##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512943_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2539279
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.350747987913106	31.0	31.0	34.0	26.0	34.0
2	30.506355938043832	31.0	31.0	34.0	27.0	34.0
3	30.602326093351696	31.0	31.0	34.0	27.0	34.0
4	34.06187976980868	37.0	35.0	37.0	30.0	37.0
5	33.68944019148743	37.0	35.0	37.0	28.0	37.0
6	33.546643358213096	37.0	35.0	37.0	28.0	37.0
7	33.414205764707226	37.0	35.0	37.0	27.0	37.0
8	33.30015528029807	37.0	35.0	37.0	27.0	37.0
9	34.749358774675805	39.0	35.0	39.0	27.0	39.0
10	34.64311089880238	39.0	35.0	39.0	27.0	39.0
11	34.665630677054395	39.0	35.0	39.0	27.0	39.0
12	34.51079459956941	39.0	35.0	39.0	27.0	39.0
13	34.49147533610919	39.0	35.0	39.0	27.0	39.0
14	35.54033526839705	40.0	36.0	41.0	27.0	41.0
15	35.55071538023195	40.0	36.0	41.0	27.0	41.0
16	35.52411846039762	40.0	36.0	41.0	27.0	41.0
17	35.49828041739407	40.0	36.0	41.0	26.0	41.0
18	35.41975143337932	40.0	36.0	41.0	26.0	41.0
19	35.40852777501015	40.0	36.0	41.0	25.0	41.0
20	35.37420897821783	40.0	36.0	41.0	25.0	41.0
21	35.320870609334385	40.0	36.0	41.0	25.0	41.0
22	35.27671004249631	40.0	36.0	41.0	25.0	41.0
23	35.17243753049586	40.0	36.0	41.0	25.0	41.0
24	35.15514640179358	40.0	36.0	41.0	25.0	41.0
25	35.05612380522187	40.0	36.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6334411531385982
1101	2	0.6696034011655279
1101	3	0.6338684486834971
1101	4	0.4326264646006095
1101	5	0.688684781064886
1101	6	0.7558420057362554
1101	7	0.8479259013731166
1101	8	0.7140338697720949
1101	9	0.6179084054840871
1101	10	1.0112638426168132
1101	11	1.0214986682192944
1101	12	1.0104357515573454
1101	13	1.0290624212956985
1101	14	1.019538591717719
1101	15	1.0256819218867221
1101	16	1.1619534288061715
1101	17	1.1935056546948175
1101	18	0.6423562538623173
1101	19	0.8754111490324235
1101	20	0.9187237538880879
1101	21	1.0490045503441934
1101	22	1.0561539260056563
1101	23	0.9991686861079145
1101	24	0.9776194121250654
1101	25	1.0009701920497776
1102	1	0.5925980322478424
1102	2	0.5716351044343284
1102	3	0.5275844874776006
1102	4	0.5003832824235843
1102	5	0.6924404221253724
1102	6	0.6829826516847888
1102	7	0.7542513573479468
1102	8	0.808007968343972
1102	9	1.0173223073457223
1102	10	1.0440903722017438
1102	11	0.9983406811158204
1102	12	1.0843335751243899
1102	13	1.071420438217089
1102	14	1.1933011343609152
1102	15	1.2276179272429388
1102	16	1.176949654803984
1102	17	1.1171586494975898
1102	18	1.1525296929190603
1102	19	1.2180814013679324
1102	20	1.2481582477179245
1102	21	1.1258289448121488
1102	22	1.138131676447287
1102	23	1.2264631253962648
1102	24	1.191383558444116
1102	25	1.162202332244604
1103	1	0.6204597775346166
1103	2	0.4845607677477908
1103	3	0.556075630756748
1103	4	0.6039797195257108
1103	5	0.5777118103698697
1103	6	0.6274669000728608
1103	7	0.6088332779846795
1103	8	0.630323970210128
1103	9	0.7408123434003926
1103	10	0.7504248786913692
1103	11	0.7381332538970184
1103	12	0.7968959505616411
1103	13	0.8270347650568581
1103	14	0.9806344863271548
1103	15	0.8262170732826277
1103	16	0.9907298159212701
1103	17	1.0795879967511368
1103	18	1.1700270646046462
1103	19	1.081528105171408
1103	20	1.1271155002532538
1103	21	0.7951947846795377
1103	22	0.9609921532632981
1103	23	1.067840587895759
1103	24	1.069170431706688
1103	25	1.158256635692915
1104	1	0.47238230846893003
1104	2	0.5570800247356971
1104	3	0.499281466929002
1104	4	0.5575696960570653
1104	5	0.575652107278863
1104	6	0.6582332138041025
1104	7	0.5460446418674536
1104	8	0.5229409297201499
1104	9	0.7734918965709383
1104	10	0.7552336581335481
1104	11	0.7330937776351263
1104	12	0.7407567642315058
1104	13	0.7802381703301364
1104	14	0.8803876056052999
1104	15	0.9055494747271666
1104	16	0.8955114101191342
1104	17	0.938061826943958
1104	18	0.8736272472004174
1104	19	0.9414091130640116
1104	20	1.0335178042259017
1104	21	0.8547276199745966
1104	22	0.9134032630813493
1104	23	0.8337400457018944
1104	24	0.9894330350696023
1104	25	1.2497967493699136
1105	1	0.47642828721869535
1105	2	0.5171817880838461
1105	3	0.5293893572972692
1105	4	0.46846802761983497
1105	5	0.3812425503572001
1105	6	0.5253381016250813
1105	7	0.6814409937427399
1105	8	0.7242876182610303
1105	9	0.8577997457435487
1105	10	0.7855197272442638
1105	11	0.691409763506158
1105	12	0.6071911923938487
1105	13	0.8027147907477286
1105	14	0.9583404783744882
1105	15	0.7679481936193895
1105	16	0.8172254660736726
1105	17	0.6704253856234743
1105	18	0.8220406420293997
1105	19	0.9549357184404172
1105	20	0.923512577256993
1105	21	0.8092762766710635
1105	22	0.9370539401245921
1105	23	0.9111251536089426
1105	24	0.9515628571217576
1105	25	1.0077930600757057
1106	1	0.621706689010697
1106	2	0.6073431213101941
1106	3	0.5639596664210274
1106	4	0.5393214778329636
1106	5	0.6648975860638302
1106	6	0.6540219320011147
1106	7	0.7813553747199151
1106	8	0.7219813743547903
1106	9	0.660922176173095
1106	10	0.7384660304297768
1106	11	0.7130007989609695
1106	12	0.8462731222004365
1106	13	0.7683535948317441
1106	14	0.9615491599395796
1106	15	0.9972488886079773
1106	16	0.9804857976980657
1106	17	0.9592856115689372
1106	18	1.0852574739784941
1106	19	1.0323341798522563
1106	20	1.1258518587280122
1106	21	1.1632349535275566
1106	22	1.1436552738566306
1106	23	1.0948198834142957
1106	24	1.034333502632684
1106	25	1.108356388611
1107	1	0.3822438140402227
1107	2	0.41339556203248407
1107	3	0.36458535981878626
1107	4	0.39442337279875517
1107	5	0.4744579482528408
1107	6	0.40210764371553864
1107	7	0.3452913430214437
1107	8	0.4977123079174177
1107	9	0.32730600083726813
1107	10	0.5972517408081401
1107	11	0.5024840425890247
1107	12	0.44877108092315154
1107	13	0.36487273494164185
1107	14	0.5695300184432028
1107	15	0.6538730256184593
1107	16	0.4366027475722589
1107	17	0.5608298608389575
1107	18	0.6279075648937891
1107	19	0.6582874796630165
1107	20	0.7155290331515332
1107	21	0.6470263043602884
1107	22	0.6326139111127702
1107	23	0.6815744201672445
1107	24	0.7257213669965878
1107	25	0.7389325922209409
1108	1	0.3068643996832243
1108	2	0.3396072826177772
1108	3	0.3392303501798004
1108	4	0.39569276928236974
1108	5	0.4083031661571255
1108	6	0.410773513278464
1108	7	0.3024773034612167
1108	8	0.5533291678905243
1108	9	0.37937978736095346
1108	10	0.5013469051625066
1108	11	0.4270914602187119
1108	12	0.572619318691828
1108	13	0.4684988546617035
1108	14	0.6310764348122788
1108	15	0.542517721015976
1108	16	0.5837631971809074
1108	17	0.39787569605970674
1108	18	0.4781209744962567
1108	19	0.6021346815479731
1108	20	0.5456840470042863
1108	21	0.6497437433748345
1108	22	0.7444384359230654
1108	23	0.6323150770228665
1108	24	0.619197845413332
1108	25	0.6123339954044411
1109	1	0.17598490606751938
1109	2	0.24632804250995477
1109	3	0.3017174341617981
1109	4	0.48364365862245506
1109	5	0.4309833500190976
1109	6	0.08303709985996477
1109	7	0.3596911891545247
1109	8	0.4754970142498749
1109	9	0.5852712357730141
1109	10	0.41195284655624675
1109	11	0.4277423536837759
1109	12	0.4842791664139483
1109	13	0.6147989534065417
1109	14	0.6065731763345568
1109	15	0.5881759401358053
1109	16	0.4273580490050932
1109	17	0.5774307535624033
1109	18	0.5817124677738192
1109	19	0.7045989992181063
1109	20	0.679493165954888
1109	21	0.6905470929585391
1109	22	0.8561512335898556
1109	23	0.7936884679632925
1109	24	0.38080144575494046
1109	25	0.5583174766513253
1110	1	0.11343295700531186
1110	2	0.13513266929733803
1110	3	0.11170407035587004
1110	4	0.23558665094373765
1110	5	0.18922978845296967
1110	6	0.15576955706851692
1110	7	0.15611476876944863
1110	8	0.29546195468077485
1110	9	0.31477392977252094
1110	10	0.30201411597454353
1110	11	0.14051025501329661
1110	12	0.310679507031395
1110	13	-0.07246090588768794
1110	14	0.24708679688541224
1110	15	0.3965438140188766
1110	16	0.32970908102418406
1110	17	0.3239834847980916
1110	18	0.18607450501401956
1110	19	0.2628447213298628
1110	20	0.3023439168489759
1110	21	0.29563123315961803
1110	22	0.33610062354731696
1110	23	0.3883380570080419
1110	24	0.2865962277630416
1110	25	0.3726398723743003
1111	1	-0.33657715784054076
1111	2	-0.11788932775191796
1111	3	-0.12201264719228888
1111	4	-0.024427046450966827
1111	5	0.08691737524507204
1111	6	-0.04774263630341835
1111	7	0.25149365445226124
1111	8	0.2380772701518552
1111	9	-0.21529930071491776
1111	10	-0.1272335338185968
1111	11	0.06782686613135525
1111	12	0.09274294555082463
1111	13	0.21897234285630418
1111	14	0.3597078823030415
1111	15	0.477049018181809
1111	16	0.1988617160411863
1111	17	0.47916801596004177
1111	18	0.3905856016975022
1111	19	0.39040999322737946
1111	20	0.29905285493465783
1111	21	0.25318438219016315
1111	22	0.0855947566875912
1111	23	0.2241779703890856
1111	24	0.33253494275907514
1111	25	0.32818138783731143
1112	1	-0.0794797662525184
1112	2	-0.3592145449333799
1112	3	-0.08377714058368113
1112	4	-0.29662392156036077
1112	5	-0.09810179005216924
1112	6	-0.07454550125405035
1112	7	-0.19075808611523115
1112	8	-0.06090161512523906
1112	9	-0.27652807445616645
1112	10	-0.11989078819208032
1112	11	-0.18344521355973598
1112	12	-0.09244640967142459
1112	13	-0.21839962677615432
1112	14	-0.05646817973298823
1112	15	-0.122332463714379
1112	16	-0.31382072296540287
1112	17	-0.15115799146872888
1112	18	-0.0844335958750051
1112	19	-0.08949530619640456
1112	20	0.09272146697188788
1112	21	0.15817396348818846
1112	22	0.020540371634311327
1112	23	0.15663280426708326
1112	24	0.17599819725222687
1112	25	0.19972312483714916
1113	1	-0.219752836971562
1113	2	-0.5899350130072811
1113	3	-0.20848640332002688
1113	4	-0.25535086326625134
1113	5	-0.19927348528277378
1113	6	-0.33835258253818523
1113	7	-0.06362291676709475
1113	8	0.09298722950491367
1113	9	-0.020413730021033416
1113	10	-0.35071759904640487
1113	11	-0.030773281159149235
1113	12	-0.13387258695178872
1113	13	-0.014925324088785885
1113	14	-0.0637550355460661
1113	15	0.04516642961419137
1113	16	-0.42398392563476506
1113	17	-0.17945857049481617
1113	18	-0.1436607993571002
1113	19	-0.09516655571311361
1113	20	0.014660262742829389
1113	21	-0.15658227279109838
1113	22	-0.351154322993807
1113	23	-0.017211451919372678
1113	24	-0.33321220821220976
1113	25	-0.37462753250253655
1114	1	-0.7201396461286009
1114	2	-0.587313025328779
1114	3	-0.5965651620024452
1114	4	-0.4311729773265114
1114	5	-0.6101700076876995
1114	6	-0.8777969944472801
1114	7	-0.721612044176247
1114	8	-1.0992735507216906
1114	9	-0.8708158307924165
1114	10	-0.9469492102475314
1114	11	-0.7988790224915547
1114	12	-0.9231367891741655
1114	13	-0.8703996858717531
1114	14	-1.8790619776497692
1114	15	-1.0574677768956278
1114	16	-1.099641193923425
1114	17	-1.263322772283395
1114	18	-1.332959778253226
1114	19	-1.4857950012970136
1114	20	-0.6845634438143406
1114	21	-0.6898406302186615
1114	22	-0.8751552889321914
1114	23	-0.8070338161403185
1114	24	-1.5122318241360233
1114	25	-1.388878738660253
1115	1	-1.541584505471672
1115	2	-1.304591573641222
1115	3	-0.9768091591990071
1115	4	-2.3485080278825166
1115	5	-1.441064434450226
1115	6	-1.289108750872856
1115	7	-1.1346682634848548
1115	8	-1.035238784468703
1115	9	-1.3711318777322532
1115	10	-1.2560597036718022
1115	11	-1.16551108804142
1115	12	-1.6431299754902824
1115	13	-1.131650769275339
1115	14	-1.9764483826554766
1115	15	-1.960925334049513
1115	16	-1.3710119538489423
1115	17	-1.4297500883275447
1115	18	-1.3291353136655601
1115	19	-1.1877643714392576
1115	20	-1.2135991441037817
1115	21	-1.3089239815002642
1115	22	-1.493753764521756
1115	23	-1.8926641950976162
1115	24	-1.3736729957603444
1115	25	-1.2517363405365103
1116	1	-0.9446980050481919
1116	2	-0.9661474400276049
1116	3	-0.8174099891962676
1116	4	-0.9560389488078087
1116	5	-0.9708822516934887
1116	6	-1.519528225444411
1116	7	-0.9953627672661796
1116	8	-1.0955753045141137
1116	9	-1.184641106449753
1116	10	-1.1923381638451147
1116	11	-1.1696457406874003
1116	12	-1.773741229515302
1116	13	-1.4552947745668234
1116	14	-1.7233358357111683
1116	15	-1.613681968446265
1116	16	-1.7607291559664304
1116	17	-1.671339618962513
1116	18	-2.2665898884142592
1116	19	-1.8232020950682326
1116	20	-1.6266027968904524
1116	21	-1.6209226444966944
1116	22	-2.9588316123098934
1116	23	-2.9255978062448165
1116	24	-2.0805592793384164
1116	25	-2.4501551541586792
1201	1	0.7458050204587892
1201	2	0.7273038126287581
1201	3	0.6441683267599352
1201	4	0.6834942285258236
1201	5	0.818830627947122
1201	6	0.8123665985641182
1201	7	0.8296694062228696
1201	8	0.9004645388076611
1201	9	1.0395566889180117
1201	10	0.8651427772029763
1201	11	0.9813068209666866
1201	12	0.9175319095097478
1201	13	0.9100273450447887
1201	14	0.9077813756151016
1201	15	1.0209475853510241
1201	16	1.02125201826356
1201	17	0.9829252972808575
1201	18	1.0048806488706532
1201	19	0.7962233762060293
1201	20	1.0948348606171692
1201	21	0.9919183287398283
1201	22	1.0696275022651918
1201	23	1.012409611520809
1201	24	1.1397225366894546
1201	25	0.9820774495346569
1202	1	0.6494963403795992
1202	2	0.7761870311688064
1202	3	0.7539102629464551
1202	4	0.7681855812567875
1202	5	0.8295046806191309
1202	6	0.9627255135167161
1202	7	0.9119969897430238
1202	8	0.8027256375942287
1202	9	0.9990592377323537
1202	10	1.0064158151166467
1202	11	1.080651159102743
1202	12	1.0332374157446722
1202	13	1.0927934406664406
1202	14	1.034072573158845
1202	15	1.163909078770395
1202	16	1.0127988496832998
1202	17	1.1200543927529765
1202	18	1.1391051213926957
1202	19	1.3230338234782337
1202	20	1.4442556244826577
1202	21	1.3804812585724804
1202	22	1.3458111489605713
1202	23	1.2565506880403063
1202	24	1.1503531621488534
1202	25	1.2048823392196013
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2211	7	0.07276790176471337
2211	8	0.0785188782959878
2211	9	-0.29408831558027515
2211	10	-0.3552421792313325
2211	11	-0.12338764597635077
2211	12	-0.07349486327419186
2211	13	-0.09691978873153317
2211	14	-1.2226001382545348
2211	15	-0.6423294842461047
2211	16	-0.3926808635324619
2211	17	-0.11916063042634306
2211	18	-0.3062018038844556
2211	19	-0.20978069669296673
2211	20	-0.3367529229021784
2211	21	-0.33157081313149916
2211	22	-0.19288266497590456
2211	23	-0.28416888551706165
2211	24	-0.38794598028152194
2211	25	-0.4777520950316472
2212	1	-0.26534790960557686
2212	2	-0.22769515404947072
2212	3	-0.14446265430379412
2212	4	-0.05250082139556156
2212	5	-0.1392325402070398
2212	6	-0.21739559982535184
2212	7	-0.10070755371332751
2212	8	-0.3872780581428472
2212	9	-0.40020668818523575
2212	10	-0.37276489160233695
2212	11	-0.355155664260451
2212	12	-0.17121128302873245
2212	13	-0.2885364675075266
2212	14	-0.27535576072314427
2212	15	-0.27254935466646657
2212	16	-0.10784907487663276
2212	17	-0.3363579538713779
2212	18	-0.09236641836484694
2212	19	-0.1681469491812848
2212	20	-0.30688312769746773
2212	21	-0.13033337286591973
2212	22	-0.18576037505302878
2212	23	-0.538192713816656
2212	24	-0.32893633367695685
2212	25	-0.5206296328216098
2213	1	-0.23972415944934866
2213	2	-0.36204359486936966
2213	3	-0.184153005509895
2213	4	-0.2679348296445312
2213	5	-0.16829531361036487
2213	6	-0.025241884664751524
2213	7	-0.38761114388955065
2213	8	-0.06525385804285833
2213	9	-0.36684757682415636
2213	10	-0.1580085330221621
2213	11	-0.11005153665139744
2213	12	-0.3582855549947368
2213	13	-0.1291774811308244
2213	14	-0.11678573822595695
2213	15	-0.39978540328539225
2213	16	-0.7056259152715825
2213	17	-0.5084301948412531
2213	18	-0.7051452897559187
2213	19	-0.8928141782776464
2213	20	-0.429465461155786
2213	21	-1.1414862756280755
2213	22	-0.6151461549555464
2213	23	-0.6813935637104223
2213	24	-0.7949327716222143
2213	25	-0.7123537141864418
2214	1	-0.9121031489239257
2214	2	-0.8842151528865152
2214	3	-0.6926969533920762
2214	4	-1.2293318884680602
2214	5	-1.2096967226588333
2214	6	-2.641362385895544
2214	7	-1.2734376335763358
2214	8	-1.839818039776155
2214	9	-1.225200008706004
2214	10	-1.492680091039304
2214	11	-1.3032267233927612
2214	12	-1.2244630866134472
2214	13	-1.4380195785758687
2214	14	-1.5341367695475725
2214	15	-1.996648201434951
2214	16	-1.4715042362969655
2214	17	-1.6240374225808338
2214	18	-1.5772168456510087
2214	19	-3.2685860779466083
2214	20	-1.8483947753056924
2214	21	-2.2905517730391622
2214	22	-1.9895214732646878
2214	23	-2.0627730094281063
2214	24	-1.3926616358951946
2214	25	-1.1637041407206894
2215	1	-1.518540828891016
2215	2	-1.3060041482049236
2215	3	-1.2081324982393866
2215	4	-1.1304993484590469
2215	5	-1.8331859785010316
2215	6	-1.6561613124591759
2215	7	-1.884390406293818
2215	8	-2.655477870141869
2215	9	-1.9764879364645225
2215	10	-1.3638530357416627
2215	11	-2.2367148039396696
2215	12	-2.7141449733541556
2215	13	-2.1176438758472216
2215	14	-2.099365658305871
2215	15	-3.2663441917191776
2215	16	-2.714790361936032
2215	17	-2.369385212046275
2215	18	-3.0738182834289134
2215	19	-3.7227446644581335
2215	20	-3.216903717344046
2215	21	-2.3168196228364195
2215	22	-3.317355404039791
2215	23	-3.380905856972717
2215	24	-2.1848449427843875
2215	25	-2.506689586647312
2216	1	-1.2283077056129095
2216	2	-1.0832969246326485
2216	3	-1.4745615708968316
2216	4	-1.026250655975062
2216	5	-0.9494097201572984
2216	6	-1.564638648272151
2216	7	-2.749313545438408
2216	8	-1.8102789514135402
2216	9	-1.9945045219551503
2216	10	-1.8008407884733586
2216	11	-2.3084994207069798
2216	12	-1.8674475531273274
2216	13	-2.2016169961525236
2216	14	-2.247006944355462
2216	15	-2.568878162938269
2216	16	-2.2569488954440473
2216	17	-2.4379576430754497
2216	18	-2.2624652765741757
2216	19	-2.5005697805185463
2216	20	-2.771790871712163
2216	21	-2.652974817123102
2216	22	-3.0442556664071816
2216	23	-2.0072898535989268
2216	24	-2.196785424448052
2216	25	-2.023955193013265
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72251.0
3	0.0
4	1137.0
5	10126.0
6	11470.0
7	8956.0
8	7145.0
9	6469.0
10	5527.0
11	5001.0
12	4649.0
13	4356.0
14	4166.0
15	4235.0
16	4241.0
17	4555.0
18	4986.0
19	5882.0
20	7495.0
21	9557.0
22	12133.0
23	14907.0
24	17579.0
25	20307.0
26	23184.0
27	25588.0
28	28977.0
29	34776.0
30	43461.0
31	55395.0
32	70531.0
33	91123.0
34	118591.0
35	155585.0
36	213100.0
37	332321.0
38	1099517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.3495635682449	19.370459952996928	13.753620509513926	27.526355969244243
2	13.74986413450133	20.41097541188906	39.747274419952774	26.091886033656834
3	16.744241180272038	24.33875915171196	30.203888584121714	28.713111083894287
4	11.499324206410328	17.247962081499406	37.41312280455718	33.83959090753308
5	12.953864707644286	37.84891144987625	35.901502372582094	13.295721469897373
6	32.09768717371067	37.38480602165659	17.673442265262832	12.844064539369915
7	27.891041220416497	31.58330381860795	22.213773809913086	18.31188115106247
8	25.13552316968348	36.79262422907958	20.351353300577877	17.72049930065906
9	25.9693841364908	15.058197722526497	19.989085464360297	38.9833326766224
10	14.46438695443388	29.162214822413794	34.60346585438743	21.769932368764888
11	34.17779347865043	22.445578950643466	24.52061468128738	18.85601288941873
12	22.712155204466825	24.81511649157744	31.683298567566375	20.78942973638936
13	28.450846340192566	21.76117685377036	25.9660376927497	23.821939113287378
14	20.886043544016637	21.758420505387864	27.59279097611696	29.762744974478544
15	22.86918441960676	30.463718308683163	23.402648929939904	23.264448341770173
16	22.456592151550613	28.252511354912556	26.974963062807213	22.31593343072962
17	21.76541150192342	28.41928818627152	27.622698147881874	22.19260216392319
18	21.699513868760317	27.747602424670138	29.00006773701655	21.552815969553
19	23.31891652613496	27.07700087351516	27.431999325762867	22.17208327458701
20	23.318970049777224	27.313987207343665	27.75973341871748	21.607309324161626
21	22.952353610428826	26.765852692251197	27.217006154133333	23.06478754318664
22	23.01200705560721	27.578106509108768	27.246513448619027	22.163372986664996
23	22.414709057177255	27.37332132467523	27.443301819138426	22.768667799009087
24	22.799609022414263	26.890223827579202	27.997744238909046	22.312422911097492
25	22.744073154699947	27.08297642494566	27.64264815980989	22.530302260544502
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	131.0
1	131.0
2	308.0
3	485.0
4	485.0
5	485.0
6	1172.0
7	1859.0
8	1859.0
9	1859.0
10	2953.0
11	4047.0
12	4047.0
13	4047.0
14	7344.0
15	10641.0
16	10641.0
17	10641.0
18	19481.5
19	28322.0
20	28322.0
21	28322.0
22	45318.5
23	62315.0
24	62315.0
25	62315.0
26	88906.5
27	115498.0
28	115498.0
29	115498.0
30	148649.0
31	181800.0
32	181800.0
33	181800.0
34	209791.5
35	237783.0
36	237783.0
37	237783.0
38	259826.0
39	281869.0
40	281869.0
41	281869.0
42	298363.0
43	314857.0
44	314857.0
45	314857.0
46	322878.5
47	330900.0
48	330900.0
49	330900.0
50	321387.0
51	311874.0
52	311874.0
53	311874.0
54	281613.5
55	251353.0
56	251353.0
57	251353.0
58	216758.0
59	182163.0
60	182163.0
61	182163.0
62	148825.5
63	115488.0
64	115488.0
65	115488.0
66	89020.0
67	62552.0
68	62552.0
69	62552.0
70	45535.5
71	28519.0
72	28519.0
73	28519.0
74	19813.5
75	11108.0
76	11108.0
77	11108.0
78	7580.0
79	4052.0
80	4052.0
81	4052.0
82	2637.5
83	1223.0
84	1223.0
85	1223.0
86	793.5
87	364.0
88	364.0
89	364.0
90	216.0
91	68.0
92	68.0
93	68.0
94	37.5
95	7.0
96	7.0
97	7.0
98	4.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07868375235647598
2	1.1814377230702101E-4
3	0.0
4	0.03079614331469681
5	0.07714788331648471
6	0.08021962139646727
7	0.039302494920802324
8	0.07443057655342324
9	0.054306754003793985
10	0.11329987764243314
11	0.005592138555865661
12	0.015083021597863015
13	0.006694813764064524
14	0.009412120527126007
15	0.008506351606105514
16	0.02008444129219357
17	0.032843968701351846
18	0.0018509190994766625
19	0.004450082090231125
20	0.0065372887343218295
21	3.9381257435673665E-4
22	8.663876635848207E-4
23	0.0
24	1.1814377230702101E-4
25	0.0044107008327954516
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2539279.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.59014196756328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.07932429332004	55.727315462386805
2	13.205133055431167	18.378941680560352
3	3.6312638847302314	7.581005077801864
4	1.2941618703276505	3.6024363314043373
5	0.6023654314612434	2.0959347945870217
6	0.3405889395857342	1.4220979595011844
7	0.19208819195201235	0.935721118376316
8	0.1347184818913351	0.750006262437809
9	0.09002633549002703	0.5638450920813415
>10	0.3979729003977258	4.6120636612575225
>50	0.018216991373423325	0.8616237861658694
>100	0.011646953412112143	1.5324263630796828
>500	0.0016995481550036592	0.7942739673130649
>1k	7.9312247229801E-4	1.142308443046781
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4329	0.1704814634390313	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3129	0.12322395451622292	No Hit
GTCCTACAGTGGACATTTCTAAATT	2793	0.10999185201783655	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2660	0.10475414477889196	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2607	0.10266693813480127	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	3.938125743567367E-5	0.0	0.0	0.0
8	0.0	3.938125743567367E-5	0.0	0.0	0.0
9	0.0	3.938125743567367E-5	0.0	0.0	0.0
10	0.0	3.938125743567367E-5	0.0	0.0	3.938125743567367E-5
11	3.938125743567367E-5	3.938125743567367E-5	0.0	0.0	3.938125743567367E-5
12	3.938125743567367E-5	3.938125743567367E-5	0.0	0.0	3.938125743567367E-5
13	3.938125743567367E-5	3.938125743567367E-5	0.0	3.938125743567367E-5	3.938125743567367E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAACG	50	0.0014813968	13.322048	4
AACCGTG	65	7.992112E-4	11.698292	7
GGCGAGG	685	0.0	11.498469	19
TAGGACC	655	0.0	11.186452	4
CGTGCGC	60	0.005877439	11.083323	10
GGTATCA	1220	0.0	10.910232	1
AGGGCGC	80	3.77248E-4	10.6874895	10
TGGCGAG	1455	0.0	10.435615	18
AGGACGT	1495	0.0	10.37503	5
GGACGTG	1525	0.0	10.230703	6
TCTATAC	260	0.0	9.8739395	3
GTATCAA	2245	0.0	9.867459	1
ATAGGAC	340	0.0	9.787892	3
GTCCTAC	1650	0.0	9.62273	1
GTAGGAC	1615	0.0	9.59655	3
AGGGGCG	90	0.0010972737	9.515748	5
GAACCGT	80	0.0044652526	9.513307	6
TCGGAGC	80	0.004484926	9.508238	3
TATTAGG	210	6.548362E-11	9.50805	2
GACGTGG	790	0.0	9.504862	7
>>END_MODULE