##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512941_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2192737 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.278186576867174 31.0 31.0 34.0 26.0 34.0 2 30.432866777912718 31.0 31.0 34.0 27.0 34.0 3 30.54303730907993 31.0 31.0 34.0 27.0 34.0 4 34.02065546392477 37.0 35.0 37.0 30.0 37.0 5 33.64112203150674 37.0 35.0 37.0 28.0 37.0 6 33.49368620130914 37.0 35.0 37.0 28.0 37.0 7 33.35991502856932 37.0 35.0 37.0 27.0 37.0 8 33.244682330803926 37.0 35.0 37.0 27.0 37.0 9 34.68339796336724 39.0 35.0 39.0 27.0 39.0 10 34.56964195888517 39.0 35.0 39.0 27.0 39.0 11 34.59535867730603 39.0 35.0 39.0 27.0 39.0 12 34.43783226168939 39.0 35.0 39.0 27.0 39.0 13 34.41248950512533 39.0 35.0 39.0 26.0 39.0 14 35.46433977262207 40.0 36.0 41.0 26.0 41.0 15 35.46683619604175 40.0 36.0 41.0 26.0 41.0 16 35.43923553075449 40.0 36.0 41.0 26.0 41.0 17 35.41024527793347 40.0 36.0 41.0 26.0 41.0 18 35.333227377473904 40.0 36.0 41.0 25.0 41.0 19 35.31410150875367 40.0 36.0 41.0 25.0 41.0 20 35.28676124861303 40.0 36.0 41.0 25.0 41.0 21 35.23792319826774 40.0 36.0 41.0 25.0 41.0 22 35.19154919171793 40.0 36.0 41.0 25.0 41.0 23 35.0883480326186 40.0 35.0 41.0 24.0 41.0 24 35.06467214262358 40.0 35.0 41.0 24.0 41.0 25 34.97276326344655 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5850622463633037 1101 2 0.6481585205642197 1101 3 0.6371556506375953 1101 4 0.49098828214295764 1101 5 0.7475021717429797 1101 6 0.7679475424303703 1101 7 0.762692034276995 1101 8 0.7911910192284495 1101 9 0.5634333823593494 1101 10 0.8946284790187917 1101 11 0.9323234131286497 1101 12 1.0185523594876784 1101 13 0.9855417942222999 1101 14 1.1273930438007937 1101 15 1.0717123667053272 1101 16 1.227992264170581 1101 17 1.2209564230516676 1101 18 0.6163067977946213 1101 19 0.9397140414673544 1101 20 0.9706724513356448 1101 21 1.0512162265749225 1101 22 0.8834808973236292 1101 23 0.9946292201653506 1101 24 0.9203343425353907 1101 25 1.0606981228653112 1102 1 0.5805681211008284 1102 2 0.4877007669151361 1102 3 0.5132304232509775 1102 4 0.44431614132855657 1102 5 0.6358450180546313 1102 6 0.613469362086839 1102 7 0.6883752644486236 1102 8 0.6112271317712228 1102 9 0.8293726753051089 1102 10 0.9211125960316267 1102 11 0.8488862960285459 1102 12 0.875254741675036 1102 13 1.0180528710468053 1102 14 1.1700414481836958 1102 15 1.1490838280630982 1102 16 1.0364981786103158 1102 17 0.9333313084961645 1102 18 1.0787072518268772 1102 19 1.149883899221848 1102 20 1.0655844212951635 1102 21 1.1151479417048122 1102 22 0.9676557070594924 1102 23 1.0446034137302576 1102 24 0.9242195786382013 1102 25 1.1260631425856005 1103 1 0.47765128312239113 1103 2 0.42258377165084937 1103 3 0.45255116111278326 1103 4 0.4995675221735709 1103 5 0.4798677935827058 1103 6 0.6076952646385223 1103 7 0.6214983002527958 1103 8 0.5842424138706903 1103 9 0.7213959929057339 1103 10 0.7589816643082088 1103 11 0.7524630207618799 1103 12 0.7516034519293058 1103 13 0.7204401105034677 1103 14 0.9256635515276699 1103 15 0.7571075089300763 1103 16 0.8557073534179338 1103 17 0.9794560639848697 1103 18 1.0315260909329211 1103 19 0.9244908215307319 1103 20 0.9347697523313414 1103 21 0.7229973218639287 1103 22 0.8864977460933048 1103 23 1.032041836461687 1103 24 0.982409423824187 1103 25 0.8872340687681444 1104 1 0.4737383556995951 1104 2 0.4819751558162615 1104 3 0.4995650247398906 1104 4 0.4178161906385185 1104 5 0.4648271155342414 1104 6 0.4980313853576064 1104 7 0.5378181679322367 1104 8 0.45764445381263386 1104 9 0.5603709316059167 1104 10 0.6662370073608344 1104 11 0.5828419128219267 1104 12 0.5023964995062187 1104 13 0.5276932335298028 1104 14 0.6295065393823833 1104 15 0.59957680395636 1104 16 0.7049963244497377 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0.5955253434323495 1107 17 0.5332638274457437 1107 18 0.707948604462338 1107 19 0.691462171218312 1107 20 0.7564704533371298 1107 21 0.6755376318666535 1107 22 0.6119605438437858 1107 23 0.5800056727109038 1107 24 0.7386993396568613 1107 25 0.725747898371992 1108 1 0.35017240140087935 1108 2 0.21600580970543604 1108 3 0.2644156665979409 1108 4 0.37918191674670254 1108 5 0.40918707641780117 1108 6 0.3036862850512634 1108 7 0.31811361998420296 1108 8 0.399944823611726 1108 9 0.32825669775444055 1108 10 0.5150358141668718 1108 11 0.4894532902157138 1108 12 0.5129398667465708 1108 13 0.5079541411085629 1108 14 0.4853127452273611 1108 15 0.47885236185639 1108 16 0.5101488517159254 1108 17 0.4434873736908145 1108 18 0.45625133216764624 1108 19 0.5814341861899521 1108 20 0.596086777705537 1108 21 0.6871879747601284 1108 22 0.7706129703206699 1108 23 0.7404101808698087 1108 24 0.8146479554015613 1108 25 0.7789778163943382 1109 1 0.036267443376093667 1109 2 -0.04396126511249676 1109 3 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-2.0736540872983227 2216 18 -1.7373764178288909 2216 19 -2.033034615473973 2216 20 -2.53129359608495 2216 21 -2.3061764561230547 2216 22 -2.6642206598758946 2216 23 -1.7287886379200827 2216 24 -2.0024555541351035 2216 25 -1.8445927393454937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64213.0 3 2.0 4 1043.0 5 8867.0 6 10324.0 7 8035.0 8 6512.0 9 5738.0 10 4893.0 11 4407.0 12 4067.0 13 3749.0 14 3650.0 15 3636.0 16 3731.0 17 3860.0 18 4355.0 19 5106.0 20 6453.0 21 8458.0 22 10611.0 23 13076.0 24 15378.0 25 17605.0 26 20143.0 27 22038.0 28 24949.0 29 30112.0 30 37706.0 31 48542.0 32 61351.0 33 79192.0 34 103796.0 35 135387.0 36 184756.0 37 288723.0 38 938273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.89119173349277 19.019721498304435 13.367442412403413 27.72164435579938 2 14.234792836154696 20.550609672951516 38.41037645714275 26.80422103375103 3 16.724395128097896 24.546172203962445 30.170330504752734 28.55910216318692 4 11.801218717167195 17.18535367843732 37.031136731817895 33.982290872577586 5 13.284461921557458 37.600180004527495 35.48435092485085 13.631007149064194 6 32.238733216552454 37.118883723307825 17.58942728382226 13.052955776317459 7 27.65480528036543 31.583872383564692 22.325439429733674 18.4358829063362 8 25.559763228267286 35.858165169689606 20.371743852383258 18.210327749659854 9 26.299707446893468 14.858056299246552 19.58494969190886 39.25728656195112 10 14.724409592630103 28.767936411977914 34.24490593571612 22.262748059675868 11 34.447064875975954 22.379513992298637 24.054466867858647 19.118954263866765 12 22.833908670933653 24.805458652323367 31.188832368760995 21.17180030798199 13 28.671482403465582 21.54028170144682 25.62810884574652 24.16012704934108 14 21.573100842195046 21.10012155157276 27.414417886553387 29.91235971967881 15 23.491885250051425 29.263681596578135 23.476833863248572 23.767599290121865 16 23.171815371028764 27.66966730255697 26.100058297547395 23.058459028866864 17 22.479611519377496 27.624178575534554 27.013436597254287 22.882773307833666 18 22.420552214668866 27.26403727280273 27.961534146517213 22.35387636601119 19 23.52880813277327 26.789305895713326 27.257925486064554 22.42396048544885 20 23.73160420751517 26.679309462530664 27.280207137524155 22.308879192430012 21 23.605972283852676 26.20168748623291 27.03758649609687 23.15475373381754 22 23.460821406029382 26.63390078477586 26.924452098025835 22.98082571116892 23 23.030212925672345 26.532684950361123 27.04788581576359 23.389216308202947 24 23.1610189279512 26.526175517709383 27.282855756461334 23.029949797878086 25 23.294769137028243 26.413228734012854 27.11024841958551 23.181753709373393 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 276.0 1 276.0 2 461.5 3 647.0 4 647.0 5 647.0 6 1459.5 7 2272.0 8 2272.0 9 2272.0 10 3263.0 11 4254.0 12 4254.0 13 4254.0 14 7152.5 15 10051.0 16 10051.0 17 10051.0 18 17569.0 19 25087.0 20 25087.0 21 25087.0 22 38153.0 23 51219.0 24 51219.0 25 51219.0 26 71897.5 27 92576.0 28 92576.0 29 92576.0 30 116015.5 31 139455.0 32 139455.0 33 139455.0 34 162195.0 35 184935.0 36 184935.0 37 184935.0 38 205450.0 39 225965.0 40 225965.0 41 225965.0 42 243244.0 43 260523.0 44 260523.0 45 260523.0 46 272533.5 47 284544.0 48 284544.0 49 284544.0 50 281319.0 51 278094.0 52 278094.0 53 278094.0 54 256264.0 55 234434.0 56 234434.0 57 234434.0 58 204543.5 59 174653.0 60 174653.0 61 174653.0 62 144317.5 63 113982.0 64 113982.0 65 113982.0 66 88510.0 67 63038.0 68 63038.0 69 63038.0 70 46155.0 71 29272.0 72 29272.0 73 29272.0 74 20353.5 75 11435.0 76 11435.0 77 11435.0 78 7873.5 79 4312.0 80 4312.0 81 4312.0 82 2786.0 83 1260.0 84 1260.0 85 1260.0 86 812.5 87 365.0 88 365.0 89 365.0 90 219.5 91 74.0 92 74.0 93 74.0 94 43.0 95 12.0 96 12.0 97 12.0 98 7.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0787600154510094 2 1.8242041795254058E-4 3 0.0 4 0.03465987941098272 5 0.07666218064455518 6 0.07930727670486702 7 0.041911091024596205 8 0.08053861452604667 9 0.0611108400141011 10 0.12035187074418865 11 0.006293504419362651 12 0.018287646899742195 13 0.009257836211091436 14 0.011492486331010058 15 0.011310065913057516 16 0.024398730901152303 17 0.037076949948853875 18 0.0017785990750372707 19 0.005791848269993164 20 0.008391339225816868 21 3.6484083590508117E-4 22 4.104459403932164E-4 23 0.0 24 4.5605104488135146E-5 25 0.005290192120623678 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2192737.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.2414316473738 #Duplication Level Percentage of deduplicated Percentage of total 1 80.90700111300248 57.63930589585963 2 12.737980273695396 18.149439019881328 3 3.435251852460597 7.341967801157575 4 1.2473568039423064 3.5545393795176987 5 0.5599400548746634 1.9945465572990029 6 0.3109705239817951 1.3292391197158244 7 0.1922928860686939 0.9589454349397366 8 0.13058652329587137 0.7442536698760804 9 0.09188600464085242 0.5891481467074403 >10 0.36048510067909295 4.282962614133354 >50 0.015936236039343916 0.7766049445364764 >100 0.00871306669128638 1.205949473197648 >500 8.957539514847217E-4 0.42596374933271647 >1k 7.038066761646377E-4 1.0071341938455407 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4891 0.22305456605146903 No Hit TATCAACGCAGAGTACTTTTTTTTT 3789 0.17279774090554406 No Hit GGTATCAACGCAGAGTACTTTTTTT 3203 0.1460731496754969 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.5605104488135146E-5 2 0.0 0.0 0.0 0.0 4.5605104488135146E-5 3 0.0 0.0 0.0 0.0 4.5605104488135146E-5 4 0.0 0.0 0.0 0.0 4.5605104488135146E-5 5 0.0 0.0 0.0 0.0 4.5605104488135146E-5 6 0.0 0.0 0.0 0.0 4.5605104488135146E-5 7 0.0 0.0 0.0 0.0 9.121020897627029E-5 8 0.0 0.0 0.0 0.0 9.121020897627029E-5 9 0.0 0.0 0.0 0.0 9.121020897627029E-5 10 0.0 0.0 0.0 0.0 9.121020897627029E-5 11 0.0 0.0 0.0 0.0 9.121020897627029E-5 12 0.0 0.0 0.0 0.0 1.8242041795254058E-4 13 0.0 0.0 0.0 0.0 1.8242041795254058E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1175 0.0 12.37768 1 GTCTTAG 245 0.0 11.639675 1 GCGTGCG 75 2.0708518E-4 11.402192 9 CCCTATA 120 1.2638338E-7 11.091037 2 TAGGACC 320 0.0 10.704712 4 AGTACCG 80 3.716325E-4 10.704712 5 TAATACC 180 3.45608E-11 10.572555 4 GTCCTAG 245 0.0 10.087718 1 CCCCTAT 85 6.5587537E-4 10.064895 1 CCAACGA 85 6.666987E-4 10.046991 19 GTATCAA 2430 0.0 9.740444 1 GCAATAC 120 1.685547E-5 9.507255 3 AGGCGTG 180 4.147296E-9 9.504866 7 GACGTGA 465 0.0 9.402663 7 GTCCTAA 425 0.0 9.393902 1 CTAGACT 265 0.0 9.335765 4 ATAGCAC 215 1.05501385E-10 9.286156 3 AGGACCG 185 6.51562E-9 9.258129 5 AAGGCGT 165 1.04459104E-7 9.224215 6 GTAGGAC 785 0.0 9.204476 3 >>END_MODULE