FastQCFastQC Report
Thu 26 May 2016
SRR1512944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912183
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37200.19454204958416638No Hit
GTCCTACAGTGGACATTTCTAAATT33750.17649984337273159No Hit
CTGTAGGACGTGGAATATGGCAAGA32390.1693875533879341No Hit
GTCCTAAAGTGTGTATTTCTCATTT29920.15647037966554456No Hit
CTTTAGGACGTGAAATATGGCGAGG29240.15291423467314583No Hit
TATCAACGCAGAGTACTTTTTTTTT24280.1269752947285903No Hit
GGTATCAACGCAGAGTACTTTTTTT21960.114842564754524No Hit
GTCCTACAGTGTGCATTTCTCATTT20470.10705042352117972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC250.006029021519.00193810
TAGGACC6200.013.351934
ACCGTGC957.355084E-813.004738
CCAACGA802.8981214E-511.86254619
GGCGAGG8250.011.84816619
TCCTACG752.0582581E-411.4095272
TAGGACA6700.010.9353434
AGGACCT12850.010.88505655
TGCGTTG2100.010.84802512
TGTAGGA21000.010.6398352
TTAGGAC11550.010.5369373
AGGACGT18950.010.4943025
TGGCGAG17950.010.46784518
TCCAACG1553.7289283E-910.408154518
GTCCTAC17800.010.3083591
GGACGTG18700.010.2746466
GGACCTG12900.010.1752826
GAAATGT6100.010.1353936
GTAGGAC21050.010.0273743
GACGTGG9100.010.0264127
Produced by FastQC (version 0.11.5)