FastQCFastQC Report
Thu 26 May 2016
SRR1512941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192737
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48910.22305456605146903No Hit
TATCAACGCAGAGTACTTTTTTTTT37890.17279774090554406No Hit
GGTATCAACGCAGAGTACTTTTTTT32030.1460731496754969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.012.377681
GTCTTAG2450.011.6396751
GCGTGCG752.0708518E-411.4021929
CCCTATA1201.2638338E-711.0910372
TAGGACC3200.010.7047124
AGTACCG803.716325E-410.7047125
TAATACC1803.45608E-1110.5725554
GTCCTAG2450.010.0877181
CCCCTAT856.5587537E-410.0648951
CCAACGA856.666987E-410.04699119
GTATCAA24300.09.7404441
GCAATAC1201.685547E-59.5072553
AGGCGTG1804.147296E-99.5048667
GACGTGA4650.09.4026637
GTCCTAA4250.09.3939021
CTAGACT2650.09.3357654
ATAGCAC2151.05501385E-109.2861563
AGGACCG1856.51562E-99.2581295
AAGGCGT1651.04459104E-79.2242156
GTAGGAC7850.09.2044763
Produced by FastQC (version 0.11.5)