FastQCFastQC Report
Thu 26 May 2016
SRR1512940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459100
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38350.15595136432027976No Hit
GTCCTACAGTGGACATTTCTAAATT35010.14236915944857875No Hit
GTCCTAAAGTGTGTATTTCTCATTT33030.13431743320727096No Hit
CTGTAGGACGTGGAATATGGCAAGA31540.12825830588426662No Hit
CTTTAGGACGTGAAATATGGCGAGG30060.12223984384530925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7550.012.4535784
GGTATCA11700.011.8945661
GGCGAGG8950.011.24468719
AGGACGT18800.010.9117155
CTAGTAC1408.394636E-910.8542013
GTATCAA25900.010.7832431
GGACGTG19350.010.6962436
GACCGGC803.7939157E-410.6809117
GCCGGTT1252.2595304E-710.63387211
TCCAACG1701.382432E-1010.62038818
TTAGGAC11600.010.4799193
TGTAGGA22450.010.4494962
TAGGACG20350.010.3608294
GGACCGT1106.09336E-610.3572466
GTAGGAC21250.010.2795673
GTCCTAC20900.010.1704491
AGGACCT15250.010.02658755
GACGTGA11050.09.96670157
TGGCGAG19600.09.793344518
TCCTACA22750.09.6437372
Produced by FastQC (version 0.11.5)