FastQCFastQC Report
Thu 26 May 2016
SRR1512942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000869
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT29570.1477857870755157No Hit
GTCCTAAAGTGTGTATTTCTCATTT26460.1322425406161023No Hit
GTATCAACGCAGAGTACTTTTTTTT26370.13179273605618358No Hit
CTTTAGGACGTGAAATATGGCGAGG23420.1170491421477368No Hit
CTGTAGGACGTGGAATATGGCAAGA22440.11215127027306634No Hit
GTCCTACAGTGTGCATTTCTCATTT21160.1057540498653335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC1300.014.6076558
GCGTGCG1001.0146323E-813.293639
ACCGCCC500.001505800913.2929658
CGTGCGC802.0110056E-613.05624510
CGAAATC1155.305992E-912.39466613
TAGGACC6900.011.7005114
GCGCCAC1251.825174E-811.40309213
GGGCCGC855.35453E-511.17027956
TGTAGGA17800.011.0457752
GTATAGA2800.010.8870521
AAAAGCG700.001494267510.8549285
GCCGGTT1158.8739944E-710.73398711
GTAGGAC17400.010.6446463
AGGACGT14750.010.5605575
GGACGTG14600.010.4702256
GTATCAA17100.010.4175081
TGGCGAG15800.010.40393918
CGGTTTC1201.5187525E-610.29445813
GGCGAGG7250.010.21502919
TAGGACA6250.010.1819224
Produced by FastQC (version 0.11.5)