##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512942_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2000869
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.33009157521057	31.0	29.0	33.0	16.0	34.0
2	28.286775895873244	31.0	28.0	34.0	16.0	34.0
3	28.54722672998582	31.0	28.0	34.0	16.0	34.0
4	31.905585023307374	35.0	32.0	37.0	19.0	37.0
5	32.08045554206697	35.0	33.0	37.0	19.0	37.0
6	32.04400438009685	35.0	33.0	37.0	19.0	37.0
7	31.87757619314408	35.0	32.0	37.0	17.0	37.0
8	31.877362286086694	35.0	33.0	37.0	17.0	37.0
9	33.141882352118	38.0	33.0	39.0	17.0	39.0
10	33.00267683691436	37.0	33.0	39.0	17.0	39.0
11	33.068376790284624	38.0	33.0	39.0	17.0	39.0
12	32.95843256105222	38.0	33.0	39.0	16.0	39.0
13	32.95410594096865	38.0	33.0	39.0	15.0	39.0
14	33.857343984038934	39.0	33.0	41.0	11.0	41.0
15	33.795478364650556	39.0	33.0	41.0	11.0	41.0
16	33.684015795137014	39.0	33.0	41.0	10.0	41.0
17	33.595710663716616	39.0	33.0	41.0	10.0	41.0
18	33.30446820856338	38.0	32.0	41.0	10.0	41.0
19	33.526496237384855	39.0	33.0	41.0	9.0	41.0
20	33.6123634280905	39.0	33.0	41.0	2.0	41.0
21	33.53376357972461	39.0	33.0	41.0	2.0	41.0
22	33.42605937720061	39.0	33.0	41.0	2.0	41.0
23	33.145271879368416	39.0	33.0	41.0	2.0	41.0
24	33.142108253963656	39.0	33.0	41.0	2.0	41.0
25	33.03583842820294	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7334620808216847
1101	2	0.9010244963956389
1101	3	0.9670832759044679
1101	4	1.0157335517776538
1101	5	0.7482130849146529
1101	6	1.0036692531930598
1101	7	1.0543598294668826
1101	8	0.8138252858169288
1101	9	0.8136420147089751
1101	10	0.8123570104035025
1101	11	1.0164211031426404
1101	12	1.1462448623797314
1101	13	1.000438416532667
1101	14	0.9709831962243385
1101	15	0.6459841299735771
1101	16	1.3051341663551028
1101	17	1.2380768110711884
1101	18	1.5317276318170912
1101	19	0.9708304885424752
1101	20	1.2140723273290561
1101	21	1.2445144976520623
1101	22	1.213272523001251
1101	23	1.4438576533469458
1101	24	1.0191778614467282
1101	25	1.2004872359359098
1102	1	0.9197525590879039
1102	2	0.9987317278717818
1102	3	0.9592041351544687
1102	4	1.0451496142880181
1102	5	0.9464218350654079
1102	6	0.8994718950571112
1102	7	0.9741558425909957
1102	8	1.012935794040903
1102	9	1.0881701841781606
1102	10	1.253752614541277
1102	11	1.183980472226878
1102	12	1.233181523419539
1102	13	1.1024130228277471
1102	14	1.3061736593017557
1102	15	1.337115885235427
1102	16	1.2195703632925614
1102	17	1.0745923841233704
1102	18	1.486135081164072
1102	19	1.4577834497624522
1102	20	1.430832720031681
1102	21	1.1857510103849265
1102	22	1.5443831044937113
1102	23	1.720167831780408
1102	24	1.6461988676864863
1102	25	1.54742555992339
1103	1	0.5811495847013006
1103	2	0.7122558619877744
1103	3	0.6819123114401151
1103	4	0.7515435502421433
1103	5	0.3750981698741569
1103	6	0.6584402006687782
1103	7	0.6212256225257207
1103	8	0.6260106285462861
1103	9	0.8325455454044928
1103	10	0.9464083585496184
1103	11	0.9456483326558214
1103	12	1.1164657472915351
1103	13	1.0387510531359894
1103	14	1.34038633977503
1103	15	1.2804185391448257
1103	16	1.4188073213731158
1103	17	1.2594975999318763
1103	18	1.390374029736634
1103	19	1.4699928998830458
1103	20	1.3351722923716736
1103	21	1.1804375358732173
1103	22	1.3820068207662644
1103	23	1.5850617881190843
1103	24	1.3653392469351715
1103	25	1.3337283569243823
1104	1	0.7515330098577273
1104	2	0.6900099238386304
1104	3	0.6661272437202577
1104	4	1.022705118926499
1104	5	0.7193769971463269
1104	6	0.7877847762189703
1104	7	0.997875638534957
1104	8	0.8858525629667717
1104	9	0.951148948510756
1104	10	1.0675235556524996
1104	11	1.0980375337554875
1104	12	1.0117207831033284
1104	13	1.1380024044574952
1104	14	1.272352210921973
1104	15	1.3632393271778014
1104	16	1.0343780388235047
1104	17	1.0535922026898774
1104	18	1.2822541134578458
1104	19	1.2981268459311295
1104	20	1.3567293601735528
1104	21	1.2787297131136057
1104	22	1.2535076590824374
1104	23	1.387492037531949
1104	24	1.2463937745522813
1104	25	0.9767154832900999
1105	1	0.8360564355878992
1105	2	0.8706545576507914
1105	3	0.8914939197898661
1105	4	1.0786035547312558
1105	5	0.6433718250104548
1105	6	0.5767757369931914
1105	7	0.8573561310425646
1105	8	0.9564281161388699
1105	9	0.8052126090252116
1105	10	1.0620427888062451
1105	11	0.8609560551787041
1105	12	1.045386551840508
1105	13	1.0115661951665587
1105	14	1.0300030816759218
1105	15	1.1886909784794426
1105	16	1.194272131708054
1105	17	1.1414237727128764
1105	18	1.2160966523767343
1105	19	1.1990452604087665
1105	20	1.3211765445601173
1105	21	1.1861603767341293
1105	22	0.9690208118379147
1105	23	1.1365930747490083
1105	24	1.2472654197161717
1105	25	1.314394772117538
1106	1	0.6315333147364903
1106	2	0.7124032690387843
1106	3	0.7579684534791902
1106	4	0.7137872978246698
1106	5	0.7029790146620059
1106	6	0.7497417695515054
1106	7	0.5667895743868954
1106	8	0.6748416709775036
1106	9	0.8250018421620098
1106	10	0.9903737890411008
1106	11	0.847064462367733
1106	12	1.0522135688118652
1106	13	0.8700772920627671
1106	14	1.1588696902468882
1106	15	1.2143349754634087
1106	16	1.1452901969977347
1106	17	1.188708803182216
1106	18	1.3530356271730852
1106	19	0.9480355386500747
1106	20	0.9968086442869435
1106	21	0.9392540498654895
1106	22	0.9834782969122671
1106	23	1.2078066567813721
1106	24	1.1324093765275833
1106	25	1.2912055501054525
1107	1	0.5780521678670851
1107	2	0.6256831440484625
1107	3	0.7382711528587471
1107	4	0.8523158303093261
1107	5	0.7229456677310395
1107	6	0.7026005649249214
1107	7	0.8653428329833517
1107	8	0.8950254045934152
1107	9	1.0141467747728612
1107	10	0.8710154083375627
1107	11	0.9600389878085878
1107	12	1.075427281538282
1107	13	1.0084033046334469
1107	14	1.1764526174810896
1107	15	1.3456148743556042
1107	16	1.2379584175689757
1107	17	1.1444264709694352
1107	18	1.5273897687294067
1107	19	1.4042191081699684
1107	20	1.32859789568937
1107	21	1.2774658693244305
1107	22	1.384942969465591
1107	23	1.3016709293410074
1107	24	1.377505081872762
1107	25	1.3920018202722204
1108	1	0.4732400439825746
1108	2	0.4956897714920778
1108	3	0.3268709058505159
1108	4	0.5268563390992007
1108	5	0.5074504900670576
1108	6	0.7145852000373125
1108	7	0.7125054225020051
1108	8	0.7750098320465462
1108	9	0.8250340995947596
1108	10	0.824192039912532
1108	11	0.7085047721005324
1108	12	0.770014122641264
1108	13	0.7176816592583393
1108	14	0.9836999259066346
1108	15	0.8574840535728612
1108	16	0.8726329026513469
1108	17	1.050610797421193
1108	18	1.0525612893048972
1108	19	1.1982045061549371
1108	20	1.0237979390434617
1108	21	0.8573897737818044
1108	22	0.8280409610075523
1108	23	0.9870435073653283
1108	24	1.028530266633041
1108	25	1.1005448257187211
1109	1	-0.07819324618838408
1109	2	-0.02300267935219935
1109	3	0.3023738397166298
1109	4	0.3487284614587871
1109	5	0.23272116724359648
1109	6	0.23091436966583245
1109	7	0.4191926424327761
1109	8	0.44838708879357725
1109	9	0.6685749090772575
1109	10	0.7222658824424002
1109	11	0.21235792470384496
1109	12	0.4877625856756822
1109	13	0.4089192557576098
1109	14	0.19165328326985787
1109	15	0.5152096737308511
1109	16	0.4558140118302063
1109	17	0.2723734196883427
1109	18	-1.5284559219433973
1109	19	0.048504671992738224
1109	20	0.32381913386655015
1109	21	0.3349830665553597
1109	22	0.5819518246985425
1109	23	0.08285715967554808
1109	24	0.49830689696396036
1109	25	0.5390162348179572
1110	1	0.23044650668204625
1110	2	0.13321038916100392
1110	3	0.3345270993336875
1110	4	0.0908842112369932
1110	5	0.38103190441864143
1110	6	0.15510332534503846
1110	7	0.30998772196751645
1110	8	0.2993234806063647
1110	9	0.6023258506512477
1110	10	0.7790478644670458
1110	11	0.5581318417506154
1110	12	-0.11911025908790407
1110	13	0.2486425470325173
1110	14	0.5092121024094354
1110	15	0.5107974497762768
1110	16	0.7037625402187189
1110	17	0.11396724315707019
1110	18	0.7683127688703948
1110	19	0.6033433384697133
1110	20	0.676256415480097
1110	21	0.6385237146998648
1110	22	0.6888122477697394
1110	23	0.6156524655179965
1110	24	0.8289492517907675
1110	25	0.97466847168333
1111	1	0.13786207177750853
1111	2	0.4352022267600155
1111	3	0.4055215319409413
1111	4	0.2880659827490213
1111	5	0.41173916194224347
1111	6	0.4707547268097656
1111	7	0.4102303209972149
1111	8	0.2115602339465319
1111	9	0.6248997510753966
1111	10	0.49723278660951564
1111	11	0.3929215625456024
1111	12	0.5226933177785895
1111	13	0.46119892884404834
1111	14	0.3637411761668261
1111	15	0.6628428825763493
1111	16	0.5680978601604565
1111	17	0.5704660559031609
1111	18	0.8573230571091202
1111	19	0.5016136729552514
1111	20	0.4432033054770983
1111	21	0.6514562100020882
1111	22	0.3853868660344588
1111	23	0.7581343968685417
1111	24	0.8186611101964729
1111	25	0.3401252839563611
1112	1	-0.2898231723230147
1112	2	-0.02412736131417148
1112	3	0.005454840507873371
1112	4	0.2470561855304929
1112	5	0.23229632687460366
1112	6	0.120688232980946
1112	7	-0.2868748755992456
1112	8	0.1935488045169329
1112	9	0.3157856504316001
1112	10	0.32523459271232724
1112	11	0.23439513692976988
1112	12	0.27420034656162073
1112	13	-0.06106395594081704
1112	14	0.15831318513015447
1112	15	0.15609301793525532
1112	16	0.3887133183454239
1112	17	0.4720707011837817
1112	18	0.1609004843808819
1112	19	0.13548047188502466
1112	20	0.09274545643883414
1112	21	0.051562619888620986
1112	22	0.0790551319781585
1112	23	0.39229355128045285
1112	24	0.01837369482048956
1112	25	0.32705109541819866
1113	1	-0.34822810111319313
1113	2	-0.10863931245786773
1113	3	-0.36674327635242676
1113	4	-0.11885717072273394
1113	5	-0.3210095680257581
1113	6	-0.13802679996153344
1113	7	-0.3875476119090493
1113	8	-0.0688351060596375
1113	9	-0.2847531852346279
1113	10	-0.5557602608286132
1113	11	-0.42454366103003593
1113	12	-0.15712732068076463
1113	13	-0.12950851153091492
1113	14	-0.21418905661007415
1113	15	-0.5485890639577917
1113	16	-0.49815748496494905
1113	17	-0.449456997065127
1113	18	-0.5873651371011874
1113	19	-0.4683163855496417
1113	20	-0.6380941892142928
1113	21	-0.44772327580638205
1113	22	-0.451372645033544
1113	23	-0.7619295094681746
1113	24	-0.1254413419393714
1113	25	-0.26307260735236326
1114	1	-1.5888100500997453
1114	2	-1.4379169184910552
1114	3	-1.2360080860961808
1114	4	-1.4791643777314327
1114	5	-1.5053886087683495
1114	6	-1.8676263345400343
1114	7	-2.16578925684664
1114	8	-1.6154388959947568
1114	9	-1.6919444637448429
1114	10	-1.8023186813734426
1114	11	-2.5793975753957596
1114	12	-2.128676390167982
1114	13	-2.0201939975887804
1114	14	-2.368164902162821
1114	15	-2.402025019769969
1114	16	-3.060180063191126
1114	17	-4.801365463369603
1114	18	-3.671764010691497
1114	19	-2.787271504675811
1114	20	-1.9606508414135853
1114	21	-3.0145770837031343
1114	22	-2.818416315923546
1114	23	-2.7736462996048274
1114	24	-2.3522342583906486
1114	25	-2.1980149630103583
1115	1	-1.6231087339986345
1115	2	-1.1140845114130045
1115	3	-1.163474388261946
1115	4	-1.2287121018002907
1115	5	-1.0809213111585514
1115	6	-1.1898030184266872
1115	7	-2.3336954945838464
1115	8	-1.6146702454026034
1115	9	-1.5868477728315256
1115	10	-1.1859137812368417
1115	11	-2.0604603233281793
1115	12	-1.6364234180377935
1115	13	-1.5310562671137724
1115	14	-1.5807625566205132
1115	15	-2.892372533738083
1115	16	-1.5836638900660205
1115	17	-1.5908607321700785
1115	18	-1.6203175142786783
1115	19	-1.690091171993
1115	20	-1.4233959771531701
1115	21	-2.25359748462499
1115	22	-2.345482517758345
1115	23	-1.68499999861017
1115	24	-1.5346439146347244
1115	25	-1.9269017611851744
1116	1	-1.7261518355525212
1116	2	-2.2399540174898043
1116	3	-1.9731168605380738
1116	4	-1.6164519718313528
1116	5	-2.313194902663053
1116	6	-2.027733446877644
1116	7	-1.647753571781756
1116	8	-2.38156542796305
1116	9	-2.3339843679430317
1116	10	-3.4610294513794386
1116	11	-1.8828830829278864
1116	12	-1.8436203109725717
1116	13	-2.588948538623441
1116	14	-2.4218005280068304
1116	15	-1.9012623266557185
1116	16	-1.7705386163221455
1116	17	-2.276022092081856
1116	18	-2.126927218753469
1116	19	-2.487714811193676
1116	20	-2.861167678159241
1116	21	-3.365424652990736
1116	22	-2.672414539769491
1116	23	-2.5101691226649834
1116	24	-3.253344838738716
1116	25	-2.7258716796566453
1201	1	0.42403289865749727
1201	2	0.33311537760564036
1201	3	0.5501111813295161
1201	4	0.6858179819800121
1201	5	0.7912824166291372
1201	6	0.7091525045110103
1201	7	0.6063794399579479
1201	8	0.13287681436581522
1201	9	0.4457795515044083
1201	10	0.5664159201268362
1201	11	0.28629789705831854
1201	12	-0.5066709223954007
1201	13	0.08247073635860147
1201	14	0.7328250485141865
1201	15	0.3532146518977086
1201	16	0.8321969301074574
1201	17	0.981117072443439
1201	18	1.196590128256915
1201	19	0.7335427683009783
1201	20	0.6830233297089521
1201	21	0.7796211428967013
1201	22	0.9974363804463522
1201	23	1.155673275016845
1201	24	0.9799606656030733
1201	25	0.9426737926485274
1202	1	0.8116534346434605
1202	2	0.8381163109328789
1202	3	0.617129814475355
1202	4	0.9584485038503026
1202	5	0.9875733270463201
1202	6	0.961534435541509
1202	7	0.8980522963634527
1202	8	1.0298263198253146
1202	9	1.3265409006509685
1202	10	1.5392626542703667
1202	11	1.1808327384408344
1202	12	1.3013389514076863
1202	13	1.2729391600846611
1202	14	1.4162796870686165
1202	15	1.057822624745775
1202	16	1.2648217680046017
1202	17	1.4357911773149965
1202	18	1.7047446011893044
1202	19	1.473281342418737
1202	20	1.3253714089296906
1202	21	1.3701713019106663
1202	22	1.555240891521187
1202	23	1.6806674916663766
1202	24	1.789342868538128
1202	25	1.6350372497418064
1203	1	0.46185490253136763
1203	2	0.5700117917612992
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2211	16	0.26187689584604357
2211	17	0.31531559999937997
2211	18	0.3636426939861934
2211	19	0.1717513947648044
2211	20	-0.011540805158652745
2211	21	0.11839314796232969
2211	22	-0.07751792975827954
2211	23	0.6037376156287735
2211	24	0.28945734048974003
2211	25	0.330077671234811
2212	1	-0.09169094441325853
2212	2	-0.13205362521735609
2212	3	0.024830167195769803
2212	4	-0.16389070854548748
2212	5	-0.08624480874433615
2212	6	-0.24619302110145114
2212	7	-0.3133273846774287
2212	8	-0.09504430949704457
2212	9	0.07427473857612199
2212	10	-0.012819068916918752
2212	11	-0.24365143638762987
2212	12	-0.03904441549445181
2212	13	-0.18344306510849862
2212	14	-0.20603774886142645
2212	15	-0.12429298612200768
2212	16	0.032683379342515195
2212	17	-0.07890249294693064
2212	18	-2.879238088853622
2212	19	-1.1137872866963576
2212	20	-0.3442625928439256
2212	21	-0.4868104954915964
2212	22	-0.15460481249736802
2212	23	-0.23698188662524444
2212	24	-0.6928592454511744
2212	25	-0.22690287392130415
2213	1	-0.026309987598281737
2213	2	0.07017647089425338
2213	3	-0.3175026930699829
2213	4	-0.17207959194744404
2213	5	-0.2352858883646931
2213	6	-0.22317395435433696
2213	7	0.11733246820172383
2213	8	-0.4311536837207903
2213	9	-0.31053003864729334
2213	10	-0.34963818864991225
2213	11	-0.27879854137836446
2213	12	-0.2859173696068993
2213	13	-0.20461041293290094
2213	14	-0.16334500937733054
2213	15	-0.43007337610150387
2213	16	-0.7798504034770914
2213	17	-0.4979150626302271
2213	18	-0.31021995056048723
2213	19	-0.41305744806688693
2213	20	-1.098864575614968
2213	21	-0.6912709183388657
2213	22	-0.5683957654426663
2213	23	-1.0328141641328585
2213	24	-0.7023684515307664
2213	25	-0.8061244927733568
2214	1	-1.217769571187148
2214	2	-3.339851838101069
2214	3	-1.4617140108116473
2214	4	-1.0494128466446817
2214	5	-1.1659764851508143
2214	6	-0.9777467396930497
2214	7	-1.1156924452813826
2214	8	-0.9079024879915565
2214	9	-0.9778766601064532
2214	10	-1.2547623271412789
2214	11	-0.8519115534117461
2214	12	-1.5382347974146313
2214	13	-1.3632617309880075
2214	14	-2.611753886293002
2214	15	-2.7731257943471164
2214	16	-1.8631841836064353
2214	17	-2.3149565292869383
2214	18	-1.7092960520192015
2214	19	-3.897482035980051
2214	20	-2.1992524220485627
2214	21	-1.8783084586897019
2214	22	-2.102010944574186
2214	23	-2.2200976141349393
2214	24	-2.398513825480471
2214	25	-2.152212683707301
2215	1	-0.8162212585341351
2215	2	-2.523856755862795
2215	3	-1.2074780862599965
2215	4	-0.7987101187626067
2215	5	-0.7155594710464754
2215	6	-1.4497140755143008
2215	7	-1.7665811449192184
2215	8	-1.8906778764253929
2215	9	-1.3002331853859914
2215	10	-3.055232056948853
2215	11	-1.9393738390152926
2215	12	-2.321860393244311
2215	13	-1.4745172003533398
2215	14	-3.0216331468863693
2215	15	-2.6471281518858163
2215	16	-2.3618698468908796
2215	17	-2.6845913952574847
2215	18	-1.5542883269018155
2215	19	-2.0471710755586123
2215	20	-2.3371035415238595
2215	21	-2.120834029806119
2215	22	-1.563765390739274
2215	23	-2.311496687120851
2215	24	-2.0126326553874314
2215	25	-1.9791731655981692
2216	1	-2.060170568816009
2216	2	-1.9946259964298534
2216	3	-2.6577914208104403
2216	4	-3.498097885128818
2216	5	-2.55780157505826
2216	6	-2.4846330458409263
2216	7	-2.4904747088237578
2216	8	-3.0101287130456242
2216	9	-3.317961212694499
2216	10	-2.7793373764184786
2216	11	-3.4017471571772333
2216	12	-3.8907313698885773
2216	13	-4.3590679601259374
2216	14	-3.7066114512284756
2216	15	-3.4716613343543656
2216	16	-2.6273948733205224
2216	17	-2.7667997882078836
2216	18	-2.8221564631556397
2216	19	-3.235323590514181
2216	20	-2.9999594925451696
2216	21	-3.4407712142956335
2216	22	-4.22509871841164
2216	23	-4.0655651536921305
2216	24	-3.952361926693545
2216	25	-3.927544080679411
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	125981.0
3	2.0
4	1065.0
5	5828.0
6	7000.0
7	6246.0
8	5538.0
9	5248.0
10	4897.0
11	4552.0
12	4406.0
13	4378.0
14	4338.0
15	4307.0
16	4540.0
17	4747.0
18	5468.0
19	6664.0
20	8410.0
21	10577.0
22	13641.0
23	16396.0
24	18504.0
25	20790.0
26	23378.0
27	25775.0
28	29440.0
29	34243.0
30	41407.0
31	51600.0
32	64302.0
33	81239.0
34	103269.0
35	133453.0
36	179465.0
37	274528.0
38	665247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.120024355050084	18.64114268117203	12.494501807319255	25.744331156458628
2	14.78000785989782	20.373085149893054	41.01486680673151	23.832040183477616
3	18.470602587460277	24.88285632575841	29.821639631459902	26.824901455321417
4	12.242489935583736	17.009963422121515	38.49406734409404	32.2534792982007
5	13.095636904363095	37.85916214083786	36.071913928086076	12.973287026712974
6	31.33711572106973	37.38710322419395	18.400749812546863	12.875031242189452
7	27.437835665412315	31.57544298836693	22.854240731335704	18.13248061488505
8	24.881597614855565	36.7633781947648	20.99908917396777	17.355935016411866
9	26.615244646177842	14.972905960688648	20.222051148747276	38.18979824438624
10	14.856657252118275	28.798770276687748	34.889849783798645	21.454722687395336
11	34.175450418250875	22.30126460028973	25.038715824349627	18.484569157109764
12	23.102771184796975	24.71726276766558	32.028339708584696	20.151626338952752
13	28.962054575665903	22.01322501114748	25.72073711341237	23.30398329977425
14	21.049279460242584	22.02278051458127	27.669616548668053	29.258323476508096
15	23.02611154410107	30.76403924805392	23.392955967170973	22.816893240674037
16	22.398068012479335	27.949282828116196	27.601007184966008	22.051641974438457
17	21.833139705779434	28.341475022045547	27.83372758702742	21.9916576851476
18	21.855040794508636	27.45376130422363	29.404963978066167	21.286233923201568
19	23.54612928527615	26.902913315406625	27.3341341863768	22.216823212940426
20	23.644973715309508	27.414262319011563	27.77473950262047	21.16602446305846
21	23.211057698075933	26.7769943569706	26.873227804817574	23.138720140135895
22	23.339401955756042	27.90086650330375	27.001370688853633	21.758360852086575
23	22.729529081773695	27.668714079256713	27.279893981786742	22.321862857182847
24	23.181878394489335	26.931719058128404	27.923678507961817	21.96272403942044
25	22.96068565772602	27.20715451543805	27.69070417888288	22.14145564795305
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	211.0
1	211.0
2	368.5
3	526.0
4	526.0
5	526.0
6	965.0
7	1404.0
8	1404.0
9	1404.0
10	2117.5
11	2831.0
12	2831.0
13	2831.0
14	5364.5
15	7898.0
16	7898.0
17	7898.0
18	14884.5
19	21871.0
20	21871.0
21	21871.0
22	35519.5
23	49168.0
24	49168.0
25	49168.0
26	71428.5
27	93689.0
28	93689.0
29	93689.0
30	122139.5
31	150590.0
32	150590.0
33	150590.0
34	171444.5
35	192299.0
36	192299.0
37	192299.0
38	208773.0
39	225247.0
40	225247.0
41	225247.0
42	236909.5
43	248572.0
44	248572.0
45	248572.0
46	253822.5
47	259073.0
48	259073.0
49	259073.0
50	250237.0
51	241401.0
52	241401.0
53	241401.0
54	216875.5
55	192350.0
56	192350.0
57	192350.0
58	166057.5
59	139765.0
60	139765.0
61	139765.0
62	114014.0
63	88263.0
64	88263.0
65	88263.0
66	68556.0
67	48849.0
68	48849.0
69	48849.0
70	35800.0
71	22751.0
72	22751.0
73	22751.0
74	15902.0
75	9053.0
76	9053.0
77	9053.0
78	6230.0
79	3407.0
80	3407.0
81	3407.0
82	2271.5
83	1136.0
84	1136.0
85	1136.0
86	763.5
87	391.0
88	391.0
89	391.0
90	244.0
91	97.0
92	97.0
93	97.0
94	55.5
95	14.0
96	14.0
97	14.0
98	13.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3514472961498229
2	0.042131693779053
3	0.03803347445534915
4	0.03763364817986585
5	0.04333117260550291
6	0.01844198695666733
7	0.01824207381892568
8	0.02433942452004604
9	0.021440684022792096
10	0.020940901178437967
11	0.019741422351988062
12	0.021240770885050445
13	0.020840944609567143
14	0.028137774137137413
15	0.02828770899044365
16	0.022090401720452464
17	0.023239902262466958
18	0.09735769808018416
19	0.025089098786577235
20	0.023239902262466958
21	0.026238599328591728
22	0.02074098804069632
23	0.022890054271419068
24	0.01919166122319852
25	0.022690141133677417
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2000869.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.70626114780931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.82590863474267	60.21962143002194
2	11.76055825304828	17.101324391803047
3	2.9818584381416398	6.503993349279745
4	1.0427890521744616	3.032691725978918
5	0.4896998022386658	1.7802120852797507
6	0.24532571070725626	1.0702029113372213
7	0.14959729264000937	0.7613661877982841
8	0.10247423880727002	0.596041501411548
9	0.06387672543565535	0.4179814092712505
>10	0.30470488672955165	3.7538232850463253
>50	0.018243748860138704	0.8943048285543101
>100	0.012206531631790977	1.7983137339967763
>500	0.0019296793898645008	0.9306384628644928
>1k	8.270054527989748E-4	1.1394846973563513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	2957	0.1477857870755157	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2646	0.1322425406161023	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2637	0.13179273605618358	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2342	0.1170491421477368	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2244	0.11215127027306634	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2116	0.1057540498653335	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.995656887082563E-5	0.0	0.0	0.0
3	0.0	9.995656887082563E-5	0.0	0.0	0.0
4	0.0	9.995656887082563E-5	0.0	0.0	0.0
5	0.0	9.995656887082563E-5	0.0	0.0	0.0
6	0.0	9.995656887082563E-5	0.0	0.0	0.0
7	0.0	9.995656887082563E-5	0.0	0.0	0.0
8	0.0	9.995656887082563E-5	0.0	0.0	0.0
9	0.0	9.995656887082563E-5	0.0	0.0	0.0
10	4.997828443541281E-5	9.995656887082563E-5	0.0	0.0	0.0
11	4.997828443541281E-5	9.995656887082563E-5	0.0	0.0	0.0
12	4.997828443541281E-5	9.995656887082563E-5	0.0	0.0	0.0
13	4.997828443541281E-5	9.995656887082563E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTGC	130	0.0	14.607655	8
GCGTGCG	100	1.0146323E-8	13.29363	9
ACCGCCC	50	0.0015058009	13.292965	8
CGTGCGC	80	2.0110056E-6	13.056245	10
CGAAATC	115	5.305992E-9	12.394666	13
TAGGACC	690	0.0	11.700511	4
GCGCCAC	125	1.825174E-8	11.403092	13
GGGCCGC	85	5.35453E-5	11.1702795	6
TGTAGGA	1780	0.0	11.045775	2
GTATAGA	280	0.0	10.887052	1
AAAAGCG	70	0.0014942675	10.854928	5
GCCGGTT	115	8.8739944E-7	10.733987	11
GTAGGAC	1740	0.0	10.644646	3
AGGACGT	1475	0.0	10.560557	5
GGACGTG	1460	0.0	10.470225	6
GTATCAA	1710	0.0	10.417508	1
TGGCGAG	1580	0.0	10.403939	18
CGGTTTC	120	1.5187525E-6	10.294458	13
GGCGAGG	725	0.0	10.215029	19
TAGGACA	625	0.0	10.181922	4
>>END_MODULE