FastQCFastQC Report
Thu 26 May 2016
SRR1512940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459100
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48280.19633199137895976No Hit
TATCAACGCAGAGTACTTTTTTTTT36270.1474929852385019No Hit
GTCCTACAGTGGACATTTCTAAATT33630.1367573502500915No Hit
CTTTAGGACGTGAAATATGGCGAGG31790.12927493798544182No Hit
CTGTAGGACGTGGAATATGGCAAGA31630.12862429344068968No Hit
GTCCTAAAGTGTGTATTTCTCATTT31470.12797364889593754No Hit
GGTATCAACGCAGAGTACTTTTTTT29310.11918994754178357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT655.3878182E-513.1712316
GGACGTC604.0481723E-412.6834076
TAGGACC6800.012.5945124
GACGGGT550.003058178412.096787
GGTTTCG550.003060044712.0957958
ACTGCGC752.0669788E-411.4046078
GGTATCA11350.011.0576511
AATACCG700.001471326310.8752615
GGCGAGG9500.010.78832519
GTATCAA23700.010.7515551
TGGCGAG19950.010.75005318
GTAGGAC19350.010.6136913
TGTAGGA20700.010.5639092
GACGTGG9750.010.2357377
AGGACGT20050.010.1091115
GGACGTG19900.09.9427726
AGGACCT14000.09.9236755
CTGTAGG21750.09.8794781
GGCGAGA11750.09.85316819
CAGCGCT1554.0547093E-89.8082189
Produced by FastQC (version 0.11.5)