FastQCFastQC Report
Thu 26 May 2016
SRR1512943_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512943_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539279
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35680.14051232653048365No Hit
GTCCTACAGTGGACATTTCTAAATT29120.11467822165268174No Hit
CTGTAGGACGTGGAATATGGCAAGA25940.10215498178813749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT400.005301101614.2415667
TAGGACC6500.013.5882764
CCGTGCG604.1134242E-412.6594199
GGCGAGG6750.011.67456719
GGTATCA11000.011.6040441
TCCAACG1657.2759576E-1211.51922618
TGGCGAG15400.011.10782418
GTCCTAC16450.010.8865231
GTATTAG2450.010.497721
ATGGCGA16600.010.41934817
TATGGCG16150.010.35660416
AGGACCT12700.010.3197745
GTCCTAA9150.010.3065041
TCCTACA19200.010.2888172
GTAGGAC18550.010.1371553
GGCGAGA9400.010.10040219
GTATAAT3900.010.0142061
TCTAGAC2300.09.9101043
TTAGGAC10500.09.7685313
CCTACAG18800.09.7497673
Produced by FastQC (version 0.11.5)