FastQCFastQC Report
Thu 26 May 2016
SRR1512944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912183
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT35730.18685450085059851No Hit
CTGTAGGACGTGGAATATGGCAAGA32940.17226384713178602No Hit
GTCCTAAAGTGTGTATTTCTCATTT31160.16295511465168344No Hit
GTATCAACGCAGAGTACTTTTTTTT30900.1615954121545898No Hit
CTTTAGGACGTGAAATATGGCGAGG27880.14580194468834834No Hit
GTCCTACAGTGTGCATTTCTCATTT20830.10893308851715552No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCCG400.005296888314.2430169
TAGGACC6650.013.1388224
CCAACGA1001.453318E-712.34330119
GGCGAGG7550.011.6956419
AGGACCG658.047365E-411.6884125
GGTATCA9800.011.372571
CCATGCG600.00589797511.0779029
GTGCCGT600.00589797511.07790211
TCCAACG1605.4205884E-1010.69065118
GACGTGG8800.010.4653647
GCCGGTT1002.4121784E-510.44487811
GGTCTAC2100.010.4329551
GTCCTAA9550.010.3735831
TGTAGGA19900.010.2609362
GTAGGAC19600.010.2239013
AGGACCT11600.010.1517895
GGACCTG11050.010.1387646
GGCGTGC750.00265941510.1281028
CTGTAGG19650.010.0832281
GGACGTG19400.010.0816316
Produced by FastQC (version 0.11.5)