FastQCFastQC Report
Thu 26 May 2016
SRR1512941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192737
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40580.18506551401285243No Hit
TATCAACGCAGAGTACTTTTTTTTT25600.11674906748962598No Hit
GGTATCAACGCAGAGTACTTTTTTT24900.11355671017545652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC1157.133349E-811.55876110
GGTATCA12650.011.1501311
AAGGCGT1451.2296368E-911.1312066
AGGCGTG1909.094947E-1210.4936597
ATAAGCC1752.3646862E-1010.3111153
TGGCGAG8400.010.29460918
GTGTTAT2002.3646862E-1110.00686551
GCGTGCG1054.131569E-59.9468259
ATGGCGA9050.09.87022617
TATGGCG9150.09.65849916
GGCGAGA5900.09.65562319
GTATTAC1606.3184416E-89.5303491
GTACTAA1606.3184416E-89.5303491
GTCCTAT1909.931682E-109.5303481
GTATCAA22900.09.4054971
GTGTTAG3000.09.212671
GGCGTGC1554.2958527E-79.18841658
GTCCTAC7900.09.1684371
GTCTAGA2201.5825208E-109.0971511
TAGGACC3550.09.0957954
Produced by FastQC (version 0.11.5)