##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512941_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2192737
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.79899413381541	31.0	30.0	34.0	22.0	34.0
2	28.750857946028184	31.0	30.0	34.0	16.0	34.0
3	29.00591315784793	31.0	30.0	34.0	22.0	34.0
4	32.41041173656485	35.0	33.0	37.0	23.0	37.0
5	32.572975692023256	35.0	33.0	37.0	25.0	37.0
6	32.55032409267505	36.0	33.0	37.0	25.0	37.0
7	32.39629741277682	36.0	33.0	37.0	23.0	37.0
8	32.40232275918179	36.0	33.0	37.0	23.0	37.0
9	33.693746673677694	38.0	34.0	39.0	21.0	39.0
10	33.58006226920967	38.0	34.0	39.0	19.0	39.0
11	33.6414955373125	38.0	34.0	39.0	21.0	39.0
12	33.52968960709834	38.0	34.0	39.0	18.0	39.0
13	33.51766810155527	38.0	34.0	39.0	18.0	39.0
14	34.49397123321219	39.0	34.0	41.0	17.0	41.0
15	34.424471790278545	39.0	34.0	41.0	17.0	41.0
16	34.302007947145505	39.0	34.0	41.0	17.0	41.0
17	34.236145967345834	39.0	34.0	41.0	17.0	41.0
18	33.92331228049693	39.0	33.0	41.0	11.0	41.0
19	34.16425453668178	39.0	34.0	41.0	17.0	41.0
20	34.24747381924964	39.0	34.0	41.0	16.0	41.0
21	34.18632649515195	39.0	34.0	41.0	11.0	41.0
22	34.08675504631882	39.0	34.0	41.0	10.0	41.0
23	33.81390882718721	39.0	34.0	41.0	2.0	41.0
24	33.81869827526055	39.0	34.0	41.0	2.0	41.0
25	33.73711667199486	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7301102242605175
1101	2	0.9120596495388007
1101	3	1.0127709625077088
1101	4	0.8834137007532377
1101	5	0.8266853810711083
1101	6	1.0094376380286434
1101	7	1.0096766720470285
1101	8	0.8230158215296726
1101	9	0.6834408290246472
1101	10	0.8903434712284053
1101	11	1.0770964138447
1101	12	1.2253311278303585
1101	13	1.0762534632613878
1101	14	1.0342979297052253
1101	15	0.8958185931756262
1101	16	1.330758085457532
1101	17	1.2079290953677813
1101	18	1.5460665559761964
1101	19	0.930565030024745
1101	20	1.157546469789743
1101	21	1.1525699392712951
1101	22	1.2177629387342535
1101	23	1.3258349879400697
1101	24	0.9942579386891808
1101	25	1.2588890411201703
1102	1	0.7503093171108084
1102	2	0.790020277023963
1102	3	0.8107752770582408
1102	4	0.8968518003457717
1102	5	0.8577289398818735
1102	6	0.7642095415262986
1102	7	0.9413961014842158
1102	8	0.9123743308197163
1102	9	0.97816687555747
1102	10	1.0957486981246944
1102	11	1.050566556961094
1102	12	1.0847439632797133
1102	13	0.9404292829336001
1102	14	1.042985852238722
1102	15	1.234703374624118
1102	16	1.1190398415977896
1102	17	0.9903613623949497
1102	18	1.300630068622631
1102	19	1.3256288929471083
1102	20	1.2521035898318331
1102	21	1.0366317166385102
1102	22	1.3365458154098633
1102	23	1.4893806402647272
1102	24	1.3543246713310424
1102	25	1.1839127938350629
1103	1	0.4790672023847229
1103	2	0.5752794196835715
1103	3	0.6443922208185668
1103	4	0.7715917848574776
1103	5	0.20337943489411714
1103	6	0.614267998078958
1103	7	0.4775628357921633
1103	8	0.4263536362989768
1103	9	0.7541584858009429
1103	10	0.7307673446783767
1103	11	0.7812568566717388
1103	12	0.8854861856958962
1103	13	0.8395702180864859
1103	14	1.009595472895711
1103	15	0.9885435116334875
1103	16	1.1630114135322174
1103	17	1.0010612879571568
1103	18	1.363348286836512
1103	19	1.082978278318464
1103	20	1.1133980371749104
1103	21	0.9846686383519625
1103	22	1.0479185964770465
1103	23	1.4102891100423278
1103	24	1.3017479626761315
1103	25	1.2614671561321131
1104	1	0.5548465758233867
1104	2	0.44705306160871316
1104	3	0.38024912386791954
1104	4	0.701867489396804
1104	5	0.36357932815805327
1104	6	0.49338721751072256
1104	7	0.6366082279877716
1104	8	0.6371294865384485
1104	9	0.7540029091561067
1104	10	0.6879910217655905
1104	11	0.6758267597929333
1104	12	0.5571079393663254
1104	13	0.6705472062030537
1104	14	0.8036540357905437
1104	15	0.933375858570102
1104	16	0.6992275587869656
1104	17	0.7728674960945838
1104	18	0.9069027551812994
1104	19	0.8843584704049263
1104	20	0.9210211354470843
1104	21	0.9663911794674576
1104	22	0.923473974508191
1104	23	1.1341902101440127
1104	24	0.9774230123601413
1104	25	0.6919856651205123
1105	1	0.6881197256819611
1105	2	0.7320077221064452
1105	3	0.7802562333752121
1105	4	0.8750446471020652
1105	5	0.5993450850781343
1105	6	0.38667161488593393
1105	7	0.7622938769813885
1105	8	0.9057429790373135
1105	9	0.8566245054579227
1105	10	0.903355340011295
1105	11	0.7686632927209089
1105	12	0.8350951964373081
1105	13	0.8191372890961119
1105	14	0.9321905626961424
1105	15	1.162278007418685
1105	16	1.1457216139791697
1105	17	0.9643124637627167
1105	18	1.1184538236177275
1105	19	1.083041416922967
1105	20	1.0410963133098718
1105	21	1.1287690473641874
1105	22	0.8271293656458454
1105	23	1.0848514374623974
1105	24	1.0248723299423546
1105	25	1.0605338951034042
1106	1	0.7118732406367982
1106	2	0.8124223132066746
1106	3	0.8369994759264792
1106	4	0.8994828258659666
1106	5	0.8745274645514414
1106	6	0.9057478451485963
1106	7	0.8025830014983342
1106	8	0.9140268543525778
1106	9	1.0519289906216969
1106	10	1.1716126268175202
1106	11	1.109938169464975
1106	12	1.1048320690674842
1106	13	1.0518635949071609
1106	14	1.2656057249963055
1106	15	1.3140283922889964
1106	16	1.2151339208701941
1106	17	1.0583341153699024
1106	18	1.4015407969270584
1106	19	1.014359159391688
1106	20	1.1243895968473296
1106	21	0.986950168274241
1106	22	0.9855450994833745
1106	23	1.4132530992484291
1106	24	1.319929700062275
1106	25	1.42653411123311
1107	1	0.4494150641494379
1107	2	0.43141079960120976
1107	3	0.5641411568359835
1107	4	0.6424866091431554
1107	5	0.6335925751662401
1107	6	0.5142423185280975
1107	7	0.724503540194668
1107	8	0.5786860423435556
1107	9	0.7837729300633001
1107	10	0.6130145215019098
1107	11	0.8032423130946924
1107	12	1.0528281105322392
1107	13	0.9146595051530682
1107	14	1.0366388138171914
1107	15	1.1383728847171994
1107	16	1.1048786283015843
1107	17	1.1344457931986582
1107	18	1.4041052566903574
1107	19	1.2915647271054596
1107	20	1.1310434112565346
1107	21	1.1528054550508529
1107	22	1.0523301527173885
1107	23	1.2101594700036031
1107	24	1.1649878472900284
1107	25	1.2254172530558236
1108	1	0.25382359067139504
1108	2	0.3425088159808638
1108	3	0.24458275350701086
1108	4	0.38444335221890213
1108	5	0.3028027942153031
1108	6	0.40463594417155235
1108	7	0.3804174813550105
1108	8	0.44515069963029674
1108	9	0.6367230639370831
1108	10	0.5433126878757619
1108	11	0.3854768959497932
1108	12	0.5879410046431488
1108	13	0.5201982458830017
1108	14	0.6459826321945883
1108	15	0.6284410599895196
1108	16	0.5430179264835004
1108	17	0.6844010363324387
1108	18	0.7855711523551037
1108	19	0.763761404681091
1108	20	0.6487942125648161
1108	21	0.6626187327778652
1108	22	0.49832050295846386
1108	23	0.8286927476512602
1108	24	0.837024015202843
1108	25	1.0221374196366781
1109	1	0.005523967458906043
1109	2	-0.002953975977913359
1109	3	0.30850262452357313
1109	4	0.3002534724032486
1109	5	0.30554577078763145
1109	6	0.21535678834320748
1109	7	0.3295003872811577
1109	8	0.35383148032414624
1109	9	0.5371589457612984
1109	10	0.645363515745359
1109	11	0.18668004063926702
1109	12	0.4692471934586919
1109	13	0.42008602245140736
1109	14	0.35878730715135987
1109	15	0.5131778283636379
1109	16	0.5308466438867825
1109	17	0.18641652928328512
1109	18	-1.2241243201281833
1109	19	0.17939341389284635
1109	20	0.43346244032177594
1109	21	0.5221884545208724
1109	22	0.6189534255381588
1109	23	0.09939286741145992
1109	24	0.620627548289157
1109	25	0.7995025905598467
1110	1	0.2897402057495384
1110	2	0.14059655734026322
1110	3	0.3007482974717668
1110	4	0.13721146731913336
1110	5	0.30160719817882864
1110	6	0.3299275744532082
1110	7	0.3039951481836809
1110	8	0.1957960318992562
1110	9	0.5269335070423509
1110	10	0.5898621646183955
1110	11	0.4475442991036189
1110	12	0.031156067465310855
1110	13	0.29879024248867125
1110	14	0.41105520942471685
1110	15	0.5154165263588482
1110	16	0.6088471132911977
1110	17	0.30763678751674206
1110	18	0.7504106173171934
1110	19	0.5198646949117389
1110	20	0.608484175068611
1110	21	0.6506623587120259
1110	22	0.6280714665095815
1110	23	0.58068674534546
1110	24	0.6011701503000708
1110	25	0.6238420996380896
1111	1	0.055715095319936125
1111	2	0.25375144577605013
1111	3	0.23889437044929807
1111	4	0.20078065448382887
1111	5	0.12429913323030917
1111	6	0.21625766303048977
1111	7	0.1545049986935325
1111	8	-0.06711152314312585
1111	9	0.28566470397579735
1111	10	0.2491182845486719
1111	11	0.16259531752542244
1111	12	0.20826501985516188
1111	13	0.3206592531217254
1111	14	0.17030142934509485
1111	15	0.4648630713110151
1111	16	0.3405368225090015
1111	17	0.39933201404343066
1111	18	0.6819759646128745
1111	19	0.4391662658987059
1111	20	0.2099531585600687
1111	21	0.4577812444825966
1111	22	0.21069038620715475
1111	23	0.4485238379016536
1111	24	0.6065083691214355
1111	25	-0.08477057526671672
1112	1	-0.19083590756018154
1112	2	0.11426776831769203
1112	3	0.09487315047871192
1112	4	0.2109196772810833
1112	5	0.11533811477562494
1112	6	0.15775142414450727
1112	7	-0.1691319357028931
1112	8	0.1701726603435887
1112	9	0.30362134535015883
1112	10	0.32088982467464433
1112	11	0.2368111290830015
1112	12	0.23862091097054616
1112	13	0.013083404406636134
1112	14	0.1793125174701018
1112	15	0.2513919717639652
1112	16	0.45446155668690125
1112	17	0.34337445808587574
1112	18	0.2612157463843019
1112	19	0.17592813557196507
1112	20	0.09398992234366688
1112	21	0.2063993728475637
1112	22	0.08735161413420656
1112	23	0.3128445155134685
1112	24	0.044449148834367236
1112	25	0.3661091129547387
1113	1	-0.2460315230401946
1113	2	-0.06123497909729636
1113	3	-0.28633872828336493
1113	4	0.036972891951798204
1113	5	-0.1592006101128618
1113	6	0.0019284191274593354
1113	7	-0.121694944076431
1113	8	0.1423005531868924
1113	9	-0.00382163190079865
1113	10	-0.2146694224979484
1113	11	-0.11109954097663177
1113	12	-2.8791702482777737E-4
1113	13	-0.03228697862995489
1113	14	-0.011106162616286497
1113	15	-0.354140693764073
1113	16	-0.32463086382423967
1113	17	-0.30929166384670026
1113	18	-0.3614646990075485
1113	19	-0.3184932890934107
1113	20	-0.3821563297512185
1113	21	-0.30186422551369674
1113	22	-0.17257054271918548
1113	23	-0.5680850146016638
1113	24	0.007233845521014359
1113	25	0.09101269735621997
1114	1	-1.5746820766245158
1114	2	-1.3414010668549707
1114	3	-1.1230808261963858
1114	4	-1.3979183310346386
1114	5	-1.4634332226968851
1114	6	-1.8614879940847935
1114	7	-1.9758446872435442
1114	8	-1.4192910713112141
1114	9	-1.5741225621151926
1114	10	-1.6395366445074018
1114	11	-2.4464191095405816
1114	12	-1.9301156732533258
1114	13	-1.7415090362671926
1114	14	-2.184429853466419
1114	15	-1.9909008194098945
1114	16	-2.7116823824322687
1114	17	-5.014583343424938
1114	18	-3.8092103600978042
1114	19	-2.792567747783732
1114	20	-1.7825334998291709
1114	21	-2.849962548934549
1114	22	-2.7969888160279197
1114	23	-2.8848360183808417
1114	24	-2.601757174674198
1114	25	-2.403419640907188
1115	1	-1.1958709693610245
1115	2	-0.7813489008328034
1115	3	-0.9385659197886085
1115	4	-0.9734801364484369
1115	5	-0.7319731112738346
1115	6	-0.7953716932772643
1115	7	-1.7742073915129417
1115	8	-1.2825848531246784
1115	9	-1.1373334502020498
1115	10	-0.8965069490488702
1115	11	-1.7703943224154735
1115	12	-1.346322512522967
1115	13	-1.1239690377713458
1115	14	-1.1353451142593443
1115	15	-2.4687774507344677
1115	16	-1.1777939724298108
1115	17	-1.238881618689959
1115	18	-1.3881088239605148
1115	19	-1.4072138213236087
1115	20	-1.129472093181306
1115	21	-1.8415768939294779
1115	22	-1.819048878028795
1115	23	-1.2564590081253257
1115	24	-1.1814287072494452
1115	25	-1.3846655757674284
1116	1	-1.147584577979739
1116	2	-1.8280424360178316
1116	3	-1.473924162369233
1116	4	-1.145165291462117
1116	5	-1.6759028269716474
1116	6	-1.4974726606998345
1116	7	-1.1734130292244949
1116	8	-1.8897340357656418
1116	9	-1.947248868937777
1116	10	-2.7540328886024987
1116	11	-1.4887500938199878
1116	12	-1.4887441871540474
1116	13	-1.9870360245735519
1116	14	-1.7791669401232753
1116	15	-1.4541835577356181
1116	16	-1.3780999461277688
1116	17	-1.6469940922826751
1116	18	-1.4370528444159305
1116	19	-1.8467232499908093
1116	20	-2.2776500490431424
1116	21	-2.7233978466964928
1116	22	-1.909018553501781
1116	23	-1.7543125149347034
1116	24	-2.405324122711061
1116	25	-2.099427539923685
1201	1	0.41676278120838717
1201	2	0.43730771850430017
1201	3	0.6724334600393433
1201	4	0.7374847835446445
1201	5	0.7925390817250388
1201	6	0.8249392780815299
1201	7	0.6275993571382088
1201	8	0.19935260319360992
1201	9	0.5987900362094081
1201	10	0.8698091791249212
1201	11	0.44017267790533054
1201	12	-0.1292149673063534
1201	13	0.19787138511092905
1201	14	0.7777244189336869
1201	15	0.44665020596438865
1201	16	1.0191043099936863
1201	17	1.1962020975377143
1201	18	1.4609837818372569
1201	19	0.9380075863960755
1201	20	0.9365079488091226
1201	21	0.7671874519779749
1201	22	1.0397392853197474
1201	23	1.2259041439443763
1201	24	1.0831976292501864
1201	25	1.060729933291114
1202	1	0.6889324809803163
1202	2	0.7378588517075606
1202	3	0.40216784316902476
1202	4	0.8163518075142662
1202	5	0.8811337239411259
1202	6	0.7300770595860442
1202	7	0.7965883313306108
1202	8	0.9037192131599241
1202	9	1.2062884943064276
1202	10	1.3253728689465802
1202	11	0.9031438789208863
1202	12	0.9804073066554295
1202	13	0.9198737546163613
1202	14	1.1541131981731496
1202	15	0.7885251474160881
1202	16	0.9737600127354469
1202	17	1.2777836970553693
1202	18	1.4547925485043791
1202	19	1.2963649700029478
1202	20	1.1430953750899064
1202	21	1.089529107500816
1202	22	1.2921500833020332
1202	23	1.399155299933767
1202	24	1.531774053344769
1202	25	1.2916956383330458
1203	1	0.28892705733048274
1203	2	0.4697435124748317
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2211	17	-0.19134831550152853
2211	18	-0.2665390555178604
2211	19	-0.4034630902677705
2211	20	-0.42337883459725134
2211	21	-0.3258562439583841
2211	22	-0.4716362803989895
2211	23	0.07778748899936261
2211	24	-0.14102261596413967
2211	25	-0.10525979138912334
2212	1	-0.013173305940906488
2212	2	0.02581060987903072
2212	3	-0.06401134381415474
2212	4	-0.112106461516575
2212	5	-0.00978267395613841
2212	6	-0.1480794248580537
2212	7	-0.220526069044368
2212	8	-0.029275534600884612
2212	9	0.040768395948902025
2212	10	0.078296624537046
2212	11	-0.1753431613758707
2212	12	-0.00887250515206972
2212	13	-0.15763904904512316
2212	14	-0.10701482836897469
2212	15	-0.23785538465045164
2212	16	0.003156043214126214
2212	17	-0.09331151376873237
2212	18	-2.8551308792105985
2212	19	-1.0401210669487284
2212	20	-0.4893097687224639
2212	21	-0.4918273341256949
2212	22	-0.24842986449568372
2212	23	-0.28388291843130986
2212	24	-0.5510740467486741
2212	25	-0.31136921639404136
2213	1	-0.1648345148324779
2213	2	-0.0657476646109103
2213	3	-0.5312473500428894
2213	4	-0.39514003255835206
2213	5	-0.5029983687751312
2213	6	-0.21967744726210015
2213	7	-0.20046057911083182
2213	8	-0.5434480418277765
2213	9	-0.5313051331459064
2213	10	-0.5886104334370543
2213	11	-0.6345877422160484
2213	12	-0.621936596853466
2213	13	-0.3852928444775685
2213	14	-0.45499227480375026
2213	15	-0.5527365382862612
2213	16	-1.3264170716645225
2213	17	-0.692628090767144
2213	18	-0.5371627206473022
2213	19	-0.5011280870708816
2213	20	-1.071366580995651
2213	21	-0.8837925170677963
2213	22	-0.73253111980641
2213	23	-1.1897618076854002
2213	24	-0.961235021928033
2213	25	-1.2091979996357338
2214	1	-1.7402675980446425
2214	2	-3.7276674899010835
2214	3	-1.9697869044372105
2214	4	-1.5565617014140471
2214	5	-1.5475196499336406
2214	6	-1.6520961285854163
2214	7	-1.8927378008814948
2214	8	-1.4717397941571946
2214	9	-1.7350288788604296
2214	10	-1.838348347606491
2214	11	-1.4829246836972558
2214	12	-1.8630885194490254
2214	13	-1.9705861380333864
2214	14	-2.9283029373904803
2214	15	-3.166322621264989
2214	16	-2.4584209683236047
2214	17	-2.828936980099776
2214	18	-2.2264582986212638
2214	19	-4.236892748408881
2214	20	-2.57818213092942
2214	21	-2.47330968149247
2214	22	-2.4426931067791067
2214	23	-2.619800798885219
2214	24	-2.8080982359756916
2214	25	-2.7000688523164236
2215	1	-1.2162975766927353
2215	2	-2.928653761629157
2215	3	-1.556987225432298
2215	4	-1.1764957290425535
2215	5	-1.1370048218236448
2215	6	-1.7002654534926265
2215	7	-2.088060712622177
2215	8	-1.989000445032051
2215	9	-1.7464670156559237
2215	10	-3.0991133261829376
2215	11	-2.1776674039873
2215	12	-2.458629965789825
2215	13	-1.6978178778554032
2215	14	-3.2223570945634314
2215	15	-3.0874300184820775
2215	16	-2.6738608856387067
2215	17	-2.981760823299407
2215	18	-1.6929231790362635
2215	19	-2.286319453337015
2215	20	-2.5872655574086494
2215	21	-2.328941604740251
2215	22	-1.8982270477102787
2215	23	-2.360433814806001
2215	24	-2.21371054484748
2215	25	-2.2152254807216245
2216	1	-1.9787025846201338
2216	2	-1.8607665493389582
2216	3	-2.339382939171294
2216	4	-3.013550496414485
2216	5	-2.3326136595033766
2216	6	-2.2032696632132556
2216	7	-2.159648173668593
2216	8	-2.777790013672366
2216	9	-2.9303586836893807
2216	10	-2.472268839823524
2216	11	-2.966727255827003
2216	12	-3.5266555658116943
2216	13	-4.204856175378776
2216	14	-3.478381573815522
2216	15	-3.296968179326207
2216	16	-2.544733514253874
2216	17	-2.5718744720884317
2216	18	-2.5828119904735836
2216	19	-2.921628143403165
2216	20	-2.8577595845258124
2216	21	-3.2118724177083777
2216	22	-3.8676833376889554
2216	23	-3.7190408483197537
2216	24	-3.6211667391506914
2216	25	-3.612922435508132
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	119657.0
3	7.0
4	990.0
5	5678.0
6	7008.0
7	6288.0
8	5494.0
9	5224.0
10	4857.0
11	4791.0
12	4517.0
13	4445.0
14	4358.0
15	4537.0
16	4607.0
17	4861.0
18	5434.0
19	6470.0
20	8276.0
21	10599.0
22	13207.0
23	15871.0
24	18334.0
25	20856.0
26	23391.0
27	25618.0
28	29177.0
29	34236.0
30	42415.0
31	52687.0
32	66157.0
33	83534.0
34	109587.0
35	143207.0
36	195044.0
37	301570.0
38	799748.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78905568821083	18.131862850771743	12.365775214075402	25.713306246942025
2	15.476536455327402	20.104779211322686	39.671290924959635	24.747393408390277
3	18.49558111567933	24.542509064962985	29.71350702295119	27.248402796406495
4	12.56615657500893	16.664180306292227	37.725933742373826	33.04372937632502
5	13.617081460866393	37.354928485069685	35.51219289641171	13.515797157652212
6	32.10092558298226	36.61257245656255	18.05602942598529	13.230472534469891
7	27.90636044014589	31.451757098729953	22.43642022948744	18.20546223163672
8	25.779043676076206	35.38443221830786	20.603848085513825	18.23267602010212
9	26.59467758864413	15.059684519793803	19.76356090037892	38.582076991183136
10	15.119705441504758	28.395044834233303	34.25665671364021	22.22859301062173
11	34.433429745525636	22.455723905647115	24.147855950058453	18.962990398768795
12	23.101677651370185	24.66948958874529	31.17747598288731	21.051356776997217
13	28.774503431335656	21.63258138161151	25.53479344704978	24.058121740003056
14	21.771467516009572	21.242346470960154	27.37534247575185	29.610843537278424
15	23.689786757477403	29.144406860072852	23.426751911975018	23.739054470474727
16	23.326098836185622	27.56735700008302	26.179893571697697	22.92665059203366
17	22.609856662787838	27.60679358994418	26.94441264038704	22.838937106880945
18	22.440077596995103	27.182735079494	28.043807877583387	22.333379445927505
19	23.73035524325468	26.8012141294451	27.101005935622307	22.367424691677904
20	23.812847218896096	26.759056143918485	27.284044044427596	22.14405259275782
21	23.788957010437194	26.37791037150573	26.82372637033793	23.009406247719145
22	23.699652145830537	26.738883126290315	26.774781530613083	22.786683197266065
23	23.270434226893848	26.680095847126296	26.84417452278285	23.205295403197002
24	23.27856783485235	26.666855203877905	27.1175655710374	22.937011390232342
25	23.470114344284543	26.451586711120456	26.9946844299367	23.0836145146583
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	346.0
1	346.0
2	545.0
3	744.0
4	744.0
5	744.0
6	1499.5
7	2255.0
8	2255.0
9	2255.0
10	3297.0
11	4339.0
12	4339.0
13	4339.0
14	7140.5
15	9942.0
16	9942.0
17	9942.0
18	17401.5
19	24861.0
20	24861.0
21	24861.0
22	37694.5
23	50528.0
24	50528.0
25	50528.0
26	70611.0
27	90694.0
28	90694.0
29	90694.0
30	114921.5
31	139149.0
32	139149.0
33	139149.0
34	161963.5
35	184778.0
36	184778.0
37	184778.0
38	205010.5
39	225243.0
40	225243.0
41	225243.0
42	242027.5
43	258812.0
44	258812.0
45	258812.0
46	271830.5
47	284849.0
48	284849.0
49	284849.0
50	281255.5
51	277662.0
52	277662.0
53	277662.0
54	256253.0
55	234844.0
56	234844.0
57	234844.0
58	205345.0
59	175846.0
60	175846.0
61	175846.0
62	145338.0
63	114830.0
64	114830.0
65	114830.0
66	89648.0
67	64466.0
68	64466.0
69	64466.0
70	47300.5
71	30135.0
72	30135.0
73	30135.0
74	21026.5
75	11918.0
76	11918.0
77	11918.0
78	8190.0
79	4462.0
80	4462.0
81	4462.0
82	2932.5
83	1403.0
84	1403.0
85	1403.0
86	933.5
87	464.0
88	464.0
89	464.0
90	291.0
91	118.0
92	118.0
93	118.0
94	74.5
95	31.0
96	31.0
97	31.0
98	24.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3591858029485524
2	0.04058854299444028
3	0.03561758660523355
4	0.035480771291769145
5	0.04045172768097588
6	0.017740385645884572
7	0.01701070397407441
8	0.02417070537871163
9	0.020978348064542168
10	0.02002064087029133
11	0.018880513258087952
12	0.020339876601708277
13	0.020157456183755734
14	0.027864718842250575
15	0.02882242603650141
16	0.021115163378006573
17	0.022528921617138764
18	0.11155008557797857
19	0.025037202363986193
20	0.022620131826115032
21	0.026314145289653982
22	0.02002064087029133
23	0.022620131826115032
24	0.019154143885016763
25	0.022711342035091304
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2192737.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.6348370993395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.00913514168067	59.56720171673695
2	12.043423179162541	17.49544161473761
3	3.2118740467141	6.99881844500025
4	1.1814112655609785	3.432464592853848
5	0.533445179878833	1.9373351870963451
6	0.297706577765427	1.2974321267636182
7	0.17545236893252888	0.8920767965275217
8	0.12188239356351589	0.708232624141085
9	0.07356739665679585	0.4809200284789975
>10	0.3273939520640291	4.029964522833777
>50	0.014693167871369171	0.7276112552852646
>100	0.008379674380475844	1.1620123845121475
>500	8.807377216366311E-4	0.3877174745438937
>1k	7.549180471151969E-4	0.8827712304887646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4058	0.18506551401285243	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2560	0.11674906748962598	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2490	0.11355671017545652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.5605104488135146E-5
7	0.0	0.0	0.0	0.0	4.5605104488135146E-5
8	0.0	0.0	0.0	0.0	4.5605104488135146E-5
9	0.0	0.0	0.0	4.5605104488135146E-5	4.5605104488135146E-5
10	0.0	0.0	0.0	4.5605104488135146E-5	4.5605104488135146E-5
11	0.0	0.0	0.0	4.5605104488135146E-5	4.5605104488135146E-5
12	0.0	0.0	0.0	4.5605104488135146E-5	1.8242041795254058E-4
13	0.0	0.0	0.0	4.5605104488135146E-5	1.8242041795254058E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	115	7.133349E-8	11.558761	10
GGTATCA	1265	0.0	11.150131	1
AAGGCGT	145	1.2296368E-9	11.131206	6
AGGCGTG	190	9.094947E-12	10.493659	7
ATAAGCC	175	2.3646862E-10	10.311115	3
TGGCGAG	840	0.0	10.294609	18
GTGTTAT	200	2.3646862E-11	10.0068655	1
GCGTGCG	105	4.131569E-5	9.946825	9
ATGGCGA	905	0.0	9.870226	17
TATGGCG	915	0.0	9.658499	16
GGCGAGA	590	0.0	9.655623	19
GTATTAC	160	6.3184416E-8	9.530349	1
GTACTAA	160	6.3184416E-8	9.530349	1
GTCCTAT	190	9.931682E-10	9.530348	1
GTATCAA	2290	0.0	9.405497	1
GTGTTAG	300	0.0	9.21267	1
GGCGTGC	155	4.2958527E-7	9.1884165	8
GTCCTAC	790	0.0	9.168437	1
GTCTAGA	220	1.5825208E-10	9.097151	1
TAGGACC	355	0.0	9.095795	4
>>END_MODULE