##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512940_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2459100
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.057519011020293	31.0	30.0	34.0	23.0	34.0
2	29.018956935464193	31.0	30.0	34.0	22.0	34.0
3	29.272114188117605	31.0	30.0	34.0	23.0	34.0
4	32.690045138465294	35.0	33.0	37.0	25.0	37.0
5	32.857080639258264	35.0	35.0	37.0	26.0	37.0
6	32.84255581310236	36.0	35.0	37.0	26.0	37.0
7	32.70385832215038	36.0	33.0	37.0	25.0	37.0
8	32.69848481151641	37.0	35.0	37.0	25.0	37.0
9	34.02535114472774	39.0	34.0	39.0	25.0	39.0
10	33.90511609938596	38.0	34.0	39.0	23.0	39.0
11	33.97616648367289	39.0	34.0	39.0	25.0	39.0
12	33.87204465048188	38.0	34.0	39.0	23.0	39.0
13	33.868015940791345	38.0	34.0	39.0	23.0	39.0
14	34.856569476637794	39.0	34.0	41.0	22.0	41.0
15	34.80282827050547	39.0	34.0	41.0	22.0	41.0
16	34.68254442682282	39.0	34.0	41.0	19.0	41.0
17	34.62191330161441	39.0	34.0	41.0	19.0	41.0
18	34.31409092757513	39.0	34.0	41.0	17.0	41.0
19	34.555065674433735	39.0	34.0	41.0	18.0	41.0
20	34.64474035216136	39.0	34.0	41.0	18.0	41.0
21	34.57842584685454	39.0	34.0	41.0	18.0	41.0
22	34.48652921800659	39.0	34.0	41.0	18.0	41.0
23	34.212133300801106	39.0	34.0	41.0	15.0	41.0
24	34.22368386808182	39.0	34.0	41.0	10.0	41.0
25	34.14254686673986	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4869132498040152
1101	2	0.7488511309774069
1101	3	0.8353394045457172
1101	4	0.8210801675691783
1101	5	0.5238014026596076
1101	6	0.7998180704763129
1101	7	0.8612278888905536
1101	8	0.644106489964777
1101	9	0.45474471341124456
1101	10	0.6072818921654388
1101	11	0.7280132111589808
1101	12	0.9525519351418055
1101	13	0.8477703600290809
1101	14	0.770198992848357
1101	15	0.6135335620327496
1101	16	1.0480301222865336
1101	17	1.0602417761173015
1101	18	1.319027989106786
1101	19	0.8239068439083468
1101	20	0.9943071966081973
1101	21	0.960876104627566
1101	22	0.975457479720518
1101	23	1.180905955836522
1101	24	0.8046986417680628
1101	25	0.9462824308967441
1102	1	0.6338234624734937
1102	2	0.6880636509233042
1102	3	0.6443066296640652
1102	4	0.7666864527023307
1102	5	0.7540388492191923
1102	6	0.7524787190891971
1102	7	0.9514037015293795
1102	8	0.8600116268491433
1102	9	0.816352945029216
1102	10	1.0517967820023415
1102	11	0.9585943947006612
1102	12	1.0254323238212066
1102	13	0.8954736766299263
1102	14	1.104935769946394
1102	15	1.108822671352975
1102	16	1.18543957057188
1102	17	1.010556378727614
1102	18	1.2918917091514928
1102	19	1.2612367802330198
1102	20	1.198015353117242
1102	21	1.0149883397199346
1102	22	1.2741403287818898
1102	23	1.479937259418108
1102	24	1.3145606552076146
1102	25	1.1982608663152234
1103	1	0.34499894776781304
1103	2	0.5145910980138915
1103	3	0.5568254934891961
1103	4	0.5556879390983624
1103	5	0.2423307456051802
1103	6	0.5166711424298498
1103	7	0.3892250858578876
1103	8	0.4654295639131405
1103	9	0.6226295271325881
1103	10	0.7050869460598079
1103	11	0.6832805192061997
1103	12	0.7861301794172917
1103	13	0.7331622491053764
1103	14	0.9853998401235629
1103	15	1.0575164596466422
1103	16	1.1480480003510962
1103	17	0.9096052459010835
1103	18	1.2297429349504796
1103	19	1.1909844197103894
1103	20	1.180424256316499
1103	21	0.98165057055472
1103	22	1.1340002829686497
1103	23	1.4063638409537305
1103	24	1.1798802829743877
1103	25	1.2112217577872642
1104	1	0.2840426894301089
1104	2	0.31888825026833345
1104	3	0.3336668875572997
1104	4	0.4586243703063033
1104	5	0.22239010148816618
1104	6	0.2535596374631197
1104	7	0.4373887662088052
1104	8	0.4151668217727291
1104	9	0.5843886781378274
1104	10	0.6757518642691664
1104	11	0.6246761748766687
1104	12	0.49402427201680155
1104	13	0.48938382069059116
1104	14	0.7019767593560502
1104	15	0.7383211606402114
1104	16	0.5893991292296192
1104	17	0.5631167608974152
1104	18	0.8161751615278661
1104	19	0.6491067845129805
1104	20	0.7378667136993826
1104	21	0.7436390260214836
1104	22	0.6441753873885787
1104	23	0.8643621303345412
1104	24	0.8309068827416226
1104	25	0.5594055995425222
1105	1	0.5899387526412738
1105	2	0.7661772042639257
1105	3	0.7434104031579984
1105	4	0.8266094002831608
1105	5	0.6203088062696409
1105	6	0.348956932846221
1105	7	0.7043866691068601
1105	8	0.7604969962018089
1105	9	0.5239322366715342
1105	10	0.74218571391701
1105	11	0.8362677213170144
1105	12	0.7094324097809235
1105	13	0.8256502770166918
1105	14	0.8017668593728828
1105	15	0.9773974922633428
1105	16	0.9696885264505752
1105	17	0.9087084376144361
1105	18	0.9772029563471278
1105	19	0.9405077040548022
1105	20	0.9464374274630316
1105	21	0.9599588971384065
1105	22	0.6711543167976686
1105	23	0.9487221719444463
1105	24	1.0751582212801907
1105	25	0.9906302605774684
1106	1	0.4800245489221382
1106	2	0.5818263901498959
1106	3	0.5973391242568766
1106	4	0.6784311900897322
1106	5	0.6573247401490647
1106	6	0.5903907928626069
1106	7	0.33140766973497904
1106	8	0.6048606115708779
1106	9	0.702473908937506
1106	10	0.8617946286074982
1106	11	0.7922065181782187
1106	12	0.8628091638564683
1106	13	0.8246926714685543
1106	14	1.0193067687288462
1106	15	1.0895151841751556
1106	16	1.0788336594353112
1106	17	1.0352903532037914
1106	18	1.268462366912047
1106	19	0.727161947730572
1106	20	0.8714981239269619
1106	21	0.8683907035786547
1106	22	0.9274294701190797
1106	23	1.1822029287514582
1106	24	1.0780352187388544
1106	25	1.1546844160579681
1107	1	0.6317728445935558
1107	2	0.6701052155683485
1107	3	0.7723423050260259
1107	4	0.7774991844658103
1107	5	0.7536284448190784
1107	6	0.7249390994855318
1107	7	0.8474311768268876
1107	8	0.835583137375373
1107	9	1.035586923678558
1107	10	0.7938620963448315
1107	11	0.8319727356719682
1107	12	1.0325801001621713
1107	13	0.9836812029839379
1107	14	1.202735847239893
1107	15	1.2610045410800055
1107	16	1.2264242469354514
1107	17	1.2326639236695058
1107	18	1.562408920327222
1107	19	1.479111235801959
1107	20	1.3418406323700935
1107	21	1.2295344276392797
1107	22	1.3577178008031083
1107	23	1.3095802517195168
1107	24	1.38192026654702
1107	25	1.4500652950454054
1108	1	0.5264465814857573
1108	2	0.5666730533581195
1108	3	0.45299768123666695
1108	4	0.5599441832732026
1108	5	0.4520994854158573
1108	6	0.6486458617846509
1108	7	0.7213013700423474
1108	8	0.6861069974499756
1108	9	0.8759556349288715
1108	10	0.7965620712661661
1108	11	0.6463483947131508
1108	12	0.72943248558866
1108	13	0.736271293328457
1108	14	0.9699063573115438
1108	15	0.8919741429618355
1108	16	0.8574485893789756
1108	17	0.934493375189092
1108	18	1.0349635971432747
1108	19	1.0279869724675805
1108	20	0.9943992533468915
1108	21	0.9038886358683769
1108	22	0.7913519382567884
1108	23	1.0522289912825755
1108	24	1.091706917144407
1108	25	1.1442852275169813
1109	1	0.12858404315117866
1109	2	0.05024808046250584
1109	3	0.2837797988565036
1109	4	0.32847856142739573
1109	5	0.2259659077284013
1109	6	0.13436907156496858
1109	7	0.32706400607346353
1109	8	0.3916182868199982
1109	9	0.44775018215393203
1109	10	0.6445115386853786
1109	11	0.2234384311659099
1109	12	0.5108180780100753
1109	13	0.45518928595723196
1109	14	0.3520756184494829
1109	15	0.6427424931057928
1109	16	0.448220851099542
1109	17	0.3157340349399078
1109	18	-1.1020321421838446
1109	19	0.23352639122163055
1109	20	0.5209913399938912
1109	21	0.4286017592742368
1109	22	0.5782417346279161
1109	23	0.1331468796203339
1109	24	0.5405052019950247
1109	25	0.64532435615358
1110	1	0.28615285128369905
1110	2	0.33713457779827394
1110	3	0.3826131653048783
1110	4	0.1675349450362731
1110	5	0.36227419518610304
1110	6	0.30425827050284227
1110	7	0.39751046862912887
1110	8	0.2011055995039115
1110	9	0.6225124786726468
1110	10	0.8063918186391277
1110	11	0.624692706530368
1110	12	0.11153461300123269
1110	13	0.3089839902638616
1110	14	0.5403070917815072
1110	15	0.6024231364472357
1110	16	0.6793603728758058
1110	17	0.3895799092979857
1110	18	0.8479981933207128
1110	19	0.7899985728590906
1110	20	0.7542254877976191
1110	21	0.7505975136335223
1110	22	0.8217620256359695
1110	23	0.6788121208773603
1110	24	0.8356372113119193
1110	25	0.8219591818447896
1111	1	-0.09766026586894228
1111	2	0.21992683967441806
1111	3	0.1313377091310315
1111	4	-0.00306114046386341
1111	5	-0.0051596770235207146
1111	6	0.17113070636160188
1111	7	0.1126186363839139
1111	8	-0.06783698647846137
1111	9	0.25813422584568
1111	10	0.11518548914572335
1111	11	0.003899580298309502
1111	12	0.20528338290127124
1111	13	0.1231920074714381
1111	14	0.058957143818567204
1111	15	0.276754980199037
1111	16	0.1998577711590528
1111	17	0.30153532999864296
1111	18	0.3877418122261105
1111	19	0.2591455886859535
1111	20	0.015440832689385786
1111	21	0.3489515734843778
1111	22	0.12065591975005674
1111	23	0.33645001840310584
1111	24	0.48092439445008495
1111	25	-0.11639287307082213
1112	1	-0.0689056377450008
1112	2	0.2545037839981603
1112	3	0.21650487762622816
1112	4	0.322793511627502
1112	5	0.2860974950377937
1112	6	0.2233405905586352
1112	7	-0.042032561233234844
1112	8	0.26620445348526545
1112	9	0.4447970418968552
1112	10	0.440157310540755
1112	11	0.3022031603951092
1112	12	0.45820673620882246
1112	13	0.10652588319302936
1112	14	0.31870994985909107
1112	15	0.3690375321419239
1112	16	0.4546099621517641
1112	17	0.4754236404537693
1112	18	0.5098298610113048
1112	19	0.3768587133287511
1112	20	0.41211085534074243
1112	21	0.37701863068423336
1112	22	0.34249892502582213
1112	23	0.49846493824187377
1112	24	0.24746363740025146
1112	25	0.4440987339996525
1113	1	-0.1606264852438919
1113	2	-0.033162625001246226
1113	3	-0.20585929949401915
1113	4	0.15967426785336158
1113	5	-0.11146443224426861
1113	6	0.0847886354178371
1113	7	-0.11221651547428735
1113	8	0.13246664695267896
1113	9	-0.019339041471816643
1113	10	-0.18089454949354433
1113	11	-0.1427643643015486
1113	12	0.04272010393452064
1113	13	-0.1617559940347988
1113	14	0.008575500131392744
1113	15	-0.3683408385870237
1113	16	-0.2639028628859208
1113	17	-0.3923706806554108
1113	18	-0.47579966206657787
1113	19	-0.4286689631293328
1113	20	-0.479325714993152
1113	21	-0.29221448422613605
1113	22	-0.18768343182428282
1113	23	-0.6381135153973716
1113	24	0.03470902168066203
1113	25	0.011836841022329736
1114	1	-1.4753441469180508
1114	2	-1.1944683456760252
1114	3	-1.183174237436024
1114	4	-1.2998335734642907
1114	5	-1.3415874929799152
1114	6	-1.786669366907077
1114	7	-1.829336449820854
1114	8	-1.3198630248276224
1114	9	-1.3843355209320833
1114	10	-1.5291226403445322
1114	11	-2.2360461285878195
1114	12	-1.7928127447508757
1114	13	-1.6177109289862344
1114	14	-1.9992146260804091
1114	15	-1.956861598670514
1114	16	-2.558672047687544
1114	17	-4.760280357428464
1114	18	-3.6749282641163887
1114	19	-2.501131628322028
1114	20	-1.7412029808662268
1114	21	-2.576344543777388
1114	22	-2.6602632454928
1114	23	-2.5522659112669324
1114	24	-2.036461385750343
1114	25	-1.8827457140462514
1115	1	-1.1742587420023725
1115	2	-0.7441697296867105
1115	3	-0.8292624180593293
1115	4	-0.8594647474376842
1115	5	-0.7886659201611508
1115	6	-0.8925686604048195
1115	7	-1.9024530928438672
1115	8	-1.2953158481212448
1115	9	-0.9455913713994235
1115	10	-0.9078874514720567
1115	11	-1.6892574148255903
1115	12	-1.2160244473277615
1115	13	-1.1610150683850122
1115	14	-1.1369468262717888
1115	15	-2.295980024115522
1115	16	-1.1334054288237851
1115	17	-1.1486527897285583
1115	18	-1.3131526779828135
1115	19	-1.2908193923881726
1115	20	-1.0994065000969044
1115	21	-1.8461365111854633
1115	22	-1.8142120924753655
1115	23	-1.2359909429301723
1115	24	-1.1517562468515052
1115	25	-1.5167105904252196
1116	1	-1.1007258482370048
1116	2	-1.8913212907522023
1116	3	-1.425396445627559
1116	4	-1.0023061241799596
1116	5	-1.5333524551286324
1116	6	-1.3682211748662638
1116	7	-1.0798794373889642
1116	8	-1.7517699214011202
1116	9	-1.700688412340881
1116	10	-2.6840356676264996
1116	11	-1.361161702860315
1116	12	-1.2616726602721187
1116	13	-1.8545899500932919
1116	14	-1.6452551102232746
1116	15	-1.3157690571730072
1116	16	-1.1528425079010773
1116	17	-1.4754245332554135
1116	18	-1.3003564350690766
1116	19	-1.8100887765008125
1116	20	-2.1759507126303674
1116	21	-2.4848631979258755
1116	22	-1.6955839554174759
1116	23	-1.6065314574429763
1116	24	-2.1477829290977937
1116	25	-1.9923435875554034
1201	1	0.3902732074144346
1201	2	0.34815834062622386
1201	3	0.5295105848846688
1201	4	0.5495298196801457
1201	5	0.6199228061954116
1201	6	0.5766536175398684
1201	7	0.4763174430044046
1201	8	0.10516224832111476
1201	9	0.32146014345709517
1201	10	0.5540238588966702
1201	11	0.112983987328235
1201	12	-0.43750645492514906
1201	13	-0.08807752702514193
1201	14	0.41706180012873517
1201	15	0.290623856956401
1201	16	0.5945963536704468
1201	17	0.6583095512149342
1201	18	1.0295588991977453
1201	19	0.6087031086143
1201	20	0.5683506322522405
1201	21	0.5048183286624166
1201	22	0.7076919571814315
1201	23	1.0128641594771466
1201	24	0.7539566260814681
1201	25	0.7601237672917094
1202	1	0.5207067975616688
1202	2	0.6073043251062735
1202	3	0.3471693155317652
1202	4	0.6619184562012137
1202	5	0.6432946411026705
1202	6	0.5230054032409726
1202	7	0.5177762856786714
1202	8	0.6411621751834744
1202	9	0.8253204393199312
1202	10	1.0497092657933393
1202	11	0.672494007360207
1202	12	0.6768690639389092
1202	13	0.7927413191372281
1202	14	0.9643003796692611
1202	15	0.5230957719282756
1202	16	0.831326637187793
1202	17	0.9387658738053588
1202	18	1.212793291608591
1202	19	1.00824431059619
1202	20	0.9453925172622917
1202	21	0.9783284745755196
1202	22	1.0062653864891615
1202	23	1.0805646453853157
1202	24	1.2262116879618858
1202	25	0.9956017094377287
1203	1	0.19814670234957532
1203	2	0.40566621044653317
1203	3	0.3945146882151036
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2211	15	0.40856937325793297
2211	16	0.30929574560580875
2211	17	0.45773987973586117
2211	18	0.31080747242491924
2211	19	0.13777676122192162
2211	20	-0.005263236325554033
2211	21	0.12703620811481642
2211	22	-0.06920331596341356
2211	23	0.5751935520350955
2211	24	0.2955495617400672
2211	25	0.3182326793471617
2212	1	-0.2169995471333408
2212	2	-0.19079860707796925
2212	3	-0.19629781337463825
2212	4	-0.31911321952364347
2212	5	-0.27411630076504423
2212	6	-0.35175688826221574
2212	7	-0.3808087081729923
2212	8	-0.2519585160347688
2212	9	-0.2407651456924853
2212	10	-0.2842133003527749
2212	11	-0.4480300852315722
2212	12	-0.2719551785212033
2212	13	-0.5084769427936635
2212	14	-0.6269838528426703
2212	15	-0.578998833699572
2212	16	-0.43208464148686687
2212	17	-0.4378067550347353
2212	18	-3.0526791137606253
2212	19	-1.3132595082081266
2212	20	-0.8089211403938918
2212	21	-0.8283563093636275
2212	22	-0.5826364480057364
2212	23	-0.6125874129974207
2212	24	-0.9892022469681976
2212	25	-0.6717446687980839
2213	1	-0.21854339654235844
2213	2	0.007791791287711902
2213	3	-0.33555799139886133
2213	4	-0.353788544198963
2213	5	-0.3138434275708235
2213	6	-0.2596687516796976
2213	7	-0.10533081527936616
2213	8	-0.5115703648600984
2213	9	-0.2619237623567159
2213	10	-0.4023980369644775
2213	11	-0.3408259410611336
2213	12	-0.442147794893593
2213	13	-0.26855574040055075
2213	14	-0.2550270456049688
2213	15	-0.5378723197640696
2213	16	-1.0151548297255957
2213	17	-0.5735049893846522
2213	18	-0.34521231090056403
2213	19	-0.28767509262370794
2213	20	-1.0656656318574846
2213	21	-0.7391457446474448
2213	22	-0.5694072053382868
2213	23	-1.0121000352066787
2213	24	-0.7418025679954852
2213	25	-0.8429105815370335
2214	1	-1.6289036130396788
2214	2	-3.5247356594987167
2214	3	-1.6733743643621324
2214	4	-1.30204921714952
2214	5	-1.2860852771249682
2214	6	-1.3368055322005894
2214	7	-1.5947617325666492
2214	8	-1.2706602847334771
2214	9	-1.4786825204647656
2214	10	-1.5796294997345726
2214	11	-1.1835272114990758
2214	12	-1.6640726708881104
2214	13	-1.6093557347478793
2214	14	-2.7769653466945172
2214	15	-2.952292850267302
2214	16	-2.2329998899972026
2214	17	-2.6472618962427568
2214	18	-1.9521526610835664
2214	19	-4.156023381983971
2214	20	-2.4778708803630565
2214	21	-2.4707683440284782
2214	22	-2.501315264634812
2214	23	-2.594903760317255
2214	24	-2.711366888332382
2214	25	-2.640795775776958
2215	1	-0.7258080263969156
2215	2	-2.3685820187551663
2215	3	-1.1066512466647787
2215	4	-0.6492551665720612
2215	5	-0.6385066407232429
2215	6	-1.1519615321739138
2215	7	-1.5533851856358964
2215	8	-1.5863997135023986
2215	9	-1.1418345523394535
2215	10	-2.681167783072354
2215	11	-1.6705754401631552
2215	12	-1.91645524071183
2215	13	-1.2592034300410404
2215	14	-2.577122754319383
2215	15	-2.337358029897267
2215	16	-2.0492018431970678
2215	17	-2.183995367644812
2215	18	-1.0633825020203957
2215	19	-1.5915035796176653
2215	20	-1.7992884651925465
2215	21	-1.479935692775527
2215	22	-1.1227336178013942
2215	23	-1.6830689916432604
2215	24	-1.6030438260712714
2215	25	-1.421388059295083
2216	1	-1.4356313046774822
2216	2	-1.2898864418481857
2216	3	-1.8636477597154837
2216	4	-2.6809450700787814
2216	5	-1.816727907901889
2216	6	-1.7330490406073338
2216	7	-1.599513551561813
2216	8	-2.2725703408798594
2216	9	-2.4550589047137024
2216	10	-1.8912746997372096
2216	11	-2.5016339381286947
2216	12	-2.7925829677397367
2216	13	-3.383475629269263
2216	14	-2.744506160528559
2216	15	-2.562895563468402
2216	16	-1.7302530630670319
2216	17	-1.8641065850983551
2216	18	-1.980768772376308
2216	19	-2.3862613546866314
2216	20	-2.207449898764409
2216	21	-2.4918832261055854
2216	22	-2.932541833832982
2216	23	-2.7926128697738477
2216	24	-2.833745960925423
2216	25	-2.907900458449884
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	119309.0
3	4.0
4	1042.0
5	5634.0
6	7041.0
7	6412.0
8	5597.0
9	5328.0
10	5000.0
11	4692.0
12	4575.0
13	4493.0
14	4694.0
15	4571.0
16	4786.0
17	5078.0
18	5655.0
19	6652.0
20	8587.0
21	11210.0
22	13896.0
23	17271.0
24	19838.0
25	22377.0
26	25410.0
27	28347.0
28	32183.0
29	38262.0
30	46764.0
31	58678.0
32	73836.0
33	94733.0
34	123184.0
35	161283.0
36	220145.0
37	342597.0
38	919936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.077118968825026	18.618137712823287	12.634415513659174	25.670327804692512
2	14.857390434114015	20.09153373933632	41.124156364035784	23.926919462513883
3	18.398599646250403	24.788425521390135	29.90595695449908	26.907017877860383
4	12.265059329345906	16.81699859288829	38.40483405316108	32.513108024604726
5	13.190466949893434	37.780546853038175	35.95975104359015	13.069235153478234
6	31.547152283834524	37.166079900043606	18.33794829078462	12.948819525337255
7	27.73929729716539	31.612232264656047	22.686948026541092	17.961522411637475
8	24.99438686689553	36.65318972389893	20.84770512340758	17.50471828579796
9	26.539808614736593	15.112740944952026	20.25281857446572	38.094631865845656
10	14.930271547854904	28.718759265910116	34.793454347559944	21.55751483867504
11	33.98369420430442	22.375749145359112	25.086887642940088	18.55366900739638
12	23.21077256034592	24.62982237575027	31.84924695469038	20.310158109213432
13	28.813841277433617	21.970481778983874	25.9094442119289	23.30623273165361
14	21.06502497518752	21.938709100079727	27.65172223035746	29.344543694375297
15	22.86399742273912	30.798999684350626	23.405035095654778	22.931967797255478
16	22.436017110641192	27.93161275155201	27.51145271654003	22.120917421266768
17	21.894300040999877	28.479783156209532	27.65006442837712	21.975852374413474
18	21.800195407914018	27.457620908646803	29.3695652173913	21.372618466047875
19	23.45983072577706	27.013182043005152	27.41989411412506	22.107093117092727
20	23.607353259392912	27.217277133406764	27.915288486412347	21.260081120787973
21	23.1002740318889	26.67403709538401	27.108575119596594	23.1171137531305
22	23.329897804542547	27.79740033238131	27.10908793625047	21.763613926825677
23	22.637447382796182	27.59779203997138	27.472922146672097	22.29183843056034
24	23.10912338813852	26.864724027188924	28.0491554708116	21.976997113860953
25	22.89586598295827	27.212754819603234	27.635239102024645	22.256140095413848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	258.0
1	258.0
2	467.5
3	677.0
4	677.0
5	677.0
6	1336.5
7	1996.0
8	1996.0
9	1996.0
10	3091.0
11	4186.0
12	4186.0
13	4186.0
14	7370.0
15	10554.0
16	10554.0
17	10554.0
18	19339.0
19	28124.0
20	28124.0
21	28124.0
22	44568.0
23	61012.0
24	61012.0
25	61012.0
26	87830.0
27	114648.0
28	114648.0
29	114648.0
30	147956.0
31	181264.0
32	181264.0
33	181264.0
34	207554.5
35	233845.0
36	233845.0
37	233845.0
38	254646.0
39	275447.0
40	275447.0
41	275447.0
42	289696.5
43	303946.0
44	303946.0
45	303946.0
46	310912.0
47	317878.0
48	317878.0
49	317878.0
50	307022.5
51	296167.0
52	296167.0
53	296167.0
54	267242.0
55	238317.0
56	238317.0
57	238317.0
58	205748.0
59	173179.0
60	173179.0
61	173179.0
62	141957.0
63	110735.0
64	110735.0
65	110735.0
66	85821.5
67	60908.0
68	60908.0
69	60908.0
70	44732.0
71	28556.0
72	28556.0
73	28556.0
74	19896.5
75	11237.0
76	11237.0
77	11237.0
78	7717.5
79	4198.0
80	4198.0
81	4198.0
82	2810.0
83	1422.0
84	1422.0
85	1422.0
86	908.0
87	394.0
88	394.0
89	394.0
90	246.0
91	98.0
92	98.0
93	98.0
94	66.0
95	34.0
96	34.0
97	34.0
98	27.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35659387580822255
2	0.040258631206538976
3	0.03513480541661584
4	0.03550079297303892
5	0.040624618762962056
6	0.01740474157211988
7	0.017160749867837825
8	0.023585864747265258
9	0.020291976739457523
10	0.019966654467081454
11	0.018909357081859215
12	0.01964133219470538
13	0.01964133219470538
14	0.026839087471025987
15	0.027489732015778128
16	0.020861290716115654
17	0.022609897930137044
18	0.10979626692692449
19	0.02456183156439348
20	0.022121914521572934
21	0.025497133097474686
22	0.019438005774470336
23	0.021999918669431906
24	0.018380708389248098
25	0.02224391037371396
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2459100.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.33246979749771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.54490255200028	58.88131768227952
2	11.665895525127299	16.643142804138133
3	3.0643792415997715	6.55769219098484
4	1.129245985632746	3.222076206563736
5	0.5094281616989849	1.816938447919381
6	0.28563139089234396	1.2224875538427233
7	0.18070826546431476	0.9023256821874002
8	0.11668612220686789	0.6658807430486825
9	0.08377890143096	0.5378540360392343
>10	0.3856863556401249	4.640423876553705
>50	0.018959878695018362	0.9238814572331906
>100	0.011843550323927332	1.669679355610958
>500	0.0019407671154131618	0.9365446492702869
>1k	9.133021719476152E-4	1.3797553143281527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3835	0.15595136432027976	No Hit
GTCCTACAGTGGACATTTCTAAATT	3501	0.14236915944857875	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3303	0.13431743320727096	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3154	0.12825830588426662	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3006	0.12223984384530925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.066528404700907E-5
2	0.0	0.0	0.0	0.0	4.066528404700907E-5
3	0.0	0.0	0.0	0.0	4.066528404700907E-5
4	0.0	0.0	0.0	0.0	4.066528404700907E-5
5	0.0	0.0	0.0	0.0	4.066528404700907E-5
6	0.0	0.0	0.0	0.0	8.133056809401814E-5
7	0.0	0.0	0.0	0.0	8.133056809401814E-5
8	0.0	0.0	0.0	0.0	8.133056809401814E-5
9	0.0	0.0	0.0	0.0	8.133056809401814E-5
10	0.0	0.0	0.0	4.066528404700907E-5	8.133056809401814E-5
11	0.0	0.0	0.0	4.066528404700907E-5	8.133056809401814E-5
12	0.0	0.0	0.0	4.066528404700907E-5	8.133056809401814E-5
13	0.0	0.0	0.0	4.066528404700907E-5	8.133056809401814E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	755	0.0	12.453578	4
GGTATCA	1170	0.0	11.894566	1
GGCGAGG	895	0.0	11.244687	19
AGGACGT	1880	0.0	10.911715	5
CTAGTAC	140	8.394636E-9	10.854201	3
GTATCAA	2590	0.0	10.783243	1
GGACGTG	1935	0.0	10.696243	6
GACCGGC	80	3.7939157E-4	10.680911	7
GCCGGTT	125	2.2595304E-7	10.633872	11
TCCAACG	170	1.382432E-10	10.620388	18
TTAGGAC	1160	0.0	10.479919	3
TGTAGGA	2245	0.0	10.449496	2
TAGGACG	2035	0.0	10.360829	4
GGACCGT	110	6.09336E-6	10.357246	6
GTAGGAC	2125	0.0	10.279567	3
GTCCTAC	2090	0.0	10.170449	1
AGGACCT	1525	0.0	10.0265875	5
GACGTGA	1105	0.0	9.9667015	7
TGGCGAG	1960	0.0	9.7933445	18
TCCTACA	2275	0.0	9.643737	2
>>END_MODULE