##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512944_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1912183
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.960115742060253	31.0	30.0	34.0	23.0	34.0
2	28.918506753799193	31.0	30.0	34.0	20.0	34.0
3	29.183450537945376	31.0	30.0	34.0	23.0	34.0
4	32.59871780054524	35.0	33.0	37.0	25.0	37.0
5	32.77219282882444	35.0	35.0	37.0	26.0	37.0
6	32.74935453353576	36.0	35.0	37.0	25.0	37.0
7	32.61026847325805	36.0	33.0	37.0	25.0	37.0
8	32.61705914130604	37.0	33.0	37.0	25.0	37.0
9	33.942752341172366	39.0	34.0	39.0	23.0	39.0
10	33.8141302375348	38.0	34.0	39.0	23.0	39.0
11	33.88897819926231	38.0	34.0	39.0	23.0	39.0
12	33.78651467981882	38.0	34.0	39.0	22.0	39.0
13	33.78111143128037	38.0	34.0	39.0	22.0	39.0
14	34.76005068552539	39.0	34.0	41.0	21.0	41.0
15	34.70607572601576	39.0	34.0	41.0	19.0	41.0
16	34.59772417179737	39.0	34.0	41.0	18.0	41.0
17	34.53197418866291	39.0	34.0	41.0	18.0	41.0
18	34.235347244484444	39.0	34.0	41.0	17.0	41.0
19	34.47421664139886	39.0	34.0	41.0	18.0	41.0
20	34.56179560219916	39.0	34.0	41.0	18.0	41.0
21	34.494159293331236	39.0	34.0	41.0	18.0	41.0
22	34.39758067088767	39.0	34.0	41.0	18.0	41.0
23	34.1265977158044	39.0	34.0	41.0	10.0	41.0
24	34.14769820670929	39.0	34.0	41.0	8.0	41.0
25	34.052770053912205	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.708994706891886
1101	2	0.8625621750741317
1101	3	0.9776618038668907
1101	4	0.9395242806252213
1101	5	0.8346537353187031
1101	6	0.9822984619247421
1101	7	1.0928076262422337
1101	8	0.8053427302509704
1101	9	0.6488747700059534
1101	10	0.7747317082709841
1101	11	0.8014463333849591
1101	12	1.0663005100560596
1101	13	0.8810296338757198
1101	14	1.021079963055925
1101	15	0.6948306542270757
1101	16	1.25757125870215
1101	17	1.1508700189801218
1101	18	1.4717959215128502
1101	19	0.8737278045585128
1101	20	0.9854490840097085
1101	21	1.0372183251192766
1101	22	1.1115298090496353
1101	23	1.341920390221759
1101	24	0.9410148262821139
1101	25	1.2020478899261136
1102	1	0.4447812716573871
1102	2	0.4786557525099546
1102	3	0.5622919054768722
1102	4	0.6105739972778252
1102	5	0.631428651008676
1102	6	0.5428741438768157
1102	7	0.7656382372182691
1102	8	0.7595073557029224
1102	9	0.6498097104071476
1102	10	0.8591912912878001
1102	11	0.8052980269350982
1102	12	0.7652038099402603
1102	13	0.7552968639991846
1102	14	0.8244812266173369
1102	15	0.902166587736005
1102	16	0.9618953959118457
1102	17	0.7385672422525573
1102	18	1.0962353591089808
1102	19	1.087679137340686
1102	20	0.9178513904833352
1102	21	0.7760656054525441
1102	22	1.011134308411009
1102	23	1.3009343128785318
1102	24	1.1084417216766482
1102	25	1.0832501252105544
1103	1	0.5462282958451041
1103	2	0.6640148276245199
1103	3	0.5831214366714654
1103	4	0.7078248188794376
1103	5	0.2662186393565804
1103	6	0.7334988509328753
1103	7	0.43499889372063905
1103	8	0.5075065061371902
1103	9	0.9074334986098691
1103	10	0.7934959946485236
1103	11	0.8024153453506102
1103	12	0.9858970755015548
1103	13	0.86571618084146
1103	14	1.1029868513674188
1103	15	1.1241348811821652
1103	16	1.1684865949362049
1103	17	1.001334843003761
1103	18	1.3217948339179557
1103	19	1.324266210808652
1103	20	1.2960272424379014
1103	21	1.0229543426835832
1103	22	1.2673229826491905
1103	23	1.465153194703305
1103	24	1.2905288818673384
1103	25	1.1401295923933645
1104	1	0.3445090223503513
1104	2	0.41117537463249576
1104	3	0.4319960528527602
1104	4	0.575472954481981
1104	5	0.3728306283558638
1104	6	0.38576051159805047
1104	7	0.4571168092254396
1104	8	0.4849644722297981
1104	9	0.6381524452011362
1104	10	0.7030988463463146
1104	11	0.6647826717645273
1104	12	0.5710105984238893
1104	13	0.584521849902039
1104	14	0.7603951578699579
1104	15	0.8366144835089244
1104	16	0.6727417472707486
1104	17	0.6870433933434086
1104	18	1.024063039411601
1104	19	0.8045529846903463
1104	20	0.8115744205128834
1104	21	0.8301618322203055
1104	22	0.7895942116057668
1104	23	1.0943384210090557
1104	24	0.9932268381460787
1104	25	0.7036541931757228
1105	1	0.6021251265935881
1105	2	0.7717376866939709
1105	3	0.7679261118793796
1105	4	0.8794125914125956
1105	5	0.5988365310176249
1105	6	0.4814673831873151
1105	7	0.7423293050490329
1105	8	0.7527173400324472
1105	9	0.713130059634814
1105	10	0.9838787772963045
1105	11	1.0031341585670646
1105	12	0.9448516720401017
1105	13	0.9396557178160663
1105	14	1.1081743092231164
1105	15	1.166003390959645
1105	16	1.2667707037246956
1105	17	1.0901752565798475
1105	18	1.209118502158013
1105	19	1.163907478509259
1105	20	1.1500533157127961
1105	21	1.0710660531803242
1105	22	0.9391669925390573
1105	23	1.1871673551888051
1105	24	1.204565294544338
1105	25	1.2628751531935407
1106	1	0.8658182153587362
1106	2	0.9208949090981662
1106	3	1.0058302498857117
1106	4	1.0507026885847708
1106	5	1.0009276981756159
1106	6	0.9371730279083508
1106	7	0.8229513161856161
1106	8	1.0053199167329652
1106	9	1.1449125686423471
1106	10	1.1612144034563698
1106	11	1.1030994386194735
1106	12	1.208668791813004
1106	13	1.1894114771784388
1106	14	1.361642045875584
1106	15	1.4088854741564987
1106	16	1.398127183689759
1106	17	1.4519460830892896
1106	18	1.6389609522071424
1106	19	1.1538491752010316
1106	20	1.352035846556774
1106	21	1.4421481582136266
1106	22	1.3569097592166415
1106	23	1.5611021734003998
1106	24	1.4785069912361166
1106	25	1.5599441563598262
1107	1	0.4871251265935861
1107	2	0.5658026217589054
1107	3	0.6692291421824095
1107	4	0.8398628078628079
1107	5	0.8039317691128645
1107	6	0.6731210628409912
1107	7	0.8985111232308469
1107	8	0.8069727512878586
1107	9	1.0681690206737784
1107	10	0.6730692534867799
1107	11	0.7060042884371924
1107	12	0.8434837066721386
1107	13	0.8639717351320826
1107	14	1.090096387145195
1107	15	1.188678715634964
1107	16	1.0601516561056457
1107	17	1.1422575076620944
1107	18	1.3919886320281378
1107	19	1.228232153833929
1107	20	1.021360675020155
1107	21	1.1082695163837855
1107	22	1.038643183015246
1107	23	1.190180342201792
1107	24	1.1889289309079771
1107	25	1.160364330682718
1108	1	0.5865399510409226
1108	2	0.60251922115431
1108	3	0.5594614391442256
1108	4	0.6317134149930013
1108	5	0.6850389584125125
1108	6	0.7929689003091198
1108	7	0.8276309955561842
1108	8	0.8166319477480997
1108	9	1.1166229074891731
1108	10	0.9220846723981708
1108	11	0.8872199843147897
1108	12	0.9584073720834496
1108	13	0.9103314872135471
1108	14	1.179632046544313
1108	15	1.211630178996053
1108	16	1.1172527149947413
1108	17	1.1454231976288298
1108	18	1.1814943149018333
1108	19	1.1696500012660849
1108	20	1.2152175983309164
1108	21	1.1168584242249722
1108	22	1.0366043570817496
1108	23	1.1411712563591578
1108	24	1.2482089876522764
1108	25	1.4824958727427386
1109	1	0.14045956040012442
1109	2	0.12233557378542059
1109	3	0.3749968723063617
1109	4	0.4945636863834295
1109	5	0.3490433099325614
1109	6	0.21424431726897097
1109	7	0.22276080059211267
1109	8	0.39027550004125544
1109	9	0.538146896874828
1109	10	0.7024966930000573
1109	11	0.35303247484306155
1109	12	0.5440255468062531
1109	13	0.5817608130069942
1109	14	0.33984845910756434
1109	15	0.662393948899556
1109	16	0.5915799917185325
1109	17	0.4796312926753643
1109	18	-0.8060558127974176
1109	19	0.44471170432635887
1109	20	0.6164001848665279
1109	21	0.6704512188015386
1109	22	0.7114854694841455
1109	23	0.29513665198643224
1109	24	0.6997095665813191
1109	25	0.7520371428490904
1110	1	0.36763911488246137
1110	2	0.32732194173626183
1110	3	0.4968256998212546
1110	4	0.2762969982657708
1110	5	0.5386190077038151
1110	6	0.4462108227882666
1110	7	0.45640282489288353
1110	8	0.3260252992603796
1110	9	0.5890103459067717
1110	10	0.6824892739347774
1110	11	0.5416026035898369
1110	12	0.08218175228299884
1110	13	0.2728539394599565
1110	14	0.4053590847598727
1110	15	0.5242018294762332
1110	16	0.5523326208793264
1110	17	0.29909110997390087
1110	18	0.7344672334527402
1110	19	0.5243290790297479
1110	20	0.5169546386362356
1110	21	0.5125080397342145
1110	22	0.569740403946561
1110	23	0.5447255855076136
1110	24	0.8349986061904033
1110	25	0.7212995730590777
1111	1	0.24253423306397437
1111	2	0.472042034520058
1111	3	0.382116229419033
1111	4	0.291374476611729
1111	5	0.30747752154598373
1111	6	0.4004969392754134
1111	7	0.300228312238346
1111	8	0.14762308007581026
1111	9	0.45665077171971546
1111	10	0.47874001203327055
1111	11	0.25039810926887895
1111	12	0.32981766542136626
1111	13	0.30575648239440056
1111	14	0.32652727053773134
1111	15	0.37219407908015256
1111	16	0.2785883460846179
1111	17	0.21511682910067265
1111	18	0.5036453765161042
1111	19	0.26156033711932025
1111	20	0.12631783699546162
1111	21	0.38665854433632063
1111	22	0.26860234572860264
1111	23	0.5792971052743923
1111	24	0.71151770810134
1111	25	0.37564874671174664
1112	1	-0.32288815802946047
1112	2	0.03224881256577561
1112	3	0.13016102829695697
1112	4	0.09522151425107239
1112	5	0.05515347998253617
1112	6	0.1106820405547424
1112	7	-0.1954677723338989
1112	8	0.13926322733455976
1112	9	0.3510523445899807
1112	10	0.26025196449878507
1112	11	0.3784506647112025
1112	12	0.389885105008112
1112	13	0.14457446020507803
1112	14	0.2883201079610771
1112	15	0.27040491869129113
1112	16	0.364233119311983
1112	17	0.5289146565577028
1112	18	0.4007767552300763
1112	19	0.25922597734685127
1112	20	0.2404713385180628
1112	21	0.2616192470251164
1112	22	0.16711141853263456
1112	23	0.47663962353819045
1112	24	-0.03650810299800611
1112	25	0.28926561040598386
1113	1	-0.4207661200907644
1113	2	-0.17058857325297794
1113	3	-0.38293507291284
1113	4	-0.06805513626203208
1113	5	-0.3643137784430266
1113	6	-0.15896409338209594
1113	7	-0.3536229495928822
1113	8	-0.057951979154111655
1113	9	-0.13816171755351547
1113	10	-0.3857693217311038
1113	11	-0.26458202180428714
1113	12	-0.066634623273778
1113	13	-0.28866700543769497
1113	14	-0.24956309130738674
1113	15	-0.5607976700483164
1113	16	-0.4132284121020078
1113	17	-0.58426683006914
1113	18	-0.6049239381602902
1113	19	-0.5009386753368972
1113	20	-0.5562711758875594
1113	21	-0.542356581656108
1113	22	-0.30364556272177623
1113	23	-0.817787551972998
1113	24	-0.18490154895698652
1113	25	-0.20169602275694132
1114	1	-1.5901998649518987
1114	2	-1.2500029287947
1114	3	-1.060851252566568
1114	4	-1.3230477850951274
1114	5	-1.2429787096542242
1114	6	-1.7946512054723627
1114	7	-2.006574651663854
1114	8	-1.3960592353254597
1114	9	-1.5390823177747208
1114	10	-1.8045697628908215
1114	11	-2.473434661182683
1114	12	-1.7659059652048512
1114	13	-1.863782789680272
1114	14	-2.01612295151412
1114	15	-1.9456939374137079
1114	16	-2.7578166032305518
1114	17	-4.402489493730155
1114	18	-3.5703130162728307
1114	19	-2.602630228626353
1114	20	-1.8664158534902242
1114	21	-2.8821264843013594
1114	22	-2.962319423873975
1114	23	-2.6612306833637795
1114	24	-2.3912629097167333
1114	25	-2.2606853247683567
1115	1	-1.3469530856969172
1115	2	-0.9028516987808892
1115	3	-0.882396048269598
1115	4	-0.9329887121255815
1115	5	-0.8600247352765997
1115	6	-0.9608855032186447
1115	7	-2.0101734882470588
1115	8	-1.4722063098744407
1115	9	-1.2434984319852944
1115	10	-1.0292850960742683
1115	11	-1.9547317632206465
1115	12	-1.4308596873639914
1115	13	-1.2486189562993957
1115	14	-1.364431836028281
1115	15	-2.6488457710515334
1115	16	-1.4790542497203703
1115	17	-1.4506776298261101
1115	18	-1.522099374936964
1115	19	-1.5723020186974495
1115	20	-1.2902977084957712
1115	21	-2.1428994961679066
1115	22	-2.041195204853871
1115	23	-1.476232086151974
1115	24	-1.3866106831092893
1115	25	-1.8587216810708895
1116	1	-1.6602293707609093
1116	2	-2.4550647824418306
1116	3	-1.9923349110483102
1116	4	-1.725881941215274
1116	5	-2.253211088029989
1116	6	-1.8396895833029845
1116	7	-1.7304731640867743
1116	8	-2.3186565576747817
1116	9	-2.498778229606806
1116	10	-3.415555085137555
1116	11	-1.9736453828791447
1116	12	-1.9761130545912877
1116	13	-2.4600462222192085
1116	14	-2.243248971200167
1116	15	-1.9074692721796893
1116	16	-1.8069118359578482
1116	17	-2.2246977592931714
1116	18	-2.0329432262370517
1116	19	-2.3415810576459464
1116	20	-2.702761499102021
1116	21	-3.097306080858477
1116	22	-2.3321240127519474
1116	23	-2.2907920804372957
1116	24	-3.0123377036919905
1116	25	-2.624929079610691
1201	1	0.37828958011759184
1201	2	0.42734987600278984
1201	3	0.5313077938677999
1201	4	0.6331674433022769
1201	5	0.6369975797067653
1201	6	0.6632664979302518
1201	7	0.5200722400166811
1201	8	0.18157025116625647
1201	9	0.45209090403385943
1201	10	0.6759560670477569
1201	11	0.28737862571041006
1201	12	-0.37253239333272603
1201	13	0.1639522789328467
1201	14	0.6283898352700987
1201	15	0.3135182019913074
1201	16	0.7562640642967082
1201	17	0.8803819300561813
1201	18	1.076384760244494
1201	19	0.800294267749976
1201	20	0.8423903943644788
1201	21	0.7236922023120869
1201	22	0.745417588385159
1201	23	1.0501115158970151
1201	24	0.8719728532777395
1201	25	0.7753930285765875
1202	1	0.8104763150770147
1202	2	0.74829711086538
1202	3	0.5577437661590352
1202	4	0.8727653422216548
1202	5	0.8130187651432834
1202	6	0.8450018101485881
1202	7	0.8154397837902394
1202	8	0.8326830804632053
1202	9	1.2168220582956764
1202	10	1.2876777952630505
1202	11	0.9457943527418209
1202	12	1.0953236059760485
1202	13	1.1008349388851428
1202	14	1.246548263022916
1202	15	0.9495323497812009
1202	16	1.093779519705052
1202	17	1.2814107213717207
1202	18	1.4410605845148936
1202	19	1.1857581648165905
1202	20	1.1715332844662854
1202	21	1.1217455532361171
1202	22	1.3038555317645901
1202	23	1.3759912587709948
1202	24	1.4273895329105954
1202	25	1.2970839468549613
1203	1	0.7103123104047171
1203	2	0.8584779902352864
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2211	16	0.3541644010171936
2211	17	0.47015883403213365
2211	18	0.45119972719130175
2211	19	0.31019775813641814
2211	20	0.23699783413267994
2211	21	0.12224093112084944
2211	22	0.011626823875054981
2211	23	0.5812299384111768
2211	24	0.3292630308958735
2211	25	0.35565588998892395
2212	1	-0.25875991586232416
2212	2	-0.3581911180088362
2212	3	-0.2748938315127418
2212	4	-0.3701972496498911
2212	5	-0.394864535823217
2212	6	-0.45606451317672025
2212	7	-0.5306602442651425
2212	8	-0.49096892167014516
2212	9	-0.5068803910510127
2212	10	-0.4984293002129476
2212	11	-0.6258416742219666
2212	12	-0.39940545837574604
2212	13	-0.4614000191750094
2212	14	-0.5482600474065364
2212	15	-0.5970188167477417
2212	16	-0.49050384450086426
2212	17	-0.4624715973282747
2212	18	-2.962244659174452
2212	19	-1.34439820405116
2212	20	-0.7068014632987456
2212	21	-0.706594081807701
2212	22	-0.5371966042386518
2212	23	-0.5591285298536448
2212	24	-1.0007985199559855
2212	25	-0.5614041849296925
2213	1	-0.29822027917435534
2213	2	-0.1466264984166905
2213	3	-0.5181664434526141
2213	4	-0.7237034380978038
2213	5	-0.6169267151258992
2213	6	-0.5497251050755949
2213	7	-0.4266626466612351
2213	8	-0.7973410100681555
2213	9	-0.6511194373470772
2213	10	-0.6806195459766613
2213	11	-0.6590938763088587
2213	12	-0.6540423588116724
2213	13	-0.46910551625502706
2213	14	-0.570337427866086
2213	15	-0.6707551603482074
2213	16	-1.24241373624848
2213	17	-0.756108445633437
2213	18	-0.5031397138044298
2213	19	-0.5623004356423209
2213	20	-1.3527354166815684
2213	21	-0.9442867301865476
2213	22	-0.7925607069914591
2213	23	-1.2978889828393605
2213	24	-0.8030349737319824
2213	25	-1.0735467113269124
2214	1	-1.88227992356207
2214	2	-3.767065841496965
2214	3	-2.129628598140222
2214	4	-1.840919243016092
2214	5	-1.7066182135920798
2214	6	-1.8706165554728962
2214	7	-1.914629186822296
2214	8	-1.713304912936536
2214	9	-2.006515737667158
2214	10	-2.0771382871565933
2214	11	-1.951096903349228
2214	12	-2.3283009856331347
2214	13	-2.2100605280042984
2214	14	-3.341864085795912
2214	15	-3.623483637750148
2214	16	-2.805336064406287
2214	17	-3.2343061224124305
2214	18	-2.677175859654511
2214	19	-4.909657377941798
2214	20	-3.13814845991271
2214	21	-3.088783249252515
2214	22	-3.0254609332075155
2214	23	-3.1408572037873235
2214	24	-3.3784966217475976
2214	25	-3.366643467814182
2215	1	-1.4484801456922263
2215	2	-3.02678919502646
2215	3	-1.8548043771945046
2215	4	-1.3372707245776958
2215	5	-1.2659359282262166
2215	6	-1.9639380972150455
2215	7	-2.3203037161094855
2215	8	-2.294900072385172
2215	9	-2.023688532109894
2215	10	-3.5745572268660233
2215	11	-2.4913042736216298
2215	12	-2.862246142736172
2215	13	-2.1075239561346812
2215	14	-3.5505468146062285
2215	15	-3.342977184829465
2215	16	-3.0948394246138413
2215	17	-3.21229335250791
2215	18	-2.0578606861874604
2215	19	-2.797794914831016
2215	20	-2.9368540724245626
2215	21	-2.6103709915647357
2215	22	-2.1167046689246973
2215	23	-2.6518361134202806
2215	24	-2.5692175705267637
2215	25	-2.538847011218529
2216	1	-2.5586428413013422
2216	2	-2.398649398311502
2216	3	-2.8607607420668693
2216	4	-3.6935824225725717
2216	5	-2.8101819537203028
2216	6	-2.6811282631704607
2216	7	-2.6742037120979987
2216	8	-3.278738454689673
2216	9	-3.532435792244062
2216	10	-3.0087700126830192
2216	11	-3.6110146922173207
2216	12	-4.16807584832959
2216	13	-4.663729786257875
2216	14	-3.971143822845473
2216	15	-3.812054617066625
2216	16	-3.0303327864856904
2216	17	-3.2335115973664017
2216	18	-3.186075587590256
2216	19	-3.5800823062408362
2216	20	-3.4444345840804687
2216	21	-3.7253492453725165
2216	22	-4.257417952128339
2216	23	-4.376119401469346
2216	24	-4.202888919246245
2216	25	-4.381666376661741
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96943.0
3	3.0
4	792.0
5	4705.0
6	5596.0
7	4926.0
8	4632.0
9	4292.0
10	3937.0
11	3915.0
12	3738.0
13	3529.0
14	3611.0
15	3581.0
16	3867.0
17	3948.0
18	4472.0
19	5442.0
20	6946.0
21	8881.0
22	11189.0
23	13184.0
24	15287.0
25	17464.0
26	19578.0
27	21617.0
28	24506.0
29	29177.0
30	35621.0
31	45089.0
32	56653.0
33	72447.0
34	94316.0
35	124030.0
36	170454.0
37	269687.0
38	714128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77369059996924	19.020816352023665	12.605594660315198	25.599898387691894
2	14.39705306862152	20.4388169583533	41.85649143146533	23.307638541559854
3	18.34109429873779	25.24534538257871	30.051546202986255	26.362014115697242
4	11.939638711162496	17.168962432447984	39.26931346750441	31.62208538888511
5	12.637523327105797	38.19341080870962	36.567537039546345	12.601528824638237
6	30.696445850877424	38.00088919109763	18.704291654680024	12.598373303344928
7	26.99672514950234	31.87939595458414	23.229051757911083	17.89482713800244
8	24.31573292490468	37.42918015584943	21.298706869269875	16.956380049976016
9	26.27988454702282	15.23579669981912	20.508491066412663	37.9758276867454
10	14.48715675366922	29.046026667252505	35.46108528007624	21.005731299002033
11	33.66390959785293	22.496865540719728	25.657948763817384	18.181276097609967
12	22.9173586443288	24.813904867216134	32.60476694366696	19.663969544788106
13	28.57579244690867	22.37446385605189	26.152787948530182	22.89695574850926
14	20.53041173396553	22.5004930303555	28.246585274373352	28.72250996130562
15	22.414002349836945	31.53411082316483	23.657919818876326	22.393967008121898
16	21.658328834879544	28.403289520485746	28.329797339426122	21.60858430520858
17	21.287768583715053	28.915527783313742	28.307181778284818	21.489521854686384
18	21.115730557059116	27.909544245299912	30.208681231357463	20.76604396628351
19	23.04734803407447	27.26548952631042	27.860301983318468	21.826860456296636
20	23.1679351379626	27.766653589643	28.425997123054792	20.63941414933961
21	22.591984633395374	27.172620075138177	27.497727127685728	22.737668163780718
22	22.810854296169676	28.487872136985327	27.570828686742725	21.13044488010227
23	22.001203086177586	28.196573819798616	28.007950830391003	21.7942722636328
24	22.70395833899986	27.268528495479682	28.695290122668965	21.332223042851496
25	22.397948268367585	27.685247712698985	28.27789811539264	21.63890590354079
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	240.0
1	240.0
2	370.0
3	500.0
4	500.0
5	500.0
6	1047.0
7	1594.0
8	1594.0
9	1594.0
10	2502.5
11	3411.0
12	3411.0
13	3411.0
14	6099.0
15	8787.0
16	8787.0
17	8787.0
18	16347.5
19	23908.0
20	23908.0
21	23908.0
22	38467.5
23	53027.0
24	53027.0
25	53027.0
26	75995.0
27	98963.0
28	98963.0
29	98963.0
30	127659.0
31	156355.0
32	156355.0
33	156355.0
34	177269.0
35	198183.0
36	198183.0
37	198183.0
38	211416.5
39	224650.0
40	224650.0
41	224650.0
42	233326.0
43	242002.0
44	242002.0
45	242002.0
46	244388.5
47	246775.0
48	246775.0
49	246775.0
50	234710.5
51	222646.0
52	222646.0
53	222646.0
54	196432.0
55	170218.0
56	170218.0
57	170218.0
58	144874.0
59	119530.0
60	119530.0
61	119530.0
62	96296.0
63	73062.0
64	73062.0
65	73062.0
66	56174.0
67	39286.0
68	39286.0
69	39286.0
70	28700.5
71	18115.0
72	18115.0
73	18115.0
74	12639.0
75	7163.0
76	7163.0
77	7163.0
78	4847.0
79	2531.0
80	2531.0
81	2531.0
82	1699.5
83	868.0
84	868.0
85	868.0
86	561.0
87	254.0
88	254.0
89	254.0
90	168.0
91	82.0
92	82.0
93	82.0
94	49.0
95	16.0
96	16.0
97	16.0
98	16.5
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3609487167284721
2	0.04032040866381512
3	0.036293597422422434
4	0.03624130117253422
5	0.040791074912809075
6	0.017414651212776182
7	0.0177807249619937
8	0.024370052447909015
9	0.02138916620428066
10	0.020918499955286704
11	0.019349612458640204
12	0.020500129956180974
13	0.02002946370718702
14	0.028030789940084188
15	0.029338196187289604
16	0.021702943703609957
17	0.02311494245059181
18	0.09941517103750008
19	0.025520569945449784
20	0.022644276201597858
21	0.026409606193549467
22	0.020238648706739887
23	0.022644276201597858
24	0.01940190870852842
25	0.022591979951709643
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1912183.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.50696788613119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.79026947055027	63.174658844986986
2	10.739250068603505	16.00297919965147
3	2.5063841789917944	5.602292566033467
4	0.8284748097358654	2.4690858417383508
5	0.37212134432013005	1.3862816525501949
6	0.17971058979223958	0.8033814685468854
7	0.12465337674918125	0.6501281596844343
8	0.08349340898176776	0.497667259336655
9	0.06628027451163453	0.44445080560700817
>10	0.27666370723585254	3.5508310333098576
>50	0.016779956013932982	0.8634706610339333
>100	0.012609271385098273	1.89030097065344
>500	0.0023237217712761653	1.1279907463208168
>1k	9.858213575110841E-4	1.5364807905465585
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3573	0.18685450085059851	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3294	0.17226384713178602	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3116	0.16295511465168344	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3090	0.1615954121545898	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2788	0.14580194468834834	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2083	0.10893308851715552	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.229624988821676E-5	0.0
8	0.0	0.0	0.0	5.229624988821676E-5	0.0
9	0.0	0.0	0.0	5.229624988821676E-5	0.0
10	0.0	0.0	0.0	5.229624988821676E-5	0.0
11	0.0	0.0	0.0	5.229624988821676E-5	0.0
12	0.0	0.0	0.0	5.229624988821676E-5	5.229624988821676E-5
13	0.0	0.0	0.0	5.229624988821676E-5	5.229624988821676E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTCCG	40	0.0052968883	14.243016	9
TAGGACC	665	0.0	13.138822	4
CCAACGA	100	1.453318E-7	12.343301	19
GGCGAGG	755	0.0	11.69564	19
AGGACCG	65	8.047365E-4	11.688412	5
GGTATCA	980	0.0	11.37257	1
CCATGCG	60	0.005897975	11.077902	9
GTGCCGT	60	0.005897975	11.077902	11
TCCAACG	160	5.4205884E-10	10.690651	18
GACGTGG	880	0.0	10.465364	7
GCCGGTT	100	2.4121784E-5	10.444878	11
GGTCTAC	210	0.0	10.432955	1
GTCCTAA	955	0.0	10.373583	1
TGTAGGA	1990	0.0	10.260936	2
GTAGGAC	1960	0.0	10.223901	3
AGGACCT	1160	0.0	10.151789	5
GGACCTG	1105	0.0	10.138764	6
GGCGTGC	75	0.002659415	10.128102	8
CTGTAGG	1965	0.0	10.083228	1
GGACGTG	1940	0.0	10.081631	6
>>END_MODULE