FastQCFastQC Report
Thu 26 May 2016
SRR1512943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539279
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43290.1704814634390313No Hit
TATCAACGCAGAGTACTTTTTTTTT31290.12322395451622292No Hit
GTCCTACAGTGGACATTTCTAAATT27930.10999185201783655No Hit
GGTATCAACGCAGAGTACTTTTTTT26600.10475414477889196No Hit
CTGTAGGACGTGGAATATGGCAAGA26070.10266693813480127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACG500.001481396813.3220484
AACCGTG657.992112E-411.6982927
GGCGAGG6850.011.49846919
TAGGACC6550.011.1864524
CGTGCGC600.00587743911.08332310
GGTATCA12200.010.9102321
AGGGCGC803.77248E-410.687489510
TGGCGAG14550.010.43561518
AGGACGT14950.010.375035
GGACGTG15250.010.2307036
TCTATAC2600.09.87393953
GTATCAA22450.09.8674591
ATAGGAC3400.09.7878923
GTCCTAC16500.09.622731
GTAGGAC16150.09.596553
AGGGGCG900.00109727379.5157485
GAACCGT800.00446525269.5133076
TCGGAGC800.0044849269.5082383
TATTAGG2106.548362E-119.508052
GACGTGG7900.09.5048627
Produced by FastQC (version 0.11.5)