##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512942_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2000869 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.53688422380476 31.0 31.0 34.0 27.0 34.0 2 30.657988104168737 31.0 31.0 34.0 27.0 34.0 3 30.768949891272243 31.0 31.0 34.0 27.0 34.0 4 34.26899762053388 37.0 35.0 37.0 30.0 37.0 5 33.94210615487571 37.0 35.0 37.0 30.0 37.0 6 33.82416190165373 37.0 35.0 37.0 29.0 37.0 7 33.69409991358754 37.0 35.0 37.0 28.0 37.0 8 33.57168560260567 37.0 35.0 37.0 28.0 37.0 9 35.0413885166895 39.0 35.0 39.0 28.0 39.0 10 34.92544389462778 39.0 35.0 39.0 28.0 39.0 11 34.97682856798721 39.0 35.0 39.0 27.0 39.0 12 34.828760903387476 39.0 35.0 39.0 27.0 39.0 13 34.825316899807035 39.0 35.0 39.0 27.0 39.0 14 35.886528803234995 40.0 36.0 41.0 27.0 41.0 15 35.89961411766588 40.0 36.0 41.0 27.0 41.0 16 35.87877367283915 40.0 36.0 41.0 27.0 41.0 17 35.85036101813762 40.0 36.0 41.0 27.0 41.0 18 35.771058974875416 40.0 36.0 41.0 27.0 41.0 19 35.75271244644202 40.0 36.0 41.0 27.0 41.0 20 35.73422647859505 40.0 36.0 41.0 27.0 41.0 21 35.671620680814186 40.0 36.0 41.0 27.0 41.0 22 35.63998042850382 40.0 36.0 41.0 27.0 41.0 23 35.53560228080899 40.0 36.0 41.0 27.0 41.0 24 35.521577874413566 40.0 36.0 41.0 27.0 41.0 25 35.42386283159967 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7019543699884352 1101 2 0.6320046472563767 1101 3 0.6454181097913931 1101 4 0.43599282197715183 1101 5 0.6244034296813297 1101 6 0.7555441683228281 1101 7 0.7355026736064048 1101 8 0.661760425247607 1101 9 0.5951112721717067 1101 10 0.814294940660325 1101 11 0.8227095461996683 1101 12 0.9050223348965858 1101 13 0.9974190674604699 1101 14 1.0711203120726438 1101 15 0.9727631907868428 1101 16 1.1285683657737522 1101 17 1.0338209194453327 1101 18 0.3878294418716166 1101 19 0.8445952449084544 1101 20 0.9225239193104571 1101 21 1.0109871771314545 1101 22 1.0039107005846617 1101 23 1.0234679884352431 1101 24 0.8931392033192367 1101 25 0.9333866420075054 1102 1 0.6733397330029156 1102 2 0.6844761003309969 1102 3 0.6541921139676958 1102 4 0.5505068358100758 1102 5 0.7676020560206922 1102 6 0.7351578293556429 1102 7 0.7869064854611665 1102 8 0.8025050718115452 1102 9 1.0408509154858976 1102 10 1.0149930699676801 1102 11 0.9160168609878454 1102 12 0.8959664734382358 1102 13 0.9993821895377053 1102 14 1.1961725364503906 1102 15 1.1455493196628126 1102 16 1.2367804665171036 1102 17 1.0873832703100064 1102 18 1.2241905542518907 1102 19 1.2517956642676396 1102 20 1.2383460843448404 1102 21 1.2706373159916993 1102 22 1.289542338740418 1102 23 1.2860830312787854 1102 24 1.2287726731871587 1102 25 1.2856529844817004 1103 1 0.5144700926424512 1103 2 0.4647335772664718 1103 3 0.5491293095483272 1103 4 0.451729769315655 1103 5 0.5346262584849057 1103 6 0.6488820918543681 1103 7 0.6987463755604253 1103 8 0.7130252617156714 1103 9 0.796243629965403 1103 10 0.9251298503428629 1103 11 0.8031836434334636 1103 12 0.8549912162930511 1103 13 0.9542364919182234 1103 14 1.013907820199421 1103 15 0.8155595199350856 1103 16 0.8921347700967104 1103 17 0.9686976865082926 1103 18 1.037672460359822 1103 19 1.1873813645411104 1103 20 1.198235971766131 1103 21 0.8080730546760719 1103 22 1.1353598473958613 1103 23 1.0599919179062738 1103 24 1.1357303620420822 1103 25 1.2096855374195954 1104 1 0.5791442534193507 1104 2 0.6094629896249693 1104 3 0.47396397277865887 1104 4 0.48697788882184057 1104 5 0.5314963738933614 1104 6 0.6545754258606706 1104 7 0.6316422413919796 1104 8 0.6165053384969141 1104 9 0.7254153767387166 1104 10 0.7975423476418015 1104 11 0.8359491483395445 1104 12 0.6694554037099536 1104 13 0.7246989380349191 1104 14 0.866648080984362 1104 15 0.899750359497304 1104 16 0.8959091644665023 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-2.424001847739568 2216 19 -2.500545237974606 2216 20 -2.6229366239636747 2216 21 -2.4755138232490594 2216 22 -2.876697260725777 2216 23 -2.0017686046246936 2216 24 -2.3991616514026006 2216 25 -2.2879644240421584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46883.0 3 0.0 4 744.0 5 6465.0 6 7542.0 7 5954.0 8 4758.0 9 4074.0 10 3604.0 11 3436.0 12 3074.0 13 2967.0 14 2929.0 15 2994.0 16 3101.0 17 3265.0 18 3673.0 19 4257.0 20 5559.0 21 7093.0 22 9337.0 23 11364.0 24 13566.0 25 15712.0 26 18054.0 27 20261.0 28 23227.0 29 27863.0 30 35135.0 31 45428.0 32 57656.0 33 74414.0 34 96581.0 35 125168.0 36 171738.0 37 265332.0 38 867661.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.45202286580867 19.556928691148027 13.476552242590845 27.514496200452456 2 13.760605418459226 20.78706998576615 39.90171246021718 25.550612135557436 3 16.838933483401462 25.05096535555301 30.33731843513993 27.772782725905593 4 11.581646503363828 17.695279242455072 37.79675650705837 32.92631774712274 5 12.734811672998001 38.11870565668741 36.12647331711111 13.020009353203472 6 31.58018988247133 37.7757282145826 18.006974294542903 12.637107608403165 7 27.35095553152331 31.659009515067066 22.766964103133308 18.22307085027631 8 24.820448849117014 37.18838328187342 20.618359208339356 17.37280866067021 9 26.18948675981031 14.928487023348922 20.039785828623895 38.84224038821687 10 14.481610522254861 29.16136896193008 34.89915221713009 21.457868298684968 11 34.063954601319594 22.22971936206337 25.000412342691263 18.70591369392577 12 22.892223929010065 24.781443122725268 32.08603410432045 20.240298843944217 13 28.81930733896806 21.865106538244305 25.905712529177432 23.409873593610204 14 20.773700481594688 21.97101434787281 27.711263117644556 29.544022052887943 15 22.79059561410568 30.868006803819377 23.353665150319323 22.98773243175562 16 22.26936335968838 27.961343267465715 27.51932636664307 22.249967006202834 17 21.648291323024743 28.484949083665978 27.763201049524433 22.10355854378485 18 21.62255771448106 27.609040398512423 29.38434388339703 21.384058003609482 19 23.434526900621915 26.95690072687633 27.39248672874663 22.216085643755125 20 23.58308696184595 27.389745114847013 27.729275430410784 21.297892492896253 21 23.03118901075285 26.668615823656268 27.160003298573365 23.140191867017514 22 23.200635327127 27.779868546459298 27.127998524633668 21.891497601780035 23 22.415810330411436 27.576118176652244 27.48910598345019 22.518965509486126 24 23.084119949881774 26.958336602746108 27.91577059767531 22.041772849696805 25 22.760410002559016 27.22409594715629 27.67432305994498 22.34117099033971 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 138.0 1 138.0 2 248.0 3 358.0 4 358.0 5 358.0 6 843.0 7 1328.0 8 1328.0 9 1328.0 10 1974.0 11 2620.0 12 2620.0 13 2620.0 14 5120.0 15 7620.0 16 7620.0 17 7620.0 18 14713.0 19 21806.0 20 21806.0 21 21806.0 22 36136.5 23 50467.0 24 50467.0 25 50467.0 26 72869.0 27 95271.0 28 95271.0 29 95271.0 30 123088.0 31 150905.0 32 150905.0 33 150905.0 34 171959.5 35 193014.0 36 193014.0 37 193014.0 38 209906.0 39 226798.0 40 226798.0 41 226798.0 42 238366.5 43 249935.0 44 249935.0 45 249935.0 46 255346.5 47 260758.0 48 260758.0 49 260758.0 50 251780.5 51 242803.0 52 242803.0 53 242803.0 54 217080.0 55 191357.0 56 191357.0 57 191357.0 58 164334.5 59 137312.0 60 137312.0 61 137312.0 62 112064.5 63 86817.0 64 86817.0 65 86817.0 66 66962.0 67 47107.0 68 47107.0 69 47107.0 70 34348.5 71 21590.0 72 21590.0 73 21590.0 74 14983.5 75 8377.0 76 8377.0 77 8377.0 78 5768.5 79 3160.0 80 3160.0 81 3160.0 82 2067.5 83 975.0 84 975.0 85 975.0 86 630.5 87 286.0 88 286.0 89 286.0 90 174.5 91 63.0 92 63.0 93 63.0 94 33.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07756629744376069 2 2.4989142217706406E-4 3 0.0 4 0.03088657978108512 5 0.07766625401263151 6 0.07631684033287536 7 0.03888310529075117 8 0.07886573283908142 9 0.058074766513949695 10 0.11804870783644505 11 0.0052976981501537575 12 0.017992182396748612 13 0.008946112913938894 14 0.011195135713532471 15 0.01139504885127412 16 0.024639294226658517 17 0.03623425621567429 18 0.0013993919641915589 19 0.0057974809945078865 20 0.0081964386474077 21 1.9991313774165125E-4 22 4.997828443541281E-4 23 0.0 24 0.0 25 0.005047806727976694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2000869.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.67046114018305 #Duplication Level Percentage of deduplicated Percentage of total 1 81.05006553592736 57.27845506866044 2 12.856791699772687 18.171907964124273 3 3.3856221184174133 7.17790429064886 4 1.187634404222295 3.3572268404934458 5 0.5330872680040782 1.8836761528904278 6 0.2615075295708599 1.1088514623841634 7 0.16841781212202017 0.8331515112818714 8 0.11215160014006202 0.6340644239606081 9 0.07272697802700452 0.46256841670503324 >10 0.3360224120671784 4.040113775062255 >50 0.02034694487510638 0.988022439160213 >100 0.012657606697061483 1.804083346944842 >500 0.0020493955846186676 0.9875526376764676 >1k 9.186945724152312E-4 1.2724216700070965 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3134 0.15663194342058376 No Hit GTCCTACAGTGGACATTTCTAAATT 2764 0.138139978179481 No Hit CTTTAGGACGTGAAATATGGCGAGG 2621 0.130993083505217 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2322 0.11604957645902855 No Hit CTGTAGGACGTGGAATATGGCAAGA 2279 0.11390051022830582 No Hit TATCAACGCAGAGTACTTTTTTTTT 2247 0.1123012051263726 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.997828443541281E-5 0.0 0.0 0.0 6 0.0 4.997828443541281E-5 0.0 0.0 0.0 7 0.0 4.997828443541281E-5 0.0 0.0 0.0 8 0.0 4.997828443541281E-5 0.0 0.0 0.0 9 0.0 4.997828443541281E-5 0.0 4.997828443541281E-5 0.0 10 0.0 4.997828443541281E-5 0.0 4.997828443541281E-5 0.0 11 0.0 4.997828443541281E-5 0.0 4.997828443541281E-5 0.0 12 0.0 4.997828443541281E-5 0.0 4.997828443541281E-5 0.0 13 0.0 9.995656887082563E-5 0.0 9.995656887082563E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGCA 40 0.005270677 14.2537155 9 TAGGACC 810 0.0 12.568696 4 GTGCGTT 55 0.003086601 12.081666 11 CAGTGCG 90 7.441806E-6 11.614139 9 GGCGAGG 765 0.0 11.537308 19 TGGCGAG 1580 0.0 11.532597 18 GGTATCA 1010 0.0 11.484274 1 GTATTAG 195 0.0 11.2139435 1 GATATAC 495 0.0 10.75593 1 GTCCTAC 1665 0.0 10.678063 1 GGACCTG 1245 0.0 10.542628 6 ATGGCGA 1840 0.0 10.265833 17 CCCCTAT 130 3.8010148E-7 10.238818 1 CTAGGAC 335 0.0 10.215708 3 CCTACAG 1790 0.0 10.143577 3 TATGGCG 1820 0.0 10.117874 16 ACTGTTC 555 0.0 10.104009 8 AGGACCT 1390 0.0 9.993774 5 GAATAAC 115 1.01705955E-5 9.920841 1 GACCTGG 1325 0.0 9.899397 7 >>END_MODULE