FastQCFastQC Report
Thu 26 May 2016
SRR1512942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000869
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31340.15663194342058376No Hit
GTCCTACAGTGGACATTTCTAAATT27640.138139978179481No Hit
CTTTAGGACGTGAAATATGGCGAGG26210.130993083505217No Hit
GTCCTAAAGTGTGTATTTCTCATTT23220.11604957645902855No Hit
CTGTAGGACGTGGAATATGGCAAGA22790.11390051022830582No Hit
TATCAACGCAGAGTACTTTTTTTTT22470.1123012051263726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCA400.00527067714.25371559
TAGGACC8100.012.5686964
GTGCGTT550.00308660112.08166611
CAGTGCG907.441806E-611.6141399
GGCGAGG7650.011.53730819
TGGCGAG15800.011.53259718
GGTATCA10100.011.4842741
GTATTAG1950.011.21394351
GATATAC4950.010.755931
GTCCTAC16650.010.6780631
GGACCTG12450.010.5426286
ATGGCGA18400.010.26583317
CCCCTAT1303.8010148E-710.2388181
CTAGGAC3350.010.2157083
CCTACAG17900.010.1435773
TATGGCG18200.010.11787416
ACTGTTC5550.010.1040098
AGGACCT13900.09.9937745
GAATAAC1151.01705955E-59.9208411
GACCTGG13250.09.8993977
Produced by FastQC (version 0.11.5)