21.862489645304617 20 23.150783486117852 27.85903023541987 28.39241631828047 20.597769960181804 21 22.248305284279997 27.155513229149463 27.57751664594134 23.0186648406292 22 22.64348912209678 28.62111481941114 27.674444056210866 21.06095200228121 23 21.496491609431814 28.31331682345694 28.30222210619904 21.887969460912206 24 22.625151987662267 27.328862645323298 28.850446148424297 21.195539218590138 25 22.183955076825757 27.80366946223225 28.37565171988784 21.636723741054148 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 149.0 1 149.0 2 317.5 3 486.0 4 486.0 5 486.0 6 1095.5 7 1705.0 8 1705.0 9 1705.0 10 2608.5 11 3512.0 12 3512.0 13 3512.0 14 6266.0 15 9020.0 16 9020.0 17 9020.0 18 17099.5 19 25179.0 20 25179.0 21 25179.0 22 40852.0 23 56525.0 24 56525.0 25 56525.0 26 81768.5 27 107012.0 28 107012.0 29 107012.0 30 137308.0 31 167604.0 32 167604.0 33 167604.0 34 188063.0 35 208522.0 36 208522.0 37 208522.0 38 222794.5 39 237067.0 40 237067.0 41 237067.0 42 245170.5 43 253274.0 44 253274.0 45 253274.0 46 254475.0 47 255676.0 48 255676.0 49 255676.0 50 243017.5 51 230359.0 52 230359.0 53 230359.0 54 201280.0 55 172201.0 56 172201.0 57 172201.0 58 146243.0 59 120285.0 60 120285.0 61 120285.0 62 97522.5 63 74760.0 64 74760.0 65 74760.0 66 57386.5 67 40013.0 68 40013.0 69 40013.0 70 29038.0 71 18063.0 72 18063.0 73 18063.0 74 12501.5 75 6940.0 76 6940.0 77 6940.0 78 4725.5 79 2511.0 80 2511.0 81 2511.0 82 1655.0 83 799.0 84 799.0 85 799.0 86 505.0 87 211.0 88 211.0 89 211.0 90 134.0 91 57.0 92 57.0 93 57.0 94 31.0 95 5.0 96 5.0 97 5.0 98 4.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07530351080028053 2 1.5060702160056105E-4 3 0.0 4 0.03222990262252007 5 0.07811484187015767 6 0.07766302080535599 7 0.04141693094015429 8 0.07836585357282527 9 0.05587520501380815 10 0.11827671429697395 11 0.006074483204555963 12 0.015060702160056106 13 0.00707853001522637 14 0.009689051722969428 15 0.008885814274433102 16 0.02133599472674615 17 0.03283233070892232 18 0.00281133106987714 19 0.004468008307483312 20 0.006827518312558768 21 6.024280864022442E-4 22 5.522257458687238E-4 23 0.0 24 5.020234053352035E-5 25 0.004719020010150913 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1991939.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.84273663598358 #Duplication Level Percentage of deduplicated Percentage of total 1 84.06127811344464 61.232535429018185 2 11.222723207798936 16.349877419284773 3 2.6007061903840984 5.683276682811632 4 0.870106969459469 2.5352389128587967 5 0.4066741222467538 1.4811627991745042 6 0.21820887752234475 0.9536959078196254 7 0.12994977021293636 0.6626127821269254 8 0.08476576973644277 0.4939656512526503 9 0.06680820958100353 0.4379843534067572 >10 0.30134095892178264 3.859393114435558 >50 0.018797151463797693 0.9692181954523044 >100 0.015328292526080575 2.240466377666034 >500 0.0021392368282236287 1.1499091087101674 >1k 0.001173129873541979 1.9506632659820975 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3841 0.19282718998925166 No Hit GTATCAACGCAGAGTACTTTTTTTT 3615 0.1814814610286761 No Hit CTGTAGGACGTGGAATATGGCAAGA 3549 0.17816810655346374 No Hit CTTTAGGACGTGAAATATGGCGAGG 3520 0.17671223867799163 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3508 0.17610981059158942 No Hit GTCCTACAGTGTGCATTTCTCATTT 2795 0.14031554179118938 No Hit TATCAACGCAGAGTACTTTTTTTTT 2650 0.13303620241382894 No Hit GGTATCAACGCAGAGTACTTTTTTT 2313 0.11611801365403258 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.020234053352035E-5 0.0 2 0.0 0.0 0.0 5.020234053352035E-5 0.0 3 0.0 0.0 0.0 5.020234053352035E-5 0.0 4 0.0 0.0 0.0 5.020234053352035E-5 0.0 5 0.0 0.0 0.0 5.020234053352035E-5 0.0 6 0.0 0.0 0.0 5.020234053352035E-5 0.0 7 0.0 0.0 0.0 1.004046810670407E-4 0.0 8 0.0 0.0 0.0 1.004046810670407E-4 0.0 9 0.0 5.020234053352035E-5 0.0 1.004046810670407E-4 0.0 10 0.0 5.020234053352035E-5 0.0 1.004046810670407E-4 0.0 11 0.0 5.020234053352035E-5 0.0 1.004046810670407E-4 0.0 12 0.0 5.020234053352035E-5 0.0 1.004046810670407E-4 0.0 13 0.0 5.020234053352035E-5 0.0 1.004046810670407E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGCAC 30 7.6894806E-4 19.012573 3 GGACCGA 45 6.697652E-4 14.796861 6 TAGGACC 830 0.0 12.953043 4 GGTATCA 1115 0.0 12.193452 1 AGTACCG 55 0.0030348653 12.108959 5 TACACCG 65 7.9253415E-4 11.709764 5 GTACCGT 65 7.93874E-4 11.707406 6 CCGGTTT 90 7.5340195E-6 11.601291 12 AGGACCT 1520 0.0 11.329389 5 TCGGGCT 60 0.005811744 11.099879 4 GGCGAGG 995 0.0 11.063231 19 TGTAGGA 2450 0.0 10.709123 2 GTAGGAC 2335 0.0 10.666583 3 GGACCTG 1445 0.0 10.664272 6 GTCTAAC 90 9.441562E-5 10.563869 1 TCCAACG 135 5.7771103E-8 10.544509 18 AGGACGT 2205 0.0 10.485018 5 TGGCGAG 2300 0.0 10.397803 18 ACTGTTC 560 0.0 10.3535 8 TAGGACT 415 0.0 10.316583 4 >>END_MODULE